Rroxscaffold_4G00281050

At3g49055-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005360
Physical Location & Seq
Forward (+)
3444818 .. 3447443
2626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281050.1

Sequence Viewer

Length: 1590 bp
ATGGAAACCGAAATCAACGGCCCAGATTCCACAGTAGCCAATCATGATCTCCTCCGCGCCGAGCTCGACTCCCTCCGCCGGTCTCACCGGAGCATCCTACAGAAGACGGCGGCGATGGAGGAAGACTTCAACCGCGTCCAGAGCCAGCGAGACGAGGCGGCAGCTCGCAACGCCGAGTTGACCAGACTCGTCGGAGAAGTTTCTGGCGAGAGGGACGCTCTAAGGGAAGAGCTTCGCAAGGTCGAAGCTGTGGAGAGAGAGAAGGAAGACGAGTATGTGATGAAAATCGATGAGGAGGTGAGGAGGAAGAAGAGTCTGGAAAGTGAGGTTCGAGTTTGTAAGGAGAGAATTGAGAGGCTGGAGACTGAGAGGAGAGAAAGAGATGAGTTTTTGTTCAAGTGCTTGGATTCGCTCCGGCCGATCGAGAAGGAGTTGATCGGGATTATTGAAGGAGTCTGTGACGAAGAAGATGACAAACAAGTAGTTGAGATTGAGAAGGCGGCAACGAATGTGGGGGAAGGTGAAGCCGAATTGGACCAGCAATCGAGGGGAGTATGGGAGGAGATCAAGACTATCAAGAGGCTAGTGGAGGTAGCCGAAGAGAAAGTGGATGATTATAAAGAATTGAGGCATAACGAGAGGAGGCAATTGGGGAATAGTGTGGTGAGTTTGACAGAGGAGAATAGGGATATCAGCACATTGCTGAGGATTGCTTTGGTGGAGAAGGAGGCTGTGGAGAAGAGGTTGAAGGGGATCGGTGAGACGAAGAGGGTGGCCATTTTGCAGAGAGTTGGATTCAGTCCGTTCGGGTTTTTGATGGGAAGTGGAGGCAATGAGCAGTCATTGGAGGCTTCCGGGGCTAAATTGGATGGAGCTAAATTGGAAGGAATGGGGTCTGGTGCGAGTAACAAGTCGGATAGCAGTGAGTGTGAAGAGGAGGTTGTGAGTCTGGCATCTACTGTAGAGAAGATAATGAAGAACCTGCGCCTGGAAATCACTCAATTGAGAAGATCTTTGGACGACTCTAGGTCAGACACTGAGCGCCTACAGAGTCTCACAGGGAAACAAGCTCAACAAATTGCAGAAAACATGCTGTACATCAAGGAGTTAGAAGACAGAGAGAGGGTGTTGACTGAAAATGTTGAGGCACACATGATTGAAATGAAGGATGCTGAAGCAGAGGTTGCCAGATGGAGAGAAGCTTGTGAGTTGGAAGTTGAAGCTGGGAAAAATGAGATTGCAGAACGCGAAAAATTGATTGCCATTCTGAAGCAAGAATTGGAGAAAACAAGGGCTGCTTTAGACATATCAAATGGCAAATTAAAGCTGAAAGAAGAACTTGCAGCTAATGCCATGGCTGCTCATGCAGCAGCAGAGAAGTCCCTCCAGCTGGCTGACAGCAGGGCAGCAGGACTCCGTACCCGAATAGAGGAACTAACAAGACAGCTAGAAGAAGCAGATAGCAGAGAGCGCAATAGCCGAAGGGTGAGACATATATGTTGGCCATGGCGATCCTTCAAAGTGAACCCCGCTAACAATGTACGACGATTGCTACCAGAAATGCAGGCCTTGCTTAATTATCGTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

529

Amino Acids

60.36

Weight (kDa)

5.14

Isoelectric Point (pI)

60.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
Acc36I ACCTGC 1 cut(s) 990
AccII CGCG 3 cut(s) 57, 135, 1248
AciI CCGC 7 cut(s) 55, 76, 110, 133, 158, 500, 1530
AclWI GGATC 2 cut(s) 761, 1506
AcoI YGGCCR 3 cut(s) 416, 774, 1502
AcuI CTGAAG 2 cut(s) 1194, 1289
AfaI GTAC 3 cut(s) 1097, 1420, 1542
AfiI CCNNNNNNNGG 4 cut(s) 78, 988, 1390, 1429
AgsI TTSAA 7 cut(s) 130, 397, 449, 748, 1160, 1220, 1519
AhdI GACNNNNNGTC 1 cut(s) 1027
AjnI CCWGG 1 cut(s) 987
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
Alw21I GWGCWC 1 cut(s) 66
Alw26I GTCTC 6 cut(s) 87, 144, 356, 755, 1058, 1483
AlwI GGATC 2 cut(s) 761, 1506
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 5 cut(s) 19, 416, 774, 1502, 1566
ApeKI GCWGC 7 cut(s) 161, 1295, 1343, 1358, 1367, 1370, 1406
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 4 cut(s) 59, 987, 1044, 1473
AspS9I GGNCC 2 cut(s) 20, 535
AsuC2I CCSGG 1 cut(s) 856
AsuHPI GGTGA 6 cut(s) 77, 310, 533, 676, 770, 1498
AvaII GGWCC 1 cut(s) 535
BalI TGGCCA 2 cut(s) 776, 1504
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 4 cut(s) 110, 129, 273, 1119
Bbv12I GWGCWC 1 cut(s) 66
BbvCI CCTCAGC 1 cut(s) 704
BbvI GCAGC 7 cut(s) 173, 1282, 1345, 1355, 1379, 1382, 1418
BccI CCATC 4 cut(s) 109, 811, 863, 1185
BceAI ACGGC 2 cut(s) 34, 123
BciT130I CCWGG 1 cut(s) 989
BcnI CCSGG 1 cut(s) 856
BcoDI GTCTC 6 cut(s) 87, 144, 356, 755, 1058, 1483
BfaI CTAG 3 cut(s) 584, 1026, 1448
BfmI CTRYAG 3 cut(s) 98, 960, 1046
BfoI RGCGCY 1 cut(s) 1045
BfuAI ACCTGC 1 cut(s) 990
BglII AGATCT 1 cut(s) 1010
Bme1390I CCNGG 2 cut(s) 856, 989
Bme18I GGWCC 1 cut(s) 535
BmeRI GACNNNNNGTC 1 cut(s) 1027
BmgT120I GGNCC 2 cut(s) 20, 535
BmrFI CCNGG 2 cut(s) 856, 989
BmsI GCATC 3 cut(s) 102, 962, 1159
BpiI GAAGAC 4 cut(s) 110, 129, 273, 1119
BplI GAGNNNNNCTC 4 cut(s) 53, 85, 202, 234
BpmI CTGGAG 2 cut(s) 380, 1370
Bpu10I CCTNAGC 1 cut(s) 704
BpuMI CCSGG 1 cut(s) 856
Bsa29I ATCGAT 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 284
BsaI GGTCTC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 855, 1353, 1505
BsaWI WCCGGW 1 cut(s) 87
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 988, 1390, 1429
Bse118I RCCGGY 1 cut(s) 78
Bse3DI GCAATG 2 cut(s) 698, 838
Bse8I GATNNNNATC 1 cut(s) 284
BseBI CCWGG 1 cut(s) 989
BseCI ATCGAT 1 cut(s) 288
BseDI CCNNGG 3 cut(s) 855, 1353, 1505
BseGI GGATG 4 cut(s) 93, 616, 874, 1174
BseJI GATNNNNATC 1 cut(s) 284
BseLI CCNNNNNNNGG 4 cut(s) 78, 988, 1390, 1429
BseMI GCAATG 2 cut(s) 698, 838
BseMII CTCAG 3 cut(s) 357, 695, 1029
BseRI GAGGAG 8 cut(s) 41, 308, 316, 385, 575, 655, 692, 950
BseX3I CGGCCG 1 cut(s) 416
BseXI GCAGC 7 cut(s) 173, 1282, 1345, 1355, 1379, 1382, 1418
BseYI CCCAGC 1 cut(s) 1223
Bsh1236I CGCG 3 cut(s) 57, 135, 1248
Bsh1285I CGRYCG 2 cut(s) 419, 423
BshFI GGCC 5 cut(s) 21, 418, 776, 1504, 1568
BshVI ATCGAT 1 cut(s) 288
BsiEI CGRYCG 2 cut(s) 419, 423
BsiHKAI GWGCWC 1 cut(s) 66
BsiSI CCGG 4 cut(s) 79, 88, 415, 855
BslFI GGGAC 2 cut(s) 227, 1366
BslI CCNNNNNNNGG 4 cut(s) 78, 988, 1390, 1429
BsmAI GTCTC 6 cut(s) 87, 144, 356, 755, 1058, 1483
BsmBI CGTCTC 2 cut(s) 144, 755
BsmFI GGGAC 2 cut(s) 227, 1366
BsnI GGCC 5 cut(s) 21, 418, 776, 1504, 1568
Bso31I GGTCTC 1 cut(s) 87
Bsp1286I GDGCHC 1 cut(s) 66
Bsp1407I TGTACA 1 cut(s) 1095
Bsp143I GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
Bsp19I CCATGG 2 cut(s) 1353, 1505
BspACI CCGC 7 cut(s) 55, 76, 110, 133, 158, 500, 1530
BspANI GGCC 5 cut(s) 21, 418, 776, 1504, 1568
BspCNI CTCAG 3 cut(s) 358, 696, 1030
BspDI ATCGAT 1 cut(s) 288
BspFNI CGCG 3 cut(s) 57, 135, 1248
BspHI TCATGA 1 cut(s) 43
BspMI ACCTGC 1 cut(s) 990
BspPI GGATC 2 cut(s) 761, 1506
BspQI GCTCTTC 1 cut(s) 222
BspTNI GGTCTC 1 cut(s) 87
BsrDI GCAATG 2 cut(s) 698, 838
BsrFI RCCGGY 1 cut(s) 78
BsrGI TGTACA 1 cut(s) 1095
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 3 cut(s) 855, 1353, 1505
BssMI GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
BssT1I CCWWGG 2 cut(s) 1353, 1505
Bst2UI CCWGG 1 cut(s) 989
Bst4CI ACNGT 2 cut(s) 34, 961
Bst6I CTCTTC 6 cut(s) 222, 305, 594, 734, 761, 927
BstAPI GCANNNNNTGC 3 cut(s) 1184, 1349, 1570
BstAUI TGTACA 1 cut(s) 1095
BstC8I GCNNGC 4 cut(s) 146, 166, 1392, 1566
BstDEI CTNAG 4 cut(s) 221, 366, 704, 1038
BstDSI CCRYGG 2 cut(s) 1353, 1505
BstF5I GGATG 4 cut(s) 93, 616, 874, 1174
BstFNI CGCG 3 cut(s) 57, 135, 1248
BstH2I RGCGCY 1 cut(s) 1045
BstHHI GCGC 4 cut(s) 59, 987, 1044, 1473
BstKTI GATC 7 cut(s) 49, 423, 438, 567, 756, 1013, 1514
BstMAI GTCTC 6 cut(s) 87, 144, 356, 755, 1058, 1483
BstMBI GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
BstMCI CGRYCG 2 cut(s) 419, 423
BstNI CCWGG 1 cut(s) 989
BstNSI RCATGY 1 cut(s) 1093
BstSCI CCNGG 2 cut(s) 854, 987
BstSFI CTRYAG 3 cut(s) 98, 960, 1046
BstUI CGCG 3 cut(s) 57, 135, 1248
BstV1I GCAGC 7 cut(s) 173, 1282, 1345, 1355, 1379, 1382, 1418
BstV2I GAAGAC 4 cut(s) 110, 129, 273, 1119
BstX2I RGATCY 1 cut(s) 1010
BstYI RGATCY 1 cut(s) 1010
BstZI CGGCCG 1 cut(s) 416
Bsu15I ATCGAT 1 cut(s) 288
BsuRI GGCC 5 cut(s) 21, 418, 776, 1504, 1568
BsuTUI ATCGAT 1 cut(s) 288
BtgI CCRYGG 2 cut(s) 1353, 1505
BtgZI GCGATG 1 cut(s) 128
BtsCI GGATG 4 cut(s) 93, 616, 874, 1174
BtsI GCAGTG 1 cut(s) 928
BtsIMutI CAGTG 2 cut(s) 928, 1035
BveI ACCTGC 1 cut(s) 990
Cac8I GCNNGC 4 cut(s) 146, 166, 1392, 1566
CaiI CAGNNNCTG 1 cut(s) 1037
CciI TCATGA 1 cut(s) 43
CfoI GCGC 4 cut(s) 59, 987, 1044, 1473
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 2 cut(s) 20, 535
ClaI ATCGAT 1 cut(s) 288
CseI GACGC 2 cut(s) 124, 224
Csp6I GTAC 3 cut(s) 1096, 1419, 1541
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviAII CATG 6 cut(s) 44, 1090, 1153, 1354, 1364, 1506
CviQI GTAC 3 cut(s) 1096, 1419, 1541
DdeI CTNAG 4 cut(s) 221, 366, 704, 1038
DpnI GATC 7 cut(s) 48, 422, 437, 566, 755, 1012, 1513
DpnII GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
DriI GACNNNNNGTC 1 cut(s) 1027
EaeI YGGCCR 3 cut(s) 416, 774, 1502
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 6 cut(s) 222, 305, 594, 734, 761, 927
Eam1105I GACNNNNNGTC 1 cut(s) 1027
EarI CTCTTC 6 cut(s) 222, 305, 594, 734, 761, 927
EciI GGCGGA 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 64
EclXI CGGCCG 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 1353, 1505
Eco147I AGGCCT 1 cut(s) 1568
Eco24I GRGCYC 1 cut(s) 66
Eco31I GGTCTC 1 cut(s) 87
Eco32I GATATC 1 cut(s) 691
Eco47I GGWCC 1 cut(s) 535
Eco52I CGGCCG 1 cut(s) 416
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 2 cut(s) 1194, 1289
EcoICRI GAGCTC 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 987
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 2 cut(s) 1353, 1505
EcoT38I GRGCYC 1 cut(s) 66
ErhI CCWWGG 2 cut(s) 1353, 1505
Esp3I CGTCTC 2 cut(s) 144, 755
FaeI CATG 6 cut(s) 47, 1093, 1156, 1357, 1367, 1509
FalI AAGNNNNNCTT 4 cut(s) 1282, 1314, 1323, 1355
FaqI GGGAC 2 cut(s) 227, 1366
FatI CATG 6 cut(s) 43, 1089, 1152, 1353, 1363, 1505
FauI CCCGC 1 cut(s) 1537
FokI GGATG 4 cut(s) 80, 623, 881, 1181
FriOI GRGCYC 1 cut(s) 66
FspBI CTAG 3 cut(s) 584, 1026, 1448
GlaI GCGC 4 cut(s) 58, 986, 1043, 1472
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 2 cut(s) 380, 1370
HaeII RGCGCY 1 cut(s) 1045
HaeIII GGCC 5 cut(s) 21, 418, 776, 1504, 1568
HapII CCGG 4 cut(s) 79, 88, 415, 855
HgaI GACGC 2 cut(s) 124, 224
HhaI GCGC 4 cut(s) 59, 987, 1044, 1473
Hin1II CATG 6 cut(s) 47, 1093, 1156, 1357, 1367, 1509
Hin6I GCGC 4 cut(s) 57, 985, 1042, 1471
HinP1I GCGC 4 cut(s) 57, 985, 1042, 1471
HincII GTYRAC 2 cut(s) 180, 1131
HindII GTYRAC 2 cut(s) 180, 1131
HindIII AAGCTT 1 cut(s) 1200
HpaII CCGG 4 cut(s) 79, 88, 415, 855
HphI GGTGA 6 cut(s) 77, 310, 533, 676, 770, 1498
Hpy166II GTNNAC 3 cut(s) 180, 1131, 1525
Hpy188I TCNGA 4 cut(s) 194, 916, 1033, 1269
Hpy188III TCNNGA 7 cut(s) 44, 139, 317, 424, 439, 568, 577
Hpy8I GTNNAC 3 cut(s) 180, 1131, 1525
Hpy99I CGWCG 2 cut(s) 194, 1548
HpyCH4III ACNGT 2 cut(s) 34, 961
HpyCH4V TGCA 6 cut(s) 784, 1082, 1241, 1343, 1367, 1564
HpyF3I CTNAG 4 cut(s) 221, 366, 704, 1038
Hsp92II CATG 6 cut(s) 47, 1093, 1156, 1357, 1367, 1509
HspAI GCGC 4 cut(s) 57, 985, 1042, 1471
Kzo9I GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 3 cut(s) 90, 417, 872
Lsp1109I GCAGC 7 cut(s) 173, 1282, 1345, 1355, 1379, 1382, 1418
LweI GCATC 3 cut(s) 102, 962, 1159
MaeI CTAG 3 cut(s) 584, 1026, 1448
MaeIII GTNAC 2 cut(s) 458, 905
MalI GATC 7 cut(s) 48, 422, 437, 566, 755, 1012, 1513
MboI GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
MfeI CAATTG 2 cut(s) 647, 1001
MflI RGATCY 1 cut(s) 1010
MhlI GDGCHC 1 cut(s) 66
MlsI TGGCCA 2 cut(s) 776, 1504
MluNI TGGCCA 2 cut(s) 776, 1504
MlyI GAGTC 8 cut(s) 62, 180, 322, 462, 955, 1016, 1060, 1407
MmeI TCCRAC 4 cut(s) 172, 772, 894, 1191
Mox20I TGGCCA 2 cut(s) 776, 1504
MroXI GAANNNNTTC 1 cut(s) 231
MscI TGGCCA 2 cut(s) 776, 1504
MseI TTAA 2 cut(s) 1322, 1575
Msp20I TGGCCA 2 cut(s) 776, 1504
MspA1I CMGCKG 1 cut(s) 1390
MspI CCGG 4 cut(s) 79, 88, 415, 855
MspR9I CCNGG 2 cut(s) 856, 989
MunI CAATTG 2 cut(s) 647, 1001
MvaI CCWGG 1 cut(s) 989
MvnI CGCG 3 cut(s) 57, 135, 1248
NciI CCSGG 1 cut(s) 856
NcoI CCATGG 2 cut(s) 1353, 1505
NdeII GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
NlaIII CATG 6 cut(s) 47, 1093, 1156, 1357, 1367, 1509
NmeAIII GCCGAG 2 cut(s) 85, 199
NmuCI GTSAC 1 cut(s) 458
NspI RCATGY 1 cut(s) 1093
PagI TCATGA 1 cut(s) 43
PceI AGGCCT 1 cut(s) 1568
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 3 cut(s) 26, 407, 795
Ple19I CGATCG 1 cut(s) 423
PleI GAGTC 8 cut(s) 62, 180, 321, 461, 954, 1016, 1059, 1407
PpsI GAGTC 8 cut(s) 62, 180, 321, 461, 954, 1016, 1059, 1407
PsiI TTATAA 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 987
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 1 cut(s) 987
PspPI GGNCC 2 cut(s) 20, 535
PstNI CAGNNNCTG 1 cut(s) 1037
PsuI RGATCY 1 cut(s) 1010
PvuI CGATCG 1 cut(s) 423
PvuII CAGCTG 1 cut(s) 1390
RsaI GTAC 3 cut(s) 1097, 1420, 1542
RsaNI GTAC 3 cut(s) 1096, 1419, 1541
SacI GAGCTC 1 cut(s) 66
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 2 cut(s) 1322, 1575
Sau3AI GATC 7 cut(s) 46, 420, 435, 564, 753, 1010, 1511
Sau96I GGNCC 2 cut(s) 20, 535
SchI GAGTC 8 cut(s) 62, 180, 322, 462, 955, 1016, 1060, 1407
ScrFI CCNGG 2 cut(s) 856, 989
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 102, 962, 1159
SfcI CTRYAG 3 cut(s) 98, 960, 1046
SinI GGWCC 1 cut(s) 535
SseBI AGGCCT 1 cut(s) 1568
SsiI CCGC 7 cut(s) 55, 76, 110, 133, 158, 500, 1530
SspMI CTAG 3 cut(s) 584, 1026, 1448
SstI GAGCTC 1 cut(s) 66
StuI AGGCCT 1 cut(s) 1568
StyD4I CCNGG 2 cut(s) 854, 987
StyI CCWWGG 2 cut(s) 1353, 1505
TaaI ACNGT 2 cut(s) 34, 961
TaqI TCGA 6 cut(s) 66, 243, 288, 331, 423, 545
TatI WGTACW 1 cut(s) 1095
TauI GCSGC 3 cut(s) 113, 161, 503
TfiI GAWTC 3 cut(s) 26, 407, 795
Tru1I TTAA 2 cut(s) 1322, 1575
Tru9I TTAA 2 cut(s) 1322, 1575
TscAI CASTG 2 cut(s) 928, 1042
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 7 cut(s) 161, 1295, 1343, 1358, 1367, 1370, 1406
Tsp45I GTSAC 1 cut(s) 458
TspDTI ATGAA 3 cut(s) 296, 989, 1178
TspGWI ACGGA 2 cut(s) 792, 1406
TspRI CASTG 2 cut(s) 928, 1042
VpaK11BI GGWCC 1 cut(s) 535
XceI RCATGY 1 cut(s) 1093
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 3 cut(s) 584, 1026, 1448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.