AT5G06360

Ribosome biogenesis protein NSA2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002786
Physical Location & Seq
Forward (+)
1944754 .. 1946784
2031 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06360.1

Sequence Viewer

Length: 783 bp
ATGCCTCAGGGAGATTACATAGATTTGCACAGGAAGAGGAATGGATATCGCCTCGACCACTTCGAGAGAAAGCGCAAGAAGGAGGCGCGTGAAGTTCACAAGCACTCCACCATGGCTCAAAAGTCTCTAGGTATCAAGGGTAAGATGATTGCTAAGAAAAACTATGCTGAGAAAGCTCTCATGAAGAAAACATTGAAAATGCATGAAGAGTCATCATCAAGGCGTAAGGCTGATGAGAATGTTCAGGAAGGAGCTGTTCCTGCTTATCTTCTTGATCGTGAAGACACCACTCGTGCCAAGGTTCTTAGCAACACCATTAAACAAAAGAGGAAAGAGAAAGCTGGCAAATGGGAGGTCCCTCTTCCAAAGGTCCGTCCAGTTGCTGAAGATGAAATGTTTAGAGTGATCCGATCTGGAAAAAGGAAAACTAAACAATGGAAGCGGATGGTTACAAAAGCTACATTTGTTGGACCTGCTTTCACAAGGAAGCCTCCAAAGTACGAGCGTTTCATCCGTCCATCTGGTCTTCGTTTCACCAAGGCCCACGTCACTCACCCTGAACTAAAATGCACTTTCTGTCTCGAGATCATCGGGATTAAGAAGAATCCTAACGGTCCCATGTATACATCACTCGGTGTGATGACCAGGGGAACAATCATCGAGGTCAATGTGAGTGAGCTTGGTCTTGTTACACCAGCTGGAAAGGTTGTCTGGGGAAAATACGCTCAAGTGACAAACAATCCAGAGAACGACGGATGTATAAATGCTGTTTTACTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ribosomal_S8e

Protein Analysis

260

Amino Acids

29.75

Weight (kDa)

10.16

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 35 - 259 1.8e-37 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 481
AccI GTMKAC 1 cut(s) 623
AccII CGCG 1 cut(s) 88
AciI CCGC 1 cut(s) 442
AclWI GGATC 1 cut(s) 400
AcuI CTGAAG 1 cut(s) 405
AdeI CACNNNGTG 1 cut(s) 635
AfaI GTAC 1 cut(s) 500
AgsI TTSAA 1 cut(s) 196
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 1 cut(s) 644
AluBI AGCT 6 cut(s) 176, 254, 341, 458, 679, 698
AluI AGCT 6 cut(s) 176, 254, 341, 458, 679, 698
Alw26I GTCTC 2 cut(s) 129, 584
AlwI GGATC 1 cut(s) 400
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 581
AoxI GGCC 1 cut(s) 540
ArsI GACNNNNNNTTYG 2 cut(s) 339, 371
AspLEI GCGC 2 cut(s) 75, 88
AspS9I GGNCC 5 cut(s) 355, 370, 470, 541, 614
AsuHPI GGTGA 2 cut(s) 526, 545
AvaI CYCGRG 1 cut(s) 581
AvaII GGWCC 4 cut(s) 355, 370, 470, 614
AxyI CCTNAGG 1 cut(s) 6
BauI CACGAG 1 cut(s) 291
BbsI GAAGAC 2 cut(s) 288, 518
BccI CCATC 2 cut(s) 439, 526
BciT130I CCWGG 1 cut(s) 646
BcoDI GTCTC 2 cut(s) 129, 584
BfaI CTAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 481
Bme1390I CCNGG 1 cut(s) 646
Bme18I GGWCC 4 cut(s) 355, 370, 470, 614
BmeT110I CYCGRG 1 cut(s) 581
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 5 cut(s) 355, 370, 470, 541, 614
BmiI GGNNCC 2 cut(s) 357, 616
BmrFI CCNGG 1 cut(s) 646
BpiI GAAGAC 2 cut(s) 288, 518
BpuEI CTTGAG 1 cut(s) 711
BsaJI CCNNGG 4 cut(s) 111, 297, 537, 645
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bse1I ACTGG 1 cut(s) 377
Bse21I CCTNAGG 1 cut(s) 6
BseBI CCWGG 1 cut(s) 646
BseDI CCNNGG 4 cut(s) 111, 297, 537, 645
BseGI GGATG 3 cut(s) 450, 510, 761
BseMII CTCAG 2 cut(s) 20, 159
BseNI ACTGG 1 cut(s) 377
Bsh1236I CGCG 1 cut(s) 88
BshFI GGCC 1 cut(s) 542
BsiHKCI CYCGRG 1 cut(s) 581
BslFI GGGAC 2 cut(s) 341, 600
BsmAI GTCTC 2 cut(s) 129, 584
BsmFI GGGAC 2 cut(s) 341, 600
BsnI GGCC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 581
Bsp143I GATC 4 cut(s) 274, 405, 410, 585
Bsp19I CCATGG 1 cut(s) 111
BspACI CCGC 1 cut(s) 442
BspANI GGCC 1 cut(s) 542
BspCNI CTCAG 2 cut(s) 19, 160
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 1 cut(s) 180
BspLI GGNNCC 2 cut(s) 357, 616
BspMI ACCTGC 1 cut(s) 481
BspPI GGATC 1 cut(s) 400
BsrI ACTGG 1 cut(s) 377
BssECI CCNNGG 4 cut(s) 111, 297, 537, 645
BssMI GATC 4 cut(s) 274, 405, 410, 585
BssNAI GTATAC 1 cut(s) 624
BssSI CACGAG 1 cut(s) 291
BssT1I CCWWGG 3 cut(s) 111, 297, 537
Bst1107I GTATAC 1 cut(s) 624
Bst2BI CACGAG 1 cut(s) 291
Bst2UI CCWGG 1 cut(s) 646
Bst4CI ACNGT 1 cut(s) 614
Bst6I CTCTTC 3 cut(s) 29, 201, 366
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 4 cut(s) 6, 153, 168, 305
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 3 cut(s) 450, 510, 761
BstFNI CGCG 1 cut(s) 88
BstHHI GCGC 2 cut(s) 75, 88
BstKTI GATC 4 cut(s) 277, 408, 413, 588
BstMAI GTCTC 2 cut(s) 129, 584
BstMBI GATC 4 cut(s) 274, 405, 410, 585
BstMWI GCNNNNNNNGC 2 cut(s) 173, 260
BstNI CCWGG 1 cut(s) 646
BstSCI CCNGG 1 cut(s) 644
BstUI CGCG 1 cut(s) 88
BstV2I GAAGAC 2 cut(s) 288, 518
BstZ17I GTATAC 1 cut(s) 624
Bsu36I CCTNAGG 1 cut(s) 6
BsuRI GGCC 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 111
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 450, 510, 761
BveI ACCTGC 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 180
CfoI GCGC 2 cut(s) 75, 88
Cfr13I GGNCC 5 cut(s) 355, 370, 470, 541, 614
Csp6I GTAC 1 cut(s) 499
CviAII CATG 4 cut(s) 112, 181, 203, 619
CviQI GTAC 1 cut(s) 499
DdeI CTNAG 4 cut(s) 6, 153, 168, 305
DpnI GATC 4 cut(s) 276, 407, 412, 587
DpnII GATC 4 cut(s) 274, 405, 410, 585
DraIII CACNNNGTG 1 cut(s) 635
Eam1104I CTCTTC 3 cut(s) 29, 201, 366
EarI CTCTTC 3 cut(s) 29, 201, 366
Eco130I CCWWGG 3 cut(s) 111, 297, 537
Eco32I GATATC 1 cut(s) 47
Eco47I GGWCC 4 cut(s) 355, 370, 470, 614
Eco57I CTGAAG 1 cut(s) 405
Eco81I CCTNAGG 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 355
EcoRII CCWGG 1 cut(s) 644
EcoRV GATATC 1 cut(s) 47
EcoT14I CCWWGG 3 cut(s) 111, 297, 537
EcoT22I ATGCAT 1 cut(s) 204
ErhI CCWWGG 3 cut(s) 111, 297, 537
FaeI CATG 4 cut(s) 115, 184, 206, 622
FaiI YATR 8 cut(s) 20, 113, 165, 182, 204, 620, 624, 761
FaqI GGGAC 2 cut(s) 341, 600
FatI CATG 4 cut(s) 111, 180, 202, 618
FblI GTMKAC 1 cut(s) 623
FokI GGATG 3 cut(s) 457, 497, 768
FspBI CTAG 1 cut(s) 128
GlaI GCGC 2 cut(s) 74, 87
HaeIII GGCC 1 cut(s) 542
HhaI GCGC 2 cut(s) 75, 88
Hin1II CATG 4 cut(s) 115, 184, 206, 622
Hin6I GCGC 2 cut(s) 73, 86
HinP1I GCGC 2 cut(s) 73, 86
HinfI GANTC 2 cut(s) 209, 604
HphI GGTGA 2 cut(s) 526, 545
Hpy166II GTNNAC 2 cut(s) 97, 624
Hpy188I TCNGA 1 cut(s) 410
Hpy8I GTNNAC 2 cut(s) 97, 624
Hpy99I CGWCG 1 cut(s) 755
HpyAV CCTTC 2 cut(s) 73, 242
HpyCH4III ACNGT 1 cut(s) 614
HpyCH4IV ACGT 1 cut(s) 546
HpyCH4V TGCA 3 cut(s) 28, 202, 570
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 260
HpyF3I CTNAG 4 cut(s) 6, 153, 168, 305
HpySE526I ACGT 1 cut(s) 546
Hsp92II CATG 4 cut(s) 115, 184, 206, 622
HspAI GCGC 2 cut(s) 73, 86
Kzo9I GATC 4 cut(s) 274, 405, 410, 585
LmnI GCTCC 1 cut(s) 251
MaeI CTAG 1 cut(s) 128
MaeII ACGT 1 cut(s) 546
MaeIII GTNAC 4 cut(s) 448, 547, 688, 730
MalI GATC 4 cut(s) 276, 407, 412, 587
MboI GATC 4 cut(s) 274, 405, 410, 585
MboII GAAGA 9 cut(s) 46, 196, 218, 260, 293, 353, 398, 518, 613
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 1 cut(s) 448
MnlI CCTC 9 cut(s) 15, 30, 62, 76, 321, 346, 369, 501, 655
Mph1103I ATGCAT 1 cut(s) 204
MseI TTAA 2 cut(s) 318, 597
MspA1I CMGCKG 1 cut(s) 698
MspR9I CCNGG 1 cut(s) 646
MvaI CCWGG 1 cut(s) 646
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 2 cut(s) 173, 260
NcoI CCATGG 1 cut(s) 111
NdeII GATC 4 cut(s) 274, 405, 410, 585
NlaIII CATG 4 cut(s) 115, 184, 206, 622
NlaIV GGNNCC 2 cut(s) 357, 616
NmuCI GTSAC 2 cut(s) 547, 730
NsiI ATGCAT 1 cut(s) 204
PaeR7I CTCGAG 1 cut(s) 581
PagI TCATGA 1 cut(s) 180
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 1 cut(s) 604
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 1 cut(s) 644
PspGI CCWGG 1 cut(s) 644
PspN4I GGNNCC 2 cut(s) 357, 616
PspPI GGNCC 5 cut(s) 355, 370, 470, 541, 614
PspPPI RGGWCCY 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 383
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 1 cut(s) 500
RsaNI GTAC 1 cut(s) 499
SaqAI TTAA 2 cut(s) 318, 597
Sau3AI GATC 4 cut(s) 274, 405, 410, 585
Sau96I GGNCC 5 cut(s) 355, 370, 470, 541, 614
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 646
Sfr274I CTCGAG 1 cut(s) 581
SinI GGWCC 4 cut(s) 355, 370, 470, 614
SlaI CTCGAG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 581, 726
SmoI CTYRAG 2 cut(s) 581, 726
SsiI CCGC 1 cut(s) 442
SspMI CTAG 1 cut(s) 128
StyD4I CCNGG 1 cut(s) 644
StyI CCWWGG 3 cut(s) 111, 297, 537
TaaI ACNGT 1 cut(s) 614
TaiI ACGT 1 cut(s) 549
TaqI TCGA 4 cut(s) 54, 63, 582, 660
TfiI GAWTC 1 cut(s) 604
Tru1I TTAA 2 cut(s) 318, 597
Tru9I TTAA 2 cut(s) 318, 597
TseFI GTSAC 2 cut(s) 547, 730
Tsp45I GTSAC 2 cut(s) 547, 730
TspDTI ATGAA 4 cut(s) 197, 219, 405, 499
TspGWI ACGGA 3 cut(s) 362, 503, 768
VpaK11BI GGWCC 4 cut(s) 355, 370, 470, 614
XhoI CTCGAG 1 cut(s) 581
XmiI GTMKAC 1 cut(s) 623
XspI CTAG 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.