Belongs to the GDA1 CD39 NTPase family
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| Species | Orthologous Gene IDs |
|---|---|
| malus_domestica | MD11G1180600.v1.1 |
| prunus_persica | Prupe.6G153100_v2.0.a1 |
| pyrus_communis | pycom11g15580 |
| rosa_chinensis | RchiOBHm_Chr5g0049751 |
| rosa_laevigata | RLG00000034644 |
| rosa_multiflora | Rmu_sc0016686.1_g000002 Rmu_sc0020783.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00031680 |
| rosa_rugosa | Rorug05G0250800 Rorug05G0250800 Rorug05G0250900 |
| rosa_samantha | Rh5AG326900 Rh5BG338000 Rh5CG362600 Rh5DG350200 |
| rosa_wichuraiana | Rw0G018430 Rw5G030880 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 376 |
| Acc36I | ACCTGC | 3 cut(s) | 376, 652, 1127 |
| AccB1I | GGYRCC | 2 cut(s) | 275, 388 |
| AccII | CGCG | 4 cut(s) | 230, 232, 281, 342 |
| AclI | AACGTT | 1 cut(s) | 1144 |
| AcoI | YGGCCR | 1 cut(s) | 772 |
| AcsI | RAATTY | 3 cut(s) | 185, 697, 838 |
| AcuI | CTGAAG | 1 cut(s) | 981 |
| AcyI | GRCGYC | 1 cut(s) | 216 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 56, 442, 770 |
| AgsI | TTSAA | 3 cut(s) | 523, 641, 1213 |
| AjnI | CCWGG | 2 cut(s) | 547, 774 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 407, 439 |
| Alw26I | GTCTC | 4 cut(s) | 34, 229, 714, 1027 |
| Ama87I | CYCGRG | 2 cut(s) | 437, 537 |
| AoxI | GGCC | 5 cut(s) | 310, 433, 455, 534, 772 |
| ApaI | GGGCCC | 1 cut(s) | 538 |
| ApeKI | GCWGC | 6 cut(s) | 60, 382, 674, 782, 805, 1133 |
| ApoI | RAATTY | 3 cut(s) | 185, 697, 838 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 817, 849 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 943 |
| AspLEI | GCGC | 4 cut(s) | 232, 234, 283, 344 |
| AspS9I | GGNCC | 5 cut(s) | 351, 434, 455, 534, 535 |
| AsuC2I | CCSGG | 2 cut(s) | 538, 539 |
| AsuHPI | GGTGA | 3 cut(s) | 118, 563, 589 |
| AsuII | TTCGAA | 1 cut(s) | 836 |
| AvaI | CYCGRG | 2 cut(s) | 437, 537 |
| AvaII | GGWCC | 1 cut(s) | 351 |
| AxyI | CCTNAGG | 2 cut(s) | 459, 618 |
| BaeGI | GKGCMC | 2 cut(s) | 393, 538 |
| BalI | TGGCCA | 1 cut(s) | 774 |
| BanI | GGYRCC | 2 cut(s) | 275, 388 |
| BanII | GRGCYC | 1 cut(s) | 538 |
| BbvCI | CCTCAGC | 1 cut(s) | 500 |
| BbvI | GCAGC | 6 cut(s) | 72, 369, 661, 792, 794, 1145 |
| BccI | CCATC | 1 cut(s) | 266 |
| BceAI | ACGGC | 2 cut(s) | 227, 1125 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 428, 462 |
| BciT130I | CCWGG | 2 cut(s) | 549, 776 |
| BcnI | CCSGG | 2 cut(s) | 538, 539 |
| BcoDI | GTCTC | 4 cut(s) | 34, 229, 714, 1027 |
| BfaI | CTAG | 4 cut(s) | 689, 816, 876, 1262 |
| BfmI | CTRYAG | 2 cut(s) | 383, 806 |
| BfuAI | ACCTGC | 3 cut(s) | 376, 652, 1127 |
| BisI | GCNGC | 9 cut(s) | 61, 240, 327, 383, 675, 764, 783, 806, 1134 |
| BlsI | GCNGC | 9 cut(s) | 62, 241, 328, 384, 676, 765, 784, 807, 1135 |
| Bme1390I | CCNGG | 4 cut(s) | 538, 539, 549, 776 |
| Bme18I | GGWCC | 1 cut(s) | 351 |
| BmeT110I | CYCGRG | 2 cut(s) | 437, 537 |
| BmgT120I | GGNCC | 5 cut(s) | 351, 434, 455, 534, 535 |
| BmiI | GGNNCC | 8 cut(s) | 277, 352, 353, 390, 436, 456, 536, 1104 |
| BmrFI | CCNGG | 4 cut(s) | 538, 539, 549, 776 |
| BmrI | ACTGGG | 2 cut(s) | 48, 639 |
| BmsI | GCATC | 4 cut(s) | 264, 436, 769, 1200 |
| BmuI | ACTGGG | 2 cut(s) | 48, 639 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 601, 633 |
| BpmI | CTGGAG | 1 cut(s) | 271 |
| Bpu10I | CCTNAGC | 1 cut(s) | 500 |
| Bpu14I | TTCGAA | 1 cut(s) | 836 |
| BpuMI | CCSGG | 2 cut(s) | 538, 539 |
| BsaHI | GRCGYC | 1 cut(s) | 216 |
| BsaI | GGTCTC | 1 cut(s) | 34 |
| BsaJI | CCNNGG | 4 cut(s) | 307, 537, 1107, 1272 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 137, 167 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 56, 442, 770 |
| Bse118I | RCCGGY | 1 cut(s) | 431 |
| Bse1I | ACTGG | 4 cut(s) | 54, 634, 1055, 1311 |
| Bse21I | CCTNAGG | 2 cut(s) | 459, 618 |
| Bse3DI | GCAATG | 1 cut(s) | 602 |
| BseBI | CCWGG | 2 cut(s) | 549, 776 |
| BseDI | CCNNGG | 4 cut(s) | 307, 537, 1107, 1272 |
| BseGI | GGATG | 6 cut(s) | 105, 451, 784, 1025, 1282, 1313 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 56, 442, 770 |
| BseMI | GCAATG | 1 cut(s) | 602 |
| BseMII | CTCAG | 6 cut(s) | 450, 459, 491, 632, 1077, 1215 |
| BseNI | ACTGG | 4 cut(s) | 54, 634, 1055, 1311 |
| BsePI | GCGCGC | 1 cut(s) | 230 |
| BseRI | GAGGAG | 7 cut(s) | 55, 58, 113, 140, 289, 356, 410 |
| BseSI | GKGCMC | 2 cut(s) | 393, 538 |
| BseXI | GCAGC | 6 cut(s) | 72, 369, 661, 792, 794, 1145 |
| Bsh1236I | CGCG | 4 cut(s) | 230, 232, 281, 342 |
| Bsh1285I | CGRYCG | 1 cut(s) | 340 |
| BshFI | GGCC | 5 cut(s) | 312, 435, 457, 536, 774 |
| BshNI | GGYRCC | 2 cut(s) | 275, 388 |
| BsiEI | CGRYCG | 1 cut(s) | 340 |
| BsiHKCI | CYCGRG | 2 cut(s) | 437, 537 |
| BsiSI | CCGG | 2 cut(s) | 432, 538 |
| BslFI | GGGAC | 3 cut(s) | 337, 344, 664 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 56, 442, 770 |
| BsmAI | GTCTC | 4 cut(s) | 34, 229, 714, 1027 |
| BsmBI | CGTCTC | 2 cut(s) | 229, 714 |
| BsmFI | GGGAC | 3 cut(s) | 337, 344, 664 |
| BsmI | GAATGC | 1 cut(s) | 904 |
| BsnI | GGCC | 5 cut(s) | 312, 435, 457, 536, 774 |
| Bso31I | GGTCTC | 1 cut(s) | 34 |
| BsoBI | CYCGRG | 2 cut(s) | 437, 537 |
| Bsp119I | TTCGAA | 1 cut(s) | 836 |
| Bsp120I | GGGCCC | 1 cut(s) | 534 |
| Bsp1286I | GDGCHC | 2 cut(s) | 393, 538 |
| Bsp143I | GATC | 1 cut(s) | 337 |
| Bsp19I | CCATGG | 2 cut(s) | 307, 1272 |
| BspANI | GGCC | 5 cut(s) | 312, 435, 457, 536, 774 |
| BspCNI | CTCAG | 6 cut(s) | 451, 460, 492, 631, 1078, 1214 |
| BspFNI | CGCG | 4 cut(s) | 230, 232, 281, 342 |
| BspLI | GGNNCC | 8 cut(s) | 277, 352, 353, 390, 436, 456, 536, 1104 |
| BspMAI | CTGCAG | 2 cut(s) | 387, 810 |
| BspMI | ACCTGC | 3 cut(s) | 376, 652, 1127 |
| BspT104I | TTCGAA | 1 cut(s) | 836 |
| BspT107I | GGYRCC | 2 cut(s) | 275, 388 |
| BspTNI | GGTCTC | 1 cut(s) | 34 |
| BsrDI | GCAATG | 1 cut(s) | 602 |
| BsrFI | RCCGGY | 1 cut(s) | 431 |
| BsrI | ACTGG | 4 cut(s) | 54, 634, 1055, 1311 |
| BssAI | RCCGGY | 1 cut(s) | 431 |
| BssECI | CCNNGG | 4 cut(s) | 307, 537, 1107, 1272 |
| BssHII | GCGCGC | 1 cut(s) | 230 |
| BssMI | GATC | 1 cut(s) | 337 |
| BssNI | GRCGYC | 1 cut(s) | 216 |
| BssT1I | CCWWGG | 2 cut(s) | 307, 1272 |
| Bst2UI | CCWGG | 2 cut(s) | 549, 776 |
| Bst4CI | ACNGT | 6 cut(s) | 752, 798, 895, 1151, 1248, 1259 |
| Bst6I | CTCTTC | 3 cut(s) | 146, 215, 428 |
| BstACI | GRCGYC | 1 cut(s) | 216 |
| BstBI | TTCGAA | 1 cut(s) | 836 |
| BstC8I | GCNNGC | 6 cut(s) | 232, 346, 433, 761, 1001, 1005 |
| BstDEI | CTNAG | 7 cut(s) | 459, 468, 500, 618, 843, 1086, 1201 |
| BstDSI | CCRYGG | 3 cut(s) | 307, 1107, 1272 |
| BstEII | GGTNACC | 1 cut(s) | 860 |
| BstF5I | GGATG | 6 cut(s) | 105, 451, 784, 1025, 1282, 1313 |
| BstFNI | CGCG | 4 cut(s) | 230, 232, 281, 342 |
| BstHHI | GCGC | 4 cut(s) | 232, 234, 283, 344 |
| BstKTI | GATC | 1 cut(s) | 340 |
| BstMAI | GTCTC | 4 cut(s) | 34, 229, 714, 1027 |
| BstMBI | GATC | 1 cut(s) | 337 |
| BstMCI | CGRYCG | 1 cut(s) | 340 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 236, 326, 388, 613, 1096 |
| BstNI | CCWGG | 2 cut(s) | 549, 776 |
| BstNSI | RCATGY | 1 cut(s) | 1003 |
| BstPI | GGTNACC | 1 cut(s) | 860 |
| BstSCI | CCNGG | 4 cut(s) | 536, 537, 547, 774 |
| BstSFI | CTRYAG | 2 cut(s) | 383, 806 |
| BstSLI | GKGCMC | 2 cut(s) | 393, 538 |
| BstUI | CGCG | 4 cut(s) | 230, 232, 281, 342 |
| BstV1I | GCAGC | 6 cut(s) | 72, 369, 661, 792, 794, 1145 |
| Bsu36I | CCTNAGG | 2 cut(s) | 459, 618 |
| BsuRI | GGCC | 5 cut(s) | 312, 435, 457, 536, 774 |
| BtgI | CCRYGG | 3 cut(s) | 307, 1107, 1272 |
| BtsCI | GGATG | 6 cut(s) | 105, 451, 784, 1025, 1282, 1313 |
| BtsIMutI | CAGTG | 4 cut(s) | 61, 794, 900, 1244 |
| BveI | ACCTGC | 3 cut(s) | 376, 652, 1127 |
| Cac8I | GCNNGC | 6 cut(s) | 232, 346, 433, 761, 1001, 1005 |
| CfoI | GCGC | 4 cut(s) | 232, 234, 283, 344 |
| Cfr10I | RCCGGY | 1 cut(s) | 431 |
| Cfr13I | GGNCC | 5 cut(s) | 351, 434, 455, 534, 535 |
| Cfr9I | CCCGGG | 1 cut(s) | 537 |
| CseI | GACGC | 1 cut(s) | 205 |
| CviAII | CATG | 4 cut(s) | 308, 1000, 1253, 1273 |
| DdeI | CTNAG | 7 cut(s) | 459, 468, 500, 618, 843, 1086, 1201 |
| DpnI | GATC | 1 cut(s) | 339 |
| DpnII | GATC | 1 cut(s) | 337 |
| EaeI | YGGCCR | 1 cut(s) | 772 |
| Eam1104I | CTCTTC | 3 cut(s) | 146, 215, 428 |
| EarI | CTCTTC | 3 cut(s) | 146, 215, 428 |
| EciI | GGCGGA | 1 cut(s) | 236 |
| Eco130I | CCWWGG | 2 cut(s) | 307, 1272 |
| Eco24I | GRGCYC | 1 cut(s) | 538 |
| Eco31I | GGTCTC | 1 cut(s) | 34 |
| Eco47I | GGWCC | 1 cut(s) | 351 |
| Eco57I | CTGAAG | 1 cut(s) | 981 |
| Eco81I | CCTNAGG | 2 cut(s) | 459, 618 |
| Eco88I | CYCGRG | 2 cut(s) | 437, 537 |
| Eco91I | GGTNACC | 1 cut(s) | 860 |
| EcoO109I | RGGNCCY | 2 cut(s) | 351, 455 |
| EcoO65I | GGTNACC | 1 cut(s) | 860 |
| EcoRI | GAATTC | 2 cut(s) | 697, 838 |
| EcoRII | CCWGG | 2 cut(s) | 547, 774 |
| EcoT14I | CCWWGG | 2 cut(s) | 307, 1272 |
| EcoT22I | ATGCAT | 1 cut(s) | 1001 |
| EcoT38I | GRGCYC | 1 cut(s) | 538 |
| ErhI | CCWWGG | 2 cut(s) | 307, 1272 |
| Esp3I | CGTCTC | 2 cut(s) | 229, 714 |
| FaeI | CATG | 4 cut(s) | 311, 1003, 1256, 1276 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 407, 439, 1058, 1090 |
| FaqI | GGGAC | 3 cut(s) | 337, 344, 664 |
| FatI | CATG | 4 cut(s) | 307, 999, 1252, 1272 |
| FauI | CCCGC | 3 cut(s) | 80, 244, 341 |
| Fnu4HI | GCNGC | 9 cut(s) | 61, 240, 327, 383, 675, 764, 783, 806, 1134 |
| FokI | GGATG | 6 cut(s) | 92, 458, 791, 1012, 1289, 1300 |
| FriOI | GRGCYC | 1 cut(s) | 538 |
| Fsp4HI | GCNGC | 9 cut(s) | 61, 240, 327, 383, 675, 764, 783, 806, 1134 |
| FspBI | CTAG | 4 cut(s) | 689, 816, 876, 1262 |
| GlaI | GCGC | 4 cut(s) | 231, 233, 282, 343 |
| GluI | GCNGC | 9 cut(s) | 61, 240, 327, 383, 675, 764, 783, 806, 1134 |
| GsuI | CTGGAG | 1 cut(s) | 271 |
| HaeIII | GGCC | 5 cut(s) | 312, 435, 457, 536, 774 |
| HapII | CCGG | 2 cut(s) | 432, 538 |
| HgaI | GACGC | 1 cut(s) | 205 |
| HhaI | GCGC | 4 cut(s) | 232, 234, 283, 344 |
| Hin1I | GRCGYC | 1 cut(s) | 216 |
| Hin1II | CATG | 4 cut(s) | 311, 1003, 1256, 1276 |
| Hin6I | GCGC | 4 cut(s) | 230, 232, 281, 342 |
| HinP1I | GCGC | 4 cut(s) | 230, 232, 281, 342 |
| HincII | GTYRAC | 4 cut(s) | 12, 82, 1142, 1198 |
| HindII | GTYRAC | 4 cut(s) | 12, 82, 1142, 1198 |
| HindIII | AAGCTT | 2 cut(s) | 473, 963 |
| HinfI | GANTC | 6 cut(s) | 200, 482, 683, 791, 1199, 1266 |
| HpaII | CCGG | 2 cut(s) | 432, 538 |
| HphI | GGTGA | 3 cut(s) | 118, 563, 589 |
| Hpy166II | GTNNAC | 4 cut(s) | 12, 82, 1142, 1198 |
| Hpy188I | TCNGA | 4 cut(s) | 199, 961, 1087, 1204 |
| Hpy188III | TCNNGA | 6 cut(s) | 269, 479, 620, 711, 816, 1320 |
| Hpy8I | GTNNAC | 4 cut(s) | 12, 82, 1142, 1198 |
| Hpy99I | CGWCG | 3 cut(s) | 221, 224, 511 |
| HpyAV | CCTTC | 7 cut(s) | 98, 140, 145, 331, 391, 487, 978 |
| HpyCH4III | ACNGT | 6 cut(s) | 752, 798, 895, 1151, 1248, 1259 |
| HpyCH4IV | ACGT | 2 cut(s) | 78, 1144 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 236, 326, 388, 613, 1096 |
| HpyF3I | CTNAG | 7 cut(s) | 459, 468, 500, 618, 843, 1086, 1201 |
| HpySE526I | ACGT | 2 cut(s) | 78, 1144 |
| Hsp92I | GRCGYC | 1 cut(s) | 216 |
| Hsp92II | CATG | 4 cut(s) | 311, 1003, 1256, 1276 |
| HspAI | GCGC | 4 cut(s) | 230, 232, 281, 342 |
| KflI | GGGWCCC | 1 cut(s) | 351 |
| KroI | GCCGGC | 1 cut(s) | 431 |
| KroNI | GCCGGC | 1 cut(s) | 433 |
| Kzo9I | GATC | 1 cut(s) | 337 |
| LmnI | GCTCC | 5 cut(s) | 68, 174, 296, 400, 423 |
| Lsp1109I | GCAGC | 6 cut(s) | 72, 369, 661, 792, 794, 1145 |
| LweI | GCATC | 4 cut(s) | 264, 436, 769, 1200 |
| MaeI | CTAG | 4 cut(s) | 689, 816, 876, 1262 |
| MaeII | ACGT | 2 cut(s) | 78, 1144 |
| MaeIII | GTNAC | 5 cut(s) | 664, 733, 860, 1041, 1145 |
| MalI | GATC | 1 cut(s) | 339 |
| MboI | GATC | 1 cut(s) | 337 |
| MboII | GAAGA | 7 cut(s) | 133, 202, 250, 415, 693, 939, 1166 |
| MhlI | GDGCHC | 2 cut(s) | 393, 538 |
| MlsI | TGGCCA | 1 cut(s) | 774 |
| MluCI | AATT | 7 cut(s) | 185, 697, 714, 722, 821, 838, 1017 |
| MluNI | TGGCCA | 1 cut(s) | 774 |
| MlyI | GAGTC | 1 cut(s) | 1193 |
| MmeI | TCCRAC | 3 cut(s) | 43, 1107, 1270 |
| Mox20I | TGGCCA | 1 cut(s) | 774 |
| Mph1103I | ATGCAT | 1 cut(s) | 1001 |
| MroNI | GCCGGC | 1 cut(s) | 431 |
| MroXI | GAANNNNTTC | 1 cut(s) | 943 |
| MscI | TGGCCA | 1 cut(s) | 774 |
| MseI | TTAA | 1 cut(s) | 977 |
| Msp20I | TGGCCA | 1 cut(s) | 774 |
| MspI | CCGG | 2 cut(s) | 432, 538 |
| MspR9I | CCNGG | 4 cut(s) | 538, 539, 549, 776 |
| Mva1269I | GAATGC | 1 cut(s) | 904 |
| MvaI | CCWGG | 2 cut(s) | 549, 776 |
| MvnI | CGCG | 4 cut(s) | 230, 232, 281, 342 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 236, 326, 388, 613, 1096 |
| NaeI | GCCGGC | 1 cut(s) | 433 |
| NciI | CCSGG | 2 cut(s) | 538, 539 |
| NcoI | CCATGG | 2 cut(s) | 307, 1272 |
| NdeII | GATC | 1 cut(s) | 337 |
| NgoMIV | GCCGGC | 1 cut(s) | 431 |
| NlaIII | CATG | 4 cut(s) | 311, 1003, 1256, 1276 |
| NlaIV | GGNNCC | 8 cut(s) | 277, 352, 353, 390, 436, 456, 536, 1104 |
| NmuCI | GTSAC | 1 cut(s) | 664 |
| NsiI | ATGCAT | 1 cut(s) | 1001 |
| NspI | RCATGY | 1 cut(s) | 1003 |
| NspV | TTCGAA | 1 cut(s) | 836 |
| PaeI | GCATGC | 1 cut(s) | 1003 |
| PaqCI | CACCTGC | 1 cut(s) | 376 |
| PauI | GCGCGC | 1 cut(s) | 230 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 195, 219 |
| PctI | GAATGC | 1 cut(s) | 904 |
| PdiI | GCCGGC | 1 cut(s) | 433 |
| PdmI | GAANNNNTTC | 1 cut(s) | 943 |
| PfeI | GAWTC | 5 cut(s) | 200, 482, 683, 791, 1266 |
| PkrI | GCNGC | 9 cut(s) | 62, 241, 328, 384, 676, 765, 784, 807, 1135 |
| Ple19I | CGATCG | 1 cut(s) | 340 |
| PleI | GAGTC | 1 cut(s) | 1193 |
| PpsI | GAGTC | 1 cut(s) | 1193 |
| PpuMI | RGGWCCY | 1 cut(s) | 351 |
| Psp1406I | AACGTT | 1 cut(s) | 1144 |
| Psp5II | RGGWCCY | 1 cut(s) | 351 |
| Psp6I | CCWGG | 2 cut(s) | 547, 774 |
| PspEI | GGTNACC | 1 cut(s) | 860 |
| PspGI | CCWGG | 2 cut(s) | 547, 774 |
| PspN4I | GGNNCC | 8 cut(s) | 277, 352, 353, 390, 436, 456, 536, 1104 |
| PspOMI | GGGCCC | 1 cut(s) | 534 |
| PspPI | GGNCC | 5 cut(s) | 351, 434, 455, 534, 535 |
| PspPPI | RGGWCCY | 1 cut(s) | 351 |
| PstI | CTGCAG | 2 cut(s) | 387, 810 |
| PteI | GCGCGC | 1 cut(s) | 230 |
| PvuI | CGATCG | 1 cut(s) | 340 |
| SaqAI | TTAA | 1 cut(s) | 977 |
| SatI | GCNGC | 9 cut(s) | 61, 240, 327, 383, 675, 764, 783, 806, 1134 |
| Sau3AI | GATC | 1 cut(s) | 337 |
| Sau96I | GGNCC | 5 cut(s) | 351, 434, 455, 534, 535 |
| SchI | GAGTC | 1 cut(s) | 1193 |
| ScrFI | CCNGG | 4 cut(s) | 538, 539, 549, 776 |
| SduI | GDGCHC | 2 cut(s) | 393, 538 |
| SfaNI | GCATC | 4 cut(s) | 264, 436, 769, 1200 |
| SfcI | CTRYAG | 2 cut(s) | 383, 806 |
| SfuI | TTCGAA | 1 cut(s) | 836 |
| SinI | GGWCC | 1 cut(s) | 351 |
| SmaI | CCCGGG | 1 cut(s) | 539 |
| SphI | GCATGC | 1 cut(s) | 1003 |
| Sse9I | AATT | 7 cut(s) | 185, 697, 714, 722, 821, 838, 1017 |
| SspI | AATATT | 1 cut(s) | 1217 |
| SspMI | CTAG | 4 cut(s) | 689, 816, 876, 1262 |
| StyD4I | CCNGG | 4 cut(s) | 536, 537, 547, 774 |
| StyI | CCWWGG | 2 cut(s) | 307, 1272 |
| TaaI | ACNGT | 6 cut(s) | 752, 798, 895, 1151, 1248, 1259 |
| TaiI | ACGT | 2 cut(s) | 81, 1147 |
| TaqI | TCGA | 3 cut(s) | 270, 712, 836 |
| TasI | AATT | 7 cut(s) | 185, 697, 714, 722, 821, 838, 1017 |
| TauI | GCSGC | 3 cut(s) | 242, 329, 766 |
| TfiI | GAWTC | 5 cut(s) | 200, 482, 683, 791, 1266 |
| Tru1I | TTAA | 1 cut(s) | 977 |
| Tru9I | TTAA | 1 cut(s) | 977 |
| TscAI | CASTG | 4 cut(s) | 61, 801, 900, 1251 |
| TseFI | GTSAC | 1 cut(s) | 664 |
| TseI | GCWGC | 6 cut(s) | 60, 382, 674, 782, 805, 1133 |
| Tsp45I | GTSAC | 1 cut(s) | 664 |
| TspGWI | ACGGA | 1 cut(s) | 696 |
| TspMI | CCCGGG | 1 cut(s) | 537 |
| TspRI | CASTG | 4 cut(s) | 61, 801, 900, 1251 |
| VpaK11BI | GGWCC | 1 cut(s) | 351 |
| XapI | RAATTY | 3 cut(s) | 185, 697, 838 |
| XbaI | TCTAGA | 1 cut(s) | 815 |
| XceI | RCATGY | 1 cut(s) | 1003 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 269 |
| XmaI | CCCGGG | 1 cut(s) | 537 |
| XmnI | GAANNNNTTC | 1 cut(s) | 943 |
| XspI | CTAG | 4 cut(s) | 689, 816, 876, 1262 |
| Zsp2I | ATGCAT | 1 cut(s) | 1001 |