pycom01g10810

Beclin-1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005026
Physical Location & Seq
Reverse (-)
11605451 .. 11608134
2684 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
ATGGGCGAAAGGGGTCGGAGCTTTCAGGTGGATCCGAACGTCCCAAGATATGCATGCCAGAACTGCCGTAACTATCTCTGCGTCGTCGGCGCCGATTCCTACGCCGAACAATTCTTCCACGATTTCTCTCGCTCCGGCATGCAGGATGAGTCCTTTGTGGTTGTTTATAAATCTGAGTCCTCAGCGGATGGTGGTGGGGTCCATTTGCCGTTACCTGATGCAGGACCCAATGGTCCTTTGCAGCCTAACAATTCTGGGTTTCACTCTACTATAACTCTCCTGGATCGTGCATTTGAAATTGCCACCACCCAAACACTGGTTGAGCAACCTTTATGCATTGAATGCATGAGGGTATTGTTTGATAAACTTGACAAGGAGGTTGAAGATGTGAATAGGGACATTAAAGCATACGAAGCCTGTCTTAAACGCTTGGAGGGTGAGACAAGGGAAGTGCTTGGTGAGACTGATTTTCTCAAGGGAAAACTGAAGATTGAGGAAGAGGAGAGAAAACTTGAAGCAGCAATTGAAGAGATGGAGAAACAGAATGCAGAAGTAAATAATGAACTCAAGGGACTTGAGTTAAAATCTAGCAGATTTAAGGAGTTGGAGGAGCGGTACTGGCACGAGTTCAATATTTTTCAGTTTCAATTAATTTTGAATCAGGAAAAAAGAGATGCAATTTTGGCCAAAATAGAAGTTTCACAAGCACACTTAGAGTTGTTGAAATGA

Protein Analysis

243

Amino Acids

27.74

Weight (kDa)

4.88

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
APG6_N PF17675 110 - 238 9e-28 Apg6 coiled-coil region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 168
AasI GACNNNNNNGTC 1 cut(s) 231
AccB1I GGYRCC 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 316
AccBSI CCGCTC 1 cut(s) 613
AciI CCGC 2 cut(s) 185, 613
AclWI GGATC 3 cut(s) 26, 39, 291
AcoI YGGCCR 1 cut(s) 684
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 2 cut(s) 221, 316
AgsI TTSAA 9 cut(s) 296, 341, 383, 515, 527, 631, 647, 658, 724
AjnI CCWGG 1 cut(s) 279
AloI GAACNNNNNNTCC 2 cut(s) 99, 131
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw26I GTCTC 2 cut(s) 434, 455
AlwI GGATC 3 cut(s) 26, 39, 291
AoxI GGCC 1 cut(s) 684
ApeKI GCWGC 2 cut(s) 241, 518
AseI ATTAAT 1 cut(s) 650
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 3 cut(s) 199, 224, 233
AsuHPI GGTGA 2 cut(s) 449, 470
AvaII GGWCC 3 cut(s) 199, 224, 233
BalI TGGCCA 1 cut(s) 686
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 89
BauI CACGAG 1 cut(s) 623
BbvCI CCTCAGC 1 cut(s) 181
BbvI GCAGC 2 cut(s) 253, 530
BccI CCATC 2 cut(s) 182, 526
BceAI ACGGC 2 cut(s) 51, 193
BciT130I CCWGG 1 cut(s) 281
BcoDI GTCTC 2 cut(s) 434, 455
BfaI CTAG 1 cut(s) 588
BfoI RGCGCY 1 cut(s) 93
BisI GCNGC 2 cut(s) 242, 519
BlsI GCNGC 2 cut(s) 243, 520
Bme1390I CCNGG 1 cut(s) 281
Bme18I GGWCC 3 cut(s) 199, 224, 233
BmgT120I GGNCC 3 cut(s) 199, 224, 233
BmiI GGNNCC 4 cut(s) 33, 91, 200, 226
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 2 cut(s) 208, 664
Bpu10I CCTNAGC 1 cut(s) 181
BpuEI CTTGAG 3 cut(s) 458, 551, 596
BsaHI GRCGYC 1 cut(s) 90
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 316
Bse1I ACTGG 2 cut(s) 321, 623
BseBI CCWGG 1 cut(s) 281
BseGI GGATG 2 cut(s) 151, 193
BseLI CCNNNNNNNGG 2 cut(s) 221, 316
BseMII CTCAG 2 cut(s) 165, 195
BseNI ACTGG 2 cut(s) 321, 623
BseRI GAGGAG 2 cut(s) 515, 623
BseXI GCAGC 2 cut(s) 253, 530
BshFI GGCC 1 cut(s) 686
BshNI GGYRCC 1 cut(s) 89
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 3 cut(s) 26, 410, 585
BslI CCNNNNNNNGG 2 cut(s) 221, 316
BsmAI GTCTC 2 cut(s) 434, 455
BsmFI GGGAC 3 cut(s) 26, 410, 585
BsmI GAATGC 2 cut(s) 347, 550
BsnI GGCC 1 cut(s) 686
Bsp143I GATC 2 cut(s) 31, 283
BspACI CCGC 2 cut(s) 185, 613
BspANI GGCC 1 cut(s) 686
BspCNI CTCAG 2 cut(s) 166, 194
BspLI GGNNCC 4 cut(s) 33, 91, 200, 226
BspPI GGATC 3 cut(s) 26, 39, 291
BspT107I GGYRCC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 613
BsrI ACTGG 2 cut(s) 321, 623
BssMI GATC 2 cut(s) 31, 283
BssNI GRCGYC 1 cut(s) 90
BssSI CACGAG 1 cut(s) 623
Bst2BI CACGAG 1 cut(s) 623
Bst2UI CCWGG 1 cut(s) 281
Bst6I CTCTTC 2 cut(s) 492, 522
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 342
BstC8I GCNNGC 2 cut(s) 55, 140
BstDEI CTNAG 3 cut(s) 174, 181, 712
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 151, 193
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 2 cut(s) 34, 286
BstMAI GTCTC 2 cut(s) 434, 455
BstMBI GATC 2 cut(s) 31, 283
BstMWI GCNNNNNNNGC 6 cut(s) 63, 87, 342, 413, 619, 683
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 2 cut(s) 57, 142
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 253, 530
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BsuRI GGCC 1 cut(s) 686
BtsCI GGATG 2 cut(s) 151, 193
BtsIMutI CAGTG 1 cut(s) 314
Cac8I GCNNGC 2 cut(s) 55, 140
CfoI GCGC 1 cut(s) 92
Cfr13I GGNCC 3 cut(s) 199, 224, 233
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 616
CviAII CATG 3 cut(s) 54, 139, 346
CviJI RGCY 4 cut(s) 21, 244, 416, 686
CviKI_1 RGCY 4 cut(s) 21, 244, 416, 686
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 3 cut(s) 174, 181, 712
DinI GGCGCC 1 cut(s) 91
DpnI GATC 2 cut(s) 33, 285
DpnII GATC 2 cut(s) 31, 283
DrdI GACNNNNNNGTC 1 cut(s) 231
DseDI GACNNNNNNGTC 1 cut(s) 231
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 2 cut(s) 492, 522
EarI CTCTTC 2 cut(s) 492, 522
Eco47I GGWCC 3 cut(s) 199, 224, 233
Eco57I CTGAAG 1 cut(s) 506
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoO109I RGGNCCY 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 279
EcoT22I ATGCAT 3 cut(s) 55, 338, 347
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
FaeI CATG 3 cut(s) 57, 142, 349
FaiI YATR 8 cut(s) 51, 55, 140, 168, 272, 334, 347, 409
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 3 cut(s) 26, 410, 585
FatI CATG 3 cut(s) 53, 138, 345
Fnu4HI GCNGC 2 cut(s) 242, 519
FokI GGATG 2 cut(s) 158, 200
Fsp4HI GCNGC 2 cut(s) 242, 519
FspBI CTAG 1 cut(s) 588
GlaI GCGC 1 cut(s) 91
GluI GCNGC 2 cut(s) 242, 519
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 1 cut(s) 686
HapII CCGG 1 cut(s) 135
HgaI GACGC 1 cut(s) 70
HhaI GCGC 1 cut(s) 92
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 3 cut(s) 57, 142, 349
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HinfI GANTC 4 cut(s) 95, 149, 176, 658
HpaII CCGG 1 cut(s) 135
HphI GGTGA 2 cut(s) 449, 470
Hpy188I TCNGA 3 cut(s) 18, 36, 175
Hpy188III TCNNGA 1 cut(s) 662
Hpy99I CGWCG 2 cut(s) 86, 89
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 9 cut(s) 53, 142, 221, 241, 290, 336, 345, 548, 677
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 87, 342, 413, 619, 683
HpyF3I CTNAG 3 cut(s) 174, 181, 712
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 3 cut(s) 57, 142, 349
HspAI GCGC 1 cut(s) 90
KasI GGCGCC 1 cut(s) 89
Kzo9I GATC 2 cut(s) 31, 283
LmnI GCTCC 3 cut(s) 18, 137, 610
Lsp1109I GCAGC 2 cut(s) 253, 530
LweI GCATC 2 cut(s) 208, 664
MaeI CTAG 1 cut(s) 588
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 2 cut(s) 68, 210
MalI GATC 2 cut(s) 33, 285
MbiI CCGCTC 1 cut(s) 613
MboI GATC 2 cut(s) 31, 283
MboII GAAGA 5 cut(s) 106, 395, 499, 509, 539
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 686
MluCI AATT 7 cut(s) 110, 250, 297, 522, 647, 651, 678
MluNI TGGCCA 1 cut(s) 686
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 2 cut(s) 158, 185
MmeI TCCRAC 1 cut(s) 585
MnlI CCTC 7 cut(s) 190, 342, 370, 427, 487, 493, 601
Mox20I TGGCCA 1 cut(s) 686
Mph1103I ATGCAT 3 cut(s) 55, 338, 347
MscI TGGCCA 1 cut(s) 686
MseI TTAA 5 cut(s) 402, 423, 581, 597, 650
Msp20I TGGCCA 1 cut(s) 686
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 1 cut(s) 281
MunI CAATTG 1 cut(s) 522
Mva1269I GAATGC 2 cut(s) 347, 550
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 6 cut(s) 63, 87, 342, 413, 619, 683
NarI GGCGCC 1 cut(s) 90
NdeII GATC 2 cut(s) 31, 283
NlaIII CATG 3 cut(s) 57, 142, 349
NlaIV GGNNCC 4 cut(s) 33, 91, 200, 226
NsiI ATGCAT 3 cut(s) 55, 338, 347
NspI RCATGY 2 cut(s) 57, 142
PaeI GCATGC 2 cut(s) 57, 142
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 2 cut(s) 347, 550
PfeI GAWTC 2 cut(s) 95, 658
PflMI CCANNNNNTGG 1 cut(s) 316
PfoI TCCNGGA 1 cut(s) 279
PkrI GCNGC 2 cut(s) 243, 520
PleI GAGTC 2 cut(s) 157, 184
PluTI GGCGCC 1 cut(s) 93
PpsI GAGTC 2 cut(s) 157, 184
PpuMI RGGWCCY 1 cut(s) 224
PshBI ATTAAT 1 cut(s) 650
PsiI TTATAA 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspN4I GGNNCC 4 cut(s) 33, 91, 200, 226
PspPI GGNCC 3 cut(s) 199, 224, 233
PspPPI RGGWCCY 1 cut(s) 224
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
SaqAI TTAA 5 cut(s) 402, 423, 581, 597, 650
SatI GCNGC 2 cut(s) 242, 519
Sau3AI GATC 2 cut(s) 31, 283
Sau96I GGNCC 3 cut(s) 199, 224, 233
SchI GAGTC 2 cut(s) 158, 185
ScrFI CCNGG 1 cut(s) 281
SetI ASST 6 cut(s) 23, 30, 42, 217, 331, 381
SfaNI GCATC 2 cut(s) 208, 664
SfoI GGCGCC 1 cut(s) 91
SinI GGWCC 3 cut(s) 199, 224, 233
SmlI CTYRAG 3 cut(s) 473, 566, 575
SmoI CTYRAG 3 cut(s) 473, 566, 575
SphI GCATGC 2 cut(s) 57, 142
Sse9I AATT 7 cut(s) 110, 250, 297, 522, 647, 651, 678
SsiI CCGC 2 cut(s) 185, 613
SspDI GGCGCC 1 cut(s) 89
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 588
StyD4I CCNGG 1 cut(s) 279
TaiI ACGT 1 cut(s) 42
TasI AATT 7 cut(s) 110, 250, 297, 522, 647, 651, 678
TfiI GAWTC 2 cut(s) 95, 658
Tru1I TTAA 5 cut(s) 402, 423, 581, 597, 650
Tru9I TTAA 5 cut(s) 402, 423, 581, 597, 650
TscAI CASTG 1 cut(s) 321
TseI GCWGC 2 cut(s) 241, 518
TspDTI ATGAA 1 cut(s) 576
TspRI CASTG 1 cut(s) 321
Van91I CCANNNNNTGG 1 cut(s) 316
VpaK11BI GGWCC 3 cut(s) 199, 224, 233
VspI ATTAAT 1 cut(s) 650
XagI CCTNNNNNAGG 1 cut(s) 219
XceI RCATGY 2 cut(s) 57, 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XspI CTAG 1 cut(s) 588
Zsp2I ATGCAT 3 cut(s) 55, 338, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.