Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

OG0010083

Physical Location & Seq

Genomic Coordinates Forward (+)

5090068 .. 5091861

Gene Structure 1794 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom02g07300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGAAGACCCAGGTTCCCAATCCGAAATCCAATCCGATTTCCGGCCGACCCGTGAGAAAATCCGGCCAATCAACCGGAACCCAGAACAGGATTTTCATTGAAAACATCTACGAGAGCAGAGCTGAGCCGCGTGATGGAGCGGCGGTGCAGATGACCCTCCTGTTCTTCACAGCCCGTCCGACCTTCTGGGTTCGAGCCTCCAAGATCGCCATTTCTCATTTCCACAGCCTCGCCCACGGCGGAGCTCGGCCGCGCCCGCTTTACGACTTCGAGGTAACGTCAAACCCAGAGGTTTGGTTTGTAAAAGTTGTTTGCACTCTGTTTCTTCGCTCGCATTCATTGGATTTGGGTTACCTTAGTAAGAACCTTTCGCCTTCGATTGCTTTTGAGGTTATTAAGCGGTTGAACAATCCGAAATTGGGGTTGAAATTCTTTGAGCTCAGTAGGGTTAGTCTGAGTGTTAAGCATAGTGTATGGACTTACAATTTTCTTTTGAGGTCTCTGTGTCAAATGGGGCTTCAAGATTCTGCTAAATTGGTGTTTGATTACATGAGGAGTGACGGGCATACGCCAGATGATTCAGTTGTAGAACTCTTGGTGTCGTCGTATGCGCAGATAGGCAAGTTGGATGATGCTGAGAAGTTTCTTGATGAGGCTCATTGTGGTGAGATTAGATTGCCACCTTTCGTATACAATAACTTGTTCAATGTGTTGGTTAAACAGAATAAAGTAGATGAGGCTGTTAGCTTGTTTAGAAAGCATATGGGGCCGCATTGTCACCCGGATAACTGGACTTTCAATATTCTAATTCGAGGTTTATGCAGAGTTGGAGAAATTGACAAGGCTTTTGAGTTGTTCAGTGATATGGGGAGTTCTGGTTGCTACCCTGATATAGTTACATATAACACACTAATTAGTGGACTTTGTAGGGCTAATGAGGTAGATAGGGGATTTCAATTGCTTAAAGAGGTTCAATCAAGAATCGAACTTTCACCTGATGTTATAACTTTTACATCAGTCATATCAGGATATTGCAAGTTGGGTAAGATGGAGGAGGCCTCCGTTCTTTTTGATGAGATGATTAGTGCCGGAGTTAGACCAACTTCTGTTACTTTCAATGCAATGATTGATGGATTTGGTAAGACTGGCAACATGGGTTCTGCACTTGCTATGCATCAGAAAATGCTCTTTCATGGTTATCATTCAGACGTTTTTACCTTCACTTCCCTAATTGATGGCCATTGTCGAGCTGGGCAACTGAGTCAGGGATTAAAGCTTTGGCAGGAGATGAATGGGAAAAATGTGTCTCCAAGTGCGTACACTTTCTCTATCCTTATTAATGCTTTGTGCAAGGAGAGTAGACTACATGAAGCACGTGATTTCCTGAGGCAATTAAAGTGGAGTAATGTGGTTCCGAAACCTTTTATATATAACCCTGTGATAGATGGATTCTGTAAGGCTGGCAATGTTGATCAGGCAAACGCCATTGTGGCAGAGATGGAAGAGAAGAGATGCAGCCCTGATAAAGTGACATTTACCATTCTCATACTCGGGAATTGTATGAAAGGGAGAATGTCTGAGGCAATTAGCAATTTTAAAAAGATGTTGGCAATTGGTTGTGCCCCAGACAACATCACCGTAAATTCTTTGACATCTTGCCTCATTAAGGCTGGGATGGCTAATGAAGCCCATCACATTAAGCAAATTGCATATAATGACCTCAATTCGGGCATGTCACCTACAGGAAGAACTGATCATATGAAAGCAAATGCACAAATTCCAGTGGCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

598

Amino Acids

66.57

Weight (kDa)

8.67

Isoelectric Point (pI)

37.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	200 - 323	1.9e-06	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	229 - 275	7.3e-11	PPR repeat family
PPR_1	PF12854	259 - 289	9.2e-13	PPR repeat
PPR_3	PF13812	261 - 306	3.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	261 - 310	5.2e-19	PPR repeat family
PPR	PF01535	265 - 294	2.7e-08	PPR repeat
PPR_1	PF12854	293 - 323	4e-13	PPR repeat
PPR_2	PF13041	296 - 325	4.5e-07	PPR repeat family
PPR_3	PF13812	321 - 381	4.5e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	330 - 360	2.3e-12	PPR repeat
PPR_2	PF13041	332 - 381	5e-17	PPR repeat family
PPR	PF01535	335 - 365	8.8e-10	PPR repeat
PPR_1	PF12854	364 - 395	6.3e-06	PPR repeat
PPR_2	PF13041	367 - 416	1.8e-12	PPR repeat family
PPR	PF01535	370 - 399	9.3e-06	PPR repeat
PPR_1	PF12854	399 - 430	1e-08	PPR repeat
PPR_2	PF13041	403 - 451	5.7e-17	PPR repeat family
PPR	PF01535	405 - 434	3.7e-06	PPR repeat
PPR_long	PF17177	417 - 561	7.8e-09	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	433 - 464	2.5e-08	PPR repeat
PPR_1	PF12854	470 - 501	2.3e-07	PPR repeat
PPR_3	PF13812	474 - 516	7.1e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	475 - 520	1.3e-13	PPR repeat family
PPR_1	PF12854	503 - 535	1.1e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010083)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G06000 AT2G06000
fragaria_vesca	FvH4_5g31880
malus_domestica	MD02G1091700.v1.1 MD15G1217800.v1.1
prunus_persica	Prupe.7G198600_v2.0.a1
pyrus_communis	pycom02g07300 pycom15g19340 pycom15g33430 pycom15g33440
rosa_chinensis	RchiOBHm_Chr7g0227901
rosa_laevigata	RLG00000001605
rosa_multiflora	Rmu_co8402659.1_g000001 Rmu_sc0000347.1_g000003
rosa_roxburghii	Rroxscaffold_3G00231780
rosa_rugosa	Rorug07G0244100
rosa_samantha	Rh7AG397500 Rh7BG379100 Rh7CG415700 Rh7DG393000
rosa_wichuraiana	Rw7G033070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1004
Acc16I	TGCGCA	1 cut(s)	612
AccBSI	CCGCTC	1 cut(s)	140
AccI	GTMKAC	2 cut(s)	690, 1360
AccII	CGCG	2 cut(s)	130, 253
AciI	CCGC	8 cut(s)	128, 140, 143, 240, 251, 257, 400, 770
AcoI	YGGCCR	4 cut(s)	43, 64, 248, 1237
AcsI	RAATTY	3 cut(s)	428, 1642, 1776
AcvI	CACGTG	1 cut(s)	1376
AfaI	GTAC	1 cut(s)	1319
AfiI	CCNNNNNNNGG	6 cut(s)	41, 87, 134, 419, 1666, 1726
AgsI	TTSAA	9 cut(s)	101, 406, 427, 521, 706, 799, 956, 974, 1117
AjnI	CCWGG	1 cut(s)	9
AluBI	AGCT	6 cut(s)	122, 245, 439, 747, 1250, 1276
AluI	AGCT	6 cut(s)	122, 245, 439, 747, 1250, 1276
Alw21I	GWGCWC	2 cut(s)	247, 441
Alw26I	GTCTC	2 cut(s)	504, 1311
AlwNI	CAGNNNCTG	1 cut(s)	1787
Ama87I	CYCGRG	1 cut(s)	1550
AoxI	GGCC	6 cut(s)	43, 64, 248, 767, 1056, 1237
ApeKI	GCWGC	1 cut(s)	1515
ApoI	RAATTY	3 cut(s)	428, 1642, 1776
ArsI	GACNNNNNNTTYG	2 cut(s)	830, 862
AseI	ATTAAT	1 cut(s)	1338
AspLEI	GCGC	2 cut(s)	255, 613
AspS9I	GGNCC	1 cut(s)	767
AsuC2I	CCSGG	1 cut(s)	782
AsuHPI	GGTGA	5 cut(s)	677, 770, 984, 1627, 1728
AvaI	CYCGRG	1 cut(s)	1550
AxyI	CCTNAGG	1 cut(s)	1385
BaeGI	GKGCMC	1 cut(s)	1624
BalI	TGGCCA	1 cut(s)	1239
BanII	GRGCYC	2 cut(s)	247, 441
BbrPI	CACGTG	1 cut(s)	1376
BbsI	GAAGAC	1 cut(s)	11
Bbv12I	GWGCWC	2 cut(s)	247, 441
BbvI	GCAGC	1 cut(s)	1527
BccI	CCATC	8 cut(s)	128, 1042, 1124, 1229, 1439, 1492, 1669, 1698
BceAI	ACGGC	1 cut(s)	253
BcgI	CGANNNNNNTGC	2 cut(s)	801, 835
BciT130I	CCWGG	1 cut(s)	11
BclI	TGATCA	2 cut(s)	1471, 1753
BcnI	CCSGG	1 cut(s)	782
BcoDI	GTCTC	2 cut(s)	504, 1311
BfmI	CTRYAG	1 cut(s)	1740
BglI	GCCNNNNNGGC	1 cut(s)	1490
BisI	GCNGC	5 cut(s)	128, 141, 251, 770, 1516
BlpI	GCTNAGC	1 cut(s)	123
BlsI	GCNGC	5 cut(s)	129, 142, 252, 771, 1517
Bme1390I	CCNGG	2 cut(s)	11, 782
BmeT110I	CYCGRG	1 cut(s)	1550
BmgT120I	GGNCC	1 cut(s)	767
BmiI	GGNNCC	4 cut(s)	15, 79, 768, 1413
BmrFI	CCNGG	2 cut(s)	11, 782
BmsI	GCATC	3 cut(s)	622, 1183, 1502
BpiI	GAAGAC	1 cut(s)	11
BplI	GAGNNNNNCTC	2 cut(s)	1043, 1075
Bpu1102I	GCTNAGC	1 cut(s)	123
BpuMI	CCSGG	1 cut(s)	782
BsaAI	YACGTR	1 cut(s)	1376
BsaI	GGTCTC	1 cut(s)	504
BsaJI	CCNNGG	2 cut(s)	9, 235
BsaWI	WCCGGW	1 cut(s)	74
BsaXI	ACNNNNNCTCC	4 cut(s)	129, 159, 862, 892
Bsc4I	CCNNNNNNNGG	6 cut(s)	41, 87, 134, 419, 1666, 1726
Bse1I	ACTGG	3 cut(s)	794, 1150, 1781
Bse21I	CCTNAGG	1 cut(s)	1385
Bse3DI	GCAATG	2 cut(s)	1128, 1471
BseBI	CCWGG	1 cut(s)	11
BseDI	CCNNGG	2 cut(s)	9, 235
BseGI	GGATG	2 cut(s)	634, 1680
BseLI	CCNNNNNNNGG	6 cut(s)	41, 87, 134, 419, 1666, 1726
BseMI	GCAATG	2 cut(s)	1128, 1471
BseMII	CTCAG	7 cut(s)	114, 446, 454, 627, 1250, 1376, 1569
BseNI	ACTGG	3 cut(s)	794, 1150, 1781
BseRI	GAGGAG	2 cut(s)	568, 1067
BseSI	GKGCMC	1 cut(s)	1624
BseX3I	CGGCCG	2 cut(s)	43, 248
BseXI	GCAGC	1 cut(s)	1527
BseYI	CCCAGC	2 cut(s)	1250, 1670
BsgI	GTGCAG	2 cut(s)	167, 1146
Bsh1236I	CGCG	2 cut(s)	130, 253
Bsh1285I	CGRYCG	2 cut(s)	46, 251
BshFI	GGCC	6 cut(s)	45, 66, 250, 769, 1058, 1239
BsiEI	CGRYCG	2 cut(s)	46, 251
BsiHKAI	GWGCWC	2 cut(s)	247, 441
BsiHKCI	CYCGRG	1 cut(s)	1550
BsiSI	CCGG	5 cut(s)	42, 63, 75, 782, 1089
BslI	CCNNNNNNNGG	6 cut(s)	41, 87, 134, 419, 1666, 1726
BsmAI	GTCTC	2 cut(s)	504, 1311
BsmI	GAATGC	1 cut(s)	334
BsnI	GGCC	6 cut(s)	45, 66, 250, 769, 1058, 1239
Bso31I	GGTCTC	1 cut(s)	504
BsoBI	CYCGRG	1 cut(s)	1550
Bsp1286I	GDGCHC	3 cut(s)	247, 441, 1624
Bsp143I	GATC	3 cut(s)	204, 1471, 1753
Bsp1720I	GCTNAGC	1 cut(s)	123
BspACI	CCGC	8 cut(s)	128, 140, 143, 240, 251, 257, 400, 770
BspANI	GGCC	6 cut(s)	45, 66, 250, 769, 1058, 1239
BspCNI	CTCAG	7 cut(s)	115, 447, 453, 628, 1251, 1377, 1570
BspFNI	CGCG	2 cut(s)	130, 253
BspLI	GGNNCC	4 cut(s)	15, 79, 768, 1413
BspTNI	GGTCTC	1 cut(s)	504
BsrBI	CCGCTC	1 cut(s)	140
BsrDI	GCAATG	2 cut(s)	1128, 1471
BsrI	ACTGG	3 cut(s)	794, 1150, 1781
BssECI	CCNNGG	2 cut(s)	9, 235
BssMI	GATC	3 cut(s)	204, 1471, 1753
BssNAI	GTATAC	1 cut(s)	691
Bst1107I	GTATAC	1 cut(s)	691
Bst2UI	CCWGG	1 cut(s)	11
Bst4CI	ACNGT	1 cut(s)	1639
Bst6I	CTCTTC	2 cut(s)	1497, 1502
BstBAI	YACGTR	1 cut(s)	1376
BstC8I	GCNNGC	3 cut(s)	257, 332, 1462
BstDEI	CTNAG	8 cut(s)	123, 356, 440, 455, 636, 1259, 1385, 1578
BstDSI	CCRYGG	1 cut(s)	235
BstEII	GGTNACC	1 cut(s)	350
BstENI	CCTNNNNNAGG	1 cut(s)	1664
BstF5I	GGATG	2 cut(s)	634, 1680
BstFNI	CGCG	2 cut(s)	130, 253
BstHHI	GCGC	2 cut(s)	255, 613
BstKTI	GATC	3 cut(s)	207, 1474, 1756
BstMAI	GTCTC	2 cut(s)	504, 1311
BstMBI	GATC	3 cut(s)	204, 1471, 1753
BstMCI	CGRYCG	2 cut(s)	46, 251
BstMWI	GCNNNNNNNGC	5 cut(s)	256, 766, 1490, 1676, 1685
BstNI	CCWGG	1 cut(s)	11
BstNSI	RCATGY	1 cut(s)	1735
BstPI	GGTNACC	1 cut(s)	350
BstSCI	CCNGG	2 cut(s)	9, 780
BstSFI	CTRYAG	1 cut(s)	1740
BstSLI	GKGCMC	1 cut(s)	1624
BstUI	CGCG	2 cut(s)	130, 253
BstV1I	GCAGC	1 cut(s)	1527
BstV2I	GAAGAC	1 cut(s)	11
BstXI	CCANNNNNNTGG	1 cut(s)	294
BstZ17I	GTATAC	1 cut(s)	691
BstZI	CGGCCG	2 cut(s)	43, 248
Bsu36I	CCTNAGG	1 cut(s)	1385
BsuRI	GGCC	6 cut(s)	45, 66, 250, 769, 1058, 1239
BtgI	CCRYGG	1 cut(s)	235
BtsCI	GGATG	2 cut(s)	634, 1680
BtsIMutI	CAGTG	2 cut(s)	865, 1788
Cac8I	GCNNGC	3 cut(s)	257, 332, 1462
CaiI	CAGNNNCTG	1 cut(s)	1787
CfoI	GCGC	2 cut(s)	255, 613
Cfr13I	GGNCC	1 cut(s)	767
Csp6I	GTAC	1 cut(s)	1318
CspCI	CAANNNNNGTGG	2 cut(s)	1379, 1414
CviAII	CATG	5 cut(s)	550, 1153, 1193, 1367, 1732
CviQI	GTAC	1 cut(s)	1318
DdeI	CTNAG	8 cut(s)	123, 356, 440, 455, 636, 1259, 1385, 1578
DpnI	GATC	3 cut(s)	206, 1473, 1755
DpnII	GATC	3 cut(s)	204, 1471, 1753
DraI	TTTAAA	1 cut(s)	1597
EaeI	YGGCCR	4 cut(s)	43, 64, 248, 1237
EagI	CGGCCG	2 cut(s)	43, 248
Eam1104I	CTCTTC	2 cut(s)	1497, 1502
EarI	CTCTTC	2 cut(s)	1497, 1502
EciI	GGCGGA	1 cut(s)	255
Ecl136II	GAGCTC	2 cut(s)	245, 439
EclXI	CGGCCG	2 cut(s)	43, 248
Eco147I	AGGCCT	1 cut(s)	1058
Eco24I	GRGCYC	2 cut(s)	247, 441
Eco31I	GGTCTC	1 cut(s)	504
Eco52I	CGGCCG	2 cut(s)	43, 248
Eco53kI	GAGCTC	2 cut(s)	245, 439
Eco72I	CACGTG	1 cut(s)	1376
Eco81I	CCTNAGG	1 cut(s)	1385
Eco88I	CYCGRG	1 cut(s)	1550
Eco91I	GGTNACC	1 cut(s)	350
EcoICRI	GAGCTC	2 cut(s)	245, 439
EcoNI	CCTNNNNNAGG	1 cut(s)	1664
EcoO65I	GGTNACC	1 cut(s)	350
EcoRII	CCWGG	1 cut(s)	9
EcoT22I	ATGCAT	1 cut(s)	1176
EcoT38I	GRGCYC	2 cut(s)	247, 441
FaeI	CATG	5 cut(s)	553, 1156, 1196, 1370, 1735
FatI	CATG	5 cut(s)	549, 1152, 1192, 1366, 1731
FauI	CCCGC	1 cut(s)	264
FauNDI	CATATG	2 cut(s)	762, 1758
FbaI	TGATCA	2 cut(s)	1471, 1753
FblI	GTMKAC	2 cut(s)	690, 1360
Fnu4HI	GCNGC	5 cut(s)	128, 141, 251, 770, 1516
FokI	GGATG	2 cut(s)	641, 1687
FriOI	GRGCYC	2 cut(s)	247, 441
Fsp4HI	GCNGC	5 cut(s)	128, 141, 251, 770, 1516
FspI	TGCGCA	1 cut(s)	612
GlaI	GCGC	2 cut(s)	254, 612
GluI	GCNGC	5 cut(s)	128, 141, 251, 770, 1516
GsaI	CCCAGC	2 cut(s)	1254, 1674
HaeIII	GGCC	6 cut(s)	45, 66, 250, 769, 1058, 1239
HapII	CCGG	5 cut(s)	42, 63, 75, 782, 1089
HhaI	GCGC	2 cut(s)	255, 613
Hin1II	CATG	5 cut(s)	553, 1156, 1196, 1370, 1735
Hin6I	GCGC	2 cut(s)	253, 611
HinP1I	GCGC	2 cut(s)	253, 611
HindIII	AAGCTT	1 cut(s)	1274
HinfI	GANTC	5 cut(s)	524, 578, 981, 1261, 1449
HpaII	CCGG	5 cut(s)	42, 63, 75, 782, 1089
HphI	GGTGA	5 cut(s)	677, 770, 984, 1627, 1728
Hpy166II	GTNNAC	4 cut(s)	691, 920, 1320, 1361
Hpy188I	TCNGA	9 cut(s)	24, 36, 180, 414, 456, 1179, 1207, 1416, 1579
Hpy188III	TCNNGA	6 cut(s)	521, 647, 978, 1026, 1384, 1552
Hpy8I	GTNNAC	4 cut(s)	691, 920, 1320, 1361
Hpy99I	CGWCG	1 cut(s)	607
HpyAV	CCTTC	3 cut(s)	193, 384, 1228
HpyCH4III	ACNGT	1 cut(s)	1639
HpyCH4IV	ACGT	3 cut(s)	278, 1209, 1375
HpyF10VI	GCNNNNNNNGC	5 cut(s)	256, 766, 1490, 1676, 1685
HpyF3I	CTNAG	8 cut(s)	123, 356, 440, 455, 636, 1259, 1385, 1578
HpySE526I	ACGT	3 cut(s)	278, 1209, 1375
Hsp92II	CATG	5 cut(s)	553, 1156, 1196, 1370, 1735
HspAI	GCGC	2 cut(s)	253, 611
Ksp22I	TGATCA	2 cut(s)	1471, 1753
Kzo9I	GATC	3 cut(s)	204, 1471, 1753
LmnI	GCTCC	2 cut(s)	137, 242
Lsp1109I	GCAGC	1 cut(s)	1527
LweI	GCATC	3 cut(s)	622, 1183, 1502
MaeII	ACGT	3 cut(s)	278, 1209, 1375
MaeIII	GTNAC	8 cut(s)	274, 350, 557, 776, 895, 1108, 1528, 1734
MalI	GATC	3 cut(s)	206, 1473, 1755
MbiI	CCGCTC	1 cut(s)	140
MboI	GATC	3 cut(s)	204, 1471, 1753
MboII	GAAGA	6 cut(s)	16, 157, 317, 1514, 1519, 1758
MfeI	CAATTG	2 cut(s)	956, 1611
MhlI	GDGCHC	3 cut(s)	247, 441, 1624
MlsI	TGGCCA	1 cut(s)	1239
MluNI	TGGCCA	1 cut(s)	1239
MlyI	GAGTC	1 cut(s)	1270
MmeI	TCCRAC	3 cut(s)	203, 606, 808
Mox20I	TGGCCA	1 cut(s)	1239
Mph1103I	ATGCAT	1 cut(s)	1176
MscI	TGGCCA	1 cut(s)	1239
MslI	CAYNNNNRTG	1 cut(s)	663
Msp20I	TGGCCA	1 cut(s)	1239
MspI	CCGG	5 cut(s)	42, 63, 75, 782, 1089
MspR9I	CCNGG	2 cut(s)	11, 782
MunI	CAATTG	2 cut(s)	956, 1611
Mva1269I	GAATGC	1 cut(s)	334
MvaI	CCWGG	1 cut(s)	11
MvnI	CGCG	2 cut(s)	130, 253
MwoI	GCNNNNNNNGC	5 cut(s)	256, 766, 1490, 1676, 1685
NciI	CCSGG	1 cut(s)	782
NdeI	CATATG	2 cut(s)	762, 1758
NdeII	GATC	3 cut(s)	204, 1471, 1753
NlaIII	CATG	5 cut(s)	553, 1156, 1196, 1370, 1735
NlaIV	GGNNCC	4 cut(s)	15, 79, 768, 1413
NmeAIII	GCCGAG	1 cut(s)	226
NmuCI	GTSAC	4 cut(s)	557, 776, 1528, 1734
NsbI	TGCGCA	1 cut(s)	612
NsiI	ATGCAT	1 cut(s)	1176
NspI	RCATGY	1 cut(s)	1735
PceI	AGGCCT	1 cut(s)	1058
PctI	GAATGC	1 cut(s)	334
PfeI	GAWTC	4 cut(s)	524, 578, 981, 1449
PkrI	GCNGC	5 cut(s)	129, 142, 252, 771, 1517
PleI	GAGTC	1 cut(s)	1269
PmaCI	CACGTG	1 cut(s)	1376
PmlI	CACGTG	1 cut(s)	1376
PpsI	GAGTC	1 cut(s)	1269
Ppu21I	YACGTR	1 cut(s)	1376
PshBI	ATTAAT	1 cut(s)	1338
PsiI	TTATAA	1 cut(s)	1004
Psp124BI	GAGCTC	2 cut(s)	247, 441
Psp6I	CCWGG	1 cut(s)	9
PspCI	CACGTG	1 cut(s)	1376
PspEI	GGTNACC	1 cut(s)	350
PspFI	CCCAGC	2 cut(s)	1250, 1670
PspGI	CCWGG	1 cut(s)	9
PspN4I	GGNNCC	4 cut(s)	15, 79, 768, 1413
PspPI	GGNCC	1 cut(s)	767
PstNI	CAGNNNCTG	1 cut(s)	1787
RsaI	GTAC	1 cut(s)	1319
RsaNI	GTAC	1 cut(s)	1318
RseI	CAYNNNNRTG	1 cut(s)	663
SacI	GAGCTC	2 cut(s)	247, 441
SatI	GCNGC	5 cut(s)	128, 141, 251, 770, 1516
Sau3AI	GATC	3 cut(s)	204, 1471, 1753
Sau96I	GGNCC	1 cut(s)	767
SchI	GAGTC	1 cut(s)	1270
ScrFI	CCNGG	2 cut(s)	11, 782
SduI	GDGCHC	3 cut(s)	247, 441, 1624
SfaNI	GCATC	3 cut(s)	622, 1183, 1502
SfcI	CTRYAG	1 cut(s)	1740
SmiMI	CAYNNNNRTG	1 cut(s)	663
SseBI	AGGCCT	1 cut(s)	1058
SsiI	CCGC	8 cut(s)	128, 140, 143, 240, 251, 257, 400, 770
SspI	AATATT	1 cut(s)	802
SstI	GAGCTC	2 cut(s)	247, 441
StuI	AGGCCT	1 cut(s)	1058
StyD4I	CCNGG	2 cut(s)	9, 780
TaaI	ACNGT	1 cut(s)	1639
TaiI	ACGT	3 cut(s)	281, 1212, 1378
TaqI	TCGA	6 cut(s)	193, 270, 377, 811, 984, 1246
TauI	GCSGC	4 cut(s)	130, 143, 253, 772
TfiI	GAWTC	4 cut(s)	524, 578, 981, 1449
TscAI	CASTG	2 cut(s)	865, 1788
TseFI	GTSAC	4 cut(s)	557, 776, 1528, 1734
TseI	GCWGC	1 cut(s)	1515
Tsp45I	GTSAC	4 cut(s)	557, 776, 1528, 1734
TspDTI	ATGAA	9 cut(s)	17, 85, 327, 1181, 1304, 1383, 1577, 1698, 1775
TspGWI	ACGGA	1 cut(s)	1051
TspRI	CASTG	2 cut(s)	865, 1788
VspI	ATTAAT	1 cut(s)	1338
XagI	CCTNNNNNAGG	1 cut(s)	1664
XapI	RAATTY	3 cut(s)	428, 1642, 1776
XceI	RCATGY	1 cut(s)	1735
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1247
XmiI	GTMKAC	2 cut(s)	690, 1360
Zsp2I	ATGCAT	1 cut(s)	1176

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.