pycom01g20900

Protein phosphatase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008626
Physical Location & Seq
Reverse (-)
19190301 .. 19191735
1435 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 582 bp
ATGAAATTCTTAGAAATTGGCAGAGGCCGAGTTAGATGGGACGAGGCTAATATTGGAGAAATTGAAGCAAATAAACCTGTAAGGCAGAAAATTACTGAGCCGAAGACGCCATACCACCCCATGATGACCGATGATGAGTATGGATCTCTGTCTCCTATGCGGGGGGGTACCTTCGATGATTGTGTTGGTGATGCAGATCATGCAGAAGCAATACGAACTGCTTTGAGTGATGTGGCATCATCAAGTGGAAAGAGCACCCAACGGTCTACTGGCTGGACATCATCCGAGGATGAGGCTGATGCTATGGAACAAGATGATGAAGATTCTGAGAAAAGCAAGAGTTTTAGGGAGCACAGAAGAGCCCATTATGATGAATTCCAGAAAGTAAAAGAACTCAGGCGGAAAGCTTCTTTGCTCGATGATGAAGAGGAGGACGAGGACGAGAACCTTGACACAGATAAGGAGAAGGAGAAGAAATCTGGTTCCTCTTCGTTAACTGCCGGTGTCAAAGAAATAGATATCGACAAAGATGGTACCTCATCAACCAAAAGCTCTTCCGGACCTCCGGCGAATGGCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IPP-2

Protein Analysis

194

Amino Acids

21.4

Weight (kDa)

4.67

Isoelectric Point (pI)

52.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPP-2 PF04979 11 - 144 3.5e-24 Protein phosphatase inhibitor 2 (IPP-2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 167, 533
AccB1I GGYRCC 2 cut(s) 167, 533
AccI GTMKAC 1 cut(s) 266
AccIII TCCGGA 1 cut(s) 557
AciI CCGC 2 cut(s) 160, 400
AclWI GGATC 1 cut(s) 151
AcsI RAATTY 2 cut(s) 5, 374
AcyI GRCGYC 1 cut(s) 107
AfaI GTAC 2 cut(s) 169, 535
AfiI CCNNNNNNNGG 2 cut(s) 161, 572
AgsI TTSAA 1 cut(s) 65
AluBI AGCT 2 cut(s) 407, 552
AluI AGCT 2 cut(s) 407, 552
Alw21I GWGCWC 2 cut(s) 257, 354
Alw26I GTCTC 1 cut(s) 156
AlwI GGATC 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 557
AoxI GGCC 1 cut(s) 25
ApoI RAATTY 2 cut(s) 5, 374
Asp718I GGTACC 2 cut(s) 167, 533
AspLEI GCGC 1 cut(s) 578
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 1 cut(s) 200
AvaII GGWCC 1 cut(s) 560
BanI GGYRCC 2 cut(s) 167, 533
BanII GRGCYC 1 cut(s) 364
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
BbsI GAAGAC 1 cut(s) 110
Bbv12I GWGCWC 2 cut(s) 257, 354
BccI CCATC 2 cut(s) 30, 524
BcoDI GTCTC 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 579
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 3 cut(s) 169, 484, 535
BmsI GCATC 3 cut(s) 181, 245, 289
BpiI GAAGAC 1 cut(s) 110
BsaBI GATNNNNATC 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 107
BsaJI CCNNGG 1 cut(s) 285
BsaWI WCCGGW 1 cut(s) 557
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 572
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 1 cut(s) 274
Bse8I GATNNNNATC 1 cut(s) 195
BseAI TCCGGA 1 cut(s) 557
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 2 cut(s) 281, 295
BseJI GATNNNNATC 1 cut(s) 195
BseLI CCNNNNNNNGG 2 cut(s) 161, 572
BseMII CTCAG 3 cut(s) 87, 318, 409
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 443
BshFI GGCC 1 cut(s) 27
BshNI GGYRCC 2 cut(s) 167, 533
BsiHKAI GWGCWC 2 cut(s) 257, 354
BsiSI CCGG 3 cut(s) 501, 558, 566
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 161, 572
BsmAI GTCTC 1 cut(s) 156
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 1 cut(s) 27
Bsp1286I GDGCHC 3 cut(s) 257, 354, 364
Bsp13I TCCGGA 1 cut(s) 557
Bsp143I GATC 2 cut(s) 143, 196
BspACI CCGC 2 cut(s) 160, 400
BspANI GGCC 1 cut(s) 27
BspCNI CTCAG 3 cut(s) 88, 319, 408
BspEI TCCGGA 1 cut(s) 557
BspLI GGNNCC 3 cut(s) 169, 484, 535
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 2 cut(s) 352, 559
BspT107I GGYRCC 2 cut(s) 167, 533
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 1 cut(s) 274
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 2 cut(s) 143, 196
BssNI GRCGYC 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 264
Bst6I CTCTTC 4 cut(s) 352, 420, 493, 559
BstACI GRCGYC 1 cut(s) 107
BstAPI GCANNNNNTGC 1 cut(s) 200
BstDEI CTNAG 5 cut(s) 10, 96, 327, 395, 579
BstF5I GGATG 2 cut(s) 281, 295
BstH2I RGCGCY 1 cut(s) 579
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 2 cut(s) 146, 199
BstMAI GTCTC 1 cut(s) 156
BstMBI GATC 2 cut(s) 143, 196
BstMWI GCNNNNNNNGC 3 cut(s) 106, 200, 575
BstV2I GAAGAC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 1 cut(s) 27
BtsCI GGATG 2 cut(s) 281, 295
CfoI GCGC 1 cut(s) 578
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 560
CseI GACGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 168, 534
CviAII CATG 2 cut(s) 121, 200
CviJI RGCY 8 cut(s) 27, 47, 100, 273, 296, 362, 407, 552
CviKI_1 RGCY 8 cut(s) 27, 47, 100, 273, 296, 362, 407, 552
CviQI GTAC 2 cut(s) 168, 534
DdeI CTNAG 5 cut(s) 10, 96, 327, 395, 579
DpnI GATC 2 cut(s) 145, 198
DpnII GATC 2 cut(s) 143, 196
Eam1104I CTCTTC 4 cut(s) 352, 420, 493, 559
EarI CTCTTC 4 cut(s) 352, 420, 493, 559
EciI GGCGGA 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 364
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 374
EcoRV GATATC 1 cut(s) 520
EcoT38I GRGCYC 1 cut(s) 364
FaeI CATG 2 cut(s) 124, 203
FaiI YATR 7 cut(s) 112, 122, 141, 158, 201, 305, 369
FaqI GGGAC 1 cut(s) 53
FatI CATG 2 cut(s) 120, 199
FauI CCCGC 1 cut(s) 153
FblI GTMKAC 1 cut(s) 266
FokI GGATG 2 cut(s) 268, 302
FriOI GRGCYC 1 cut(s) 364
GlaI GCGC 1 cut(s) 577
HaeII RGCGCY 1 cut(s) 579
HaeIII GGCC 1 cut(s) 27
HapII CCGG 3 cut(s) 501, 558, 566
HgaI GACGC 1 cut(s) 115
HhaI GCGC 1 cut(s) 578
Hin1I GRCGYC 1 cut(s) 107
Hin1II CATG 2 cut(s) 124, 203
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 1 cut(s) 323
HpaI GTTAAC 1 cut(s) 495
HpaII CCGG 3 cut(s) 501, 558, 566
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 2 cut(s) 267, 495
Hpy188I TCNGA 2 cut(s) 286, 328
Hpy188III TCNNGA 2 cut(s) 379, 558
Hpy8I GTNNAC 2 cut(s) 267, 495
HpyAV CCTTC 2 cut(s) 181, 460
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4V TGCA 2 cut(s) 194, 203
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 200, 575
HpyF3I CTNAG 5 cut(s) 10, 96, 327, 395, 579
Hsp92I GRCGYC 1 cut(s) 107
Hsp92II CATG 2 cut(s) 124, 203
HspAI GCGC 1 cut(s) 576
Kpn2I TCCGGA 1 cut(s) 557
KpnI GGTACC 2 cut(s) 171, 537
KspAI GTTAAC 1 cut(s) 495
Kzo9I GATC 2 cut(s) 143, 196
LguI GCTCTTC 2 cut(s) 352, 559
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 8 cut(s) 90, 255, 259, 382, 392, 465, 514, 571
LweI GCATC 3 cut(s) 181, 245, 289
MalI GATC 2 cut(s) 145, 198
MboI GATC 2 cut(s) 143, 196
MboII GAAGA 7 cut(s) 115, 332, 369, 437, 480, 484, 546
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 3 cut(s) 257, 354, 364
MluCI AATT 5 cut(s) 5, 15, 60, 90, 374
MroI TCCGGA 1 cut(s) 557
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 3 cut(s) 501, 558, 566
MwoI GCNNNNNNNGC 3 cut(s) 106, 200, 575
NdeII GATC 2 cut(s) 143, 196
NlaIII CATG 2 cut(s) 124, 203
NlaIV GGNNCC 3 cut(s) 169, 484, 535
NmeAIII GCCGAG 1 cut(s) 53
PciSI GCTCTTC 2 cut(s) 352, 559
PfeI GAWTC 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 169, 484, 535
PspPI GGNCC 1 cut(s) 560
PsuI RGATCY 1 cut(s) 143
RsaI GTAC 2 cut(s) 169, 535
RsaNI GTAC 2 cut(s) 168, 534
RseI CAYNNNNRTG 1 cut(s) 369
SapI GCTCTTC 2 cut(s) 352, 559
SaqAI TTAA 1 cut(s) 494
Sau3AI GATC 2 cut(s) 143, 196
Sau96I GGNCC 1 cut(s) 560
SduI GDGCHC 3 cut(s) 257, 354, 364
SetI ASST 7 cut(s) 79, 173, 409, 450, 539, 554, 565
SfaNI GCATC 3 cut(s) 181, 245, 289
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 5 cut(s) 5, 15, 60, 90, 374
SsiI CCGC 2 cut(s) 160, 400
SspI AATATT 1 cut(s) 52
TaaI ACNGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 174, 417, 522
TaqII GACCGA 1 cut(s) 143
TasI AATT 5 cut(s) 5, 15, 60, 90, 374
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TspDTI ATGAA 4 cut(s) 17, 333, 387, 438
VpaK11BI GGWCC 1 cut(s) 560
XapI RAATTY 2 cut(s) 5, 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.