Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp07

Orthogroup ID

OG0010083

Physical Location & Seq

Genomic Coordinates Reverse (-)

18656455 .. 18658092

Gene Structure 1638 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.7G198600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1638 bp

ATGACCCTCTTGTTCTTCACAGCCCGTCCCACCTTTTGGGTTCGAGCCTCCAAGATCGCCATTTCCCATTTCCACAGTCTTGCCCATGGAGGAGCTCGACCTCAAATAGAGGTAATATCAAACCCAGAGGCTTGGTTTGTTAAGGTTGTTTGCACACTCTTTGTTCGCTCGCATGCTTTGGATTCTTACTTGGGTTATCTTAGTAAGAACTTGACGCCTTCTATTGCTTTCGAGGTTATTAGACGGCTAAACCATCCGAAACTGGGGTTGAAGTTCTTTGAGCTTAGTAGGCTCAGTTTGAGTGTTAACCATAGTGTATGGACGTACAATTTTCTTTTGAGGTCTCTATGTCAAATTGGTCTTCAAGATTCTGCTAAGTTGGTGTTTGATTACATGAGGAGTGACGGTCATACGCCTGATGATTCGATTGCAGAACTATTGGTATCATCCTATGCCCAAATGGGCAAGTTAAATAACGCTGAGAAACTTCTTGATGAGGTTCACTGTGGTGAGGTTAGATTGTCGCCTTTGGTGTACAATAACTTGTTCAATGTGTTGGTTAAGCAGAATAAAGTAGAGGAGGCTGTTTGCTTATTTAGAAAGCATATGGGTTCGCATTGTCGTCCAGATAGTTGGACTTTCAATATTCTAATTCGAGGTTTATGCAGAATTGGAGAAATTGATAAGGCTTTTGAGTTTTTCAGTGATATGGAGAGTTTTGGTTGTTACCCTGATATCGTTACATATAACACACTAATTAGTGGACTTTGTAGGGCTAATGAGGTAGATAGAGGGTGTCATTTACTTAAGGAGGTTCAGTCAAGAATCGAACTTTCACCGGATGTTATAACTTTTACGTCAGTCATATCAGGCTACTGCAAATTGGGTAAGATGGAGGAGGCCTCTGTTCTTTTTGATGAGATGAATAATTCTGGAGTTGGACCAACTTCTGTTACTTTTAATGCATTGATTGATGGATTTGGTAAGTCTGGTAACATGATTTCTGCACGTGCTATGTATGAGAAAATGCTCTTTCATGGTTATCGTCCGGATGTTATAACCTTCACTTCCCTAATTGATGGCTATTGTCGAGCTGGGAAACTGAGTCAGGGATTAAAGCTTTGGCAAGAGATGAATGCAAAAAATGTATCTCCAAGTGCATATACGTTCTCTGTCCTTATTAATGCTTTGTGCAGGGAGAATAGACTACAGGAGGCGCGTGAGTTTCTGAGGCAATTGAAGAGGAGTAATGTCATTCCACGACCTTTTATATATAACCCTGTGATAGATGGATTTTGTAAGGCTGGCAATGTTGATGAGGCAAACCTAATTGTGGCAGAGATGGAGGAGAAGAGATGTAGCCCTGATAAAGTGACATTTACCATTCTCATACTCGGGAATTGTATGAAAGGGAGAATGTCAGAGGCAATTAGCAACTTTAAAAAAATGTTGGCGATTGGATGTGCCCCAGACAACATCACTGTAGATTCTTTGATATCTTGCCTCATGAAGGCTGGGATGCCTAATGAAGCCCATCACATTAAGAAAATTGCATGTGAGGATCTGAATTTGGGTATGTCACCTTCACGAAGAGCTGATCATTTGAGAGCAAATGCAAAAATTACAGTGGCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

546

Amino Acids

61.15

Weight (kDa)

8.53

Isoelectric Point (pI)

41.95

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010083)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G06000 AT2G06000
fragaria_vesca	FvH4_5g31880
malus_domestica	MD02G1091700.v1.1 MD15G1217800.v1.1
prunus_persica	Prupe.7G198600_v2.0.a1
pyrus_communis	pycom02g07300 pycom15g19340 pycom15g33430 pycom15g33440
rosa_chinensis	RchiOBHm_Chr7g0227901
rosa_laevigata	RLG00000001605
rosa_multiflora	Rmu_co8402659.1_g000001 Rmu_sc0000347.1_g000003
rosa_roxburghii	Rroxscaffold_3G00231780
rosa_rugosa	Rorug07G0244100
rosa_samantha	Rh7AG397500 Rh7BG379100 Rh7CG415700 Rh7DG393000
rosa_wichuraiana	Rw7G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	848, 1058
AccB7I	CCANNNNNTGG	1 cut(s)	36
AccII	CGCG	1 cut(s)	1219
AccIII	TCCGGA	1 cut(s)	1048
AclWI	GGATC	1 cut(s)	1569
AcsI	RAATTY	1 cut(s)	1567
AcvI	CACGTG	1 cut(s)	1010
AcyI	GRCGYC	1 cut(s)	215
AfaI	GTAC	2 cut(s)	326, 536
AfiI	CCNNNNNNNGG	4 cut(s)	36, 263, 1333, 1510
AflII	CTTAAG	1 cut(s)	806
AgsI	TTSAA	5 cut(s)	271, 365, 550, 643, 1240
AleI	CACNNNNGTG	1 cut(s)	507
AluBI	AGCT	5 cut(s)	95, 283, 1094, 1120, 1595
AluI	AGCT	5 cut(s)	95, 283, 1094, 1120, 1595
Alw21I	GWGCWC	1 cut(s)	97
Alw26I	GTCTC	1 cut(s)	348
AlwI	GGATC	1 cut(s)	1569
AlwNI	CAGNNNCTG	1 cut(s)	1631
Ama87I	CYCGRG	1 cut(s)	1394
Aor13HI	TCCGGA	1 cut(s)	1048
AoxI	GGCC	1 cut(s)	900
ApoI	RAATTY	1 cut(s)	1567
AseI	ATTAAT	1 cut(s)	1182
AspLEI	GCGC	1 cut(s)	1219
AspS9I	GGNCC	1 cut(s)	941
AsuHPI	GGTGA	3 cut(s)	521, 828, 1572
AvaI	CYCGRG	1 cut(s)	1394
AvaII	GGWCC	1 cut(s)	941
BaeGI	GKGCMC	1 cut(s)	1468
BanII	GRGCYC	1 cut(s)	97
BarI	GAAGNNNNNNTAC	2 cut(s)	1567, 1599
BbrPI	CACGTG	1 cut(s)	1010
BbsI	GAAGAC	1 cut(s)	353
Bbv12I	GWGCWC	1 cut(s)	97
BccI	CCATC	7 cut(s)	261, 886, 968, 1073, 1283, 1336, 1542
BceAI	ACGGC	1 cut(s)	260
BcgI	CGANNNNNNTGC	2 cut(s)	645, 679
BclI	TGATCA	1 cut(s)	1597
BcoDI	GTCTC	1 cut(s)	348
BfmI	CTRYAG	2 cut(s)	1208, 1482
BfrI	CTTAAG	1 cut(s)	806
Bme18I	GGWCC	1 cut(s)	941
BmeT110I	CYCGRG	1 cut(s)	1394
BmgT120I	GGNCC	1 cut(s)	941
BmrI	ACTGGG	1 cut(s)	272
BmsI	GCATC	1 cut(s)	1509
BmuI	ACTGGG	1 cut(s)	272
BpiI	GAAGAC	1 cut(s)	353
BplI	GAGNNNNNCTC	2 cut(s)	887, 919
BpmI	CTGGAG	1 cut(s)	954
BsaAI	YACGTR	1 cut(s)	1010
BsaHI	GRCGYC	1 cut(s)	215
BsaI	GGTCTC	1 cut(s)	348
BsaJI	CCNNGG	1 cut(s)	85
BsaWI	WCCGGW	2 cut(s)	838, 1048
BsaXI	ACNNNNNCTCC	4 cut(s)	391, 421, 803, 833
Bsc4I	CCNNNNNNNGG	4 cut(s)	36, 263, 1333, 1510
Bse1I	ACTGG	1 cut(s)	267
Bse3DI	GCAATG	1 cut(s)	1315
BseAI	TCCGGA	1 cut(s)	1048
BseDI	CCNNGG	1 cut(s)	85
BseGI	GGATG	6 cut(s)	253, 446, 847, 1057, 1466, 1524
BseLI	CCNNNNNNNGG	4 cut(s)	36, 263, 1333, 1510
BseMI	GCAATG	1 cut(s)	1315
BseMII	CTCAG	4 cut(s)	307, 471, 1094, 1220
BseNI	ACTGG	1 cut(s)	267
BseRI	GAGGAG	6 cut(s)	105, 412, 593, 911, 1258, 1361
BseSI	GKGCMC	1 cut(s)	1468
BseYI	CCCAGC	2 cut(s)	1094, 1514
BsgI	GTGCAG	2 cut(s)	990, 1213
Bsh1236I	CGCG	1 cut(s)	1219
BshFI	GGCC	1 cut(s)	902
BsiHKAI	GWGCWC	1 cut(s)	97
BsiHKCI	CYCGRG	1 cut(s)	1394
BsiSI	CCGG	2 cut(s)	839, 1049
BslFI	GGGAC	1 cut(s)	12
BslI	CCNNNNNNNGG	4 cut(s)	36, 263, 1333, 1510
BsmAI	GTCTC	1 cut(s)	348
BsmFI	GGGAC	1 cut(s)	12
BsmI	GAATGC	1 cut(s)	1141
BsnI	GGCC	1 cut(s)	902
Bso31I	GGTCTC	1 cut(s)	348
BsoBI	CYCGRG	1 cut(s)	1394
Bsp1286I	GDGCHC	2 cut(s)	97, 1468
Bsp13I	TCCGGA	1 cut(s)	1048
Bsp1407I	TGTACA	1 cut(s)	534
Bsp143I	GATC	3 cut(s)	54, 1561, 1597
Bsp19I	CCATGG	1 cut(s)	85
BspANI	GGCC	1 cut(s)	902
BspCNI	CTCAG	4 cut(s)	306, 472, 1095, 1221
BspEI	TCCGGA	1 cut(s)	1048
BspFNI	CGCG	1 cut(s)	1219
BspHI	TCATGA	1 cut(s)	1506
BspPI	GGATC	1 cut(s)	1569
BspQI	GCTCTTC	1 cut(s)	1585
BspTI	CTTAAG	1 cut(s)	806
BspTNI	GGTCTC	1 cut(s)	348
BsrDI	GCAATG	1 cut(s)	1315
BsrGI	TGTACA	1 cut(s)	534
BsrI	ACTGG	1 cut(s)	267
BssECI	CCNNGG	1 cut(s)	85
BssMI	GATC	3 cut(s)	54, 1561, 1597
BssNI	GRCGYC	1 cut(s)	215
BssT1I	CCWWGG	1 cut(s)	85
Bst4CI	ACNGT	5 cut(s)	77, 407, 506, 1483, 1627
Bst6I	CTCTTC	3 cut(s)	1235, 1346, 1585
BstACI	GRCGYC	1 cut(s)	215
BstAFI	CTTAAG	1 cut(s)	806
BstAUI	TGTACA	1 cut(s)	534
BstBAI	YACGTR	1 cut(s)	1010
BstC8I	GCNNGC	3 cut(s)	170, 174, 1306
BstDEI	CTNAG	7 cut(s)	200, 284, 293, 375, 480, 1103, 1229
BstDSI	CCRYGG	1 cut(s)	85
BstENI	CCTNNNNNAGG	1 cut(s)	1508
BstF5I	GGATG	6 cut(s)	253, 446, 847, 1057, 1466, 1524
BstFNI	CGCG	1 cut(s)	1219
BstHHI	GCGC	1 cut(s)	1219
BstKTI	GATC	3 cut(s)	57, 1564, 1600
BstMAI	GTCTC	1 cut(s)	348
BstMBI	GATC	3 cut(s)	54, 1561, 1597
BstMWI	GCNNNNNNNGC	1 cut(s)	289
BstNSI	RCATGY	2 cut(s)	176, 1557
BstSFI	CTRYAG	2 cut(s)	1208, 1482
BstSLI	GKGCMC	1 cut(s)	1468
BstUI	CGCG	1 cut(s)	1219
BstV2I	GAAGAC	1 cut(s)	353
BstX2I	RGATCY	1 cut(s)	1561
BstXI	CCANNNNNNTGG	2 cut(s)	132, 633
BstYI	RGATCY	1 cut(s)	1561
BsuRI	GGCC	1 cut(s)	902
BtgI	CCRYGG	1 cut(s)	85
BtsCI	GGATG	6 cut(s)	253, 446, 847, 1057, 1466, 1524
BtsIMutI	CAGTG	4 cut(s)	502, 709, 1479, 1632
Cac8I	GCNNGC	3 cut(s)	170, 174, 1306
CaiI	CAGNNNCTG	1 cut(s)	1631
CciI	TCATGA	1 cut(s)	1506
CfoI	GCGC	1 cut(s)	1219
Cfr13I	GGNCC	1 cut(s)	941
CseI	GACGC	1 cut(s)	223
Csp6I	GTAC	2 cut(s)	325, 535
CviAII	CATG	7 cut(s)	86, 173, 394, 997, 1037, 1507, 1554
CviQI	GTAC	2 cut(s)	325, 535
DdeI	CTNAG	7 cut(s)	200, 284, 293, 375, 480, 1103, 1229
DpnI	GATC	3 cut(s)	56, 1563, 1599
DpnII	GATC	3 cut(s)	54, 1561, 1597
DraI	TTTAAA	1 cut(s)	1441
Eam1104I	CTCTTC	3 cut(s)	1235, 1346, 1585
EarI	CTCTTC	3 cut(s)	1235, 1346, 1585
Ecl136II	GAGCTC	1 cut(s)	95
Eco130I	CCWWGG	1 cut(s)	85
Eco147I	AGGCCT	1 cut(s)	902
Eco24I	GRGCYC	1 cut(s)	97
Eco31I	GGTCTC	1 cut(s)	348
Eco32I	GATATC	2 cut(s)	736, 1497
Eco47I	GGWCC	1 cut(s)	941
Eco53kI	GAGCTC	1 cut(s)	95
Eco72I	CACGTG	1 cut(s)	1010
Eco88I	CYCGRG	1 cut(s)	1394
EcoICRI	GAGCTC	1 cut(s)	95
EcoNI	CCTNNNNNAGG	1 cut(s)	1508
EcoRV	GATATC	2 cut(s)	736, 1497
EcoT14I	CCWWGG	1 cut(s)	85
EcoT22I	ATGCAT	1 cut(s)	967
EcoT38I	GRGCYC	1 cut(s)	97
ErhI	CCWWGG	1 cut(s)	85
FaeI	CATG	7 cut(s)	89, 176, 397, 1000, 1040, 1510, 1557
FaqI	GGGAC	1 cut(s)	12
FatI	CATG	7 cut(s)	85, 172, 393, 996, 1036, 1506, 1553
FauNDI	CATATG	1 cut(s)	606
FbaI	TGATCA	1 cut(s)	1597
FokI	GGATG	6 cut(s)	240, 433, 854, 1064, 1473, 1531
FriOI	GRGCYC	1 cut(s)	97
GlaI	GCGC	1 cut(s)	1218
GsaI	CCCAGC	2 cut(s)	1098, 1518
GsuI	CTGGAG	1 cut(s)	954
HaeIII	GGCC	1 cut(s)	902
HapII	CCGG	2 cut(s)	839, 1049
HgaI	GACGC	1 cut(s)	223
HhaI	GCGC	1 cut(s)	1219
Hin1I	GRCGYC	1 cut(s)	215
Hin1II	CATG	7 cut(s)	89, 176, 397, 1000, 1040, 1510, 1557
Hin6I	GCGC	1 cut(s)	1217
HinP1I	GCGC	1 cut(s)	1217
HincII	GTYRAC	1 cut(s)	307
HindII	GTYRAC	1 cut(s)	307
HindIII	AAGCTT	1 cut(s)	1118
HinfI	GANTC	6 cut(s)	182, 368, 422, 825, 1105, 1487
HpaI	GTTAAC	1 cut(s)	307
HpaII	CCGG	2 cut(s)	839, 1049
HphI	GGTGA	3 cut(s)	521, 828, 1572
Hpy166II	GTNNAC	4 cut(s)	307, 502, 535, 764
Hpy188I	TCNGA	4 cut(s)	258, 1230, 1423, 1566
Hpy188III	TCNNGA	9 cut(s)	365, 491, 626, 822, 933, 1049, 1396, 1507, 1587
Hpy8I	GTNNAC	4 cut(s)	307, 502, 535, 764
HpyAV	CCTTC	4 cut(s)	228, 1072, 1504, 1593
HpyCH4III	ACNGT	5 cut(s)	77, 407, 506, 1483, 1627
HpyCH4IV	ACGT	4 cut(s)	323, 857, 1009, 1166
HpyF10VI	GCNNNNNNNGC	1 cut(s)	289
HpyF3I	CTNAG	7 cut(s)	200, 284, 293, 375, 480, 1103, 1229
HpySE526I	ACGT	4 cut(s)	323, 857, 1009, 1166
Hsp92I	GRCGYC	1 cut(s)	215
Hsp92II	CATG	7 cut(s)	89, 176, 397, 1000, 1040, 1510, 1557
HspAI	GCGC	1 cut(s)	1217
Kpn2I	TCCGGA	1 cut(s)	1048
Ksp22I	TGATCA	1 cut(s)	1597
KspAI	GTTAAC	1 cut(s)	307
Kzo9I	GATC	3 cut(s)	54, 1561, 1597
LguI	GCTCTTC	1 cut(s)	1585
LmnI	GCTCC	1 cut(s)	92
LweI	GCATC	1 cut(s)	1509
MaeII	ACGT	4 cut(s)	323, 857, 1009, 1166
MaeIII	GTNAC	7 cut(s)	401, 725, 739, 952, 992, 1372, 1578
MalI	GATC	3 cut(s)	56, 1563, 1599
MboI	GATC	3 cut(s)	54, 1561, 1597
MboII	GAAGA	5 cut(s)	7, 353, 1252, 1363, 1602
MfeI	CAATTG	1 cut(s)	1235
MflI	RGATCY	1 cut(s)	1561
MhlI	GDGCHC	2 cut(s)	97, 1468
MlyI	GAGTC	1 cut(s)	1114
MmeI	TCCRAC	2 cut(s)	614, 919
Mph1103I	ATGCAT	1 cut(s)	967
MroI	TCCGGA	1 cut(s)	1048
MslI	CAYNNNNRTG	1 cut(s)	507
MspCI	CTTAAG	1 cut(s)	806
MspI	CCGG	2 cut(s)	839, 1049
MunI	CAATTG	1 cut(s)	1235
Mva1269I	GAATGC	1 cut(s)	1141
MvnI	CGCG	1 cut(s)	1219
MwoI	GCNNNNNNNGC	1 cut(s)	289
NcoI	CCATGG	1 cut(s)	85
NdeI	CATATG	1 cut(s)	606
NdeII	GATC	3 cut(s)	54, 1561, 1597
NlaIII	CATG	7 cut(s)	89, 176, 397, 1000, 1040, 1510, 1557
NmuCI	GTSAC	3 cut(s)	401, 1372, 1578
NsiI	ATGCAT	1 cut(s)	967
NspI	RCATGY	2 cut(s)	176, 1557
OliI	CACNNNNGTG	1 cut(s)	507
PaeI	GCATGC	1 cut(s)	176
PagI	TCATGA	1 cut(s)	1506
PceI	AGGCCT	1 cut(s)	902
PciSI	GCTCTTC	1 cut(s)	1585
PctI	GAATGC	1 cut(s)	1141
PfeI	GAWTC	5 cut(s)	182, 368, 422, 825, 1487
PflMI	CCANNNNNTGG	1 cut(s)	36
PleI	GAGTC	1 cut(s)	1113
PmaCI	CACGTG	1 cut(s)	1010
PmlI	CACGTG	1 cut(s)	1010
PpsI	GAGTC	1 cut(s)	1113
Ppu21I	YACGTR	1 cut(s)	1010
PshBI	ATTAAT	1 cut(s)	1182
PsiI	TTATAA	2 cut(s)	848, 1058
Psp124BI	GAGCTC	1 cut(s)	97
PspCI	CACGTG	1 cut(s)	1010
PspFI	CCCAGC	2 cut(s)	1094, 1514
PspPI	GGNCC	1 cut(s)	941
PstNI	CAGNNNCTG	1 cut(s)	1631
PsuI	RGATCY	1 cut(s)	1561
RsaI	GTAC	2 cut(s)	326, 536
RsaNI	GTAC	2 cut(s)	325, 535
RseI	CAYNNNNRTG	1 cut(s)	507
SacI	GAGCTC	1 cut(s)	97
SapI	GCTCTTC	1 cut(s)	1585
Sau3AI	GATC	3 cut(s)	54, 1561, 1597
Sau96I	GGNCC	1 cut(s)	941
SchI	GAGTC	1 cut(s)	1114
SduI	GDGCHC	2 cut(s)	97, 1468
SfaNI	GCATC	1 cut(s)	1509
SfcI	CTRYAG	2 cut(s)	1208, 1482
SinI	GGWCC	1 cut(s)	941
SmiMI	CAYNNNNRTG	1 cut(s)	507
SmlI	CTYRAG	1 cut(s)	806
SmoI	CTYRAG	1 cut(s)	806
SphI	GCATGC	1 cut(s)	176
SseBI	AGGCCT	1 cut(s)	902
SspI	AATATT	1 cut(s)	646
SstI	GAGCTC	1 cut(s)	97
StuI	AGGCCT	1 cut(s)	902
StyI	CCWWGG	1 cut(s)	85
TaaI	ACNGT	5 cut(s)	77, 407, 506, 1483, 1627
TaiI	ACGT	4 cut(s)	326, 860, 1012, 1169
TaqI	TCGA	7 cut(s)	43, 97, 231, 425, 655, 828, 1090
TatI	WGTACW	1 cut(s)	534
TfiI	GAWTC	5 cut(s)	182, 368, 422, 825, 1487
TscAI	CASTG	4 cut(s)	509, 709, 1486, 1632
TseFI	GTSAC	3 cut(s)	401, 1372, 1578
Tsp45I	GTSAC	3 cut(s)	401, 1372, 1578
TspDTI	ATGAA	6 cut(s)	938, 1025, 1148, 1421, 1523, 1542
TspRI	CASTG	4 cut(s)	509, 709, 1486, 1632
Van91I	CCANNNNNTGG	1 cut(s)	36
Vha464I	CTTAAG	1 cut(s)	806
VpaK11BI	GGWCC	1 cut(s)	941
VspI	ATTAAT	1 cut(s)	1182
XagI	CCTNNNNNAGG	1 cut(s)	1508
XapI	RAATTY	1 cut(s)	1567
XceI	RCATGY	2 cut(s)	176, 1557
Zsp2I	ATGCAT	1 cut(s)	967

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.