Rroxscaffold_3G00243120

Ribosome biogenesis protein NSA2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002786
Physical Location & Seq
Reverse (-)
35677276 .. 35678342
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243120.1

Sequence Viewer

Length: 483 bp
ATGCATGAGATGTCATCACTAGGCACAAGACTCTTAATTAGCAATTCCGTCACGCAAAAACGGAAAGAGAAAGCTGGAAAATGGGAAGTCCCTCTGCCTAAGGTGAGGCCTGTGGCTGAAGATGAGCTGTTTAAAGTGCTTAGAACGGGCACACGCAAGACTAAGCAATGGAAGCGGATGATCACAAAAGCTACATTTGTGGGACCTGGTTTTACAAGGAAACCTCCCAAGTATGAGCGATTTATCCGGCCCAGTGGATTGCGATTCACTAAAGCTCATGTGACTCACCCTGAACTCAAATGTACTTTTAACCTTGAGATCATCGGTGTGAAGAAAAACCCTAATGGTCAAATGCATACCTCTCTTGGTGTCTTGACCAAGGGAACCATCATTGAGGTGAATGTAAGTGAGCTTAGTCTAGTCACACCTAACGGGAAAGTTGTGTGGGGTGAGTTCAAAACCTCCGTGTACTTGGCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S8e

Protein Analysis

160

Amino Acids

18.13

Weight (kDa)

10.38

Isoelectric Point (pI)

32.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 17 - 148 9.6e-24 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 175
AcuI CTGAAG 1 cut(s) 138
AfaI GTAC 2 cut(s) 304, 470
AgsI TTSAA 1 cut(s) 457
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 5 cut(s) 74, 127, 191, 275, 412
AluI AGCT 5 cut(s) 74, 127, 191, 275, 412
AoxI GGCC 2 cut(s) 107, 248
AspS9I GGNCC 2 cut(s) 203, 249
AsuHPI GGTGA 4 cut(s) 115, 278, 409, 461
AvaII GGWCC 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 152
BccI CCATC 1 cut(s) 395
BciT130I CCWGG 1 cut(s) 207
BclI TGATCA 1 cut(s) 180
BfaI CTAG 2 cut(s) 20, 419
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 203, 249
BmiI GGNNCC 2 cut(s) 204, 385
BmrFI CCNGG 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 246
BpuEI CTTGAG 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 378
Bse1I ACTGG 1 cut(s) 252
Bse21I CCTNAGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 173
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 378
BseGI GGATG 1 cut(s) 183
BseMI GCAATG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 252
BseSI GKGCMC 1 cut(s) 152
BshFI GGCC 2 cut(s) 109, 250
BsiSI CCGG 1 cut(s) 247
BslFI GGGAC 2 cut(s) 74, 216
BsmFI GGGAC 2 cut(s) 74, 216
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 2 cut(s) 109, 250
Bsp1286I GDGCHC 1 cut(s) 152
Bsp143I GATC 2 cut(s) 180, 318
BspACI CCGC 1 cut(s) 175
BspANI GGCC 2 cut(s) 109, 250
BspLI GGNNCC 2 cut(s) 204, 385
BsrDI GCAATG 1 cut(s) 173
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 378
BssMI GATC 2 cut(s) 180, 318
BssT1I CCWWGG 1 cut(s) 378
Bst2UI CCWGG 1 cut(s) 207
BstDEI CTNAG 4 cut(s) 99, 140, 162, 413
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 2 cut(s) 183, 321
BstMBI GATC 2 cut(s) 180, 318
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNI CCWGG 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 99
BsuRI GGCC 2 cut(s) 109, 250
BtsCI GGATG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 203, 249
CsiI ACCWGGT 1 cut(s) 205
Csp6I GTAC 2 cut(s) 303, 469
CviAII CATG 2 cut(s) 5, 278
CviJI RGCY 8 cut(s) 74, 109, 116, 127, 191, 250, 275, 412
CviKI_1 RGCY 8 cut(s) 74, 109, 116, 127, 191, 250, 275, 412
CviQI GTAC 2 cut(s) 303, 469
DdeI CTNAG 4 cut(s) 99, 140, 162, 413
DpnI GATC 2 cut(s) 182, 320
DpnII GATC 2 cut(s) 180, 318
DraI TTTAAA 1 cut(s) 133
Eco130I CCWWGG 1 cut(s) 378
Eco147I AGGCCT 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 138
Eco81I CCTNAGG 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 378
EcoT22I ATGCAT 2 cut(s) 6, 357
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 2 cut(s) 8, 281
FaiI YATR 4 cut(s) 6, 234, 279, 357
FaqI GGGAC 2 cut(s) 74, 216
FatI CATG 2 cut(s) 4, 277
FbaI TGATCA 1 cut(s) 180
FokI GGATG 1 cut(s) 190
FspBI CTAG 2 cut(s) 20, 419
HaeIII GGCC 2 cut(s) 109, 250
HapII CCGG 1 cut(s) 247
Hin1II CATG 2 cut(s) 8, 281
HinfI GANTC 3 cut(s) 30, 264, 283
HpaII CCGG 1 cut(s) 247
HphI GGTGA 4 cut(s) 115, 278, 409, 461
Hpy166II GTNNAC 1 cut(s) 469
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 1 cut(s) 469
HpyCH4V TGCA 2 cut(s) 4, 355
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 4 cut(s) 99, 140, 162, 413
Hsp92II CATG 2 cut(s) 8, 281
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 318
LpnPI CCDG 7 cut(s) 60, 123, 192, 219, 260, 265, 303
MabI ACCWGGT 1 cut(s) 205
MaeI CTAG 2 cut(s) 20, 419
MaeIII GTNAC 3 cut(s) 49, 280, 421
MalI GATC 2 cut(s) 182, 320
MboI GATC 2 cut(s) 180, 318
MboII GAAGA 2 cut(s) 131, 343
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 2 cut(s) 36, 43
MlyI GAGTC 2 cut(s) 24, 277
MnlI CCTC 6 cut(s) 99, 102, 234, 370, 388, 472
Mph1103I ATGCAT 2 cut(s) 6, 357
MseI TTAA 3 cut(s) 35, 132, 309
MslI CAYNNNNRTG 2 cut(s) 326, 395
MspI CCGG 1 cut(s) 247
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 2 cut(s) 180, 318
NlaIII CATG 2 cut(s) 8, 281
NlaIV GGNNCC 2 cut(s) 204, 385
NmuCI GTSAC 3 cut(s) 49, 280, 421
NsiI ATGCAT 2 cut(s) 6, 357
PceI AGGCCT 1 cut(s) 109
PctI GAATGC 1 cut(s) 476
PfeI GAWTC 1 cut(s) 264
PleI GAGTC 2 cut(s) 24, 277
PpsI GAGTC 2 cut(s) 24, 277
PpuMI RGGWCCY 1 cut(s) 203
Psp5II RGGWCCY 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 204, 385
PspPI GGNCC 2 cut(s) 203, 249
PspPPI RGGWCCY 1 cut(s) 203
RsaI GTAC 2 cut(s) 304, 470
RsaNI GTAC 2 cut(s) 303, 469
RseI CAYNNNNRTG 2 cut(s) 326, 395
SaqAI TTAA 3 cut(s) 35, 132, 309
Sau3AI GATC 2 cut(s) 180, 318
Sau96I GGNCC 2 cut(s) 203, 249
SchI GAGTC 2 cut(s) 24, 277
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 152
SexAI ACCWGGT 1 cut(s) 205
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 2 cut(s) 326, 395
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 2 cut(s) 36, 43
SseBI AGGCCT 1 cut(s) 109
SsiI CCGC 1 cut(s) 175
SspMI CTAG 2 cut(s) 20, 419
StuI AGGCCT 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 378
TasI AATT 2 cut(s) 36, 43
TatI WGTACW 2 cut(s) 302, 468
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 3 cut(s) 35, 132, 309
Tru9I TTAA 3 cut(s) 35, 132, 309
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 3 cut(s) 49, 280, 421
Tsp45I GTSAC 3 cut(s) 49, 280, 421
TspGWI ACGGA 3 cut(s) 37, 76, 454
TspRI CASTG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 203
XspI CTAG 2 cut(s) 20, 419
Zsp2I ATGCAT 2 cut(s) 6, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.