pycom01g13990

Golgi to ER traffic protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0009398
Physical Location & Seq
Reverse (-)
14300749 .. 14305622
4874 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
ATGGGGTATGTAGATATCACACCCTTTTTTATATTAAGGGGAGTAGATGTAATTCAGCGTAACTACCAAACCCGGATTCGTTTTGTGTGTTTCGAGTTGGAGAGAGTACAACCTTTGCTTACTTTGAACTCGACGCTCCCAGAAATTGAGACCATCATCATGTCGCGGGAGAGACCCAAAAGAGGCGCATTGCCTCCCGCCCAAGAGAATATTCAGAAATTAGAGAAAGTTGTAGAGGAAGGTAATTTCTATGGAGCTCAACAAATGTACAAATCAATTAGTGCAAGATATGTAGCTGCTCAGAGGTTTTCCGAGGCTGTGGATATCCTTCAGTCTGGCGCATGGAACCAACTGAAACATGGGCAGGTTACTTGTGGTGCTGAGTTAGCTGCTTTGTTTGTGGAAACACTTGTCAAAGGAAGATTCCCTTATGACGATGAAACCTTAGTTCGTGTCAGGAAAATTTATAAAGCATTTCCTCGGATACCTGTGCCGCAACAGTTAGGGGATTTAGATGACATGCAACAACTGTCTGAAACACTTGGGGCAGCAAAAACATGTGTTGAGGGCTGCTCATCATTCCTAAAGGCTGCTCTTAAGTGGTCTAAGGAGTTTGGTGCACCCAGGAGTGGAGCTCCAGAAATACATGTTATGCTAGCGAACTACATATATTTTGAATCTCCTGAGGTGGACATGGTTAGAGTGTCACATCATTTTGTTAGAGGAAACAACCCCAAAGAGTTTGCTTCGACTTTAGTAAATTTTATGGGCAAGTGTTATCCAGGGGAAGATGATGTGGCCATTGCCCGAGCTATTTTAATGTATTTGTCAGTTGGTAATCTGAGAGATGCCAATATTCTCATGGATGAGATAAAGAAGCAAGTGGAGTCTAAGGAGCTTGACTTTCCCAAATCCGATTTGATCCAGTTCATCAATTTTCTTTTGCAAACGTTGTTGAGAGATGCTCTTCCGCTTTTTAATATGTTGAGAGCGAAATACAAATCAAGCTTAGACAGAGAACCGACATTTCATGAGTTGCTGGATGAAATTGCGGAGAAGTTTTATGGAGTACGACGCAGAAATCCACTGCAAGGGATGGGGATGTTCGGCGAGATTTTCAAGATGATGGGAGGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF410

Protein Analysis

380

Amino Acids

43.22

Weight (kDa)

6.57

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GET4 PF04190 101 - 373 1.6e-66 Golgi to ER traffic protein 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
Acc36I ACCTGC 1 cut(s) 355
AccII CGCG 1 cut(s) 166
AciI CCGC 5 cut(s) 166, 198, 494, 971, 1052
AclI AACGTT 1 cut(s) 950
AclWI GGATC 1 cut(s) 916
AcoI YGGCCR 1 cut(s) 798
AcsI RAATTY 2 cut(s) 462, 760
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 3 cut(s) 108, 269, 1071
AfiI CCNNNNNNNGG 3 cut(s) 182, 629, 1091
AflII CTTAAG 1 cut(s) 596
AflIII ACRYGT 2 cut(s) 557, 646
AgsI TTSAA 3 cut(s) 127, 677, 1120
AjnI CCWGG 2 cut(s) 623, 781
AjuI GAANNNNNNNTTGG 4 cut(s) 60, 92, 195, 227
AluBI AGCT 7 cut(s) 257, 296, 389, 635, 812, 898, 1008
AluI AGCT 7 cut(s) 257, 296, 389, 635, 812, 898, 1008
Alw21I GWGCWC 3 cut(s) 259, 622, 637
Alw26I GTCTC 2 cut(s) 143, 166
Alw44I GTGCAC 1 cut(s) 618
AlwI GGATC 1 cut(s) 916
Ama87I CYCGRG 1 cut(s) 807
AoxI GGCC 1 cut(s) 798
ApaLI GTGCAC 1 cut(s) 618
ApeKI GCWGC 5 cut(s) 296, 389, 548, 570, 590
ApoI RAATTY 2 cut(s) 462, 760
AspLEI GCGC 2 cut(s) 188, 341
AsuC2I CCSGG 1 cut(s) 73
AsuNHI GCTAGC 1 cut(s) 655
AvaI CYCGRG 1 cut(s) 807
AxyI CCTNAGG 1 cut(s) 684
BaeGI GKGCMC 1 cut(s) 622
BalI TGGCCA 1 cut(s) 800
BanII GRGCYC 2 cut(s) 259, 637
Bbv12I GWGCWC 3 cut(s) 259, 622, 637
BbvI GCAGC 5 cut(s) 283, 376, 557, 560, 577
BccI CCATC 3 cut(s) 161, 1090, 1120
BciT130I CCWGG 2 cut(s) 625, 783
BciVI GTATCC 1 cut(s) 477
BcnI CCSGG 1 cut(s) 73
BcoDI GTCTC 2 cut(s) 143, 166
BfaI CTAG 1 cut(s) 656
BfrI CTTAAG 1 cut(s) 596
BfuAI ACCTGC 1 cut(s) 355
BfuI GTATCC 1 cut(s) 477
BisI GCNGC 6 cut(s) 297, 390, 494, 549, 571, 591
BlsI GCNGC 6 cut(s) 298, 391, 495, 550, 572, 592
Bme1390I CCNGG 3 cut(s) 73, 625, 783
BmeT110I CYCGRG 1 cut(s) 807
BmiI GGNNCC 1 cut(s) 347
BmrFI CCNGG 3 cut(s) 73, 625, 783
BmsI GCATC 2 cut(s) 838, 952
BmtI GCTAGC 1 cut(s) 659
BplI GAGNNNNNCTC 6 cut(s) 557, 589, 619, 651, 949, 981
BpmI CTGGAG 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 73
BsaI GGTCTC 2 cut(s) 143, 166
BsaJI CCNNGG 4 cut(s) 312, 479, 623, 782
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 629, 1091
Bse1I ACTGG 1 cut(s) 925
Bse21I CCTNAGG 1 cut(s) 684
Bse3DI GCAATG 2 cut(s) 188, 801
BseBI CCWGG 2 cut(s) 625, 783
BseDI CCNNGG 4 cut(s) 312, 479, 623, 782
BseGI GGATG 4 cut(s) 871, 1048, 1101, 1107
BseLI CCNNNNNNNGG 3 cut(s) 182, 629, 1091
BseMI GCAATG 2 cut(s) 188, 801
BseMII CTCAG 4 cut(s) 314, 372, 675, 833
BseNI ACTGG 1 cut(s) 925
BseSI GKGCMC 1 cut(s) 622
BseXI GCAGC 5 cut(s) 283, 376, 557, 560, 577
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 1 cut(s) 800
BsiHKAI GWGCWC 3 cut(s) 259, 622, 637
BsiHKCI CYCGRG 1 cut(s) 807
BsiSI CCGG 1 cut(s) 73
BslI CCNNNNNNNGG 3 cut(s) 182, 629, 1091
BsmAI GTCTC 2 cut(s) 143, 166
BsnI GGCC 1 cut(s) 800
Bso31I GGTCTC 2 cut(s) 143, 166
BsoBI CYCGRG 1 cut(s) 807
Bsp1286I GDGCHC 3 cut(s) 259, 622, 637
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 1 cut(s) 921
BspACI CCGC 5 cut(s) 166, 198, 494, 971, 1052
BspANI GGCC 1 cut(s) 800
BspCNI CTCAG 4 cut(s) 313, 373, 676, 834
BspFNI CGCG 1 cut(s) 166
BspHI TCATGA 1 cut(s) 1030
BspLI GGNNCC 1 cut(s) 347
BspMI ACCTGC 1 cut(s) 355
BspOI GCTAGC 1 cut(s) 659
BspPI GGATC 1 cut(s) 916
BspQI GCTCTTC 1 cut(s) 972
BspTI CTTAAG 1 cut(s) 596
BspTNI GGTCTC 2 cut(s) 143, 166
BsrDI GCAATG 2 cut(s) 188, 801
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 1 cut(s) 925
BssECI CCNNGG 4 cut(s) 312, 479, 623, 782
BssMI GATC 1 cut(s) 921
Bst2UI CCWGG 2 cut(s) 625, 783
Bst4CI ACNGT 2 cut(s) 501, 531
Bst6I CTCTTC 1 cut(s) 972
BstAFI CTTAAG 1 cut(s) 596
BstAUI TGTACA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 8 cut(s) 300, 381, 445, 606, 684, 842, 891, 1009
BstF5I GGATG 4 cut(s) 871, 1048, 1101, 1107
BstFNI CGCG 1 cut(s) 166
BstHHI GCGC 2 cut(s) 188, 341
BstKTI GATC 1 cut(s) 924
BstMAI GTCTC 2 cut(s) 143, 166
BstMBI GATC 1 cut(s) 921
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 2 cut(s) 625, 783
BstNSI RCATGY 3 cut(s) 523, 561, 650
BstSCI CCNGG 3 cut(s) 71, 623, 781
BstSLI GKGCMC 1 cut(s) 622
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 5 cut(s) 283, 376, 557, 560, 577
Bsu36I CCTNAGG 1 cut(s) 684
BsuI GTATCC 1 cut(s) 477
BsuRI GGCC 1 cut(s) 800
BtsCI GGATG 4 cut(s) 871, 1048, 1101, 1107
BtsI GCAGTG 1 cut(s) 1085
BtsIMutI CAGTG 1 cut(s) 1085
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 1 cut(s) 657
CciI TCATGA 1 cut(s) 1030
CfoI GCGC 2 cut(s) 188, 341
CseI GACGC 2 cut(s) 142, 1083
Csp6I GTAC 3 cut(s) 107, 268, 1070
CviAII CATG 9 cut(s) 160, 342, 359, 520, 558, 647, 694, 862, 1031
CviQI GTAC 3 cut(s) 107, 268, 1070
DdeI CTNAG 8 cut(s) 300, 381, 445, 606, 684, 842, 891, 1009
DpnI GATC 1 cut(s) 923
DpnII GATC 1 cut(s) 921
EaeI YGGCCR 1 cut(s) 798
Eam1104I CTCTTC 1 cut(s) 972
EarI CTCTTC 1 cut(s) 972
Ecl136II GAGCTC 2 cut(s) 257, 635
Eco24I GRGCYC 2 cut(s) 259, 637
Eco31I GGTCTC 2 cut(s) 143, 166
Eco32I GATATC 2 cut(s) 16, 325
Eco53kI GAGCTC 2 cut(s) 257, 635
Eco57I CTGAAG 1 cut(s) 314
Eco81I CCTNAGG 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 807
EcoICRI GAGCTC 2 cut(s) 257, 635
EcoRII CCWGG 2 cut(s) 623, 781
EcoRV GATATC 2 cut(s) 16, 325
EcoT38I GRGCYC 2 cut(s) 259, 637
FaeI CATG 9 cut(s) 163, 345, 362, 523, 561, 650, 697, 865, 1034
FalI AAGNNNNNCTT 2 cut(s) 412, 444
FatI CATG 9 cut(s) 159, 341, 358, 519, 557, 646, 693, 861, 1030
FauI CCCGC 2 cut(s) 159, 205
Fnu4HI GCNGC 6 cut(s) 297, 390, 494, 549, 571, 591
FokI GGATG 4 cut(s) 878, 1055, 1108, 1114
FriOI GRGCYC 2 cut(s) 259, 637
Fsp4HI GCNGC 6 cut(s) 297, 390, 494, 549, 571, 591
FspBI CTAG 1 cut(s) 656
GlaI GCGC 2 cut(s) 187, 340
GluI GCNGC 6 cut(s) 297, 390, 494, 549, 571, 591
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 1 cut(s) 800
HapII CCGG 1 cut(s) 73
HgaI GACGC 2 cut(s) 142, 1083
HhaI GCGC 2 cut(s) 188, 341
Hin1II CATG 9 cut(s) 163, 345, 362, 523, 561, 650, 697, 865, 1034
Hin6I GCGC 2 cut(s) 186, 339
HinP1I GCGC 2 cut(s) 186, 339
HindIII AAGCTT 1 cut(s) 1006
HinfI GANTC 4 cut(s) 76, 423, 677, 887
HpaII CCGG 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 620, 691
Hpy188I TCNGA 7 cut(s) 216, 303, 313, 483, 535, 843, 916
Hpy188III TCNNGA 5 cut(s) 457, 638, 683, 1031, 1120
Hpy8I GTNNAC 2 cut(s) 620, 691
Hpy99I CGWCG 2 cut(s) 136, 1077
HpyAV CCTTC 2 cut(s) 233, 338
HpyCH4III ACNGT 2 cut(s) 501, 531
HpyCH4IV ACGT 1 cut(s) 950
HpyCH4V TGCA 5 cut(s) 284, 523, 620, 946, 1090
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 8 cut(s) 300, 381, 445, 606, 684, 842, 891, 1009
HpySE526I ACGT 1 cut(s) 950
Hsp92II CATG 9 cut(s) 163, 345, 362, 523, 561, 650, 697, 865, 1034
HspAI GCGC 2 cut(s) 186, 339
Kzo9I GATC 1 cut(s) 921
LguI GCTCTTC 1 cut(s) 972
LmnI GCTCC 5 cut(s) 141, 254, 632, 640, 895
Lsp1109I GCAGC 5 cut(s) 283, 376, 557, 560, 577
LweI GCATC 2 cut(s) 838, 952
MaeI CTAG 1 cut(s) 656
MaeII ACGT 1 cut(s) 950
MaeIII GTNAC 3 cut(s) 59, 367, 705
MalI GATC 1 cut(s) 923
MboI GATC 1 cut(s) 921
MboII GAAGA 3 cut(s) 432, 800, 959
MhlI GDGCHC 3 cut(s) 259, 622, 637
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 9 cut(s) 51, 144, 218, 244, 276, 462, 760, 934, 1047
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 1 cut(s) 896
MmeI TCCRAC 1 cut(s) 78
Mox20I TGGCCA 1 cut(s) 800
MscI TGGCCA 1 cut(s) 800
MseI TTAA 4 cut(s) 35, 597, 818, 978
MslI CAYNNNNRTG 1 cut(s) 158
Msp20I TGGCCA 1 cut(s) 800
MspCI CTTAAG 1 cut(s) 596
MspI CCGG 1 cut(s) 73
MspR9I CCNGG 3 cut(s) 73, 625, 783
MvaI CCWGG 2 cut(s) 625, 783
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 1 cut(s) 386
NciI CCSGG 1 cut(s) 73
NdeII GATC 1 cut(s) 921
NheI GCTAGC 1 cut(s) 655
NlaIII CATG 9 cut(s) 163, 345, 362, 523, 561, 650, 697, 865, 1034
NlaIV GGNNCC 1 cut(s) 347
NmuCI GTSAC 1 cut(s) 705
NspI RCATGY 3 cut(s) 523, 561, 650
PagI TCATGA 1 cut(s) 1030
PciI ACATGT 2 cut(s) 557, 646
PciSI GCTCTTC 1 cut(s) 972
PfeI GAWTC 3 cut(s) 76, 423, 677
PkrI GCNGC 6 cut(s) 298, 391, 495, 550, 572, 592
PleI GAGTC 1 cut(s) 895
PpsI GAGTC 1 cut(s) 895
PscI ACATGT 2 cut(s) 557, 646
PsiI TTATAA 1 cut(s) 468
Psp124BI GAGCTC 2 cut(s) 259, 637
Psp1406I AACGTT 1 cut(s) 950
Psp6I CCWGG 2 cut(s) 623, 781
PspGI CCWGG 2 cut(s) 623, 781
PspN4I GGNNCC 1 cut(s) 347
RsaI GTAC 3 cut(s) 108, 269, 1071
RsaNI GTAC 3 cut(s) 107, 268, 1070
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 2 cut(s) 259, 637
SapI GCTCTTC 1 cut(s) 972
SaqAI TTAA 4 cut(s) 35, 597, 818, 978
SatI GCNGC 6 cut(s) 297, 390, 494, 549, 571, 591
Sau3AI GATC 1 cut(s) 921
SchI GAGTC 1 cut(s) 896
ScrFI CCNGG 3 cut(s) 73, 625, 783
SduI GDGCHC 3 cut(s) 259, 622, 637
SfaNI GCATC 2 cut(s) 838, 952
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 596
SmoI CTYRAG 1 cut(s) 596
Sse9I AATT 9 cut(s) 51, 144, 218, 244, 276, 462, 760, 934, 1047
SsiI CCGC 5 cut(s) 166, 198, 494, 971, 1052
SspI AATATT 2 cut(s) 211, 856
SspMI CTAG 1 cut(s) 656
SstI GAGCTC 2 cut(s) 259, 637
StyD4I CCNGG 3 cut(s) 71, 623, 781
TaaI ACNGT 2 cut(s) 501, 531
TaiI ACGT 1 cut(s) 953
TaqI TCGA 3 cut(s) 93, 131, 749
TasI AATT 9 cut(s) 51, 144, 218, 244, 276, 462, 760, 934, 1047
TatI WGTACW 2 cut(s) 106, 267
TauI GCSGC 1 cut(s) 496
TfiI GAWTC 3 cut(s) 76, 423, 677
Tru1I TTAA 4 cut(s) 35, 597, 818, 978
Tru9I TTAA 4 cut(s) 35, 597, 818, 978
TscAI CASTG 1 cut(s) 1092
TseFI GTSAC 1 cut(s) 705
TseI GCWGC 5 cut(s) 296, 389, 548, 570, 590
Tsp45I GTSAC 1 cut(s) 705
TspDTI ATGAA 4 cut(s) 453, 919, 1019, 1059
TspRI CASTG 1 cut(s) 1092
Vha464I CTTAAG 1 cut(s) 596
VneI GTGCAC 1 cut(s) 618
XapI RAATTY 2 cut(s) 462, 760
XceI RCATGY 3 cut(s) 523, 561, 650
XcmI CCANNNNNNNNNTGG 2 cut(s) 356, 859
XspI CTAG 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.