Rroxscaffold_1G00031680

Belongs to the GDA1 CD39 NTPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014823
Physical Location & Seq
Forward (+)
44925987 .. 44935576
9590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00031680.1

Sequence Viewer

Length: 1545 bp
ATGGATTTGTCAACCCTCCAAATCCAATCGAAGAGAGCTCCACCACCACCAGTCTCTTCTTCCTCCTCCTCCTCTCGCAACAATCGCCAATGCCTCCTCATCTCCTGCCTCTCTCTCCTCGCCCTCCCCTTCCTCTTCTACCTCGTCTCCACCGCCCACAAGCTCCACCTCTCCCCCAAATTCACCCACTCCGAATCCACCCTCTTCGGCATTGTCATCACCGCCGCCCCCACCGCCTCCCGCATCCGCGTCTTCCATTTCCTCGACGCCACCGCCACCGCCCCCTCCACCTCCTCCCGCTCTCTCCCCGCCCTCTCCTCCTTCGCCGCCCGCCCCGACGCCGCCGGCGCCTCCCTCTCGCCGCTGCTCCGCTTCGCCAAGTCGCAGGTGCCGATGAAAGAGCGAGCCAGTACGAAGCTGCTCTTGTTTGCTTCCCCGGAGCTCCAGGACTTGGGCCAGGAGATCGCCGAGAAGCTTCTCGAGTCGTGTAGGAAGGTGCTCAGATCTTCCGGGTTTTGGTTCAAGGACGATTGGGCCAGAGTGGTCTCAGGTGAAGAGCAAGGGGTTTATGCTTGGGTGAGTGCCAATTACGCTCTCGGAACATTGAAGAGTGAGGCTGAAGAAACCACTGGGATAGTTGAACTCGGTGGGGCTTCGTTGCAGGTTACATTTGCCCAAAAAGAAACACTCCAAGTGCAATCTTCTCCGTCTCGAGTTATCAAATTGTTTGGAGTTACTTATCACCTTCATAGCCACAGCTTGCCGCAATTTGGCCAGGATGCGGTTTGGGAATCATTGTATGAAAACTACAGAAAATCGACGCCTTATTCTAGAAAGGAGAGTCTTGTTAACCCTTGTGTACCTAGAGGATATAAGCTGACAGTGAATGCCAGTGATGAACAACTTCTAGTGTCTTCGCAGGGTGGAAACTTTTCTGAATGCAAGTCTCAAGTTTGGGCACTATTAAAGAGCAAACAAGATAAATGCGTGCAGGCACCTTGCACAATTGTCTCATCATTTCCTTTCGAGATAGGGGGCAAGCCAATTTCCGCAAACTTCTTATTTACTTCTGAGCTTTTTGGGCTTGTTCCCAGGGCTTCTTTGTTTGAGTTGGAGGCAGCTGGTCTACAATACTGTGAAGACGAATGGGACAAACAGAAAAATCAACATAATATTGTTGATGATTCAGATCTGCTGAAATATTGTTTCTCATCTGCTTATATGGTGGCGCTGTTGCATGACAGTCTAGGAATCCCCATGGATGAGAAAAGGGTTGGATTTGCAAACCAGACTGGAAATATTCTCCATGATTGGACTCTCGGTGCTTTCATTGTTGAAACAATGCTGGAACCCCTTGAATGGGAACTTGATAATAATGTGGGCCAGATTGTTGGAAATGAGTCTGTCACGTATTTCTCTGTCTTTGCATTTCTTTTAATAGCTGTGTTTGCTGTATTCTTTGTGTTGCAATCGCGGAAACCACAGTTAAAGACTATATATGATTTAGAAAAAGGACGCTATATTATTACACGTGTACGTAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko05169 map00230 map00240 map05169
Pfam Domains
Protein Families
GDA1_CD39

Protein Analysis

514

Amino Acids

57.05

Weight (kDa)

7.13

Isoelectric Point (pI)

49.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GDA1_CD39 PF01150 62 - 452 3e-58 GDA1/CD39 (nucleoside phosphatase) family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014823)

Species Orthologous Gene IDs
malus_domestica MD11G1180600.v1.1
prunus_persica Prupe.6G153100_v2.0.a1
pyrus_communis pycom11g15580
rosa_chinensis RchiOBHm_Chr5g0049751
rosa_laevigata RLG00000034644
rosa_multiflora Rmu_sc0016686.1_g000002 Rmu_sc0020783.1_g000001
rosa_roxburghii Rroxscaffold_1G00031680
rosa_rugosa Rorug05G0250800 Rorug05G0250800 Rorug05G0250900
rosa_samantha Rh5AG326900 Rh5BG338000 Rh5CG362600 Rh5DG350200
rosa_wichuraiana Rw0G018430 Rw5G030880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 376
Acc36I ACCTGC 2 cut(s) 376, 652
AccB1I GGYRCC 3 cut(s) 347, 388, 994
AccB7I CCANNNNNTGG 1 cut(s) 451
AccBSI CCGCTC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 1126
AccII CGCG 2 cut(s) 249, 1474
AcoI YGGCCR 1 cut(s) 772
AcsI RAATTY 1 cut(s) 179
AcuI CTGAAG 1 cut(s) 639
AcvI CACGTG 1 cut(s) 1532
AcyI GRCGYC 4 cut(s) 267, 339, 348, 821
AfaI GTAC 3 cut(s) 412, 861, 1536
AfiI CCNNNNNNNGG 6 cut(s) 451, 516, 770, 781, 1359, 1360
AflIII ACRYGT 2 cut(s) 1529, 1531
AgsI TTSAA 5 cut(s) 523, 607, 641, 1337, 1358
AjnI CCWGG 4 cut(s) 444, 456, 774, 1091
Alw21I GWGCWC 3 cut(s) 40, 444, 501
Alw26I GTCTC 6 cut(s) 58, 151, 550, 714, 951, 1015
Ama87I CYCGRG 2 cut(s) 479, 711
AoxI GGCC 4 cut(s) 454, 534, 772, 1381
ApeKI GCWGC 3 cut(s) 364, 418, 1118
ApoI RAATTY 1 cut(s) 179
Asp700I GAANNNNTTC 2 cut(s) 903, 931
AspLEI GCGC 2 cut(s) 350, 1231
AspS9I GGNCC 3 cut(s) 454, 534, 1381
AsuC2I CCSGG 2 cut(s) 437, 511
AsuHPI GGTGA 5 cut(s) 175, 211, 563, 589, 734
AvaI CYCGRG 2 cut(s) 479, 711
BaeGI GKGCMC 1 cut(s) 961
BalI TGGCCA 1 cut(s) 774
BanI GGYRCC 3 cut(s) 347, 388, 994
BanII GRGCYC 2 cut(s) 40, 444
BbrPI CACGTG 1 cut(s) 1532
BbsI GAAGAC 3 cut(s) 244, 906, 1146
Bbv12I GWGCWC 3 cut(s) 40, 444, 501
BbvI GCAGC 3 cut(s) 351, 405, 1130
BciT130I CCWGG 4 cut(s) 446, 458, 776, 1093
BcnI CCSGG 2 cut(s) 437, 511
BcoDI GTCTC 6 cut(s) 58, 151, 550, 714, 951, 1015
BfaI CTAG 4 cut(s) 831, 864, 908, 1247
BfmI CTRYAG 1 cut(s) 808
BfoI RGCGCY 2 cut(s) 351, 1232
BfuAI ACCTGC 2 cut(s) 376, 652
BglII AGATCT 2 cut(s) 503, 1189
BisI GCNGC 8 cut(s) 225, 327, 342, 362, 365, 419, 764, 1119
BlsI GCNGC 8 cut(s) 226, 328, 343, 363, 366, 420, 765, 1120
Bme1390I CCNGG 6 cut(s) 437, 446, 458, 511, 776, 1093
BmeT110I CYCGRG 2 cut(s) 479, 711
BmgT120I GGNCC 3 cut(s) 454, 534, 1381
BmiI GGNNCC 4 cut(s) 349, 390, 996, 1350
BmrFI CCNGG 6 cut(s) 437, 446, 458, 511, 776, 1093
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 2 cut(s) 252, 769
BmuI ACTGGG 1 cut(s) 639
BpiI GAAGAC 3 cut(s) 244, 906, 1146
BpmI CTGGAG 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 933
BpuMI CCSGG 2 cut(s) 437, 511
BsaAI YACGTR 3 cut(s) 1410, 1532, 1538
BsaBI GATNNNNATC 1 cut(s) 1188
BsaHI GRCGYC 4 cut(s) 267, 339, 348, 821
BsaI GGTCTC 1 cut(s) 550
BsaJI CCNNGG 4 cut(s) 435, 1091, 1092, 1257
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 6 cut(s) 451, 516, 770, 781, 1359, 1360
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 5 cut(s) 50, 408, 634, 891, 1297
Bse8I GATNNNNATC 1 cut(s) 1188
BseBI CCWGG 4 cut(s) 446, 458, 776, 1093
BseDI CCNNGG 4 cut(s) 435, 1091, 1092, 1257
BseGI GGATG 3 cut(s) 243, 784, 1267
BseJI GATNNNNATC 1 cut(s) 1188
BseLI CCNNNNNNNGG 6 cut(s) 451, 516, 770, 781, 1359, 1360
BseMII CTCAG 3 cut(s) 514, 561, 1062
BseNI ACTGG 5 cut(s) 50, 408, 634, 891, 1297
BseRI GAGGAG 7 cut(s) 55, 58, 61, 86, 107, 283, 307
BseSI GKGCMC 1 cut(s) 961
BseXI GCAGC 3 cut(s) 351, 405, 1130
BsgI GTGCAG 1 cut(s) 1010
Bsh1236I CGCG 2 cut(s) 249, 1474
BshFI GGCC 4 cut(s) 456, 536, 774, 1383
BshNI GGYRCC 3 cut(s) 347, 388, 994
BsiHKAI GWGCWC 3 cut(s) 40, 444, 501
BsiHKCI CYCGRG 2 cut(s) 479, 711
BsiSI CCGG 3 cut(s) 345, 437, 510
BslFI GGGAC 1 cut(s) 1163
BslI CCNNNNNNNGG 6 cut(s) 451, 516, 770, 781, 1359, 1360
BsmAI GTCTC 6 cut(s) 58, 151, 550, 714, 951, 1015
BsmBI CGTCTC 2 cut(s) 151, 714
BsmFI GGGAC 1 cut(s) 1163
BsmI GAATGC 2 cut(s) 892, 944
BsnI GGCC 4 cut(s) 456, 536, 774, 1383
Bso31I GGTCTC 1 cut(s) 550
BsoBI CYCGRG 2 cut(s) 479, 711
Bsp1286I GDGCHC 4 cut(s) 40, 444, 501, 961
Bsp143I GATC 3 cut(s) 462, 503, 1189
Bsp19I CCATGG 1 cut(s) 1257
BspANI GGCC 4 cut(s) 456, 536, 774, 1383
BspCNI CTCAG 3 cut(s) 513, 560, 1063
BspFNI CGCG 2 cut(s) 249, 1474
BspLI GGNNCC 4 cut(s) 349, 390, 996, 1350
BspMI ACCTGC 2 cut(s) 376, 652
BspQI GCTCTTC 1 cut(s) 549
BspT107I GGYRCC 3 cut(s) 347, 388, 994
BspTNI GGTCTC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 5 cut(s) 50, 408, 634, 891, 1297
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 4 cut(s) 435, 1091, 1092, 1257
BssMI GATC 3 cut(s) 462, 503, 1189
BssNI GRCGYC 4 cut(s) 267, 339, 348, 821
BssT1I CCWWGG 1 cut(s) 1257
Bst2UI CCWGG 4 cut(s) 446, 458, 776, 1093
Bst4CI ACNGT 4 cut(s) 883, 1136, 1244, 1485
Bst6I CTCTTC 6 cut(s) 26, 61, 140, 209, 549, 602
BstACI GRCGYC 4 cut(s) 267, 339, 348, 821
BstBAI YACGTR 3 cut(s) 1410, 1532, 1538
BstC8I GCNNGC 7 cut(s) 331, 346, 405, 761, 989, 993, 1040
BstDEI CTNAG 3 cut(s) 500, 547, 1071
BstDSI CCRYGG 1 cut(s) 1257
BstF5I GGATG 3 cut(s) 243, 784, 1267
BstFNI CGCG 2 cut(s) 249, 1474
BstH2I RGCGCY 2 cut(s) 351, 1232
BstHHI GCGC 2 cut(s) 350, 1231
BstKTI GATC 3 cut(s) 465, 506, 1192
BstMAI GTCTC 6 cut(s) 58, 151, 550, 714, 951, 1015
BstMBI GATC 3 cut(s) 462, 503, 1189
BstMWI GCNNNNNNNGC 6 cut(s) 84, 233, 347, 590, 1081, 1448
BstNI CCWGG 4 cut(s) 446, 458, 776, 1093
BstSCI CCNGG 6 cut(s) 435, 444, 456, 509, 774, 1091
BstSFI CTRYAG 1 cut(s) 808
BstSLI GKGCMC 1 cut(s) 961
BstSNI TACGTA 1 cut(s) 1538
BstUI CGCG 2 cut(s) 249, 1474
BstV1I GCAGC 3 cut(s) 351, 405, 1130
BstV2I GAAGAC 3 cut(s) 244, 906, 1146
BstX2I RGATCY 2 cut(s) 503, 1189
BstXI CCANNNNNNTGG 1 cut(s) 1391
BstYI RGATCY 2 cut(s) 503, 1189
BsuRI GGCC 4 cut(s) 456, 536, 774, 1383
BtgI CCRYGG 1 cut(s) 1257
BtsCI GGATG 3 cut(s) 243, 784, 1267
BtsIMutI CAGTG 3 cut(s) 627, 888, 898
BveI ACCTGC 2 cut(s) 376, 652
Cac8I GCNNGC 7 cut(s) 331, 346, 405, 761, 989, 993, 1040
CfoI GCGC 2 cut(s) 350, 1231
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 454, 534, 1381
CseI GACGC 5 cut(s) 238, 275, 347, 829, 1524
Csp6I GTAC 3 cut(s) 411, 860, 1535
CviAII CATG 3 cut(s) 1238, 1258, 1307
CviQI GTAC 3 cut(s) 411, 860, 1535
DdeI CTNAG 3 cut(s) 500, 547, 1071
DinI GGCGCC 1 cut(s) 349
DpnI GATC 3 cut(s) 464, 505, 1191
DpnII GATC 3 cut(s) 462, 503, 1189
EaeI YGGCCR 1 cut(s) 772
Eam1104I CTCTTC 6 cut(s) 26, 61, 140, 209, 549, 602
EarI CTCTTC 6 cut(s) 26, 61, 140, 209, 549, 602
Ecl136II GAGCTC 2 cut(s) 38, 442
Eco105I TACGTA 1 cut(s) 1538
Eco130I CCWWGG 1 cut(s) 1257
Eco24I GRGCYC 2 cut(s) 40, 444
Eco31I GGTCTC 1 cut(s) 550
Eco53kI GAGCTC 2 cut(s) 38, 442
Eco57I CTGAAG 1 cut(s) 639
Eco72I CACGTG 1 cut(s) 1532
Eco88I CYCGRG 2 cut(s) 479, 711
EcoICRI GAGCTC 2 cut(s) 38, 442
EcoRII CCWGG 4 cut(s) 444, 456, 774, 1091
EcoT14I CCWWGG 1 cut(s) 1257
EcoT38I GRGCYC 2 cut(s) 40, 444
EgeI GGCGCC 1 cut(s) 349
EheI GGCGCC 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 1257
Esp3I CGTCTC 2 cut(s) 151, 714
FaeI CATG 3 cut(s) 1241, 1261, 1310
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 1163
FatI CATG 3 cut(s) 1237, 1257, 1306
FauI CCCGC 4 cut(s) 248, 305, 316, 338
FblI GTMKAC 1 cut(s) 1126
Fnu4HI GCNGC 8 cut(s) 225, 327, 342, 362, 365, 419, 764, 1119
FokI GGATG 3 cut(s) 230, 791, 1274
FriOI GRGCYC 2 cut(s) 40, 444
Fsp4HI GCNGC 8 cut(s) 225, 327, 342, 362, 365, 419, 764, 1119
FspBI CTAG 4 cut(s) 831, 864, 908, 1247
GlaI GCGC 2 cut(s) 349, 1230
GluI GCNGC 8 cut(s) 225, 327, 342, 362, 365, 419, 764, 1119
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 2 cut(s) 351, 1232
HaeIII GGCC 4 cut(s) 456, 536, 774, 1383
HapII CCGG 3 cut(s) 345, 437, 510
HgaI GACGC 5 cut(s) 238, 275, 347, 829, 1524
HhaI GCGC 2 cut(s) 350, 1231
Hin1I GRCGYC 4 cut(s) 267, 339, 348, 821
Hin1II CATG 3 cut(s) 1241, 1261, 1310
Hin6I GCGC 2 cut(s) 348, 1229
HinP1I GCGC 2 cut(s) 348, 1229
HincII GTYRAC 2 cut(s) 12, 850
HindII GTYRAC 2 cut(s) 12, 850
HindIII AAGCTT 1 cut(s) 473
HinfI GANTC 8 cut(s) 194, 482, 791, 841, 1184, 1251, 1315, 1400
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 3 cut(s) 345, 437, 510
HphI GGTGA 5 cut(s) 175, 211, 563, 589, 734
Hpy166II GTNNAC 5 cut(s) 12, 850, 860, 1127, 1535
Hpy188I TCNGA 6 cut(s) 193, 503, 599, 937, 1072, 1189
Hpy188III TCNNGA 4 cut(s) 479, 711, 831, 1027
Hpy8I GTNNAC 5 cut(s) 12, 850, 860, 1127, 1535
Hpy99I CGWCG 3 cut(s) 269, 341, 823
HpyAV CCTTC 4 cut(s) 139, 331, 487, 755
HpyCH4III ACNGT 4 cut(s) 883, 1136, 1244, 1485
HpyCH4IV ACGT 3 cut(s) 1409, 1531, 1537
HpyCH4V TGCA 9 cut(s) 661, 697, 942, 991, 1002, 1237, 1283, 1427, 1468
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 233, 347, 590, 1081, 1448
HpyF3I CTNAG 3 cut(s) 500, 547, 1071
HpySE526I ACGT 3 cut(s) 1409, 1531, 1537
Hsp92I GRCGYC 4 cut(s) 267, 339, 348, 821
Hsp92II CATG 3 cut(s) 1241, 1261, 1310
HspAI GCGC 2 cut(s) 348, 1229
KasI GGCGCC 1 cut(s) 347
KroI GCCGGC 1 cut(s) 344
KroNI GCCGGC 1 cut(s) 346
KspAI GTTAAC 1 cut(s) 850
Kzo9I GATC 3 cut(s) 462, 503, 1189
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 5 cut(s) 43, 168, 372, 439, 447
Lsp1109I GCAGC 3 cut(s) 351, 405, 1130
LweI GCATC 2 cut(s) 252, 769
MaeI CTAG 4 cut(s) 831, 864, 908, 1247
MaeII ACGT 3 cut(s) 1409, 1531, 1537
MaeIII GTNAC 3 cut(s) 664, 733, 1405
MalI GATC 3 cut(s) 464, 505, 1191
MbiI CCGCTC 1 cut(s) 300
MboI GATC 3 cut(s) 462, 503, 1189
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 2 cut(s) 503, 1189
MhlI GDGCHC 4 cut(s) 40, 444, 501, 961
MlsI TGGCCA 1 cut(s) 774
MluCI AATT 6 cut(s) 179, 586, 722, 767, 1005, 1044
MluNI TGGCCA 1 cut(s) 774
Mly113I GGCGCC 1 cut(s) 348
MlyI GAGTC 4 cut(s) 491, 850, 1309, 1409
MmeI TCCRAC 3 cut(s) 1092, 1255, 1372
Mox20I TGGCCA 1 cut(s) 774
MreI CGCCGGCG 1 cut(s) 344
MroNI GCCGGC 1 cut(s) 344
MroXI GAANNNNTTC 2 cut(s) 903, 931
MscI TGGCCA 1 cut(s) 774
MseI TTAA 4 cut(s) 849, 965, 1436, 1487
Msp20I TGGCCA 1 cut(s) 774
MspA1I CMGCKG 2 cut(s) 364, 1121
MspI CCGG 3 cut(s) 345, 437, 510
MspR9I CCNGG 6 cut(s) 437, 446, 458, 511, 776, 1093
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 2 cut(s) 892, 944
MvaI CCWGG 4 cut(s) 446, 458, 776, 1093
MvnI CGCG 2 cut(s) 249, 1474
MwoI GCNNNNNNNGC 6 cut(s) 84, 233, 347, 590, 1081, 1448
NaeI GCCGGC 1 cut(s) 346
NarI GGCGCC 1 cut(s) 348
NciI CCSGG 2 cut(s) 437, 511
NcoI CCATGG 1 cut(s) 1257
NdeII GATC 3 cut(s) 462, 503, 1189
NgoMIV GCCGGC 1 cut(s) 344
NlaIII CATG 3 cut(s) 1241, 1261, 1310
NlaIV GGNNCC 4 cut(s) 349, 390, 996, 1350
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 1405
PaeR7I CTCGAG 2 cut(s) 479, 711
PaqCI CACCTGC 1 cut(s) 376
PasI CCCWGGG 1 cut(s) 1092
PciSI GCTCTTC 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 389
PctI GAATGC 2 cut(s) 892, 944
PdiI GCCGGC 1 cut(s) 346
PdmI GAANNNNTTC 2 cut(s) 903, 931
PfeI GAWTC 4 cut(s) 194, 791, 1184, 1251
PflMI CCANNNNNTGG 1 cut(s) 451
PfoI TCCNGGA 1 cut(s) 444
PkrI GCNGC 8 cut(s) 226, 328, 343, 363, 366, 420, 765, 1120
PleI GAGTC 4 cut(s) 490, 849, 1309, 1408
PluTI GGCGCC 1 cut(s) 351
PmaCI CACGTG 1 cut(s) 1532
PmlI CACGTG 1 cut(s) 1532
PpsI GAGTC 4 cut(s) 490, 849, 1309, 1408
Ppu21I YACGTR 3 cut(s) 1410, 1532, 1538
Psp124BI GAGCTC 2 cut(s) 40, 444
Psp6I CCWGG 4 cut(s) 444, 456, 774, 1091
PspCI CACGTG 1 cut(s) 1532
PspGI CCWGG 4 cut(s) 444, 456, 774, 1091
PspN4I GGNNCC 4 cut(s) 349, 390, 996, 1350
PspPI GGNCC 3 cut(s) 454, 534, 1381
PsuI RGATCY 2 cut(s) 503, 1189
PvuII CAGCTG 1 cut(s) 1121
RsaI GTAC 3 cut(s) 412, 861, 1536
RsaNI GTAC 3 cut(s) 411, 860, 1535
SacI GAGCTC 2 cut(s) 40, 444
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 4 cut(s) 849, 965, 1436, 1487
SatI GCNGC 8 cut(s) 225, 327, 342, 362, 365, 419, 764, 1119
Sau3AI GATC 3 cut(s) 462, 503, 1189
Sau96I GGNCC 3 cut(s) 454, 534, 1381
SchI GAGTC 4 cut(s) 491, 850, 1309, 1409
ScrFI CCNGG 6 cut(s) 437, 446, 458, 511, 776, 1093
SduI GDGCHC 4 cut(s) 40, 444, 501, 961
SfaNI GCATC 2 cut(s) 252, 769
SfcI CTRYAG 1 cut(s) 808
SfoI GGCGCC 1 cut(s) 349
Sfr274I CTCGAG 2 cut(s) 479, 711
SgrAI CRCCGGYG 1 cut(s) 344
SlaI CTCGAG 2 cut(s) 479, 711
SmlI CTYRAG 3 cut(s) 479, 711, 948
SmoI CTYRAG 3 cut(s) 479, 711, 948
SnaBI TACGTA 1 cut(s) 1538
Sse9I AATT 6 cut(s) 179, 586, 722, 767, 1005, 1044
SspDI GGCGCC 1 cut(s) 347
SspI AATATT 3 cut(s) 1174, 1202, 1300
SspMI CTAG 4 cut(s) 831, 864, 908, 1247
SstI GAGCTC 2 cut(s) 40, 444
StyD4I CCNGG 6 cut(s) 435, 444, 456, 509, 774, 1091
StyI CCWWGG 1 cut(s) 1257
TaaI ACNGT 4 cut(s) 883, 1136, 1244, 1485
TaiI ACGT 3 cut(s) 1412, 1534, 1540
TaqI TCGA 6 cut(s) 29, 264, 480, 712, 818, 1026
TasI AATT 6 cut(s) 179, 586, 722, 767, 1005, 1044
TauI GCSGC 5 cut(s) 227, 329, 344, 364, 766
TfiI GAWTC 4 cut(s) 194, 791, 1184, 1251
Tru1I TTAA 4 cut(s) 849, 965, 1436, 1487
Tru9I TTAA 4 cut(s) 849, 965, 1436, 1487
TscAI CASTG 3 cut(s) 634, 888, 898
TseFI GTSAC 1 cut(s) 1405
TseI GCWGC 3 cut(s) 364, 418, 1118
Tsp45I GTSAC 1 cut(s) 1405
TspDTI ATGAA 5 cut(s) 410, 737, 816, 912, 1318
TspGWI ACGGA 1 cut(s) 696
TspRI CASTG 3 cut(s) 634, 888, 898
Van91I CCANNNNNTGG 1 cut(s) 451
XapI RAATTY 1 cut(s) 179
XbaI TCTAGA 1 cut(s) 830
XhoI CTCGAG 2 cut(s) 479, 711
XmiI GTMKAC 1 cut(s) 1126
XmnI GAANNNNTTC 2 cut(s) 903, 931
XspI CTAG 4 cut(s) 831, 864, 908, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.