pycom01g17270

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0014271
Physical Location & Seq
Reverse (-)
16610111 .. 16611008
898 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGGAAGAAGACCAACACCGCGATTCGGCAGCTGAAGCAGCACCAGATCATCACCTTCCCCAAGACAACAGCAACAGCAACAAAACCACTGAGGACAACTCTACCACCGCTTCCACCGCCACAACAGAAATAACCAACAACAACAACAGAAAGTGCAAGGGCAAAGGAGGCCCTGACAACAACAAGTTCAGGTACCGCGGCGTGCGCCAGCGGAGCTGGGGCAAATGGGTGGCTGAGATCCGTGAGCCGCGTAAGCGCACGCGCAAGTGGCTCGGGACCTTCGCCACTGCCGAGGACGCTGCCCACGCCTACGACCGGGCCGCCATCATCCTTTACGGTTCCAGGGCCCAGCTTAATCTCCAGCCCTCCGGTTCCTCGTCCCAGAACTCCACGCGGGACCGCTTCTCCCCCGCCCGTCGGGCTTCGGCCTTACTCTGCCCTACCCCTCCTCTGCCCACCCGGTCCCGCTCATGGATTCGGGGTTCGTTCCATACGGTGTTGATCAAGATCATGAGTATCAAAACAACATTAATCCTTTGCACCAACAACAACAGCAACATCAGCAGCAACAAATTGTGGTGCAACAATTTCATCATCAGTACCCCATCGCATTATCCGACGGCAGCGGTTGTGACACGTCAACCTCGTATCTACATCCAAACCCTAGTCATGATCAATACCAACGACAAGTACCCCACCAGAATAACAATAATCAGGAGTGCTGTTCGTACGAGGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000272 GO:0001067 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0006355 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009755 GO:0009756 GO:0009791 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009987 GO:0010033 GO:0010035 GO:0010119 GO:0010154 GO:0010182 GO:0010353 GO:0010449 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010896 GO:0016052 GO:0019216 GO:0019219 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031930 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0032880 GO:0033993 GO:0034284 GO:0034285 GO:0035266 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044042 GO:0044212 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048316 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048527 GO:0048528 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0050994 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071396 GO:0071495 GO:0071704 GO:0080090 GO:0090207 GO:0090696 GO:0097159 GO:0097305 GO:0097306 GO:0099402 GO:0140110 GO:1901363 GO:1901575 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

247

Amino Acids

27.76

Weight (kDa)

10.47

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 64 - 113 2.2e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40220
fragaria_vesca FvH4_7g24760
malus_domestica MD01G1155400.v1.1 MD07G1224400.v1.1
prunus_persica Prupe.2G253000_v2.0.a1
pyrus_communis pycom01g17270 pycom13g15020
rosa_chinensis RchiOBHm_Chr1g0370631
rosa_laevigata RLG00000027023
rosa_multiflora Rmu_sc0002021.1_g000011
rosa_roxburghii Rroxscaffold_4G00286390
rosa_rugosa Rorug01G0358000
rosa_samantha Rh1AG367500 Rh1BG330800 Rh1CG345000 Rh1DG362300
rosa_wichuraiana Rw1G032340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 192
AccB1I GGYRCC 1 cut(s) 192
AccBSI CCGCTC 1 cut(s) 468
AccII CGCG 5 cut(s) 21, 198, 250, 262, 394
AclWI GGATC 1 cut(s) 232
AcuI CTGAAG 1 cut(s) 54
AfaI GTAC 4 cut(s) 194, 601, 692, 730
AfiI CCNNNNNNNGG 4 cut(s) 25, 315, 417, 471
AflIII ACRYGT 1 cut(s) 635
AjiI CACGTC 1 cut(s) 638
AjnI CCWGG 1 cut(s) 341
AloI GAACNNNNNNTCC 2 cut(s) 466, 498
AluBI AGCT 3 cut(s) 32, 216, 352
AluI AGCT 3 cut(s) 32, 216, 352
AlwI GGATC 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 4 cut(s) 169, 318, 345, 426
ApaI GGGCCC 1 cut(s) 349
ApeKI GCWGC 5 cut(s) 29, 38, 299, 564, 623
AseI ATTAAT 1 cut(s) 530
Asp718I GGTACC 1 cut(s) 192
AspLEI GCGC 3 cut(s) 207, 258, 264
AspS9I GGNCC 7 cut(s) 170, 276, 318, 345, 346, 397, 462
AsuC2I CCSGG 2 cut(s) 317, 460
AsuHPI GGTGA 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 272
AvaII GGWCC 3 cut(s) 276, 397, 462
BaeGI GKGCMC 1 cut(s) 349
BanI GGYRCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 15
BbvI GCAGC 5 cut(s) 41, 50, 286, 576, 635
BccI CCATC 2 cut(s) 332, 613
BceAI ACGGC 1 cut(s) 636
BcgI CGANNNNNNTGC 4 cut(s) 253, 281, 287, 315
BciT130I CCWGG 1 cut(s) 343
BclI TGATCA 2 cut(s) 501, 672
BcnI CCSGG 2 cut(s) 317, 460
BfaI CTAG 1 cut(s) 665
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 8 cut(s) 30, 39, 199, 248, 300, 321, 565, 624
BlsI GCNGC 8 cut(s) 31, 40, 200, 249, 301, 322, 566, 625
Bme1390I CCNGG 3 cut(s) 317, 343, 460
Bme18I GGWCC 3 cut(s) 276, 397, 462
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 638
BmgT120I GGNCC 7 cut(s) 170, 276, 318, 345, 346, 397, 462
BmiI GGNNCC 7 cut(s) 194, 277, 340, 347, 373, 398, 464
BmrFI CCNGG 3 cut(s) 317, 343, 460
BpiI GAAGAC 1 cut(s) 15
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 1 cut(s) 344
BpuMI CCSGG 2 cut(s) 317, 460
BsaBI GATNNNNATC 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 196, 291, 342
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 2 cut(s) 389, 419
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 315, 417, 471
Bse8I GATNNNNATC 1 cut(s) 506
BseBI CCWGG 1 cut(s) 343
BseDI CCNNGG 3 cut(s) 196, 291, 342
BseGI GGATG 3 cut(s) 327, 654, 741
BseJI GATNNNNATC 1 cut(s) 506
BseLI CCNNNNNNNGG 4 cut(s) 25, 315, 417, 471
BseMII CTCAG 2 cut(s) 81, 225
BseRI GAGGAG 1 cut(s) 438
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 5 cut(s) 41, 50, 286, 576, 635
BseYI CCCAGC 2 cut(s) 216, 348
Bsh1236I CGCG 5 cut(s) 21, 198, 250, 262, 394
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 4 cut(s) 171, 320, 347, 428
BshNI GGYRCC 1 cut(s) 192
BsiEI CGRYCG 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 3 cut(s) 316, 369, 460
BsiWI CGTACG 1 cut(s) 728
BslFI GGGAC 4 cut(s) 289, 364, 410, 448
BslI CCNNNNNNNGG 4 cut(s) 25, 315, 417, 471
BsmFI GGGAC 4 cut(s) 289, 364, 410, 448
BsnI GGCC 4 cut(s) 171, 320, 347, 428
BsoBI CYCGRG 1 cut(s) 272
Bsp120I GGGCCC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 5 cut(s) 46, 237, 501, 507, 672
BspANI GGCC 4 cut(s) 171, 320, 347, 428
BspCNI CTCAG 2 cut(s) 82, 226
BspFNI CGCG 5 cut(s) 21, 198, 250, 262, 394
BspHI TCATGA 2 cut(s) 510, 669
BspLI GGNNCC 7 cut(s) 194, 277, 340, 347, 373, 398, 464
BspPI GGATC 1 cut(s) 232
BspT107I GGYRCC 1 cut(s) 192
BsrBI CCGCTC 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 196, 291, 342
BssMI GATC 5 cut(s) 46, 237, 501, 507, 672
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 338, 496
BstC8I GCNNGC 3 cut(s) 203, 209, 260
BstDEI CTNAG 2 cut(s) 90, 234
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 3 cut(s) 327, 654, 741
BstFNI CGCG 5 cut(s) 21, 198, 250, 262, 394
BstHHI GCGC 3 cut(s) 207, 258, 264
BstKTI GATC 5 cut(s) 49, 240, 504, 510, 675
BstMBI GATC 5 cut(s) 46, 237, 501, 507, 672
BstMCI CGRYCG 1 cut(s) 316
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 3 cut(s) 315, 341, 458
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 5 cut(s) 21, 198, 250, 262, 394
BstV1I GCAGC 5 cut(s) 41, 50, 286, 576, 635
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
BsuRI GGCC 4 cut(s) 171, 320, 347, 428
BtgI CCRYGG 1 cut(s) 196
BtgZI GCGATG 1 cut(s) 591
BtrI CACGTC 1 cut(s) 638
BtsCI GGATG 3 cut(s) 327, 654, 741
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 2 cut(s) 87, 285
Cac8I GCNNGC 3 cut(s) 203, 209, 260
CciI TCATGA 2 cut(s) 510, 669
CfoI GCGC 3 cut(s) 207, 258, 264
Cfr13I GGNCC 7 cut(s) 170, 276, 318, 345, 346, 397, 462
Cfr42I CCGCGG 1 cut(s) 199
CseI GACGC 1 cut(s) 305
Csp6I GTAC 4 cut(s) 193, 600, 691, 729
CviAII CATG 3 cut(s) 471, 511, 670
CviQI GTAC 4 cut(s) 193, 600, 691, 729
DdeI CTNAG 2 cut(s) 90, 234
DpnI GATC 5 cut(s) 48, 239, 503, 509, 674
DpnII GATC 5 cut(s) 46, 237, 501, 507, 672
Eco24I GRGCYC 1 cut(s) 349
Eco47I GGWCC 3 cut(s) 276, 397, 462
Eco57I CTGAAG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 3 cut(s) 170, 276, 345
EcoRII CCWGG 1 cut(s) 341
EcoT38I GRGCYC 1 cut(s) 349
FaeI CATG 3 cut(s) 474, 514, 673
FaiI YATR 4 cut(s) 472, 492, 512, 671
FaqI GGGAC 4 cut(s) 289, 364, 410, 448
FatI CATG 3 cut(s) 470, 510, 669
FauI CCCGC 3 cut(s) 387, 418, 473
FbaI TGATCA 2 cut(s) 501, 672
Fnu4HI GCNGC 8 cut(s) 30, 39, 199, 248, 300, 321, 565, 624
FokI GGATG 2 cut(s) 314, 641
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 8 cut(s) 30, 39, 199, 248, 300, 321, 565, 624
FspBI CTAG 1 cut(s) 665
GlaI GCGC 3 cut(s) 206, 257, 263
GluI GCNGC 8 cut(s) 30, 39, 199, 248, 300, 321, 565, 624
GsaI CCCAGC 2 cut(s) 220, 352
GsuI CTGGAG 1 cut(s) 344
HaeIII GGCC 4 cut(s) 171, 320, 347, 428
HapII CCGG 3 cut(s) 316, 369, 460
HgaI GACGC 1 cut(s) 305
HhaI GCGC 3 cut(s) 207, 258, 264
Hin1II CATG 3 cut(s) 474, 514, 673
Hin6I GCGC 3 cut(s) 205, 256, 262
HinP1I GCGC 3 cut(s) 205, 256, 262
HincII GTYRAC 1 cut(s) 641
HindII GTYRAC 1 cut(s) 641
HinfI GANTC 2 cut(s) 23, 475
HpaII CCGG 3 cut(s) 316, 369, 460
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 641
Hpy188I TCNGA 1 cut(s) 618
Hpy188III TCNNGA 5 cut(s) 274, 505, 511, 670, 715
Hpy8I GTNNAC 1 cut(s) 641
Hpy99I CGWCG 2 cut(s) 420, 622
HpyAV CCTTC 2 cut(s) 65, 289
HpyCH4III ACNGT 2 cut(s) 338, 496
HpyCH4IV ACGT 1 cut(s) 637
HpyCH4V TGCA 3 cut(s) 156, 540, 582
HpyF3I CTNAG 2 cut(s) 90, 234
HpySE526I ACGT 1 cut(s) 637
Hsp92II CATG 3 cut(s) 474, 514, 673
HspAI GCGC 3 cut(s) 205, 256, 262
KpnI GGTACC 1 cut(s) 196
Ksp22I TGATCA 2 cut(s) 501, 672
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 5 cut(s) 46, 237, 501, 507, 672
LmnI GCTCC 1 cut(s) 213
Lsp1109I GCAGC 5 cut(s) 41, 50, 286, 576, 635
MaeI CTAG 1 cut(s) 665
MaeII ACGT 1 cut(s) 637
MaeIII GTNAC 1 cut(s) 631
MalI GATC 5 cut(s) 48, 239, 503, 509, 674
MbiI CCGCTC 1 cut(s) 468
MboI GATC 5 cut(s) 46, 237, 501, 507, 672
MboII GAAGA 2 cut(s) 17, 20
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 2 cut(s) 572, 586
MmeI TCCRAC 1 cut(s) 641
MnlI CCTC 9 cut(s) 85, 161, 286, 376, 385, 456, 459, 654, 726
MseI TTAA 2 cut(s) 354, 530
MspA1I CMGCKG 4 cut(s) 32, 198, 211, 626
MspI CCGG 3 cut(s) 316, 369, 460
MspR9I CCNGG 3 cut(s) 317, 343, 460
MvaI CCWGG 1 cut(s) 343
MvnI CGCG 5 cut(s) 21, 198, 250, 262, 394
NciI CCSGG 2 cut(s) 317, 460
NdeII GATC 5 cut(s) 46, 237, 501, 507, 672
NlaIII CATG 3 cut(s) 474, 514, 673
NlaIV GGNNCC 7 cut(s) 194, 277, 340, 347, 373, 398, 464
NmeAIII GCCGAG 1 cut(s) 316
NmuCI GTSAC 1 cut(s) 631
PagI TCATGA 2 cut(s) 510, 669
PcsI WCGNNNNNNNCGW 3 cut(s) 288, 614, 643
PfeI GAWTC 2 cut(s) 23, 475
Pfl23II CGTACG 1 cut(s) 728
PkrI GCNGC 8 cut(s) 31, 40, 200, 249, 301, 322, 566, 625
PpuMI RGGWCCY 1 cut(s) 276
PshBI ATTAAT 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 341
PspFI CCCAGC 2 cut(s) 216, 348
PspGI CCWGG 1 cut(s) 341
PspLI CGTACG 1 cut(s) 728
PspN4I GGNNCC 7 cut(s) 194, 277, 340, 347, 373, 398, 464
PspOMI GGGCCC 1 cut(s) 345
PspPI GGNCC 7 cut(s) 170, 276, 318, 345, 346, 397, 462
PspPPI RGGWCCY 1 cut(s) 276
PsuI RGATCY 1 cut(s) 237
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 4 cut(s) 194, 601, 692, 730
RsaNI GTAC 4 cut(s) 193, 600, 691, 729
SacII CCGCGG 1 cut(s) 199
SaqAI TTAA 2 cut(s) 354, 530
SatI GCNGC 8 cut(s) 30, 39, 199, 248, 300, 321, 565, 624
Sau3AI GATC 5 cut(s) 46, 237, 501, 507, 672
Sau96I GGNCC 7 cut(s) 170, 276, 318, 345, 346, 397, 462
ScrFI CCNGG 3 cut(s) 317, 343, 460
SduI GDGCHC 1 cut(s) 349
SetI ASST 8 cut(s) 34, 57, 194, 218, 281, 354, 640, 646
Sfr303I CCGCGG 1 cut(s) 199
SgrBI CCGCGG 1 cut(s) 199
SinI GGWCC 3 cut(s) 276, 397, 462
Sse9I AATT 2 cut(s) 572, 586
SspMI CTAG 1 cut(s) 665
StyD4I CCNGG 3 cut(s) 315, 341, 458
TaaI ACNGT 2 cut(s) 338, 496
TaiI ACGT 1 cut(s) 640
TasI AATT 2 cut(s) 572, 586
TauI GCSGC 3 cut(s) 201, 250, 323
TfiI GAWTC 2 cut(s) 23, 475
Tru1I TTAA 2 cut(s) 354, 530
Tru9I TTAA 2 cut(s) 354, 530
TscAI CASTG 2 cut(s) 94, 292
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 5 cut(s) 29, 38, 299, 564, 623
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 580
TspGWI ACGGA 1 cut(s) 230
TspRI CASTG 2 cut(s) 94, 292
VpaK11BI GGWCC 3 cut(s) 276, 397, 462
VspI ATTAAT 1 cut(s) 530
XspI CTAG 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.