pycom01g07540

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009758
Physical Location & Seq
Reverse (-)
8478709 .. 8480406
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 969 bp
ATGGAATATGTAACCGTTGCAAGTTGGGGAGCTTACCTTTTGTCCAGGGACATTCTAACAGTGTCATTTGCACCAAGAGACACACATGAAGCGCAAGTTCAATTTGCTCTTGAAAGAGGAGTTCCTGCATTGATTGGCATTCTTGCTTCCAAAAGACTTCCTTACCCCTCAAGAGCTTTTGACATGGCTCACTGCTCTCGATGCCTCATCCCCTGGGGCCAATATGGTAAATTCACCGATTTTGATCATGCAGATGGACTTTACTTGATCGAAGTTGATCGAGTTTTACGCCCCGGTGGGTACTGGATTCTTTCCGGGCCACCAATAAACTGGGAGAATCACTGGAAAGGATGGGAAAGAACAGCTGAAGATCTTAAAGCTGAGCAGACAACGATTGAGAATGTAGCAAAAAGCTTATGTTGGAAGAAACTGAAGCAAAAGGGTGACCTTGCAATTTGGCAAAAACCCACCAACCATGTTCACTGTAAGGTCACCAGAAAGCTCTTCAAGAAACCATCCTTCTGCCAAGCTCAAGATCCTGACACTGCATGGTATACGAAAATGGAGGATTGTTTAACCCCACTTCCCGGAGTGAACAACATAAAAGAAATTGCAGGAGGGCAATTGGCTAAATGGCCAGAGAGGCTAAACACAGTCCCTCCAAGAATCAGCAGTGGGAGGCTGACAGGAATCACAGCTGAGAGCTTCAGAGAAAACACAGAGCTATGGAAGAAAAGAGTAGAATATTACAAGACCGTGGATTATCAGTTGGCAGAGCCCGGGAGGTACCGGAATCTGCTCGATATGAACGCTTACTTGGGCGGCTTTGCAGCTGTACTTGTTCATGACCCTGTGTGGGTTATGAACATTGTCCCTGTTGAGGCTGAGGTTAACACCCTTGGAGCCATTTATGAACGAGGATTGATTGGAACTTATCAGAATTGGTATGCTCTTTCCCCCATGCAATAA

Protein Analysis

323

Amino Acids

36.78

Weight (kDa)

8.4

Isoelectric Point (pI)

29.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 5 - 315 2e-120 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 786
AccB1I GGYRCC 1 cut(s) 786
AccI GTMKAC 1 cut(s) 554
AciI CCGC 1 cut(s) 822
AclWI GGATC 1 cut(s) 530
AcoI YGGCCR 1 cut(s) 635
AcsI RAATTY 1 cut(s) 230
AcuI CTGAAG 3 cut(s) 387, 452, 691
AfaI GTAC 3 cut(s) 302, 788, 837
AfiI CCNNNNNNNGG 3 cut(s) 587, 856, 880
AgsI TTSAA 3 cut(s) 101, 113, 508
AjnI CCWGG 2 cut(s) 44, 212
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 1 cut(s) 530
Ama87I CYCGRG 1 cut(s) 779
AoxI GGCC 3 cut(s) 217, 317, 635
ApeKI GCWGC 1 cut(s) 830
ApoI RAATTY 1 cut(s) 230
Asp718I GGTACC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 2 cut(s) 217, 317
AsuC2I CCSGG 5 cut(s) 294, 316, 588, 780, 781
AsuHPI GGTGA 3 cut(s) 226, 455, 484
AvaI CYCGRG 1 cut(s) 779
BalI TGGCCA 1 cut(s) 637
BanI GGYRCC 1 cut(s) 786
BanII GRGCYC 1 cut(s) 780
BbvCI CCTCAGC 1 cut(s) 885
BbvI GCAGC 1 cut(s) 842
BccI CCATC 3 cut(s) 248, 345, 523
BciT130I CCWGG 2 cut(s) 46, 214
BclI TGATCA 1 cut(s) 244
BcnI CCSGG 5 cut(s) 294, 316, 588, 780, 781
BcoDI GTCTC 1 cut(s) 72
BglI GCCNNNNNGGC 1 cut(s) 643
BglII AGATCT 1 cut(s) 370
BisI GCNGC 2 cut(s) 823, 831
BlpI GCTNAGC 1 cut(s) 381
BlsI GCNGC 2 cut(s) 824, 832
Bme1390I CCNGG 7 cut(s) 46, 214, 294, 316, 588, 780, 781
BmeT110I CYCGRG 1 cut(s) 779
BmgT120I GGNCC 2 cut(s) 217, 317
BmiI GGNNCC 3 cut(s) 218, 788, 904
BmrFI CCNGG 7 cut(s) 46, 214, 294, 316, 588, 780, 781
BmrI ACTGGG 1 cut(s) 340
BmsI GCATC 1 cut(s) 191
BmuI ACTGGG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 885
Bpu1102I GCTNAGC 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 154, 516
BpuMI CCSGG 5 cut(s) 294, 316, 588, 780, 781
BsaBI GATNNNNATC 1 cut(s) 243
BsaJI CCNNGG 7 cut(s) 45, 212, 213, 292, 756, 779, 898
BsaWI WCCGGW 1 cut(s) 789
BsaXI ACNNNNNCTCC 2 cut(s) 643, 673
Bsc4I CCNNNNNNNGG 3 cut(s) 587, 856, 880
Bse1I ACTGG 3 cut(s) 308, 335, 347
Bse8I GATNNNNATC 1 cut(s) 243
BseBI CCWGG 2 cut(s) 46, 214
BseDI CCNNGG 7 cut(s) 45, 212, 213, 292, 756, 779, 898
BseGI GGATG 3 cut(s) 207, 356, 515
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 587, 856, 880
BseMII CTCAG 3 cut(s) 372, 690, 876
BseNI ACTGG 3 cut(s) 308, 335, 347
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 842
BshFI GGCC 3 cut(s) 219, 319, 637
BshNI GGYRCC 1 cut(s) 786
BsiHKCI CYCGRG 1 cut(s) 779
BsiSI CCGG 5 cut(s) 294, 315, 588, 780, 790
BslFI GGGAC 3 cut(s) 62, 641, 857
BslI CCNNNNNNNGG 3 cut(s) 587, 856, 880
BsmAI GTCTC 1 cut(s) 72
BsmFI GGGAC 3 cut(s) 62, 641, 857
BsmI GAATGC 1 cut(s) 138
BsnI GGCC 3 cut(s) 219, 319, 637
BsoBI CYCGRG 1 cut(s) 779
Bsp1286I GDGCHC 1 cut(s) 780
Bsp143I GATC 5 cut(s) 244, 267, 277, 370, 535
Bsp1720I GCTNAGC 1 cut(s) 381
BspACI CCGC 1 cut(s) 822
BspANI GGCC 3 cut(s) 219, 319, 637
BspCNI CTCAG 3 cut(s) 373, 691, 877
BspHI TCATGA 1 cut(s) 844
BspLI GGNNCC 3 cut(s) 218, 788, 904
BspPI GGATC 1 cut(s) 530
BspQI GCTCTTC 1 cut(s) 509
BspT107I GGYRCC 1 cut(s) 786
BsrI ACTGG 3 cut(s) 308, 335, 347
BssECI CCNNGG 7 cut(s) 45, 212, 213, 292, 756, 779, 898
BssMI GATC 5 cut(s) 244, 267, 277, 370, 535
BssNAI GTATAC 1 cut(s) 555
BssT1I CCWWGG 1 cut(s) 898
Bst1107I GTATAC 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 46, 214
Bst4CI ACNGT 5 cut(s) 16, 61, 485, 655, 757
Bst6I CTCTTC 1 cut(s) 509
BstDEI CTNAG 3 cut(s) 381, 699, 885
BstDSI CCRYGG 1 cut(s) 756
BstEII GGTNACC 2 cut(s) 443, 490
BstF5I GGATG 3 cut(s) 207, 356, 515
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 5 cut(s) 247, 270, 280, 373, 538
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 5 cut(s) 244, 267, 277, 370, 535
BstMWI GCNNNNNNNGC 2 cut(s) 201, 643
BstNI CCWGG 2 cut(s) 46, 214
BstPI GGTNACC 2 cut(s) 443, 490
BstSCI CCNGG 7 cut(s) 44, 212, 292, 314, 586, 778, 779
BstV1I GCAGC 1 cut(s) 842
BstX2I RGATCY 2 cut(s) 370, 535
BstXI CCANNNNNNTGG 1 cut(s) 330
BstYI RGATCY 2 cut(s) 370, 535
BstZ17I GTATAC 1 cut(s) 555
BsuRI GGCC 3 cut(s) 219, 319, 637
BtgI CCRYGG 1 cut(s) 756
BtsCI GGATG 3 cut(s) 207, 356, 515
BtsI GCAGTG 3 cut(s) 190, 543, 679
BtsIMutI CAGTG 6 cut(s) 66, 190, 340, 481, 543, 679
CciI TCATGA 1 cut(s) 844
CfoI GCGC 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 217, 317
Cfr9I CCCGGG 1 cut(s) 779
Csp6I GTAC 3 cut(s) 301, 787, 836
CviAII CATG 7 cut(s) 86, 184, 248, 476, 549, 845, 961
CviQI GTAC 3 cut(s) 301, 787, 836
DdeI CTNAG 3 cut(s) 381, 699, 885
DpnI GATC 5 cut(s) 246, 269, 279, 372, 537
DpnII GATC 5 cut(s) 244, 267, 277, 370, 535
EaeI YGGCCR 1 cut(s) 635
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 780
Eco57I CTGAAG 3 cut(s) 387, 452, 691
Eco88I CYCGRG 1 cut(s) 779
Eco91I GGTNACC 2 cut(s) 443, 490
EcoO65I GGTNACC 2 cut(s) 443, 490
EcoRII CCWGG 2 cut(s) 44, 212
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 7 cut(s) 89, 187, 251, 479, 552, 848, 964
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FaqI GGGAC 3 cut(s) 62, 641, 857
FatI CATG 7 cut(s) 85, 183, 247, 475, 548, 844, 960
FbaI TGATCA 1 cut(s) 244
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 2 cut(s) 823, 831
FokI GGATG 3 cut(s) 194, 363, 502
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 2 cut(s) 823, 831
GlaI GCGC 1 cut(s) 93
GluI GCNGC 2 cut(s) 823, 831
HaeIII GGCC 3 cut(s) 219, 319, 637
HapII CCGG 5 cut(s) 294, 315, 588, 780, 790
HhaI GCGC 1 cut(s) 94
Hin1II CATG 7 cut(s) 89, 187, 251, 479, 552, 848, 964
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 412
HinfI GANTC 5 cut(s) 307, 337, 666, 690, 793
HpaI GTTAAC 1 cut(s) 892
HpaII CCGG 5 cut(s) 294, 315, 588, 780, 790
HphI GGTGA 3 cut(s) 226, 455, 484
Hpy166II GTNNAC 4 cut(s) 481, 555, 595, 892
Hpy188I TCNGA 2 cut(s) 710, 939
Hpy188III TCNNGA 7 cut(s) 110, 171, 198, 508, 533, 539, 845
Hpy8I GTNNAC 4 cut(s) 481, 555, 595, 892
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 5 cut(s) 16, 61, 485, 655, 757
HpyCH4V TGCA 9 cut(s) 20, 71, 128, 251, 452, 548, 614, 830, 964
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 643
HpyF3I CTNAG 3 cut(s) 381, 699, 885
Hsp92II CATG 7 cut(s) 89, 187, 251, 479, 552, 848, 964
HspAI GCGC 1 cut(s) 92
KpnI GGTACC 1 cut(s) 790
Ksp22I TGATCA 1 cut(s) 244
KspAI GTTAAC 1 cut(s) 892
Kzo9I GATC 5 cut(s) 244, 267, 277, 370, 535
LguI GCTCTTC 1 cut(s) 509
LmnI GCTCC 2 cut(s) 29, 902
Lsp1109I GCAGC 1 cut(s) 842
LweI GCATC 1 cut(s) 191
MaeIII GTNAC 3 cut(s) 10, 443, 490
MalI GATC 5 cut(s) 246, 269, 279, 372, 537
MboI GATC 5 cut(s) 244, 267, 277, 370, 535
MboII GAAGA 4 cut(s) 380, 436, 496, 742
MfeI CAATTG 1 cut(s) 623
MflI RGATCY 2 cut(s) 370, 535
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 1 cut(s) 637
MluCI AATT 6 cut(s) 101, 230, 453, 609, 623, 940
MluNI TGGCCA 1 cut(s) 637
MmeI TCCRAC 1 cut(s) 401
Mox20I TGGCCA 1 cut(s) 637
MscI TGGCCA 1 cut(s) 637
MseI TTAA 3 cut(s) 375, 575, 891
MslI CAYNNNNRTG 1 cut(s) 252
Msp20I TGGCCA 1 cut(s) 637
MspA1I CMGCKG 3 cut(s) 365, 698, 833
MspI CCGG 5 cut(s) 294, 315, 588, 780, 790
MspR9I CCNGG 7 cut(s) 46, 214, 294, 316, 588, 780, 781
MunI CAATTG 1 cut(s) 623
Mva1269I GAATGC 1 cut(s) 138
MvaI CCWGG 2 cut(s) 46, 214
MwoI GCNNNNNNNGC 2 cut(s) 201, 643
NciI CCSGG 5 cut(s) 294, 316, 588, 780, 781
NdeII GATC 5 cut(s) 244, 267, 277, 370, 535
NlaIII CATG 7 cut(s) 89, 187, 251, 479, 552, 848, 964
NlaIV GGNNCC 3 cut(s) 218, 788, 904
NmuCI GTSAC 2 cut(s) 443, 490
PagI TCATGA 1 cut(s) 844
PasI CCCWGGG 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 509
PctI GAATGC 1 cut(s) 138
PfeI GAWTC 5 cut(s) 307, 337, 666, 690, 793
PfoI TCCNGGA 1 cut(s) 586
PkrI GCNGC 2 cut(s) 824, 832
Psp6I CCWGG 2 cut(s) 44, 212
PspEI GGTNACC 2 cut(s) 443, 490
PspGI CCWGG 2 cut(s) 44, 212
PspN4I GGNNCC 3 cut(s) 218, 788, 904
PspPI GGNCC 2 cut(s) 217, 317
PsuI RGATCY 2 cut(s) 370, 535
PvuII CAGCTG 3 cut(s) 365, 698, 833
RsaI GTAC 3 cut(s) 302, 788, 837
RsaNI GTAC 3 cut(s) 301, 787, 836
RseI CAYNNNNRTG 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 509
SaqAI TTAA 3 cut(s) 375, 575, 891
SatI GCNGC 2 cut(s) 823, 831
Sau3AI GATC 5 cut(s) 244, 267, 277, 370, 535
Sau96I GGNCC 2 cut(s) 217, 317
ScrFI CCNGG 7 cut(s) 46, 214, 294, 316, 588, 780, 781
SduI GDGCHC 1 cut(s) 780
SfaNI GCATC 1 cut(s) 191
SmaI CCCGGG 1 cut(s) 781
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 169, 531
SmoI CTYRAG 2 cut(s) 169, 531
Sse9I AATT 6 cut(s) 101, 230, 453, 609, 623, 940
SsiI CCGC 1 cut(s) 822
SspI AATATT 1 cut(s) 746
StyD4I CCNGG 7 cut(s) 44, 212, 292, 314, 586, 778, 779
StyI CCWWGG 1 cut(s) 898
TaaI ACNGT 5 cut(s) 16, 61, 485, 655, 757
TaqI TCGA 4 cut(s) 199, 270, 280, 801
TasI AATT 6 cut(s) 101, 230, 453, 609, 623, 940
TatI WGTACW 1 cut(s) 835
TauI GCSGC 1 cut(s) 825
TfiI GAWTC 5 cut(s) 307, 337, 666, 690, 793
Tru1I TTAA 3 cut(s) 375, 575, 891
Tru9I TTAA 3 cut(s) 375, 575, 891
TscAI CASTG 6 cut(s) 66, 197, 347, 488, 550, 679
TseFI GTSAC 2 cut(s) 443, 490
TseI GCWGC 1 cut(s) 830
Tsp45I GTSAC 2 cut(s) 443, 490
TspDTI ATGAA 5 cut(s) 102, 821, 833, 878, 927
TspMI CCCGGG 1 cut(s) 779
TspRI CASTG 6 cut(s) 66, 197, 347, 488, 550, 679
XapI RAATTY 1 cut(s) 230
XcmI CCANNNNNNNNNTGG 1 cut(s) 327
XmaI CCCGGG 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.