pycom01g15670

Ribosomal RNA large subunit methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011170
Physical Location & Seq
Forward (+)
15450649 .. 15452163
1515 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGATAAAACTTTCCTCGTGGGGCTGGAAAACCAGATTTATTCTACAGAGAAGCTCAACGCCTGGGTTATGTGGCTCGCTCAGCCTTCAAGCTCCTTCAGATGCAGAAGCAGTACAAGCTCATAACCCCTGGCTCCTCTGTTCTCGACCTCGGCTGTGCTCCCGGCGCTTGGCTTCAGGTGGCTTGCCAGAGTTTGGGTCCCCTAAAAAATGGCGGCCTTGTTGTCGGATATTCCGATTGATCTCAAGGTTCCTGCTCGATCATTGTGATTCAAGGGTTCAAACTGTTTGCGCCGAGAGCTCAAGTCAGGGAACTCTCTCCGCAGGATGTTTGAAGAAAGCGTGTTCTGTGGTGCTGTTGGATATGTGTCCCCTCGTTTCTGGAATCTCAAGTAAAGATGCAGCTTTATCGCTTGAGTTAGGAATGCAAGCACTGAATTTGGCTGTTGGTGGAGCAGCCTTCTCATTCAAACAACGAAATTCAAACGGAGGAAAGGGAGGCAATCTTGTGATAAAGCTTTTAGAGAGTGAAGATGTGCAAGCGGTAATTGCTTCATTGAATCTCTGTTTGGATATATTTCTTTACCATCTTCTGGCTGCTTGTTTTGCAGAAATCAATCAGATTTGCAAACCGCTGTTCAGAAAGGCGTCATTGTTGAGGCCTAAAGCTACAAGATCGATCATCGAGAGAGATTTATTTGATCTGTCAAGGTCTGCAGTCACAGGCAAAGACATAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FtsJ

Protein Analysis

247

Amino Acids

26.73

Weight (kDa)

9.44

Isoelectric Point (pI)

48.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FtsJ PF01728 108 - 191 4.2e-06 FtsJ-like methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13830
fragaria_vesca FvH4_7g22770
malus_domestica MD07G1200600.v1.1
prunus_persica Prupe.2G237400_v2.0.a1
pyrus_communis pycom01g15670 pycom07g18760 pycom07g18770
rosa_chinensis RchiOBHm_Chr1g0367871
rosa_laevigata RLG00000027260
rosa_multiflora Rmu_co8050104.1_g000001 Rmu_sc0010008.1_g000001 Rmu_ssc0000395.1_g000039
rosa_roxburghii Rroxscaffold_4G00289170
rosa_rugosa Rorug01G0337300 Rorug01G0337400
rosa_samantha Rh1AG344200 Rh1BG306200 Rh1CG321800 Rh1DG338400
rosa_wichuraiana Rw1G030640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 194, 592
AciI CCGC 4 cut(s) 214, 321, 542, 632
AcsI RAATTY 2 cut(s) 436, 478
AcuI CTGAAG 2 cut(s) 81, 159
AcyI GRCGYC 1 cut(s) 647
AfaI GTAC 1 cut(s) 114
AfiI CCNNNNNNNGG 3 cut(s) 169, 194, 592
AgsI TTSAA 7 cut(s) 89, 273, 281, 334, 469, 483, 559
AjnI CCWGG 2 cut(s) 61, 128
AjuI GAANNNNNNNTTGG 2 cut(s) 551, 583
AluBI AGCT 7 cut(s) 54, 92, 119, 300, 404, 517, 668
AluI AGCT 7 cut(s) 54, 92, 119, 300, 404, 517, 668
Alw21I GWGCWC 2 cut(s) 161, 302
AoxI GGCC 2 cut(s) 215, 659
ApeKI GCWGC 3 cut(s) 401, 455, 596
ApoI RAATTY 2 cut(s) 436, 478
AspLEI GCGC 2 cut(s) 168, 293
AspS9I GGNCC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 163
AvaII GGWCC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 302
BauI CACGAG 1 cut(s) 16
Bbv12I GWGCWC 2 cut(s) 161, 302
BbvI GCAGC 3 cut(s) 413, 467, 583
BccI CCATC 1 cut(s) 594
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciT130I CCWGG 2 cut(s) 63, 130
BcnI CCSGG 1 cut(s) 163
BfmI CTRYAG 2 cut(s) 44, 714
BfoI RGCGCY 1 cut(s) 169
BisI GCNGC 4 cut(s) 215, 402, 456, 597
BlpI GCTNAGC 1 cut(s) 80
BlsI GCNGC 4 cut(s) 216, 403, 457, 598
Bme1390I CCNGG 3 cut(s) 63, 130, 163
Bme18I GGWCC 1 cut(s) 198
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 4 cut(s) 134, 199, 200, 251
BmrFI CCNGG 3 cut(s) 63, 130, 163
BmsI GCATC 2 cut(s) 91, 388
Bpu1102I GCTNAGC 1 cut(s) 80
BpuEI CTTGAG 4 cut(s) 229, 286, 373, 434
BpuMI CCSGG 1 cut(s) 163
Bsa29I ATCGAT 1 cut(s) 677
BsaHI GRCGYC 1 cut(s) 647
BsaJI CCNNGG 3 cut(s) 62, 128, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 169, 194, 592
BseBI CCWGG 2 cut(s) 63, 130
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 3 cut(s) 62, 128, 149
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 3 cut(s) 169, 194, 592
BseMII CTCAG 1 cut(s) 94
BseRI GAGGAG 1 cut(s) 125
BseXI GCAGC 3 cut(s) 413, 467, 583
BshFI GGCC 2 cut(s) 217, 661
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 2 cut(s) 161, 302
BsiSI CCGG 1 cut(s) 163
BslFI GGGAC 2 cut(s) 184, 354
BslI CCNNNNNNNGG 3 cut(s) 169, 194, 592
BsmFI GGGAC 2 cut(s) 184, 354
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 2 cut(s) 217, 661
Bsp1286I GDGCHC 2 cut(s) 161, 302
Bsp143I GATC 5 cut(s) 240, 259, 674, 678, 700
Bsp1720I GCTNAGC 1 cut(s) 80
BspACI CCGC 4 cut(s) 214, 321, 542, 632
BspANI GGCC 2 cut(s) 217, 661
BspCNI CTCAG 1 cut(s) 93
BspDI ATCGAT 1 cut(s) 677
BspLI GGNNCC 4 cut(s) 134, 199, 200, 251
BspMAI CTGCAG 1 cut(s) 718
BssECI CCNNGG 3 cut(s) 62, 128, 149
BssMI GATC 5 cut(s) 240, 259, 674, 678, 700
BssNI GRCGYC 1 cut(s) 647
BssSI CACGAG 1 cut(s) 16
Bst2BI CACGAG 1 cut(s) 16
Bst2UI CCWGG 2 cut(s) 63, 130
Bst4CI ACNGT 1 cut(s) 286
BstACI GRCGYC 1 cut(s) 647
BstC8I GCNNGC 4 cut(s) 77, 185, 429, 540
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 1 cut(s) 332
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 2 cut(s) 168, 293
BstKTI GATC 5 cut(s) 243, 262, 677, 681, 703
BstMBI GATC 5 cut(s) 240, 259, 674, 678, 700
BstMWI GCNNNNNNNGC 6 cut(s) 81, 116, 165, 297, 548, 605
BstNI CCWGG 2 cut(s) 63, 130
BstSCI CCNGG 3 cut(s) 61, 128, 161
BstSFI CTRYAG 2 cut(s) 44, 714
BstV1I GCAGC 3 cut(s) 413, 467, 583
Bsu15I ATCGAT 1 cut(s) 677
BsuRI GGCC 2 cut(s) 217, 661
BsuTUI ATCGAT 1 cut(s) 677
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 1 cut(s) 431
Cac8I GCNNGC 4 cut(s) 77, 185, 429, 540
CfoI GCGC 2 cut(s) 168, 293
Cfr13I GGNCC 1 cut(s) 198
ClaI ATCGAT 1 cut(s) 677
CseI GACGC 1 cut(s) 636
Csp6I GTAC 1 cut(s) 113
CviQI GTAC 1 cut(s) 113
DdeI CTNAG 1 cut(s) 80
DpnI GATC 5 cut(s) 242, 261, 676, 680, 702
DpnII GATC 5 cut(s) 240, 259, 674, 678, 700
Ecl136II GAGCTC 1 cut(s) 300
Eco147I AGGCCT 1 cut(s) 661
Eco24I GRGCYC 1 cut(s) 302
Eco47I GGWCC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 300
Eco57I CTGAAG 2 cut(s) 81, 159
EcoICRI GAGCTC 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 61, 128
EcoT38I GRGCYC 1 cut(s) 302
FaiI YATR 5 cut(s) 70, 123, 365, 575, 734
FaqI GGGAC 2 cut(s) 184, 354
Fnu4HI GCNGC 4 cut(s) 215, 402, 456, 597
FokI GGATG 1 cut(s) 339
FriOI GRGCYC 1 cut(s) 302
Fsp4HI GCNGC 4 cut(s) 215, 402, 456, 597
GlaI GCGC 2 cut(s) 167, 292
GluI GCNGC 4 cut(s) 215, 402, 456, 597
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 217, 661
HapII CCGG 1 cut(s) 163
HgaI GACGC 1 cut(s) 636
HhaI GCGC 2 cut(s) 168, 293
Hin1I GRCGYC 1 cut(s) 647
Hin6I GCGC 2 cut(s) 166, 291
HinP1I GCGC 2 cut(s) 166, 291
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 3 cut(s) 269, 384, 559
HpaII CCGG 1 cut(s) 163
Hpy188I TCNGA 5 cut(s) 100, 228, 236, 621, 641
Hpy188III TCNNGA 3 cut(s) 144, 381, 685
HpyAV CCTTC 3 cut(s) 95, 105, 469
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 7 cut(s) 104, 401, 427, 538, 608, 627, 716
HpyF10VI GCNNNNNNNGC 6 cut(s) 81, 116, 165, 297, 548, 605
HpyF3I CTNAG 1 cut(s) 80
Hsp92I GRCGYC 1 cut(s) 647
HspAI GCGC 2 cut(s) 166, 291
KflI GGGWCCC 1 cut(s) 198
Kzo9I GATC 5 cut(s) 240, 259, 674, 678, 700
LmnI GCTCC 4 cut(s) 97, 138, 164, 452
Lsp1109I GCAGC 3 cut(s) 413, 467, 583
LweI GCATC 2 cut(s) 91, 388
MaeIII GTNAC 1 cut(s) 718
MalI GATC 5 cut(s) 242, 261, 676, 680, 702
MboI GATC 5 cut(s) 240, 259, 674, 678, 700
MboII GAAGA 3 cut(s) 346, 542, 581
MhlI GDGCHC 2 cut(s) 161, 302
MluCI AATT 3 cut(s) 436, 478, 546
MmeI TCCRAC 2 cut(s) 206, 339
MnlI CCTC 7 cut(s) 25, 146, 159, 383, 482, 491, 651
MspA1I CMGCKG 1 cut(s) 634
MspI CCGG 1 cut(s) 163
MspR9I CCNGG 3 cut(s) 63, 130, 163
Mva1269I GAATGC 1 cut(s) 429
MvaI CCWGG 2 cut(s) 63, 130
MwoI GCNNNNNNNGC 6 cut(s) 81, 116, 165, 297, 548, 605
NciI CCSGG 1 cut(s) 163
NdeII GATC 5 cut(s) 240, 259, 674, 678, 700
NlaIV GGNNCC 4 cut(s) 134, 199, 200, 251
NmeAIII GCCGAG 2 cut(s) 130, 319
NmuCI GTSAC 1 cut(s) 718
PceI AGGCCT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PctI GAATGC 1 cut(s) 429
PfeI GAWTC 3 cut(s) 269, 384, 559
PflMI CCANNNNNTGG 2 cut(s) 194, 592
PkrI GCNGC 4 cut(s) 216, 403, 457, 598
PpuMI RGGWCCY 1 cut(s) 198
Psp124BI GAGCTC 1 cut(s) 302
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 2 cut(s) 61, 128
PspGI CCWGG 2 cut(s) 61, 128
PspN4I GGNNCC 4 cut(s) 134, 199, 200, 251
PspPI GGNCC 1 cut(s) 198
PspPPI RGGWCCY 1 cut(s) 198
PstI CTGCAG 1 cut(s) 718
RsaI GTAC 1 cut(s) 114
RsaNI GTAC 1 cut(s) 113
SacI GAGCTC 1 cut(s) 302
SatI GCNGC 4 cut(s) 215, 402, 456, 597
Sau3AI GATC 5 cut(s) 240, 259, 674, 678, 700
Sau96I GGNCC 1 cut(s) 198
ScrFI CCNGG 3 cut(s) 63, 130, 163
SduI GDGCHC 2 cut(s) 161, 302
SfaNI GCATC 2 cut(s) 91, 388
SfcI CTRYAG 2 cut(s) 44, 714
SinI GGWCC 1 cut(s) 198
SmlI CTYRAG 4 cut(s) 244, 301, 388, 413
SmoI CTYRAG 4 cut(s) 244, 301, 388, 413
Sse9I AATT 3 cut(s) 436, 478, 546
SseBI AGGCCT 1 cut(s) 661
SsiI CCGC 4 cut(s) 214, 321, 542, 632
SstI GAGCTC 1 cut(s) 302
StuI AGGCCT 1 cut(s) 661
StyD4I CCNGG 3 cut(s) 61, 128, 161
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 4 cut(s) 145, 258, 677, 684
TasI AATT 3 cut(s) 436, 478, 546
TatI WGTACW 1 cut(s) 112
TauI GCSGC 1 cut(s) 217
TfiI GAWTC 3 cut(s) 269, 384, 559
TscAI CASTG 1 cut(s) 438
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 3 cut(s) 401, 455, 596
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 1 cut(s) 543
TspGWI ACGGA 1 cut(s) 501
TspRI CASTG 1 cut(s) 438
Van91I CCANNNNNTGG 2 cut(s) 194, 592
VpaK11BI GGWCC 1 cut(s) 198
XapI RAATTY 2 cut(s) 436, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.