pycom01g13260

Tyramine N-feruloyltransferase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0012113
Physical Location & Seq
Reverse (-)
13763057 .. 13763758
702 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g13260.1

Sequence Viewer

Length: 702 bp
ATGGCCGCCGCCGCACCGCCACCGCCACCTACCCCAGCCCCAGAGGGCTTCAACGACGTACCCCGAGGCTCCACCCCAATGGGCTACCCACTCTTCTCTCGGATCCGCCTAGCCCACCCCACCGACGTCCCACACATCCACAAGCTCATCCACCAGATGGCCGTCTTCGAGCACCTCACCGACCTCTTCGTCGCCACCGAGTCTTCCCTCTCGTCCACCCTCTTCACCTCCCCTCCCTTCCAGTCATTCACCATCTTCGTCCTCGAAGTCTCTGAGACGCCTTTCGTCGAACACCTTCAGTGCAACAACTCGGCCTACCCACCCACCGTCAAAACCCACAATCTGGACCTCCCGATCGACGACCCAGAACGTGATTTGTTCAGATCCAACGGCGGGGACGCTGTCGTTGCTGGGTTTGTGCTGTTTTTCCCAAATTACTCCACCTTTTTGGGGAAGCCAGGGTTTTACATAGAGGACCTGTTTGTGAGGGAGTGCTATAGGAGGAAGGGGCTGGGGAAGATGCTGCTCTCAGCGGTGGCGAAGCAGGCGGTGAAGATGGGGTACGGCAGAGTGGAGTGGGTGGTGCTGGACTGGAACGTCAATGCCATCAAGTTTTACGAGGAGATGGGGGCTAAGGTTTTGCAGGAGTGGAGGATTTGCAGGCTCACTGGTGACGCTCTCAATGCTTATGTCAATGCCTAA

Protein Analysis

234

Amino Acids

26.05

Weight (kDa)

5.96

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acetyltransf_1 PF00583 116 - 210 5.7e-17 Acetyltransferase (GNAT) family
Acetyltransf_7 PF13508 136 - 210 3.5e-09 Acetyltransferase (GNAT) domain
FR47 PF08445 152 - 214 7e-06 FR47-like protein
Acetyltransf_10 PF13673 155 - 210 2.3e-07 Acetyltransferase (GNAT) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39020 AT2G39030
fragaria_vesca FvH4_7g19050
malus_domestica MD01G1105800.v1.1
prunus_persica Prupe.2G211200_v2.0.a1 Prupe.2G211200_v2.0.a1 Prupe.2G211200_v2.0.a1
pyrus_communis pycom01g13260
rosa_chinensis RchiOBHm_Chr1g0362951
rosa_laevigata RLG00000027608
rosa_multiflora Rmu_sc0000174.1_g000011 Rmu_sc0012461.1_g000003
rosa_roxburghii Rroxscaffold_4G00293610
rosa_rugosa Rorug01G0302300
rosa_samantha Rh1AG312000 Rh1BG275000 Rh1CG292300 Rh1DG305700
rosa_wichuraiana Rw1G027640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 188, 596
AatII GACGTC 1 cut(s) 129
AccB7I CCANNNNNTGG 2 cut(s) 157, 343
AciI CCGC 9 cut(s) 6, 9, 12, 17, 23, 106, 393, 533, 548
AclWI GGATC 3 cut(s) 97, 110, 378
AcoI YGGCCR 2 cut(s) 3, 159
AcuI CTGAAG 1 cut(s) 281
AcyI GRCGYC 2 cut(s) 126, 278
AfaI GTAC 2 cut(s) 60, 563
AfiI CCNNNNNNNGG 4 cut(s) 157, 343, 393, 450
AgsI TTSAA 1 cut(s) 52
AjnI CCWGG 1 cut(s) 457
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 2 cut(s) 269, 274
AlwI GGATC 3 cut(s) 97, 110, 378
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 3 cut(s) 3, 159, 312
ApeKI GCWGC 1 cut(s) 523
Asp700I GAANNNNTTC 1 cut(s) 294
AspS9I GGNCC 2 cut(s) 346, 475
AsuHPI GGTGA 5 cut(s) 169, 217, 241, 562, 683
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 346, 475
BamHI GGATCC 1 cut(s) 102
BarI GAAGNNNNNNTAC 2 cut(s) 545, 577
BbsI GAAGAC 2 cut(s) 157, 195
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 1 cut(s) 510
BccI CCATC 5 cut(s) 151, 260, 550, 614, 619
BceAI ACGGC 3 cut(s) 146, 406, 580
BciT130I CCWGG 1 cut(s) 459
BcoDI GTCTC 2 cut(s) 269, 274
BfaI CTAG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 496
BisI GCNGC 4 cut(s) 6, 9, 12, 524
BlsI GCNGC 4 cut(s) 7, 10, 13, 525
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 2 cut(s) 346, 475
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 346, 475
BmiI GGNNCC 2 cut(s) 70, 104
BmrFI CCNGG 1 cut(s) 459
BmsI GCATC 1 cut(s) 510
BpiI GAAGAC 2 cut(s) 157, 195
Bpu10I CCTNAGC 1 cut(s) 633
BsaHI GRCGYC 2 cut(s) 126, 278
BsaJI CCNNGG 2 cut(s) 64, 458
BsaXI ACNNNNNCTCC 2 cut(s) 217, 247
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 343, 393, 450
Bse1I ACTGG 3 cut(s) 241, 596, 673
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 2 cut(s) 64, 458
BseGI GGATG 2 cut(s) 135, 147
BseLI CCNNNNNNNGG 4 cut(s) 157, 343, 393, 450
BseMII CTCAG 2 cut(s) 264, 543
BseNI ACTGG 3 cut(s) 241, 596, 673
BseRI GAGGAG 1 cut(s) 635
BseXI GCAGC 1 cut(s) 510
BseYI CCCAGC 3 cut(s) 34, 410, 511
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 3 cut(s) 5, 161, 314
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 63
BslFI GGGAC 2 cut(s) 113, 410
BslI CCNNNNNNNGG 4 cut(s) 157, 343, 393, 450
BsmAI GTCTC 2 cut(s) 269, 274
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 113, 410
BsnI GGCC 3 cut(s) 5, 161, 314
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 3 cut(s) 102, 354, 383
BspACI CCGC 9 cut(s) 6, 9, 12, 17, 23, 106, 393, 533, 548
BspANI GGCC 3 cut(s) 5, 161, 314
BspCNI CTCAG 2 cut(s) 265, 542
BspLI GGNNCC 2 cut(s) 70, 104
BspPI GGATC 3 cut(s) 97, 110, 378
BsrI ACTGG 3 cut(s) 241, 596, 673
BssECI CCNNGG 2 cut(s) 64, 458
BssMI GATC 3 cut(s) 102, 354, 383
BssNI GRCGYC 2 cut(s) 126, 278
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 3 cut(s) 98, 191, 227
BstACI GRCGYC 2 cut(s) 126, 278
BstC8I GCNNGC 2 cut(s) 546, 662
BstDEI CTNAG 3 cut(s) 273, 529, 633
BstF5I GGATG 2 cut(s) 135, 147
BstKTI GATC 3 cut(s) 105, 357, 386
BstMAI GTCTC 2 cut(s) 269, 274
BstMBI GATC 3 cut(s) 102, 354, 383
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 4 cut(s) 11, 407, 545, 683
BstNI CCWGG 1 cut(s) 459
BstSCI CCNGG 1 cut(s) 457
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 1 cut(s) 510
BstV2I GAAGAC 2 cut(s) 157, 195
BstX2I RGATCY 2 cut(s) 102, 383
BstXI CCANNNNNNTGG 2 cut(s) 79, 448
BstYI RGATCY 2 cut(s) 102, 383
BsuRI GGCC 3 cut(s) 5, 161, 314
BtsCI GGATG 2 cut(s) 135, 147
BtsIMutI CAGTG 2 cut(s) 305, 666
Cac8I GCNNGC 2 cut(s) 546, 662
Cfr13I GGNCC 2 cut(s) 346, 475
CseI GACGC 3 cut(s) 286, 407, 683
Csp6I GTAC 2 cut(s) 59, 562
CviQI GTAC 2 cut(s) 59, 562
DdeI CTNAG 3 cut(s) 273, 529, 633
DpnI GATC 3 cut(s) 104, 356, 385
DpnII GATC 3 cut(s) 102, 354, 383
DrdI GACNNNNNNGTC 2 cut(s) 188, 596
DseDI GACNNNNNNGTC 2 cut(s) 188, 596
EaeI YGGCCR 2 cut(s) 3, 159
Eam1104I CTCTTC 3 cut(s) 98, 191, 227
EarI CTCTTC 3 cut(s) 98, 191, 227
EciI GGCGGA 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 346, 475
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 1 cut(s) 63
EcoO109I RGGNCCY 1 cut(s) 475
EcoRII CCWGG 1 cut(s) 457
Esp3I CGTCTC 1 cut(s) 269
FaiI YATR 3 cut(s) 470, 498, 690
FaqI GGGAC 2 cut(s) 113, 410
FauI CCCGC 1 cut(s) 386
Fnu4HI GCNGC 4 cut(s) 6, 9, 12, 524
FokI GGATG 2 cut(s) 122, 134
Fsp4HI GCNGC 4 cut(s) 6, 9, 12, 524
FspBI CTAG 1 cut(s) 110
GluI GCNGC 4 cut(s) 6, 9, 12, 524
GsaI CCCAGC 3 cut(s) 38, 414, 515
HaeIII GGCC 3 cut(s) 5, 161, 314
HgaI GACGC 3 cut(s) 286, 407, 683
Hin1I GRCGYC 2 cut(s) 126, 278
HinfI GANTC 1 cut(s) 200
HphI GGTGA 5 cut(s) 169, 217, 241, 562, 683
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 3 cut(s) 102, 274, 383
Hpy188III TCNNGA 2 cut(s) 344, 352
Hpy8I GTNNAC 1 cut(s) 216
Hpy99I CGWCG 5 cut(s) 59, 128, 194, 290, 362
HpyAV CCTTC 3 cut(s) 247, 305, 499
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 4 cut(s) 57, 126, 370, 597
HpyCH4V TGCA 3 cut(s) 303, 643, 660
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 407, 545, 683
HpyF3I CTNAG 3 cut(s) 273, 529, 633
HpySE526I ACGT 4 cut(s) 57, 126, 370, 597
Hsp92I GRCGYC 2 cut(s) 126, 278
Kzo9I GATC 3 cut(s) 102, 354, 383
LmnI GCTCC 1 cut(s) 74
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 1 cut(s) 510
MaeI CTAG 1 cut(s) 110
MaeII ACGT 4 cut(s) 57, 126, 370, 597
MaeIII GTNAC 1 cut(s) 671
MalI GATC 3 cut(s) 104, 356, 385
MboI GATC 3 cut(s) 102, 354, 383
MboII GAAGA 8 cut(s) 85, 157, 178, 195, 214, 247, 529, 565
MflI RGATCY 2 cut(s) 102, 383
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 1 cut(s) 433
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 459
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 4 cut(s) 11, 407, 545, 683
NdeII GATC 3 cut(s) 102, 354, 383
NlaIV GGNNCC 2 cut(s) 70, 104
NmeAIII GCCGAG 1 cut(s) 290
NmuCI GTSAC 1 cut(s) 671
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 294
PflFI GACNNNGTC 1 cut(s) 401
PflMI CCANNNNNTGG 2 cut(s) 157, 343
PkrI GCNGC 4 cut(s) 7, 10, 13, 525
Ple19I CGATCG 1 cut(s) 357
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
PpuMI RGGWCCY 1 cut(s) 475
Psp5II RGGWCCY 1 cut(s) 475
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 3 cut(s) 34, 410, 511
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 70, 104
PspPI GGNCC 2 cut(s) 346, 475
PspPPI RGGWCCY 1 cut(s) 475
PsuI RGATCY 2 cut(s) 102, 383
PsyI GACNNNGTC 1 cut(s) 401
PvuI CGATCG 1 cut(s) 357
RsaI GTAC 2 cut(s) 60, 563
RsaNI GTAC 2 cut(s) 59, 562
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 4 cut(s) 6, 9, 12, 524
Sau3AI GATC 3 cut(s) 102, 354, 383
Sau96I GGNCC 2 cut(s) 346, 475
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 1 cut(s) 510
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 2 cut(s) 346, 475
SmiMI CAYNNNNRTG 1 cut(s) 77
Sse9I AATT 1 cut(s) 433
SsiI CCGC 9 cut(s) 6, 9, 12, 17, 23, 106, 393, 533, 548
SspMI CTAG 1 cut(s) 110
StyD4I CCNGG 1 cut(s) 457
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 4 cut(s) 60, 129, 373, 600
TaqI TCGA 4 cut(s) 168, 264, 288, 357
TasI AATT 1 cut(s) 433
TauI GCSGC 3 cut(s) 8, 11, 14
TscAI CASTG 2 cut(s) 305, 673
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 1 cut(s) 523
Tsp45I GTSAC 1 cut(s) 671
TspRI CASTG 2 cut(s) 305, 673
Tth111I GACNNNGTC 1 cut(s) 401
Van91I CCANNNNNTGG 2 cut(s) 157, 343
VpaK11BI GGWCC 2 cut(s) 346, 475
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 1 cut(s) 110
ZraI GACGTC 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.