Rorug07G0244100

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010083
Physical Location & Seq
Reverse (-)
22543798 .. 22552064
8267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0244100.1

Sequence Viewer

Length: 1392 bp
ATGGATTCTGTTCCCAAGTCTTTCAATCCAAACAGGCGTCTCAAGGAAGAATTCGTCAGCAATTTGACTGGATCTTCCATGCTGGAAATCGCCGCCATCACCACAATAATCCCAGCTCTGTTCCTGCTGCGGCGAGCAGTCGGCTTCAATCGTCCTGCTGATGATGTCACATTGAAGAAAAATGATGATGCAAAAGTTGGTTCTAAGAGTTGGCAGGCTTACATGGCTAGGCTGGCCGTGGATTTTCTATTCATTATGGTTCCATTTGTCTTAATTTTCACTGTCTTATCAGAGTGGATGTATATCTGGGCAACTTTGCTAATTCTGTTACTTGTTTTCAGCATTGCAGCCAGAAGTTTTGGTTTTGGTTCTACTTTGGAAGAAGAACCCGATTCTATTAGGACAAGTATTACATCATTTAGGGTCGCAACGATGATGCTGACATGCATATGTATCTTGGCTGTTGACTTCACAATTTTTCCTAGAAGATATGCCAAGACTGAGACTTATGGTACTGGCTGGATGGATCTCGGGGTTGGCTCATTTGTCGTAGCAAACTCATTTGTGTCTCGGCAAGCACGAAATTTATCATGGACGAAATGGAAGACTGCAATTCAATCTACTAGTCCACTAATCATCCTTGGGTTTGTTCGTCTTCTCTCTACTGCAGGTCTAGACTATCAGCATCATGCAGCTGAATATGGAGTACACTGGAATTTCTTTTTCACACTTGCTGCCATATCATTTCTTACATCCATAATTAATGTGCCCGCGCAGTATTCTGGAATTCTCGGTGCACTAATTCTAGTAGGTTACGAAGGTTGGTTGATGCAGGGGTTAAACACATATCTGCTTTCTAATGAAAGGGGAAGTGATATAATTAGCCAAAATAAGGAGGGATTTTTTAGCATATTTGGATATTGGAGCATGTACCTCATTGGTGTCCAGGTGGGGAATTTTCTTTTTTTCAGTAACCACTCTGCTACAACAAGAAGCCATAAATGGACAAGGAATAGAGCTGCAATACTTTCACTTCTGTTTTGGCTAGTAACTGTGGTTATAGATAGCCATGTCCAGAGAGTTTCCCGTAGAATGTGCAATCTGGCTTATGTTACACTGGTATTGGCCGTAAATCTAGAGGGTATGGCCATAGTTCTGTTCTCTGATTGTTTCCCTGGAAGCAAAACATCAGTACTTGAAGAAGCATATAACCGCAATTTGCTAGCAATATTTCTTTTGGCAAATTTGCTTACTGGTCTGGTAAACTTTTTGGTGGATACCCTGTTTGCATCATCAGTCAAAGCTCTTTCTGTGATGATTGCCTATGCATTCACGTTAACTTTCATTCCTGGATTGCTAGACTTTTGCGGCATTCGATTTAGATTTTGGTGA

Protein Analysis

463

Amino Acids

52.01

Weight (kDa)

9.12

Isoelectric Point (pI)

31.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GWT1 PF06423 12 - 450 1e-130 GWT1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 659
AccII CGCG 1 cut(s) 773
AciI CCGC 5 cut(s) 93, 130, 771, 1213, 1368
AclWI GGATC 2 cut(s) 79, 534
AcoI YGGCCR 3 cut(s) 234, 1125, 1146
AcsI RAATTY 6 cut(s) 50, 583, 715, 786, 955, 1243
AcyI GRCGYC 1 cut(s) 37
AfaI GTAC 4 cut(s) 514, 708, 932, 1194
AfiI CCNNNNNNNGG 1 cut(s) 892
AgsI TTSAA 5 cut(s) 25, 148, 175, 617, 1199
AhlI ACTAGT 1 cut(s) 623
AjnI CCWGG 3 cut(s) 945, 1174, 1348
AluBI AGCT 4 cut(s) 116, 695, 1019, 1304
AluI AGCT 4 cut(s) 116, 695, 1019, 1304
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 3 cut(s) 44, 497, 573
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 2 cut(s) 79, 534
Ama87I CYCGRG 1 cut(s) 530
AoxI GGCC 3 cut(s) 234, 1125, 1146
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 5 cut(s) 127, 347, 692, 734, 1019
ApoI RAATTY 6 cut(s) 50, 583, 715, 786, 955, 1243
AseI ATTAAT 1 cut(s) 762
AspLEI GCGC 1 cut(s) 775
AsuHPI GGTGA 1 cut(s) 91
AsuNHI GCTAGC 1 cut(s) 1222
AvaI CYCGRG 1 cut(s) 530
BaeGI GKGCMC 2 cut(s) 771, 799
BalI TGGCCA 1 cut(s) 1148
BbsI GAAGAC 2 cut(s) 611, 647
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 5 cut(s) 114, 359, 704, 721, 1006
BccI CCATC 2 cut(s) 104, 517
BceAI ACGGC 2 cut(s) 221, 1112
BciT130I CCWGG 3 cut(s) 947, 1176, 1350
BciVI GTATCC 1 cut(s) 1270
BcoDI GTCTC 3 cut(s) 44, 497, 573
BcuI ACTAGT 1 cut(s) 623
BfaI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1046, 1136, 1223, 1358
BfmI CTRYAG 1 cut(s) 666
BfuAI ACCTGC 1 cut(s) 659
BfuI GTATCC 1 cut(s) 1270
BisI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1020, 1369
BlsI GCNGC 8 cut(s) 94, 129, 132, 349, 694, 736, 1021, 1370
BmcAI AGTACT 1 cut(s) 1194
Bme1390I CCNGG 3 cut(s) 947, 1176, 1350
BmeT110I CYCGRG 1 cut(s) 530
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 3 cut(s) 947, 1176, 1350
BmsI GCATC 5 cut(s) 178, 426, 694, 819, 1298
BmtI GCTAGC 1 cut(s) 1226
BpiI GAAGAC 2 cut(s) 611, 647
BpuEI CTTGAG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 237, 640, 1174
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse1I ACTGG 5 cut(s) 73, 520, 716, 1122, 1258
Bse3DI GCAATG 1 cut(s) 342
Bse8I GATNNNNATC 1 cut(s) 302
BseBI CCWGG 3 cut(s) 947, 1176, 1350
BseDI CCNNGG 3 cut(s) 237, 640, 1174
BseGI GGATG 4 cut(s) 303, 528, 636, 752
BseJI GATNNNNATC 1 cut(s) 302
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 5 cut(s) 73, 520, 716, 1122, 1258
BseSI GKGCMC 2 cut(s) 771, 799
BseXI GCAGC 5 cut(s) 114, 359, 704, 721, 1006
BseYI CCCAGC 1 cut(s) 112
Bsh1236I CGCG 1 cut(s) 773
BshFI GGCC 3 cut(s) 236, 1127, 1148
BsiHKAI GWGCWC 1 cut(s) 799
BsiHKCI CYCGRG 1 cut(s) 530
BslI CCNNNNNNNGG 1 cut(s) 892
BsmAI GTCTC 3 cut(s) 44, 497, 573
BsmBI CGTCTC 1 cut(s) 44
BsmI GAATGC 2 cut(s) 1328, 1371
BsnI GGCC 3 cut(s) 236, 1127, 1148
BsoBI CYCGRG 1 cut(s) 530
Bsp1286I GDGCHC 2 cut(s) 771, 799
Bsp143I GATC 2 cut(s) 71, 526
BspACI CCGC 5 cut(s) 93, 130, 771, 1213, 1368
BspANI GGCC 3 cut(s) 236, 1127, 1148
BspCNI CTCAG 1 cut(s) 493
BspFNI CGCG 1 cut(s) 773
BspLI GGNNCC 1 cut(s) 261
BspMAI CTGCAG 1 cut(s) 670
BspMI ACCTGC 1 cut(s) 659
BspOI GCTAGC 1 cut(s) 1226
BspPI GGATC 2 cut(s) 79, 534
BsrDI GCAATG 1 cut(s) 342
BsrI ACTGG 5 cut(s) 73, 520, 716, 1122, 1258
BssECI CCNNGG 3 cut(s) 237, 640, 1174
BssMI GATC 2 cut(s) 71, 526
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 640
Bst2UI CCWGG 3 cut(s) 947, 1176, 1350
Bst4CI ACNGT 2 cut(s) 283, 1054
BstACI GRCGYC 1 cut(s) 37
BstC8I GCNNGC 6 cut(s) 135, 216, 234, 576, 771, 1224
BstDEI CTNAG 2 cut(s) 204, 501
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 4 cut(s) 303, 528, 636, 752
BstFNI CGCG 1 cut(s) 773
BstHHI GCGC 1 cut(s) 775
BstKTI GATC 2 cut(s) 74, 529
BstMAI GTCTC 3 cut(s) 44, 497, 573
BstMBI GATC 2 cut(s) 71, 526
BstMWI GCNNNNNNNGC 2 cut(s) 224, 233
BstNI CCWGG 3 cut(s) 947, 1176, 1350
BstNSI RCATGY 2 cut(s) 447, 931
BstSCI CCNGG 3 cut(s) 945, 1174, 1348
BstSFI CTRYAG 1 cut(s) 666
BstSLI GKGCMC 2 cut(s) 771, 799
BstUI CGCG 1 cut(s) 773
BstV1I GCAGC 5 cut(s) 114, 359, 704, 721, 1006
BstV2I GAAGAC 2 cut(s) 611, 647
BstX2I RGATCY 2 cut(s) 71, 526
BstYI RGATCY 2 cut(s) 71, 526
BsuI GTATCC 1 cut(s) 1270
BsuRI GGCC 3 cut(s) 236, 1127, 1148
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 4 cut(s) 303, 528, 636, 752
BtsIMutI CAGTG 3 cut(s) 279, 709, 1115
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 6 cut(s) 135, 216, 234, 576, 771, 1224
CfoI GCGC 1 cut(s) 775
CseI GACGC 1 cut(s) 26
Csp6I GTAC 4 cut(s) 513, 707, 931, 1193
CviAII CATG 7 cut(s) 79, 223, 444, 591, 689, 928, 1070
CviQI GTAC 4 cut(s) 513, 707, 931, 1193
DdeI CTNAG 2 cut(s) 204, 501
DpnI GATC 2 cut(s) 73, 528
DpnII GATC 2 cut(s) 71, 526
EaeI YGGCCR 3 cut(s) 234, 1125, 1146
Eco130I CCWWGG 1 cut(s) 640
Eco88I CYCGRG 1 cut(s) 530
EcoRI GAATTC 2 cut(s) 50, 786
EcoRII CCWGG 3 cut(s) 945, 1174, 1348
EcoT14I CCWWGG 1 cut(s) 640
EcoT22I ATGCAT 2 cut(s) 449, 1330
ErhI CCWWGG 1 cut(s) 640
Esp3I CGTCTC 1 cut(s) 44
FaeI CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1073
FatI CATG 7 cut(s) 78, 222, 443, 590, 688, 927, 1069
FauI CCCGC 1 cut(s) 778
FauNDI CATATG 1 cut(s) 449
Fnu4HI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1020, 1369
FokI GGATG 4 cut(s) 310, 535, 623, 739
Fsp4HI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1020, 1369
FspBI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1046, 1136, 1223, 1358
GlaI GCGC 1 cut(s) 774
GluI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1020, 1369
GsaI CCCAGC 1 cut(s) 116
HaeIII GGCC 3 cut(s) 236, 1127, 1148
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 775
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1073
Hin6I GCGC 1 cut(s) 773
HinP1I GCGC 1 cut(s) 773
HincII GTYRAC 2 cut(s) 466, 1338
HindII GTYRAC 2 cut(s) 466, 1338
HinfI GANTC 2 cut(s) 5, 392
HpaI GTTAAC 1 cut(s) 1338
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 6 cut(s) 466, 629, 709, 797, 1264, 1338
Hpy188I TCNGA 2 cut(s) 292, 1165
Hpy188III TCNNGA 4 cut(s) 674, 783, 1075, 1136
Hpy8I GTNNAC 6 cut(s) 466, 629, 709, 797, 1264, 1338
HpyAV CCTTC 1 cut(s) 812
HpyCH4III ACNGT 2 cut(s) 283, 1054
HpyCH4IV ACGT 1 cut(s) 1334
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 233
HpyF3I CTNAG 2 cut(s) 204, 501
HpySE526I ACGT 1 cut(s) 1334
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1073
HspAI GCGC 1 cut(s) 773
KspAI GTTAAC 1 cut(s) 1338
Kzo9I GATC 2 cut(s) 71, 526
LmnI GCTCC 1 cut(s) 924
Lsp1109I GCAGC 5 cut(s) 114, 359, 704, 721, 1006
LweI GCATC 5 cut(s) 178, 426, 694, 819, 1298
MaeI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1046, 1136, 1223, 1358
MaeII ACGT 1 cut(s) 1334
MaeIII GTNAC 6 cut(s) 166, 327, 812, 971, 1048, 1111
MalI GATC 2 cut(s) 73, 528
MboI GATC 2 cut(s) 71, 526
MboII GAAGA 9 cut(s) 59, 66, 187, 392, 395, 498, 616, 647, 1211
MflI RGATCY 2 cut(s) 71, 526
MhlI GDGCHC 2 cut(s) 771, 799
MlsI TGGCCA 1 cut(s) 1148
MluNI TGGCCA 1 cut(s) 1148
MnlI CCTC 3 cut(s) 889, 944, 1132
Mox20I TGGCCA 1 cut(s) 1148
Mph1103I ATGCAT 2 cut(s) 449, 1330
MscI TGGCCA 1 cut(s) 1148
MseI TTAA 4 cut(s) 272, 762, 839, 1337
MslI CAYNNNNRTG 1 cut(s) 448
Msp20I TGGCCA 1 cut(s) 1148
MspA1I CMGCKG 1 cut(s) 695
MspR9I CCNGG 3 cut(s) 947, 1176, 1350
Mva1269I GAATGC 2 cut(s) 1328, 1371
MvaI CCWGG 3 cut(s) 947, 1176, 1350
MvnI CGCG 1 cut(s) 773
MwoI GCNNNNNNNGC 2 cut(s) 224, 233
NdeI CATATG 1 cut(s) 449
NdeII GATC 2 cut(s) 71, 526
NheI GCTAGC 1 cut(s) 1222
NlaIII CATG 7 cut(s) 82, 226, 447, 594, 692, 931, 1073
NlaIV GGNNCC 1 cut(s) 261
NmeAIII GCCGAG 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 2 cut(s) 449, 1330
NspI RCATGY 2 cut(s) 447, 931
PcsI WCGNNNNNNNCGW 1 cut(s) 577
PctI GAATGC 2 cut(s) 1328, 1371
PfeI GAWTC 2 cut(s) 5, 392
PfoI TCCNGGA 1 cut(s) 1348
PkrI GCNGC 8 cut(s) 94, 129, 132, 349, 694, 736, 1021, 1370
PshBI ATTAAT 1 cut(s) 762
Psp6I CCWGG 3 cut(s) 945, 1174, 1348
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 3 cut(s) 945, 1174, 1348
PspN4I GGNNCC 1 cut(s) 261
PstI CTGCAG 1 cut(s) 670
PsuI RGATCY 2 cut(s) 71, 526
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 4 cut(s) 514, 708, 932, 1194
RsaNI GTAC 4 cut(s) 513, 707, 931, 1193
RseI CAYNNNNRTG 1 cut(s) 448
SaqAI TTAA 4 cut(s) 272, 762, 839, 1337
SatI GCNGC 8 cut(s) 93, 128, 131, 348, 693, 735, 1020, 1369
Sau3AI GATC 2 cut(s) 71, 526
ScaI AGTACT 1 cut(s) 1194
ScrFI CCNGG 3 cut(s) 947, 1176, 1350
SduI GDGCHC 2 cut(s) 771, 799
SfaNI GCATC 5 cut(s) 178, 426, 694, 819, 1298
SfcI CTRYAG 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 448
SmlI CTYRAG 1 cut(s) 41
SmoI CTYRAG 1 cut(s) 41
SpeI ACTAGT 1 cut(s) 623
SsiI CCGC 5 cut(s) 93, 130, 771, 1213, 1368
SspI AATATT 1 cut(s) 1230
SspMI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1046, 1136, 1223, 1358
StyD4I CCNGG 3 cut(s) 945, 1174, 1348
StyI CCWWGG 1 cut(s) 640
TaaI ACNGT 2 cut(s) 283, 1054
TaiI ACGT 1 cut(s) 1337
TaqI TCGA 1 cut(s) 1375
TatI WGTACW 2 cut(s) 706, 1192
TauI GCSGC 3 cut(s) 95, 133, 1371
TfiI GAWTC 2 cut(s) 5, 392
Tru1I TTAA 4 cut(s) 272, 762, 839, 1337
Tru9I TTAA 4 cut(s) 272, 762, 839, 1337
TscAI CASTG 3 cut(s) 286, 716, 1122
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 5 cut(s) 127, 347, 692, 734, 1019
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 3 cut(s) 241, 876, 1333
TspRI CASTG 3 cut(s) 286, 716, 1122
VneI GTGCAC 1 cut(s) 795
VspI ATTAAT 1 cut(s) 762
XapI RAATTY 6 cut(s) 50, 583, 715, 786, 955, 1243
XbaI TCTAGA 2 cut(s) 673, 1135
XceI RCATGY 2 cut(s) 447, 931
XspI CTAG 9 cut(s) 228, 483, 624, 674, 806, 1046, 1136, 1223, 1358
ZrmI AGTACT 1 cut(s) 1194
Zsp2I ATGCAT 2 cut(s) 449, 1330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.