Prupe.8G196400_v2.0.a1
ERF Family

P-loop containing nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0006980
Physical Location & Seq
Forward (+)
18806900 .. 18810061
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G196400.1

Sequence Viewer

Length: 1620 bp
ATGGGTGGCGATATAACAACTCCTCCTGGCAGTGATACAGGGGAAGACTTTTCGCTGGAGCAAGATTCTTCTCAGCTCTCATCTCCACTTCTCAGAAATGATTTGAGCATGAATGTCAAGGATGCTGCAGAAGGTACACTAAATGGGTCGGTGCTTGGAGAACTTGACAGTGTGGATTCTCGATTTTCTTCGGCTTCTTTGGATTTTGAAGTCAAACAAAAGCGGAGGGAAAGTGTTTATAGAGAAATTTTGCTGAGTAATGACGGACTGCGGATTCGGGGCAAAGGTTTGGAAGAAGCCAAGATTAAAGTATTGAGCTACACTCCTGGTGCATGGATTGAAAGTGTTGGTGCTGTGAAATTGAGGGACTATGATGTGCCAAAGACGACAACTGTTTTATTGGTTGGTCCAAAAGGATCTGGAAAGAGTAGTCTTGTAAATAGAATATCAAAGGTGTTTGAAGATGACAAGTCTGCGTCAGAAAGAGCCCAAGTATCATATAATTCATCTGTTGGAGATGGAACCTTTTTCCTCCAGGAATATATGATACCGAGATGTTCCACTTCTTTCTGCCTCTATGATACCCGTAGTTTGTGTGATGATTCACATGAAAACATAAAAATACTGCAGCATTGGATGAAAAATGGTGTTCGTCAAGGGGAGCTTGTTATCAGGGATTCAGACAGCCAAAGTTTGAGGACCATGATGATGTGCAAAGCTCGTGATGATGGTTATCTGTCCAGTGAGATCAGGAAGGTTAATTTTGTCATATTTGTTGTTAATGGCCTTTCAGTTCTGAAAGCGATAGAAAGTGATGAAGATGCAGAGACTCGATACACCCAAATGATTGCTTCAGCTTTCAACTGCCCATACCTGGCATTTAAAGATGATAAACCCCTAGTTGTTGTCACGCATGGTGATCTACTTTCACCTGTTCAACGTGCTCGTGTTCGTGTCCATTTGGGAGAACTACTGGGTATTCCGCCTGCAACTCAAGTTTTTGACATCCCAGAAAGCAGTGATCCGGTAACTGAGTTGGCAATAGTTGACATGTTACGCTATTCGCTTGAGCGTGCTGAGAAAAATCTTCCCCATAAAAGAAAGGTTCCTACTATGTCACTGTTATCAGGTATGCTCCTGTTAGTAATCCTTGGAATTGCCATCAATATATGGTGTATGAATTATCAAGCGTGTATTCAGCAAGGCCCTTTCCCTTCGTCCCCAGAGGACATTCGGCATTGCCCTTCGCCTCAAATGAATCTTCAGCAGGGCCATGGTTCTTCTCCCCAAGCGCATGTTCAGCATGGCCCTTTGCCTGAAGTACATATTCAGCATGGCCCTCCTCCCCCTTCCCCCATAGCACATATTCAGCATGACCCTTCGTCCGAAGAGCATATATACCATGGCCCCTTGCCCAAAGCAGAACTTCACAACATTAGGACAAAAGCTCAGAAAAGCAGGGAAAAACCTGGCCATGGCCCTACGCCCCAAGCAGAACTTCAGGAACATAGGACAGAACCTGATGTTTGGATAGATTGGAGTGAAATCCGACATTTGTGGTTAGACCGTGATGTTTGGATAGATTGGAGTAAAATCCGACACTTGTGGTTAGGCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

540

Amino Acids

60.27

Weight (kDa)

5.81

Isoelectric Point (pI)

54.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 223, 271, 983
AclWI GGATC 2 cut(s) 424, 1016
AcoI YGGCCR 1 cut(s) 1471
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 4 cut(s) 837, 1247, 1338, 1484
AfaI GTAC 2 cut(s) 136, 1323
AfiI CCNNNNNNNGG 2 cut(s) 874, 1475
AflIII ACRYGT 1 cut(s) 1050
AgsI TTSAA 5 cut(s) 209, 341, 461, 862, 938
AhdI GACNNNNNGTC 1 cut(s) 1381
AjnI CCWGG 5 cut(s) 25, 325, 534, 873, 1468
AjuI GAANNNNNNNTTGG 4 cut(s) 1281, 1313, 1482, 1514
AluBI AGCT 6 cut(s) 76, 318, 664, 719, 857, 1448
AluI AGCT 6 cut(s) 76, 318, 664, 719, 857, 1448
Alw21I GWGCWC 1 cut(s) 946
Alw26I GTCTC 1 cut(s) 821
AlwI GGATC 2 cut(s) 424, 1016
AlwNI CAGNNNCTG 1 cut(s) 1520
AoxI GGCC 8 cut(s) 784, 1204, 1270, 1306, 1336, 1405, 1471, 1477
ApeKI GCWGC 2 cut(s) 125, 628
ApoI RAATTY 1 cut(s) 246
ArsI GACNNNNNNTTYG 2 cut(s) 258, 290
AspLEI GCGC 1 cut(s) 1294
AspS9I GGNCC 8 cut(s) 407, 699, 1205, 1270, 1307, 1337, 1406, 1478
AsuHPI GGTGA 2 cut(s) 921, 929
AvaII GGWCC 2 cut(s) 407, 699
BalI TGGCCA 1 cut(s) 1473
BanII GRGCYC 1 cut(s) 490
BauI CACGAG 2 cut(s) 720, 945
BbsI GAAGAC 1 cut(s) 51
Bbv12I GWGCWC 1 cut(s) 946
BbvI GCAGC 2 cut(s) 112, 640
BccI CCATC 3 cut(s) 512, 722, 1169
BciT130I CCWGG 5 cut(s) 27, 327, 536, 875, 1470
BcoDI GTCTC 1 cut(s) 821
BfaI CTAG 1 cut(s) 899
BfmI CTRYAG 2 cut(s) 126, 626
BisI GCNGC 2 cut(s) 126, 629
BlsI GCNGC 2 cut(s) 127, 630
Bme1390I CCNGG 5 cut(s) 27, 327, 536, 875, 1470
Bme18I GGWCC 2 cut(s) 407, 699
BmeRI GACNNNNNGTC 1 cut(s) 1381
BmgT120I GGNCC 8 cut(s) 407, 699, 1205, 1270, 1307, 1337, 1406, 1478
BmiI GGNNCC 3 cut(s) 523, 1107, 1408
BmrFI CCNGG 5 cut(s) 27, 327, 536, 875, 1470
BmrI ACTGGG 1 cut(s) 983
BmsI GCATC 2 cut(s) 112, 811
BmuI ACTGGG 1 cut(s) 983
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 2 cut(s) 307, 339
BpmI CTGGAG 2 cut(s) 77, 518
BpuEI CTTGAG 2 cut(s) 978, 1088
BsaBI GATNNNNATC 1 cut(s) 732
BsaJI CCNNGG 4 cut(s) 1150, 1273, 1402, 1474
BsaWI WCCGGW 1 cut(s) 1024
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 217, 247
Bsc4I CCNNNNNNNGG 2 cut(s) 874, 1475
Bse1I ACTGG 2 cut(s) 741, 978
Bse3DI GCAATG 1 cut(s) 1237
Bse8I GATNNNNATC 1 cut(s) 732
BseBI CCWGG 5 cut(s) 27, 327, 536, 875, 1470
BseDI CCNNGG 4 cut(s) 1150, 1273, 1402, 1474
BseGI GGATG 3 cut(s) 127, 642, 1005
BseJI GATNNNNATC 1 cut(s) 732
BseLI CCNNNNNNNGG 2 cut(s) 874, 1475
BseMI GCAATG 1 cut(s) 1237
BseMII CTCAG 6 cut(s) 86, 106, 245, 1023, 1068, 1463
BseNI ACTGG 2 cut(s) 741, 978
BseRI GAGGAG 2 cut(s) 12, 1332
BseXI GCAGC 2 cut(s) 112, 640
BshFI GGCC 8 cut(s) 786, 1206, 1272, 1308, 1338, 1407, 1473, 1479
BsiHKAI GWGCWC 1 cut(s) 946
BsiSI CCGG 1 cut(s) 1025
BslFI GGGAC 2 cut(s) 380, 1204
BslI CCNNNNNNNGG 2 cut(s) 874, 1475
BsmAI GTCTC 1 cut(s) 821
BsmFI GGGAC 2 cut(s) 380, 1204
BsnI GGCC 8 cut(s) 786, 1206, 1272, 1308, 1338, 1407, 1473, 1479
Bsp1286I GDGCHC 2 cut(s) 490, 946
Bsp143I GATC 4 cut(s) 416, 747, 919, 1021
Bsp19I CCATGG 3 cut(s) 1273, 1402, 1474
BspACI CCGC 3 cut(s) 223, 271, 983
BspANI GGCC 8 cut(s) 786, 1206, 1272, 1308, 1338, 1407, 1473, 1479
BspCNI CTCAG 6 cut(s) 85, 105, 246, 1024, 1069, 1462
BspLI GGNNCC 3 cut(s) 523, 1107, 1408
BspMAI CTGCAG 2 cut(s) 130, 630
BspPI GGATC 2 cut(s) 424, 1016
BspQI GCTCTTC 1 cut(s) 1383
BsrDI GCAATG 1 cut(s) 1237
BsrI ACTGG 2 cut(s) 741, 978
BssECI CCNNGG 4 cut(s) 1150, 1273, 1402, 1474
BssMI GATC 4 cut(s) 416, 747, 919, 1021
BssSI CACGAG 2 cut(s) 720, 945
BssT1I CCWWGG 4 cut(s) 1150, 1273, 1402, 1474
Bst2BI CACGAG 2 cut(s) 720, 945
Bst2UI CCWGG 5 cut(s) 27, 327, 536, 875, 1470
Bst4CI ACNGT 4 cut(s) 170, 394, 1122, 1568
Bst6I CTCTTC 1 cut(s) 1383
BstC8I GCNNGC 2 cut(s) 987, 1074
BstDEI CTNAG 6 cut(s) 72, 92, 254, 1032, 1077, 1449
BstDSI CCRYGG 3 cut(s) 1273, 1402, 1474
BstF5I GGATG 3 cut(s) 127, 642, 1005
BstHHI GCGC 1 cut(s) 1294
BstKTI GATC 4 cut(s) 419, 750, 922, 1024
BstMAI GTCTC 1 cut(s) 821
BstMBI GATC 4 cut(s) 416, 747, 919, 1021
BstMWI GCNNNNNNNGC 1 cut(s) 1300
BstNI CCWGG 5 cut(s) 27, 327, 536, 875, 1470
BstNSI RCATGY 2 cut(s) 1054, 1298
BstSCI CCNGG 5 cut(s) 25, 325, 534, 873, 1468
BstSFI CTRYAG 2 cut(s) 126, 626
BstV1I GCAGC 2 cut(s) 112, 640
BstV2I GAAGAC 1 cut(s) 51
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BsuRI GGCC 8 cut(s) 786, 1206, 1272, 1308, 1338, 1407, 1473, 1479
BtgI CCRYGG 3 cut(s) 1273, 1402, 1474
BtsCI GGATG 3 cut(s) 127, 642, 1005
BtsI GCAGTG 2 cut(s) 37, 1024
BtsIMutI CAGTG 5 cut(s) 37, 175, 748, 1024, 1118
Cac8I GCNNGC 2 cut(s) 987, 1074
CaiI CAGNNNCTG 1 cut(s) 1520
CfoI GCGC 1 cut(s) 1294
Cfr13I GGNCC 8 cut(s) 407, 699, 1205, 1270, 1307, 1337, 1406, 1478
CseI GACGC 1 cut(s) 465
Csp6I GTAC 2 cut(s) 135, 1322
CviQI GTAC 2 cut(s) 135, 1322
DdeI CTNAG 6 cut(s) 72, 92, 254, 1032, 1077, 1449
DpnI GATC 4 cut(s) 418, 749, 921, 1023
DpnII GATC 4 cut(s) 416, 747, 919, 1021
DraI TTTAAA 1 cut(s) 883
DriI GACNNNNNGTC 1 cut(s) 1381
EaeI YGGCCR 1 cut(s) 1471
Eam1104I CTCTTC 1 cut(s) 1383
Eam1105I GACNNNNNGTC 1 cut(s) 1381
EarI CTCTTC 1 cut(s) 1383
EciI GGCGGA 1 cut(s) 972
Eco130I CCWWGG 4 cut(s) 1150, 1273, 1402, 1474
Eco24I GRGCYC 1 cut(s) 490
Eco47I GGWCC 2 cut(s) 407, 699
Eco57I CTGAAG 4 cut(s) 837, 1247, 1338, 1484
EcoO109I RGGNCCY 1 cut(s) 1205
EcoRII CCWGG 5 cut(s) 25, 325, 534, 873, 1468
EcoT14I CCWWGG 4 cut(s) 1150, 1273, 1402, 1474
EcoT38I GRGCYC 1 cut(s) 490
ErhI CCWWGG 4 cut(s) 1150, 1273, 1402, 1474
FalI AAGNNNNNCTT 6 cut(s) 648, 680, 1410, 1442, 1482, 1514
FaqI GGGAC 2 cut(s) 380, 1204
Fnu4HI GCNGC 2 cut(s) 126, 629
FokI GGATG 3 cut(s) 134, 649, 992
FriOI GRGCYC 1 cut(s) 490
Fsp4HI GCNGC 2 cut(s) 126, 629
FspBI CTAG 1 cut(s) 899
GlaI GCGC 1 cut(s) 1293
GluI GCNGC 2 cut(s) 126, 629
GsuI CTGGAG 2 cut(s) 77, 518
HaeIII GGCC 8 cut(s) 786, 1206, 1272, 1308, 1338, 1407, 1473, 1479
HapII CCGG 1 cut(s) 1025
HgaI GACGC 1 cut(s) 465
HhaI GCGC 1 cut(s) 1294
Hin6I GCGC 1 cut(s) 1292
HinP1I GCGC 1 cut(s) 1292
HincII GTYRAC 1 cut(s) 1048
HindII GTYRAC 1 cut(s) 1048
HinfI GANTC 7 cut(s) 65, 176, 274, 602, 677, 829, 1258
HpaII CCGG 1 cut(s) 1025
HphI GGTGA 2 cut(s) 921, 929
Hpy166II GTNNAC 2 cut(s) 137, 1048
Hpy188I TCNGA 8 cut(s) 95, 481, 682, 798, 1387, 1452, 1550, 1598
Hpy188III TCNNGA 5 cut(s) 180, 420, 722, 751, 1502
Hpy8I GTNNAC 2 cut(s) 137, 1048
HpyAV CCTTC 6 cut(s) 125, 748, 1224, 1254, 1359, 1389
HpyCH4III ACNGT 4 cut(s) 170, 394, 1122, 1568
HpyCH4IV ACGT 1 cut(s) 940
HpyCH4V TGCA 6 cut(s) 128, 332, 628, 714, 824, 989
HpyF10VI GCNNNNNNNGC 1 cut(s) 1300
HpyF3I CTNAG 6 cut(s) 72, 92, 254, 1032, 1077, 1449
HpySE526I ACGT 1 cut(s) 940
HspAI GCGC 1 cut(s) 1292
Kzo9I GATC 4 cut(s) 416, 747, 919, 1021
LguI GCTCTTC 1 cut(s) 1383
LmnI GCTCC 3 cut(s) 58, 661, 1140
Lsp1109I GCAGC 2 cut(s) 112, 640
LweI GCATC 2 cut(s) 112, 811
MaeI CTAG 1 cut(s) 899
MaeII ACGT 1 cut(s) 940
MaeIII GTNAC 4 cut(s) 907, 1027, 1053, 1116
MalI GATC 4 cut(s) 418, 749, 921, 1023
MboI GATC 4 cut(s) 416, 747, 919, 1021
MflI RGATCY 1 cut(s) 416
MhlI GDGCHC 2 cut(s) 490, 946
MlsI TGGCCA 1 cut(s) 1473
MluCI AATT 6 cut(s) 246, 359, 502, 760, 1155, 1180
MluNI TGGCCA 1 cut(s) 1473
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 2 cut(s) 493, 1573
Mox20I TGGCCA 1 cut(s) 1473
MscI TGGCCA 1 cut(s) 1473
MseI TTAA 4 cut(s) 306, 759, 780, 882
MslI CAYNNNNRTG 2 cut(s) 707, 842
Msp20I TGGCCA 1 cut(s) 1473
MspI CCGG 1 cut(s) 1025
MspR9I CCNGG 5 cut(s) 27, 327, 536, 875, 1470
MvaI CCWGG 5 cut(s) 27, 327, 536, 875, 1470
MwoI GCNNNNNNNGC 1 cut(s) 1300
NcoI CCATGG 3 cut(s) 1273, 1402, 1474
NdeII GATC 4 cut(s) 416, 747, 919, 1021
NlaIV GGNNCC 3 cut(s) 523, 1107, 1408
NmuCI GTSAC 2 cut(s) 907, 1116
NspI RCATGY 2 cut(s) 1054, 1298
PciI ACATGT 1 cut(s) 1050
PciSI GCTCTTC 1 cut(s) 1383
PfeI GAWTC 6 cut(s) 65, 176, 274, 602, 677, 1258
PfoI TCCNGGA 1 cut(s) 534
PkrI GCNGC 2 cut(s) 127, 630
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
PscI ACATGT 1 cut(s) 1050
Psp6I CCWGG 5 cut(s) 25, 325, 534, 873, 1468
PspGI CCWGG 5 cut(s) 25, 325, 534, 873, 1468
PspN4I GGNNCC 3 cut(s) 523, 1107, 1408
PspPI GGNCC 8 cut(s) 407, 699, 1205, 1270, 1307, 1337, 1406, 1478
PstI CTGCAG 2 cut(s) 130, 630
PstNI CAGNNNCTG 1 cut(s) 1520
PsuI RGATCY 1 cut(s) 416
RsaI GTAC 2 cut(s) 136, 1323
RsaNI GTAC 2 cut(s) 135, 1322
RseI CAYNNNNRTG 2 cut(s) 707, 842
SapI GCTCTTC 1 cut(s) 1383
SaqAI TTAA 4 cut(s) 306, 759, 780, 882
SatI GCNGC 2 cut(s) 126, 629
Sau3AI GATC 4 cut(s) 416, 747, 919, 1021
Sau96I GGNCC 8 cut(s) 407, 699, 1205, 1270, 1307, 1337, 1406, 1478
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 5 cut(s) 27, 327, 536, 875, 1470
SduI GDGCHC 2 cut(s) 490, 946
SfaNI GCATC 2 cut(s) 112, 811
SfcI CTRYAG 2 cut(s) 126, 626
SinI GGWCC 2 cut(s) 407, 699
SmiMI CAYNNNNRTG 2 cut(s) 707, 842
SmlI CTYRAG 2 cut(s) 993, 1067
SmoI CTYRAG 2 cut(s) 993, 1067
Sse9I AATT 6 cut(s) 246, 359, 502, 760, 1155, 1180
SsiI CCGC 3 cut(s) 223, 271, 983
SspMI CTAG 1 cut(s) 899
StyD4I CCNGG 5 cut(s) 25, 325, 534, 873, 1468
StyI CCWWGG 4 cut(s) 1150, 1273, 1402, 1474
TaaI ACNGT 4 cut(s) 170, 394, 1122, 1568
TaiI ACGT 1 cut(s) 943
TaqI TCGA 2 cut(s) 181, 832
TasI AATT 6 cut(s) 246, 359, 502, 760, 1155, 1180
TatI WGTACW 1 cut(s) 1321
TfiI GAWTC 6 cut(s) 65, 176, 274, 602, 677, 1258
Tru1I TTAA 4 cut(s) 306, 759, 780, 882
Tru9I TTAA 4 cut(s) 306, 759, 780, 882
TscAI CASTG 5 cut(s) 37, 175, 748, 1024, 1125
TseFI GTSAC 2 cut(s) 907, 1116
TseI GCWGC 2 cut(s) 125, 628
Tsp45I GTSAC 2 cut(s) 907, 1116
TspDTI ATGAA 7 cut(s) 125, 495, 624, 653, 831, 1193, 1271
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 5 cut(s) 37, 175, 748, 1024, 1125
VpaK11BI GGWCC 2 cut(s) 407, 699
XapI RAATTY 1 cut(s) 246
XceI RCATGY 2 cut(s) 1054, 1298
XspI CTAG 1 cut(s) 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.