RchiOBHm_Chr4g0409741

Ribosome biogenesis protein NSA2 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002786
Physical Location & Seq
Forward (+)
33379043 .. 33381542
2500 bp
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UTR
Exon/CDS
Intron
PRQ38072

Sequence Viewer

Length: 783 bp
ATGCCGCAGGGAGATTATATAGATCTTCACAGGAAGCGATATGGGTACAGACCTGACCACTTTGAGCGCAAGCGCAAGAAGGAGGCTCGTGAGGTCCACAAGCGCTCTGAAATCGCGCAAAAGGCTCTGGGTATTAAAGGCAAGATGTTTGCCAAGAAACGCTATGCAGAAAAGGCTCTAATGAAGAAAACATTGGCTATGCATGAGGAGTCATCGACTAGGCACAAGGTTGATGATGATGTTCATGAAGGAGCTGTTCCAACATATTTGTTGGATCGTGACAATACAACACGAGCAAAGACTCTTAGCAATTCCATCAAGCAAAAACGGAAAGAGAAAGCTGGGAAATGGGAAGTCCCTCTGCCTAAGGTAAGGCCTGTGGCTGAAGATGAGATGTTTAAAGTGCTTCGAACGGGCAAACGCAAGACTAAGCAATGGAAGCGGATGATCACAAAAGCCACATTTGTGGGACCTGGTTTTACCAGGAAACCTCCCAAATATGAGCGATTTATCCGGCCCAGTGGATTGCGATTCACTAAAGCTCATGTGACACACCCTGAACTCAAATGTACTTTTAACCTTGAGATTATCGGTGTGAAGAAAAACCCCAATGGTCAAATGTACACCTCTCTTGGTGTCTTGACCAAGGGAACCATCATTGAGGTGAATGTAAGTGAACTTGGTCTTGTCACACCTAACGGGAAAGTTGTATGGGGGAAATATGCTCAGGTGACAAATAATCCAGAGAACGATGGATGTGTAAATGCAGTTCTGCTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S8e

Protein Analysis

260

Amino Acids

29.84

Weight (kDa)

10.11

Isoelectric Point (pI)

37.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8e PF01201 35 - 259 3.7e-41 Ribosomal protein S8e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 116
AciI CCGC 2 cut(s) 5, 442
AclWI GGATC 1 cut(s) 282
AcuI CTGAAG 1 cut(s) 405
AfaI GTAC 3 cut(s) 47, 571, 623
AfeI AGCGCT 1 cut(s) 104
AjnI CCWGG 2 cut(s) 472, 482
AjuI GAANNNNNNNTTGG 2 cut(s) 176, 208
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 3 cut(s) 254, 341, 542
AluI AGCT 3 cut(s) 254, 341, 542
AlwI GGATC 1 cut(s) 282
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 2 cut(s) 374, 515
AspLEI GCGC 4 cut(s) 69, 75, 105, 118
AspS9I GGNCC 3 cut(s) 94, 470, 516
AsuHPI GGTGA 2 cut(s) 676, 742
AsuII TTCGAA 1 cut(s) 409
AvaII GGWCC 2 cut(s) 94, 470
AxyI CCTNAGG 1 cut(s) 366
BauI CACGAG 2 cut(s) 87, 291
BccI CCATC 3 cut(s) 323, 662, 746
BciT130I CCWGG 2 cut(s) 474, 484
BclI TGATCA 1 cut(s) 447
BfaI CTAG 1 cut(s) 219
BfoI RGCGCY 1 cut(s) 106
BglII AGATCT 1 cut(s) 22
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 2 cut(s) 474, 484
Bme18I GGWCC 2 cut(s) 94, 470
BmgT120I GGNCC 3 cut(s) 94, 470, 516
BmiI GGNNCC 2 cut(s) 471, 652
BmrFI CCNGG 2 cut(s) 474, 484
BmrI ACTGGG 1 cut(s) 513
BmuI ACTGGG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 726
Bpu14I TTCGAA 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 645
Bse1I ACTGG 1 cut(s) 519
Bse21I CCTNAGG 1 cut(s) 366
Bse3DI GCAATG 1 cut(s) 440
BseBI CCWGG 2 cut(s) 474, 484
BseDI CCNNGG 1 cut(s) 645
BseGI GGATG 2 cut(s) 450, 761
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 1 cut(s) 519
BseRI GAGGAG 1 cut(s) 221
BseYI CCCAGC 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 2 cut(s) 376, 517
BsiSI CCGG 1 cut(s) 514
BslFI GGGAC 2 cut(s) 341, 483
BsmFI GGGAC 2 cut(s) 341, 483
BsnI GGCC 2 cut(s) 376, 517
Bsp119I TTCGAA 1 cut(s) 409
Bsp1407I TGTACA 1 cut(s) 621
Bsp143I GATC 3 cut(s) 22, 274, 447
BspACI CCGC 2 cut(s) 5, 442
BspANI GGCC 2 cut(s) 376, 517
BspCNI CTCAG 1 cut(s) 739
BspFNI CGCG 1 cut(s) 116
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 2 cut(s) 471, 652
BspPI GGATC 1 cut(s) 282
BspT104I TTCGAA 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 621
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 645
BssMI GATC 3 cut(s) 22, 274, 447
BssSI CACGAG 2 cut(s) 87, 291
BssT1I CCWWGG 1 cut(s) 645
Bst2BI CACGAG 2 cut(s) 87, 291
Bst2UI CCWGG 2 cut(s) 474, 484
BstAUI TGTACA 1 cut(s) 621
BstBI TTCGAA 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 4 cut(s) 305, 366, 429, 726
BstF5I GGATG 2 cut(s) 450, 761
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 1 cut(s) 106
BstHHI GCGC 4 cut(s) 69, 75, 105, 118
BstKTI GATC 3 cut(s) 25, 277, 450
BstMBI GATC 3 cut(s) 22, 274, 447
BstMWI GCNNNNNNNGC 3 cut(s) 122, 173, 439
BstNI CCWGG 2 cut(s) 474, 484
BstSCI CCNGG 2 cut(s) 472, 482
BstUI CGCG 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 466
BstYI RGATCY 1 cut(s) 22
Bsu36I CCTNAGG 1 cut(s) 366
BsuRI GGCC 2 cut(s) 376, 517
BtsCI GGATG 2 cut(s) 450, 761
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 71
CciI TCATGA 1 cut(s) 244
CfoI GCGC 4 cut(s) 69, 75, 105, 118
Cfr13I GGNCC 3 cut(s) 94, 470, 516
CsiI ACCWGGT 1 cut(s) 472
Csp6I GTAC 3 cut(s) 46, 570, 622
CviAII CATG 3 cut(s) 203, 245, 545
CviQI GTAC 3 cut(s) 46, 570, 622
DdeI CTNAG 4 cut(s) 305, 366, 429, 726
DpnI GATC 3 cut(s) 24, 276, 449
DpnII GATC 3 cut(s) 22, 274, 447
DraI TTTAAA 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 645
Eco147I AGGCCT 1 cut(s) 376
Eco47I GGWCC 2 cut(s) 94, 470
Eco47III AGCGCT 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 405
Eco81I CCTNAGG 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 470
EcoRII CCWGG 2 cut(s) 472, 482
EcoT14I CCWWGG 1 cut(s) 645
EcoT22I ATGCAT 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 3 cut(s) 206, 248, 548
FaqI GGGAC 2 cut(s) 341, 483
FatI CATG 3 cut(s) 202, 244, 544
FbaI TGATCA 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 457, 768
Fsp4HI GCNGC 1 cut(s) 5
FspBI CTAG 1 cut(s) 219
GlaI GCGC 4 cut(s) 68, 74, 104, 117
GluI GCNGC 1 cut(s) 5
GsaI CCCAGC 1 cut(s) 345
HaeII RGCGCY 1 cut(s) 106
HaeIII GGCC 2 cut(s) 376, 517
HapII CCGG 1 cut(s) 514
HhaI GCGC 4 cut(s) 69, 75, 105, 118
Hin1II CATG 3 cut(s) 206, 248, 548
Hin6I GCGC 4 cut(s) 67, 73, 103, 116
HinP1I GCGC 4 cut(s) 67, 73, 103, 116
HinfI GANTC 3 cut(s) 209, 301, 531
HpaII CCGG 1 cut(s) 514
HphI GGTGA 2 cut(s) 676, 742
Hpy166II GTNNAC 3 cut(s) 97, 624, 677
Hpy188I TCNGA 1 cut(s) 109
Hpy188III TCNNGA 5 cut(s) 89, 245, 278, 640, 743
Hpy8I GTNNAC 3 cut(s) 97, 624, 677
HpyAV CCTTC 2 cut(s) 73, 242
HpyCH4V TGCA 3 cut(s) 167, 202, 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 122, 173, 439
HpyF3I CTNAG 4 cut(s) 305, 366, 429, 726
Hsp92II CATG 3 cut(s) 206, 248, 548
HspAI GCGC 4 cut(s) 67, 73, 103, 116
Ksp22I TGATCA 1 cut(s) 447
Kzo9I GATC 3 cut(s) 22, 274, 447
LmnI GCTCC 1 cut(s) 251
MabI ACCWGGT 1 cut(s) 472
MaeI CTAG 1 cut(s) 219
MaeIII GTNAC 4 cut(s) 278, 547, 688, 730
MalI GATC 3 cut(s) 24, 276, 449
MboI GATC 3 cut(s) 22, 274, 447
MboII GAAGA 4 cut(s) 17, 196, 398, 610
MflI RGATCY 1 cut(s) 22
MluCI AATT 1 cut(s) 310
MlyI GAGTC 2 cut(s) 218, 295
MmeI TCCRAC 2 cut(s) 252, 284
MnlI CCTC 7 cut(s) 76, 85, 199, 369, 501, 637, 655
Mph1103I ATGCAT 1 cut(s) 204
MseI TTAA 3 cut(s) 135, 399, 576
MslI CAYNNNNRTG 2 cut(s) 464, 662
MspI CCGG 1 cut(s) 514
MspR9I CCNGG 2 cut(s) 474, 484
MvaI CCWGG 2 cut(s) 474, 484
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 3 cut(s) 122, 173, 439
NdeII GATC 3 cut(s) 22, 274, 447
NlaIII CATG 3 cut(s) 206, 248, 548
NlaIV GGNNCC 2 cut(s) 471, 652
NmuCI GTSAC 4 cut(s) 278, 547, 688, 730
NsiI ATGCAT 1 cut(s) 204
NspV TTCGAA 1 cut(s) 409
OliI CACNNNNGTG 1 cut(s) 464
PagI TCATGA 1 cut(s) 244
PceI AGGCCT 1 cut(s) 376
PfeI GAWTC 1 cut(s) 531
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 2 cut(s) 217, 295
PpsI GAGTC 2 cut(s) 217, 295
PpuMI RGGWCCY 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 470
Psp6I CCWGG 2 cut(s) 472, 482
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 472, 482
PspN4I GGNNCC 2 cut(s) 471, 652
PspPI GGNCC 3 cut(s) 94, 470, 516
PspPPI RGGWCCY 1 cut(s) 470
PsrI GAACNNNNNNTAC 1 cut(s) 753
PsuI RGATCY 1 cut(s) 22
RsaI GTAC 3 cut(s) 47, 571, 623
RsaNI GTAC 3 cut(s) 46, 570, 622
RseI CAYNNNNRTG 2 cut(s) 464, 662
SaqAI TTAA 3 cut(s) 135, 399, 576
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 3 cut(s) 22, 274, 447
Sau96I GGNCC 3 cut(s) 94, 470, 516
SchI GAGTC 2 cut(s) 218, 295
ScrFI CCNGG 2 cut(s) 474, 484
SexAI ACCWGGT 1 cut(s) 472
SfuI TTCGAA 1 cut(s) 409
SinI GGWCC 2 cut(s) 94, 470
SmiMI CAYNNNNRTG 2 cut(s) 464, 662
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 1 cut(s) 310
SseBI AGGCCT 1 cut(s) 376
SsiI CCGC 2 cut(s) 5, 442
SspMI CTAG 1 cut(s) 219
StuI AGGCCT 1 cut(s) 376
StyD4I CCNGG 2 cut(s) 472, 482
StyI CCWWGG 1 cut(s) 645
TaqI TCGA 2 cut(s) 215, 409
TasI AATT 1 cut(s) 310
TatI WGTACW 2 cut(s) 569, 621
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 3 cut(s) 135, 399, 576
Tru9I TTAA 3 cut(s) 135, 399, 576
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 4 cut(s) 278, 547, 688, 730
Tsp45I GTSAC 4 cut(s) 278, 547, 688, 730
TspDTI ATGAA 3 cut(s) 197, 233, 261
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 1 cut(s) 526
VpaK11BI GGWCC 2 cut(s) 94, 470
XspI CTAG 1 cut(s) 219
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.