pycom01g05980

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014642
Physical Location & Seq
Reverse (-)
5998737 .. 6000590
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1047 bp
ATGGTCCGCTGCTTTCTTACATATGTAGTCCTCCTCCTATGCCCATTAAAAACAAGTCTACTGCTCTTAGAAAGAGAAGAGAGAGAGAGAGAGAGAGAGAGAAGCCAAGAGAAAGCTCTAATGGTTGTAGTACCAACTCTTTCAGTATTCATCCTCCTCAACCTTGCCACAGCCGCCGCCGCAGACTCTCCTGCATGCAACCCCACATGTGGCTCCCTGCAAGTCAAATACCCTTTTGGCACAGGCCCTGGTTGTGGCTCCTCAATTTTCCAATCCTACATAACCTGTACATTTACCAATAATCAGCAGCTCCTCCTCCTCACCACCCACACTGGCTTATACCCTATCACTTCAATTTCCTACTCCACCCAAACCATAACCCTAACCCCACCCTCCATGTTCAACTGCACCTCCATGCAACCCTCCCTCTCCAACTTCGGCCTTGACTGGGCCTCCCCATTTCAGCTCGGCCCATCAACCTTCATCCTCTTGTCCTGCCAGCCCCCCACCTCTTCCCTCACCCTCAAACCCTCCGGCATGCCCGTCTGTGATCCCTGCTACTCTCACCTCTGTGCTTCTATCTACACATGTCCATCCGTGGTTGGTCTTGGCCTCCCCTTGTTCCCTCCCACAAACACGTGCTGCGTGTATTCACCTGGGAATCTTGACGCTAAGGGTGAGTTGGACCTCCACGGGTTAAGGTGCACGTTTACATCCGTTGTATCACTCGGAGACTACCTGACAGACCCAGTGCAGTGGGAGTATGGGGTGGCGTTGAAGTACAGTCACGGTGGTTTGGACAGTGGCATTGTCGATGCAAAGTGCAAGAGCTGTGAGATGAGCGATGGGGTTTGTGGGTACAGGGTTGATGATCATGATCAGTTCCTTTGTGTGTGTAAGAATGGCTACAACACCTCCTCCGATTGCCTAAATAATTACACTCCAGATTCGAAGCCCCTTTGGGGCTCCGGCGCGTCTGATGATTTACCAGTTGTTAATTGGAAAATATGGTCTGCGCTCGTGGCTGGCCTGATGATAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

349

Amino Acids

37.67

Weight (kDa)

5.06

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 66 - 129 4.4e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 58
AccII CGCG 1 cut(s) 974
AciI CCGC 4 cut(s) 7, 174, 177, 180
AclWI GGATC 1 cut(s) 545
AcvI CACGTG 1 cut(s) 639
AfaI GTAC 4 cut(s) 132, 289, 782, 860
AfiI CCNNNNNNNGG 4 cut(s) 209, 254, 448, 962
AflIII ACRYGT 3 cut(s) 206, 587, 636
AgsI TTSAA 3 cut(s) 354, 403, 778
AjnI CCWGG 2 cut(s) 247, 655
AleI CACNNNNGTG 1 cut(s) 570
AluBI AGCT 4 cut(s) 116, 310, 466, 831
AluI AGCT 4 cut(s) 116, 310, 466, 831
Alw21I GWGCWC 1 cut(s) 707
Alw26I GTCTC 1 cut(s) 726
Alw44I GTGCAC 1 cut(s) 703
AlwI GGATC 1 cut(s) 545
AlwNI CAGNNNCTG 1 cut(s) 248
AoxI GGCC 6 cut(s) 244, 439, 450, 469, 610, 1027
ApaLI GTGCAC 1 cut(s) 703
ApeKI GCWGC 3 cut(s) 9, 307, 642
AspLEI GCGC 2 cut(s) 974, 1018
AspS9I GGNCC 5 cut(s) 4, 245, 450, 470, 685
AsuHPI GGTGA 5 cut(s) 313, 511, 557, 645, 689
AsuII TTCGAA 1 cut(s) 950
AvaII GGWCC 2 cut(s) 4, 685
BaeGI GKGCMC 1 cut(s) 707
BanII GRGCYC 1 cut(s) 968
BauI CACGAG 1 cut(s) 1019
BbrPI CACGTG 1 cut(s) 639
Bbv12I GWGCWC 1 cut(s) 707
BbvI GCAGC 2 cut(s) 319, 629
BccI CCATC 3 cut(s) 481, 601, 839
BciT130I CCWGG 2 cut(s) 249, 657
BclI TGATCA 2 cut(s) 871, 877
BcoDI GTCTC 1 cut(s) 726
BisI GCNGC 6 cut(s) 10, 174, 177, 180, 308, 643
BlsI GCNGC 6 cut(s) 11, 175, 178, 181, 309, 644
Bme1390I CCNGG 2 cut(s) 249, 657
Bme18I GGWCC 2 cut(s) 4, 685
BmgT120I GGNCC 5 cut(s) 4, 245, 450, 470, 685
BmiI GGNNCC 3 cut(s) 214, 259, 967
BmrFI CCNGG 2 cut(s) 249, 657
BmrI ACTGGG 2 cut(s) 457, 743
BmsI GCATC 1 cut(s) 805
BmuI ACTGGG 2 cut(s) 457, 743
BpmI CTGGAG 1 cut(s) 927
Bpu10I CCTNAGC 1 cut(s) 672
Bpu14I TTCGAA 1 cut(s) 950
BsaAI YACGTR 1 cut(s) 639
BsaBI GATNNNNATC 1 cut(s) 876
BsaJI CCNNGG 4 cut(s) 247, 597, 656, 691
BsaXI ACNNNNNCTCC 8 cut(s) 395, 425, 437, 467, 899, 902, 929, 932
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 254, 448, 962
Bse1I ACTGG 4 cut(s) 337, 452, 749, 989
Bse8I GATNNNNATC 1 cut(s) 876
BseBI CCWGG 2 cut(s) 249, 657
BseDI CCNNGG 4 cut(s) 247, 597, 656, 691
BseGI GGATG 4 cut(s) 150, 483, 593, 713
BseJI GATNNNNATC 1 cut(s) 876
BseLI CCNNNNNNNGG 4 cut(s) 209, 254, 448, 962
BseNI ACTGG 4 cut(s) 337, 452, 749, 989
BseRI GAGGAG 7 cut(s) 23, 146, 250, 302, 305, 308, 907
BseSI GKGCMC 1 cut(s) 707
BseXI GCAGC 2 cut(s) 319, 629
BsgI GTGCAG 2 cut(s) 391, 773
Bsh1236I CGCG 1 cut(s) 974
BshFI GGCC 6 cut(s) 246, 441, 452, 471, 612, 1029
BsiHKAI GWGCWC 1 cut(s) 707
BsiSI CCGG 2 cut(s) 534, 969
BslI CCNNNNNNNGG 4 cut(s) 209, 254, 448, 962
BsmAI GTCTC 1 cut(s) 726
BsnI GGCC 6 cut(s) 246, 441, 452, 471, 612, 1029
Bsp119I TTCGAA 1 cut(s) 950
Bsp1286I GDGCHC 2 cut(s) 707, 968
Bsp1407I TGTACA 1 cut(s) 287
Bsp143I GATC 3 cut(s) 550, 871, 877
BspACI CCGC 4 cut(s) 7, 174, 177, 180
BspANI GGCC 6 cut(s) 246, 441, 452, 471, 612, 1029
BspFNI CGCG 1 cut(s) 974
BspHI TCATGA 1 cut(s) 874
BspLI GGNNCC 3 cut(s) 214, 259, 967
BspPI GGATC 1 cut(s) 545
BspT104I TTCGAA 1 cut(s) 950
BsrGI TGTACA 1 cut(s) 287
BsrI ACTGG 4 cut(s) 337, 452, 749, 989
BssECI CCNNGG 4 cut(s) 247, 597, 656, 691
BssMI GATC 3 cut(s) 550, 871, 877
BssSI CACGAG 1 cut(s) 1019
Bst2BI CACGAG 1 cut(s) 1019
Bst2UI CCWGG 2 cut(s) 249, 657
Bst4CI ACNGT 3 cut(s) 785, 791, 803
Bst6I CTCTTC 2 cut(s) 72, 517
BstAUI TGTACA 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 639
BstBI TTCGAA 1 cut(s) 950
BstC8I GCNNGC 4 cut(s) 196, 500, 539, 1027
BstDEI CTNAG 2 cut(s) 67, 672
BstDSI CCRYGG 2 cut(s) 597, 691
BstF5I GGATG 4 cut(s) 150, 483, 593, 713
BstFNI CGCG 1 cut(s) 974
BstHHI GCGC 2 cut(s) 974, 1018
BstKTI GATC 3 cut(s) 553, 874, 880
BstMAI GTCTC 1 cut(s) 726
BstMBI GATC 3 cut(s) 550, 871, 877
BstMWI GCNNNNNNNGC 3 cut(s) 173, 179, 1022
BstNI CCWGG 2 cut(s) 249, 657
BstNSI RCATGY 4 cut(s) 198, 210, 541, 591
BstSCI CCNGG 2 cut(s) 247, 655
BstSLI GKGCMC 1 cut(s) 707
BstUI CGCG 1 cut(s) 974
BstV1I GCAGC 2 cut(s) 319, 629
BstXI CCANNNNNNTGG 1 cut(s) 756
BsuRI GGCC 6 cut(s) 246, 441, 452, 471, 612, 1029
BtgI CCRYGG 2 cut(s) 597, 691
BtgZI GCGATG 1 cut(s) 858
BtsCI GGATG 4 cut(s) 150, 483, 593, 713
BtsI GCAGTG 1 cut(s) 761
BtsIMutI CAGTG 4 cut(s) 330, 756, 761, 808
Cac8I GCNNGC 4 cut(s) 196, 500, 539, 1027
CaiI CAGNNNCTG 1 cut(s) 248
CciI TCATGA 1 cut(s) 874
CfoI GCGC 2 cut(s) 974, 1018
Cfr13I GGNCC 5 cut(s) 4, 245, 450, 470, 685
CseI GACGC 2 cut(s) 677, 963
Csp6I GTAC 4 cut(s) 131, 288, 781, 859
CviAII CATG 7 cut(s) 195, 207, 397, 415, 538, 588, 875
CviQI GTAC 4 cut(s) 131, 288, 781, 859
DdeI CTNAG 2 cut(s) 67, 672
DpnI GATC 3 cut(s) 552, 873, 879
DpnII GATC 3 cut(s) 550, 871, 877
Eam1104I CTCTTC 2 cut(s) 72, 517
EarI CTCTTC 2 cut(s) 72, 517
Eco24I GRGCYC 1 cut(s) 968
Eco47I GGWCC 2 cut(s) 4, 685
Eco72I CACGTG 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 247, 655
EcoT38I GRGCYC 1 cut(s) 968
FaeI CATG 7 cut(s) 198, 210, 400, 418, 541, 591, 878
FatI CATG 7 cut(s) 194, 206, 396, 414, 537, 587, 874
FauNDI CATATG 1 cut(s) 22
FbaI TGATCA 2 cut(s) 871, 877
FblI GTMKAC 1 cut(s) 58
Fnu4HI GCNGC 6 cut(s) 10, 174, 177, 180, 308, 643
FokI GGATG 4 cut(s) 137, 470, 580, 700
FriOI GRGCYC 1 cut(s) 968
Fsp4HI GCNGC 6 cut(s) 10, 174, 177, 180, 308, 643
GlaI GCGC 2 cut(s) 973, 1017
GluI GCNGC 6 cut(s) 10, 174, 177, 180, 308, 643
GsuI CTGGAG 1 cut(s) 927
HaeIII GGCC 6 cut(s) 246, 441, 452, 471, 612, 1029
HapII CCGG 2 cut(s) 534, 969
HgaI GACGC 2 cut(s) 677, 963
HhaI GCGC 2 cut(s) 974, 1018
Hin1II CATG 7 cut(s) 198, 210, 400, 418, 541, 591, 878
Hin6I GCGC 2 cut(s) 972, 1016
HinP1I GCGC 2 cut(s) 972, 1016
HinfI GANTC 3 cut(s) 185, 661, 947
HpaII CCGG 2 cut(s) 534, 969
HphI GGTGA 5 cut(s) 313, 511, 557, 645, 689
Hpy166II GTNNAC 3 cut(s) 59, 705, 711
Hpy188I TCNGA 3 cut(s) 731, 922, 979
Hpy188III TCNNGA 3 cut(s) 665, 875, 944
Hpy8I GTNNAC 3 cut(s) 59, 705, 711
HpyAV CCTTC 1 cut(s) 490
HpyCH4III ACNGT 3 cut(s) 785, 791, 803
HpyCH4IV ACGT 2 cut(s) 638, 707
HpyCH4V TGCA 9 cut(s) 194, 198, 220, 408, 418, 705, 754, 818, 825
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 179, 1022
HpyF3I CTNAG 2 cut(s) 67, 672
HpySE526I ACGT 2 cut(s) 638, 707
Hsp92II CATG 7 cut(s) 198, 210, 400, 418, 541, 591, 878
HspAI GCGC 2 cut(s) 972, 1016
Ksp22I TGATCA 2 cut(s) 871, 877
Kzo9I GATC 3 cut(s) 550, 871, 877
LmnI GCTCC 4 cut(s) 218, 263, 315, 971
Lsp1109I GCAGC 2 cut(s) 319, 629
LweI GCATC 1 cut(s) 805
MaeII ACGT 2 cut(s) 638, 707
MaeIII GTNAC 1 cut(s) 785
MalI GATC 3 cut(s) 552, 873, 879
MboI GATC 3 cut(s) 550, 871, 877
MboII GAAGA 2 cut(s) 89, 504
MhlI GDGCHC 2 cut(s) 707, 968
MluCI AATT 4 cut(s) 264, 354, 934, 997
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 456, 663
MseI TTAA 3 cut(s) 47, 698, 996
MslI CAYNNNNRTG 2 cut(s) 413, 570
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 2 cut(s) 534, 969
MspR9I CCNGG 2 cut(s) 249, 657
MvaI CCWGG 2 cut(s) 249, 657
MvnI CGCG 1 cut(s) 974
MwoI GCNNNNNNNGC 3 cut(s) 173, 179, 1022
NdeI CATATG 1 cut(s) 22
NdeII GATC 3 cut(s) 550, 871, 877
NlaIII CATG 7 cut(s) 198, 210, 400, 418, 541, 591, 878
NlaIV GGNNCC 3 cut(s) 214, 259, 967
NmeAIII GCCGAG 1 cut(s) 447
NmuCI GTSAC 1 cut(s) 785
NspI RCATGY 4 cut(s) 198, 210, 541, 591
NspV TTCGAA 1 cut(s) 950
OliI CACNNNNGTG 1 cut(s) 570
PaeI GCATGC 2 cut(s) 198, 541
PagI TCATGA 1 cut(s) 874
PciI ACATGT 2 cut(s) 206, 587
PfeI GAWTC 2 cut(s) 661, 947
PkrI GCNGC 6 cut(s) 11, 175, 178, 181, 309, 644
PleI GAGTC 1 cut(s) 179
PmaCI CACGTG 1 cut(s) 639
PmlI CACGTG 1 cut(s) 639
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 639
PscI ACATGT 2 cut(s) 206, 587
Psp6I CCWGG 2 cut(s) 247, 655
PspCI CACGTG 1 cut(s) 639
PspGI CCWGG 2 cut(s) 247, 655
PspN4I GGNNCC 3 cut(s) 214, 259, 967
PspPI GGNCC 5 cut(s) 4, 245, 450, 470, 685
PstNI CAGNNNCTG 1 cut(s) 248
RsaI GTAC 4 cut(s) 132, 289, 782, 860
RsaNI GTAC 4 cut(s) 131, 288, 781, 859
RseI CAYNNNNRTG 2 cut(s) 413, 570
SaqAI TTAA 3 cut(s) 47, 698, 996
SatI GCNGC 6 cut(s) 10, 174, 177, 180, 308, 643
Sau3AI GATC 3 cut(s) 550, 871, 877
Sau96I GGNCC 5 cut(s) 4, 245, 450, 470, 685
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 249, 657
SduI GDGCHC 2 cut(s) 707, 968
SfaNI GCATC 1 cut(s) 805
SfuI TTCGAA 1 cut(s) 950
SinI GGWCC 2 cut(s) 4, 685
SmiMI CAYNNNNRTG 2 cut(s) 413, 570
SphI GCATGC 2 cut(s) 198, 541
Sse9I AATT 4 cut(s) 264, 354, 934, 997
SsiI CCGC 4 cut(s) 7, 174, 177, 180
StyD4I CCNGG 2 cut(s) 247, 655
TaaI ACNGT 3 cut(s) 785, 791, 803
TaiI ACGT 2 cut(s) 641, 710
TaqI TCGA 2 cut(s) 813, 950
TasI AATT 4 cut(s) 264, 354, 934, 997
TatI WGTACW 2 cut(s) 287, 780
TauI GCSGC 3 cut(s) 176, 179, 182
TfiI GAWTC 2 cut(s) 661, 947
Tru1I TTAA 3 cut(s) 47, 698, 996
Tru9I TTAA 3 cut(s) 47, 698, 996
TscAI CASTG 4 cut(s) 337, 756, 761, 808
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 3 cut(s) 9, 307, 642
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 2 cut(s) 139, 472
TspGWI ACGGA 2 cut(s) 586, 706
TspRI CASTG 4 cut(s) 337, 756, 761, 808
VneI GTGCAC 1 cut(s) 703
VpaK11BI GGWCC 2 cut(s) 4, 685
XceI RCATGY 4 cut(s) 198, 210, 541, 591
XcmI CCANNNNNNNNNTGG 1 cut(s) 996
XmiI GTMKAC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.