MD10G1211300.v1.1

Ribosome biogenesis protein NSA2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002786
Physical Location & Seq
Forward (+)
31048151 .. 31051536
3386 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1211300.v1.1.491

Sequence Viewer

Length: 783 bp
ATGCCGCAGGGAGATTACATAGACCTTCACAGGAAGCGGTATGGGTACCGACCTGACCACTTCGAGCGAAAGCGCAAGAAGGAGGCTCGTCTCGTCCATAAGCGTTCTGAGACTGCTCAGAAGGCCCTAGGTATCAAGGGTAAGATGTTTGCTAAGAAGCGCTATGCTGAAAAGGCTCTCATGAAGAAAACGTTGATTATGCATGAGGAGTCATCAACTAGGCGCAAGGTGGATGATGATGTTCATGAAGGCTCTATTCCCGCATATTTGATCGATCGTGTCAATACAACACGTGCGAAAATTCTGAACAACACCATTAAGCAAAAGCGGAAGGAGAAAGCTGGGAAATGGGAAGTCCCACTGCCCAAGGTGAGGCCTGTGGCTGAAGATGAGATGTTCAAAATTCTTAGATCTGGAAAACGCAAGACCAAGCAATGGAAGAGGATGGTCACAAAAGCTACATTGGTGGGACCTGGTTTCACGAGGAAACCTCCTAAGTATGAGCGATTTATCCGTCCCAGTGGATTGCGATTCACCAAAGCTCACGTGACACACCCTGAACTTAAAGCCACTTTTAACCTTGAGATCATTGGTGTGAAGAAGAATCCTAATGGTCAAATGTATACATCTCTTGGTGTGTTGACCAAGGGAACCATCATTGAGGTGAATGTCAGTGAACTCGGTCTAGTCACACCTGCTGGGAAAGTTGTATGGGGGAAGTATGCTCAGGTGACAAATAATCCAGAGAACGACGGGTGTGTTAATGCAGTTCTGCTCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S8e

Protein Analysis

261

Amino Acids

29.79

Weight (kDa)

10.27

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 35 - 259 7.9e-40 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 703
Acc36I ACCTGC 1 cut(s) 703
Acc65I GGTACC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 45
AccB7I CCANNNNNTGG 1 cut(s) 435
AccI GTMKAC 1 cut(s) 623
AciI CCGC 4 cut(s) 5, 37, 261, 328
AclI AACGTT 1 cut(s) 191
AcsI RAATTY 2 cut(s) 300, 402
AcuI CTGAAG 1 cut(s) 405
AcvI CACGTG 2 cut(s) 293, 547
AfaI GTAC 1 cut(s) 47
AfeI AGCGCT 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 372, 435
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 1 cut(s) 400
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 3 cut(s) 341, 458, 542
AluI AGCT 3 cut(s) 341, 458, 542
Alw26I GTCTC 2 cut(s) 95, 104
Aor51HI AGCGCT 1 cut(s) 161
AoxI GGCC 2 cut(s) 123, 374
ApoI RAATTY 2 cut(s) 300, 402
Asp718I GGTACC 1 cut(s) 45
AspA2I CCTAGG 1 cut(s) 127
AspLEI GCGC 3 cut(s) 75, 162, 225
AspS9I GGNCC 2 cut(s) 124, 470
AsuHPI GGTGA 4 cut(s) 382, 526, 676, 742
AvaII GGWCC 1 cut(s) 470
AvrII CCTAGG 1 cut(s) 127
BanI GGYRCC 1 cut(s) 45
BauI CACGAG 1 cut(s) 481
BbrPI CACGTG 2 cut(s) 293, 547
BccI CCATC 2 cut(s) 439, 662
BciT130I CCWGG 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 95, 104
BfaI CTAG 3 cut(s) 128, 219, 686
BfoI RGCGCY 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 703
BglII AGATCT 1 cut(s) 410
BisI GCNGC 1 cut(s) 5
BlnI CCTAGG 1 cut(s) 127
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 474
Bme18I GGWCC 1 cut(s) 470
BmgT120I GGNCC 2 cut(s) 124, 470
BmiI GGNNCC 3 cut(s) 47, 471, 652
BmrFI CCNGG 1 cut(s) 474
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
BplI GAGNNNNNCTC 2 cut(s) 475, 507
Bpu10I CCTNAGC 1 cut(s) 726
BpuEI CTTGAG 1 cut(s) 602
Bsa29I ATCGAT 1 cut(s) 273
BsaAI YACGTR 2 cut(s) 293, 547
BsaJI CCNNGG 3 cut(s) 127, 366, 645
Bsc4I CCNNNNNNNGG 2 cut(s) 372, 435
Bse1I ACTGG 1 cut(s) 519
Bse3DI GCAATG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 474
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 3 cut(s) 127, 366, 645
BseGI GGATG 2 cut(s) 238, 450
BseLI CCNNNNNNNGG 2 cut(s) 372, 435
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 3 cut(s) 99, 131, 740
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 221
BseYI CCCAGC 2 cut(s) 341, 698
Bsh1285I CGRYCG 1 cut(s) 277
BshFI GGCC 2 cut(s) 125, 376
BshNI GGYRCC 1 cut(s) 45
BshVI ATCGAT 1 cut(s) 273
BsiEI CGRYCG 1 cut(s) 277
BslFI GGGAC 3 cut(s) 341, 483, 501
BslI CCNNNNNNNGG 2 cut(s) 372, 435
BsmAI GTCTC 2 cut(s) 95, 104
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 3 cut(s) 341, 483, 501
BsnI GGCC 2 cut(s) 125, 376
Bsp143I GATC 4 cut(s) 270, 274, 410, 585
BspACI CCGC 4 cut(s) 5, 37, 261, 328
BspANI GGCC 2 cut(s) 125, 376
BspCNI CTCAG 3 cut(s) 100, 130, 739
BspDI ATCGAT 1 cut(s) 273
BspHI TCATGA 2 cut(s) 180, 244
BspLI GGNNCC 3 cut(s) 47, 471, 652
BspMI ACCTGC 1 cut(s) 703
BspT107I GGYRCC 1 cut(s) 45
BsrDI GCAATG 1 cut(s) 440
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 3 cut(s) 127, 366, 645
BssMI GATC 4 cut(s) 270, 274, 410, 585
BssNAI GTATAC 1 cut(s) 624
BssSI CACGAG 1 cut(s) 481
BssT1I CCWWGG 3 cut(s) 127, 366, 645
Bst1107I GTATAC 1 cut(s) 624
Bst2BI CACGAG 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 474
Bst6I CTCTTC 1 cut(s) 434
BstBAI YACGTR 2 cut(s) 293, 547
BstDEI CTNAG 6 cut(s) 108, 117, 153, 407, 495, 726
BstF5I GGATG 2 cut(s) 238, 450
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 3 cut(s) 75, 162, 225
BstKTI GATC 4 cut(s) 273, 277, 413, 588
BstMAI GTCTC 2 cut(s) 95, 104
BstMBI GATC 4 cut(s) 270, 274, 410, 585
BstMCI CGRYCG 1 cut(s) 277
BstMWI GCNNNNNNNGC 2 cut(s) 122, 173
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 1 cut(s) 472
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
BstZ17I GTATAC 1 cut(s) 624
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 125, 376
BsuTUI ATCGAT 1 cut(s) 273
BtsCI GGATG 2 cut(s) 238, 450
BtsI GCAGTG 1 cut(s) 359
BtsIMutI CAGTG 3 cut(s) 359, 526, 679
BveI ACCTGC 1 cut(s) 703
CciI TCATGA 2 cut(s) 180, 244
CfoI GCGC 3 cut(s) 75, 162, 225
Cfr13I GGNCC 2 cut(s) 124, 470
ClaI ATCGAT 1 cut(s) 273
CsiI ACCWGGT 1 cut(s) 472
Csp6I GTAC 1 cut(s) 46
CviAII CATG 3 cut(s) 181, 203, 245
CviQI GTAC 1 cut(s) 46
DdeI CTNAG 6 cut(s) 108, 117, 153, 407, 495, 726
DpnI GATC 4 cut(s) 272, 276, 412, 587
DpnII GATC 4 cut(s) 270, 274, 410, 585
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Eco130I CCWWGG 3 cut(s) 127, 366, 645
Eco147I AGGCCT 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 470
Eco47III AGCGCT 1 cut(s) 161
Eco57I CTGAAG 1 cut(s) 405
Eco72I CACGTG 2 cut(s) 293, 547
EcoO109I RGGNCCY 2 cut(s) 124, 470
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 3 cut(s) 127, 366, 645
EcoT22I ATGCAT 1 cut(s) 204
ErhI CCWWGG 3 cut(s) 127, 366, 645
Esp3I CGTCTC 1 cut(s) 95
FaeI CATG 3 cut(s) 184, 206, 248
FaqI GGGAC 3 cut(s) 341, 483, 501
FatI CATG 3 cut(s) 180, 202, 244
FauI CCCGC 1 cut(s) 268
FblI GTMKAC 1 cut(s) 623
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 245, 457
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 3 cut(s) 128, 219, 686
GlaI GCGC 3 cut(s) 74, 161, 224
GluI GCNGC 1 cut(s) 5
GsaI CCCAGC 2 cut(s) 345, 702
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 2 cut(s) 125, 376
HhaI GCGC 3 cut(s) 75, 162, 225
Hin1II CATG 3 cut(s) 184, 206, 248
Hin6I GCGC 3 cut(s) 73, 160, 223
HinP1I GCGC 3 cut(s) 73, 160, 223
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HinfI GANTC 3 cut(s) 209, 531, 604
HphI GGTGA 4 cut(s) 382, 526, 676, 742
Hpy166II GTNNAC 3 cut(s) 624, 642, 677
Hpy188I TCNGA 3 cut(s) 109, 120, 306
Hpy188III TCNNGA 5 cut(s) 181, 245, 414, 481, 743
Hpy8I GTNNAC 3 cut(s) 624, 642, 677
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 5 cut(s) 35, 73, 115, 242, 325
HpyCH4IV ACGT 3 cut(s) 191, 292, 546
HpyCH4V TGCA 2 cut(s) 202, 767
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 173
HpyF3I CTNAG 6 cut(s) 108, 117, 153, 407, 495, 726
HpySE526I ACGT 3 cut(s) 191, 292, 546
Hsp92II CATG 3 cut(s) 184, 206, 248
HspAI GCGC 3 cut(s) 73, 160, 223
KpnI GGTACC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 270, 274, 410, 585
MabI ACCWGGT 1 cut(s) 472
MaeI CTAG 3 cut(s) 128, 219, 686
MaeII ACGT 3 cut(s) 191, 292, 546
MaeIII GTNAC 4 cut(s) 448, 547, 688, 730
MalI GATC 4 cut(s) 272, 276, 412, 587
MboI GATC 4 cut(s) 270, 274, 410, 585
MboII GAAGA 5 cut(s) 196, 398, 451, 610, 613
MflI RGATCY 1 cut(s) 410
MluCI AATT 2 cut(s) 300, 402
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 7 cut(s) 76, 199, 366, 435, 477, 501, 655
Mph1103I ATGCAT 1 cut(s) 204
MseI TTAA 5 cut(s) 318, 564, 576, 762, 781
MslI CAYNNNNRTG 2 cut(s) 593, 662
MspR9I CCNGG 1 cut(s) 474
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 2 cut(s) 122, 173
NdeII GATC 4 cut(s) 270, 274, 410, 585
NlaIII CATG 3 cut(s) 184, 206, 248
NlaIV GGNNCC 3 cut(s) 47, 471, 652
NmuCI GTSAC 4 cut(s) 448, 547, 688, 730
NsiI ATGCAT 1 cut(s) 204
PagI TCATGA 2 cut(s) 180, 244
PaqCI CACCTGC 1 cut(s) 703
PceI AGGCCT 1 cut(s) 376
PfeI GAWTC 2 cut(s) 531, 604
PflMI CCANNNNNTGG 1 cut(s) 435
PkrI GCNGC 1 cut(s) 6
Ple19I CGATCG 1 cut(s) 277
PleI GAGTC 1 cut(s) 217
PmaCI CACGTG 2 cut(s) 293, 547
PmlI CACGTG 2 cut(s) 293, 547
PpsI GAGTC 1 cut(s) 217
Ppu21I YACGTR 2 cut(s) 293, 547
PpuMI RGGWCCY 1 cut(s) 470
Psp1406I AACGTT 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 472
PspCI CACGTG 2 cut(s) 293, 547
PspFI CCCAGC 2 cut(s) 341, 698
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 3 cut(s) 47, 471, 652
PspPI GGNCC 2 cut(s) 124, 470
PspPPI RGGWCCY 1 cut(s) 470
PsuI RGATCY 1 cut(s) 410
PvuI CGATCG 1 cut(s) 277
RsaI GTAC 1 cut(s) 47
RsaNI GTAC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 593, 662
SaqAI TTAA 5 cut(s) 318, 564, 576, 762, 781
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 4 cut(s) 270, 274, 410, 585
Sau96I GGNCC 2 cut(s) 124, 470
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 474
SexAI ACCWGGT 1 cut(s) 472
SinI GGWCC 1 cut(s) 470
SmiMI CAYNNNNRTG 2 cut(s) 593, 662
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 2 cut(s) 300, 402
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 4 cut(s) 5, 37, 261, 328
SspMI CTAG 3 cut(s) 128, 219, 686
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 472
StyI CCWWGG 3 cut(s) 127, 366, 645
TaiI ACGT 3 cut(s) 194, 295, 549
TaqI TCGA 2 cut(s) 63, 273
TaqII GACCGA 1 cut(s) 671
TasI AATT 2 cut(s) 300, 402
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 2 cut(s) 531, 604
Tru1I TTAA 5 cut(s) 318, 564, 576, 762, 781
Tru9I TTAA 5 cut(s) 318, 564, 576, 762, 781
TscAI CASTG 3 cut(s) 366, 526, 679
TseFI GTSAC 4 cut(s) 448, 547, 688, 730
Tsp45I GTSAC 4 cut(s) 448, 547, 688, 730
TspDTI ATGAA 3 cut(s) 197, 233, 261
TspGWI ACGGA 1 cut(s) 503
TspRI CASTG 3 cut(s) 366, 526, 679
Van91I CCANNNNNTGG 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 470
XapI RAATTY 2 cut(s) 300, 402
XmaJI CCTAGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 623
XspI CTAG 3 cut(s) 128, 219, 686
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.