pycom01g21450

At3g49055-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0005360
Physical Location & Seq
Reverse (-)
19476118 .. 19477207
1090 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21450.1

Sequence Viewer

Length: 990 bp
ATGGAAAACCCTACAGAAACACTCCCAGAAACCCAGGTGAACGGCCCAGATCCGCCGAACAACCCAGAAACCTCCGAATCCGGTGATGGTCTTCTCGCCGAGCTCGAGTCCCTCCGCCAGTCGCACCAGGCCCTGCTGTCGAAGTCGGCGGCAATGGAGGAAGACTCTGATCTCATCCGGCAGCAGAGAGACGATGCCCTGGCTCGCAACGCCGAGCTAACCAAAGTCGTCGGAAAAATTTCCGGCGAGAGAGACTCGCTTCGCGACGAAATTCAGAAGCTCGAAGGTTGTCTGAGAGAAAAGGAAGATGGGTTTGACTCGAAATTGGAGGAGGAGCTGAAGTTGAAGGAGGTGATTGAAAACGAGGTGCGAGTTTGTCGGGAGACGATTGAAAGATTGGAGCTTGAGAGAAAAGAGAGAGATGGGTTTTTGCTGAAGTGCTTGGATTCGCTCCGATCGATCAAGGAGGGCATAATCGGAATAATCGACTGCGTCGGCGACGAGAAGGTAGGGAATGGGACTGAAGAAGCTGAGGGGACCGGTGCGGAATCGGGATCCGACCCAGAATGGAGGGCGGTTTATGAGGAGATTGCGGCGGTTGCCCGGCTGACGGCTTCGGCGGAAGCGCAGGTGAGTGAGTATAAAGAGCTGAGGAAGAAGGAGAAGAAGGAGTTGGAGAATAGCGTGGTGAGTCTGACGGAGGAGAACAGAGATATCAGCAGCCTGCTTAGGGTTGCTCTGGCGGAGAAGGAGGCGGTGGTGAAGAGGTTGAAAGGAAGCGAGCAGAAGCGGGTGCCTATTTTGCAGAGAGTTGGGTTTGGGTTTATAATGGGAAGTGGGGGCAATGAGCAGTCGCCGGAGAATTCCGGGGATAAGCTGGACGGTGCAGGGTCTACTGCAGGAAGTAAATCGGATGGCAGTGACTGTGAAGAGGGGGTCATCAGTCTGGCCTCGACTGTAGAGAAGATCGATGAAGAACCTGCGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

35.95

Weight (kDa)

4.56

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 827
AarI CACCTGC 1 cut(s) 619
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 793
AccI GTMKAC 1 cut(s) 893
AccII CGCG 1 cut(s) 264
AclWI GGATC 3 cut(s) 44, 549, 562
AcsI RAATTY 3 cut(s) 237, 270, 862
AcuI CTGAAG 3 cut(s) 359, 455, 543
AfiI CCNNNNNNNGG 2 cut(s) 610, 730
AgeI ACCGGT 1 cut(s) 539
AgsI TTSAA 4 cut(s) 346, 359, 392, 772
AjnI CCWGG 3 cut(s) 33, 126, 198
AjuI GAANNNNNNNTTGG 2 cut(s) 656, 688
AluBI AGCT 8 cut(s) 103, 217, 280, 337, 403, 530, 649, 877
AluI AGCT 8 cut(s) 103, 217, 280, 337, 403, 530, 649, 877
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 3 cut(s) 183, 246, 377
AlwI GGATC 3 cut(s) 44, 549, 562
AlwNI CAGNNNCTG 2 cut(s) 133, 924
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 3 cut(s) 43, 129, 948
ApeKI GCWGC 2 cut(s) 181, 720
ApoI RAATTY 3 cut(s) 237, 270, 862
AsiGI ACCGGT 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 628
AspS9I GGNCC 3 cut(s) 44, 130, 537
AsuC2I CCSGG 2 cut(s) 604, 868
AsuHPI GGTGA 6 cut(s) 49, 95, 364, 643, 700, 772
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 537
BamHI GGATCC 1 cut(s) 554
BanI GGYRCC 1 cut(s) 793
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 2 cut(s) 83, 168
Bbv12I GWGCWC 1 cut(s) 105
BbvCI CCTCAGC 2 cut(s) 531, 650
BbvI GCAGC 2 cut(s) 193, 732
BccI CCATC 4 cut(s) 80, 302, 416, 908
BceAI ACGGC 2 cut(s) 58, 627
BciT130I CCWGG 3 cut(s) 35, 128, 200
BcnI CCSGG 2 cut(s) 604, 868
BcoDI GTCTC 3 cut(s) 183, 246, 377
BfmI CTRYAG 3 cut(s) 12, 897, 957
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 5 cut(s) 150, 182, 594, 721, 984
BlsI GCNGC 5 cut(s) 151, 183, 595, 722, 985
Bme1390I CCNGG 5 cut(s) 35, 128, 200, 604, 868
Bme18I GGWCC 1 cut(s) 537
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 44, 130, 537
BmiI GGNNCC 3 cut(s) 538, 556, 795
BmrFI CCNGG 5 cut(s) 35, 128, 200, 604, 868
BmsI GCATC 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 83, 168
BplI GAGNNNNNCTC 4 cut(s) 149, 181, 239, 271
Bpu10I CCTNAGC 3 cut(s) 531, 650, 728
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 2 cut(s) 604, 868
Bsa29I ATCGAT 2 cut(s) 458, 969
BsaJI CCNNGG 3 cut(s) 33, 198, 867
BsaWI WCCGGW 2 cut(s) 80, 539
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 610, 730
Bse118I RCCGGY 1 cut(s) 539
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 2 cut(s) 159, 850
BseBI CCWGG 3 cut(s) 35, 128, 200
BseCI ATCGAT 2 cut(s) 458, 969
BseDI CCNNGG 3 cut(s) 33, 198, 867
BseGI GGATG 2 cut(s) 174, 919
BseLI CCNNNNNNNGG 2 cut(s) 610, 730
BseMI GCAATG 2 cut(s) 159, 850
BseMII CTCAG 3 cut(s) 284, 522, 641
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 4 cut(s) 344, 347, 599, 716
BseXI GCAGC 2 cut(s) 193, 732
BsgI GTGCAG 1 cut(s) 906
Bsh1236I CGCG 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 3 cut(s) 45, 131, 950
BshNI GGYRCC 1 cut(s) 793
BshTI ACCGGT 1 cut(s) 539
BshVI ATCGAT 2 cut(s) 458, 969
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 7 cut(s) 81, 178, 243, 540, 604, 857, 867
BslFI GGGAC 3 cut(s) 94, 532, 550
BslI CCNNNNNNNGG 2 cut(s) 610, 730
BsmAI GTCTC 3 cut(s) 183, 246, 377
BsmBI CGTCTC 2 cut(s) 183, 377
BsmFI GGGAC 3 cut(s) 94, 532, 550
BsnI GGCC 3 cut(s) 45, 131, 950
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 6 cut(s) 49, 169, 455, 459, 554, 966
Bsp68I TCGCGA 1 cut(s) 264
BspANI GGCC 3 cut(s) 45, 131, 950
BspCNI CTCAG 3 cut(s) 285, 523, 642
BspDI ATCGAT 2 cut(s) 458, 969
BspFNI CGCG 1 cut(s) 264
BspLI GGNNCC 3 cut(s) 538, 556, 795
BspMAI CTGCAG 1 cut(s) 901
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 3 cut(s) 44, 549, 562
BspT107I GGYRCC 1 cut(s) 793
BsrDI GCAATG 2 cut(s) 159, 850
BsrFI RCCGGY 1 cut(s) 539
BsrI ACTGG 1 cut(s) 118
BssAI RCCGGY 1 cut(s) 539
BssECI CCNNGG 3 cut(s) 33, 198, 867
BssMI GATC 6 cut(s) 49, 169, 455, 459, 554, 966
Bst2UI CCWGG 3 cut(s) 35, 128, 200
Bst4CI ACNGT 3 cut(s) 884, 926, 958
Bst6I CTCTTC 2 cut(s) 758, 924
BstC8I GCNNGC 3 cut(s) 205, 725, 782
BstDEI CTNAG 4 cut(s) 293, 531, 650, 728
BstF5I GGATG 2 cut(s) 174, 919
BstFNI CGCG 1 cut(s) 264
BstHHI GCGC 1 cut(s) 628
BstKTI GATC 6 cut(s) 52, 172, 458, 462, 557, 969
BstMAI GTCTC 3 cut(s) 183, 246, 377
BstMBI GATC 6 cut(s) 49, 169, 455, 459, 554, 966
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 3 cut(s) 209, 599, 802
BstNI CCWGG 3 cut(s) 35, 128, 200
BstSCI CCNGG 5 cut(s) 33, 126, 198, 602, 866
BstSFI CTRYAG 3 cut(s) 12, 897, 957
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 2 cut(s) 193, 732
BstV2I GAAGAC 2 cut(s) 83, 168
BstX2I RGATCY 2 cut(s) 49, 554
BstYI RGATCY 2 cut(s) 49, 554
Bsu15I ATCGAT 2 cut(s) 458, 969
BsuRI GGCC 3 cut(s) 45, 131, 950
BsuTUI ATCGAT 2 cut(s) 458, 969
BtsCI GGATG 2 cut(s) 174, 919
BtsI GCAGTG 1 cut(s) 925
BtsIMutI CAGTG 1 cut(s) 925
BtuMI TCGCGA 1 cut(s) 264
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 3 cut(s) 205, 725, 782
CaiI CAGNNNCTG 2 cut(s) 133, 924
CfoI GCGC 1 cut(s) 628
Cfr10I RCCGGY 1 cut(s) 539
Cfr13I GGNCC 3 cut(s) 44, 130, 537
ClaI ATCGAT 2 cut(s) 458, 969
CseI GACGC 1 cut(s) 481
CspAI ACCGGT 1 cut(s) 539
DdeI CTNAG 4 cut(s) 293, 531, 650, 728
DpnI GATC 6 cut(s) 51, 171, 457, 461, 556, 968
DpnII GATC 6 cut(s) 49, 169, 455, 459, 554, 966
Eam1104I CTCTTC 2 cut(s) 758, 924
EarI CTCTTC 2 cut(s) 758, 924
EciI GGCGGA 4 cut(s) 42, 104, 635, 758
Ecl136II GAGCTC 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 537
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 3 cut(s) 359, 455, 543
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 130
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 3 cut(s) 33, 126, 198
EcoRV GATATC 1 cut(s) 715
EcoT38I GRGCYC 1 cut(s) 105
Esp3I CGTCTC 2 cut(s) 183, 377
FaiI YATR 4 cut(s) 473, 582, 642, 827
FaqI GGGAC 3 cut(s) 94, 532, 550
FauI CCCGC 1 cut(s) 783
FblI GTMKAC 1 cut(s) 893
Fnu4HI GCNGC 5 cut(s) 150, 182, 594, 721, 984
FokI GGATG 2 cut(s) 161, 926
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 5 cut(s) 150, 182, 594, 721, 984
GlaI GCGC 1 cut(s) 627
GluI GCNGC 5 cut(s) 150, 182, 594, 721, 984
HaeIII GGCC 3 cut(s) 45, 131, 950
HapII CCGG 7 cut(s) 81, 178, 243, 540, 604, 857, 867
HgaI GACGC 1 cut(s) 481
HhaI GCGC 1 cut(s) 628
Hin6I GCGC 1 cut(s) 626
HinP1I GCGC 1 cut(s) 626
HinfI GANTC 8 cut(s) 77, 107, 164, 254, 317, 446, 548, 691
HpaII CCGG 7 cut(s) 81, 178, 243, 540, 604, 857, 867
HphI GGTGA 6 cut(s) 49, 95, 364, 643, 700, 772
Hpy166II GTNNAC 2 cut(s) 40, 894
Hpy188III TCNNGA 3 cut(s) 263, 380, 552
Hpy8I GTNNAC 2 cut(s) 40, 894
Hpy99I CGWCG 4 cut(s) 233, 269, 497, 503
HpyAV CCTTC 6 cut(s) 278, 340, 499, 652, 661, 742
HpyCH4III ACNGT 3 cut(s) 884, 926, 958
HpyCH4V TGCA 3 cut(s) 805, 887, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 599, 802
HpyF3I CTNAG 4 cut(s) 293, 531, 650, 728
HspAI GCGC 1 cut(s) 626
Kzo9I GATC 6 cut(s) 49, 169, 455, 459, 554, 966
LmnI GCTCC 3 cut(s) 334, 400, 456
Lsp1109I GCAGC 2 cut(s) 193, 732
LweI GCATC 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 920
MalI GATC 6 cut(s) 51, 171, 457, 461, 556, 968
MboI GATC 6 cut(s) 49, 169, 455, 459, 554, 966
MflI RGATCY 2 cut(s) 49, 554
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 4 cut(s) 237, 270, 323, 862
MlyI GAGTC 5 cut(s) 116, 158, 248, 311, 700
MmeI TCCRAC 3 cut(s) 211, 582, 654
MroXI GAANNNNTTC 1 cut(s) 238
MspI CCGG 7 cut(s) 81, 178, 243, 540, 604, 857, 867
MspR9I CCNGG 5 cut(s) 35, 128, 200, 604, 868
MvaI CCWGG 3 cut(s) 35, 128, 200
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 3 cut(s) 209, 599, 802
NciI CCSGG 2 cut(s) 604, 868
NdeII GATC 6 cut(s) 49, 169, 455, 459, 554, 966
NlaIV GGNNCC 3 cut(s) 538, 556, 795
NmeAIII GCCGAG 2 cut(s) 124, 238
NmuCI GTSAC 1 cut(s) 920
NruI TCGCGA 1 cut(s) 264
PaeR7I CTCGAG 1 cut(s) 104
PaqCI CACCTGC 1 cut(s) 619
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 455, 483
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 77, 446, 548
PflFI GACNNNGTC 1 cut(s) 491
PinAI ACCGGT 1 cut(s) 539
PkrI GCNGC 5 cut(s) 151, 183, 595, 722, 985
Ple19I CGATCG 1 cut(s) 458
PleI GAGTC 5 cut(s) 115, 158, 248, 311, 699
PpsI GAGTC 5 cut(s) 115, 158, 248, 311, 699
PsiI TTATAA 1 cut(s) 827
Psp124BI GAGCTC 1 cut(s) 105
Psp6I CCWGG 3 cut(s) 33, 126, 198
PspGI CCWGG 3 cut(s) 33, 126, 198
PspN4I GGNNCC 3 cut(s) 538, 556, 795
PspPI GGNCC 3 cut(s) 44, 130, 537
PspXI VCTCGAGB 1 cut(s) 104
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 2 cut(s) 133, 924
PsuI RGATCY 2 cut(s) 49, 554
PsyI GACNNNGTC 1 cut(s) 491
PvuI CGATCG 1 cut(s) 458
RruI TCGCGA 1 cut(s) 264
SacI GAGCTC 1 cut(s) 105
SatI GCNGC 5 cut(s) 150, 182, 594, 721, 984
Sau3AI GATC 6 cut(s) 49, 169, 455, 459, 554, 966
Sau96I GGNCC 3 cut(s) 44, 130, 537
SchI GAGTC 5 cut(s) 116, 158, 248, 311, 700
ScrFI CCNGG 5 cut(s) 35, 128, 200, 604, 868
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 1 cut(s) 184
SfcI CTRYAG 3 cut(s) 12, 897, 957
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 537
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 104, 404
SmoI CTYRAG 2 cut(s) 104, 404
Sse9I AATT 4 cut(s) 237, 270, 323, 862
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 5 cut(s) 33, 126, 198, 602, 866
TaaI ACNGT 3 cut(s) 884, 926, 958
TaqI TCGA 8 cut(s) 105, 140, 282, 320, 458, 486, 953, 969
TasI AATT 4 cut(s) 237, 270, 323, 862
TauI GCSGC 3 cut(s) 152, 596, 986
TfiI GAWTC 3 cut(s) 77, 446, 548
TscAI CASTG 1 cut(s) 925
TseFI GTSAC 1 cut(s) 920
TseI GCWGC 2 cut(s) 181, 720
Tsp45I GTSAC 1 cut(s) 920
TspDTI ATGAA 1 cut(s) 987
TspGWI ACGGA 1 cut(s) 713
TspRI CASTG 1 cut(s) 925
Tth111I GACNNNGTC 1 cut(s) 491
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 3 cut(s) 237, 270, 862
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 893
XmnI GAANNNNTTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.