Rroxscaffold_1G00029020

At3g49055-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005360
Physical Location & Seq
Reverse (-)
37480654 .. 37481057
404 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029020.1

Sequence Viewer

Length: 369 bp
ATGGAACCCGAAACTAAAATCAAAGGCCCGGATCCGCCGAGATCCACACAAACCTCCGATCATGATTTCCTCAACACCGAGCTCGAGTCCTTTCGCCGGTCTTACCGGTGCCTTCTCTCGAAATCAGCGATGATGGAGGAAGACTTCAATTGCCTCCAAAACCGAGAGAGACAAGGCGAAAACTTGCCGGGGAATTCTCTCCATGATGAGTTTCGCAAGCTCGAAGCTATTTCGAGAGAGAAGGAAGACAAGTATGTGATGAAAATTGATGAGGAGGTGAGAAGGAATGAGAGTTTGGAAGGTGATATTCGAGTTCGTATGGAGAGAAATGAGAAATTAGAGATTAAGAGGAAAGAAAAAGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

122

Amino Acids

14.64

Weight (kDa)

5.29

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 108
AciI CCGC 1 cut(s) 35
AclWI GGATC 3 cut(s) 26, 36, 39
AcsI RAATTY 1 cut(s) 193
AfiI CCNNNNNNNGG 1 cut(s) 96
AgeI ACCGGT 1 cut(s) 105
AgsI TTSAA 1 cut(s) 148
AjuI GAANNNNNNNTTGG 2 cut(s) 278, 310
AluBI AGCT 3 cut(s) 82, 220, 227
AluI AGCT 3 cut(s) 82, 220, 227
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 3 cut(s) 26, 36, 39
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 193
AsiGI ACCGGT 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 2 cut(s) 29, 189
AsuHPI GGTGA 2 cut(s) 289, 314
AvaI CYCGRG 1 cut(s) 83
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 108
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 2 cut(s) 147, 252
Bbv12I GWGCWC 1 cut(s) 84
BccI CCATC 1 cut(s) 127
BcnI CCSGG 2 cut(s) 29, 189
BcoDI GTCTC 1 cut(s) 163
Bme1390I CCNGG 2 cut(s) 29, 189
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 3 cut(s) 6, 33, 110
BmrFI CCNGG 2 cut(s) 29, 189
BpiI GAAGAC 2 cut(s) 147, 252
BpuMI CCSGG 2 cut(s) 29, 189
BsaJI CCNNGG 1 cut(s) 188
BsaWI WCCGGW 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse118I RCCGGY 2 cut(s) 96, 105
BseDI CCNNGG 1 cut(s) 188
BseLI CCNNNNNNNGG 1 cut(s) 96
BseRI GAGGAG 1 cut(s) 287
BshFI GGCC 1 cut(s) 27
BshNI GGYRCC 1 cut(s) 108
BshTI ACCGGT 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 4 cut(s) 29, 97, 106, 188
BslI CCNNNNNNNGG 1 cut(s) 96
BsmAI GTCTC 1 cut(s) 163
BsnI GGCC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 31, 41, 58
BspACI CCGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 27
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 3 cut(s) 6, 33, 110
BspPI GGATC 3 cut(s) 26, 36, 39
BspT107I GGYRCC 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 96, 105
BssAI RCCGGY 2 cut(s) 96, 105
BssECI CCNNGG 1 cut(s) 188
BssMI GATC 3 cut(s) 31, 41, 58
BstC8I GCNNGC 1 cut(s) 218
BstKTI GATC 3 cut(s) 34, 44, 61
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 3 cut(s) 31, 41, 58
BstSCI CCNGG 2 cut(s) 27, 187
BstV2I GAAGAC 2 cut(s) 147, 252
BstX2I RGATCY 2 cut(s) 31, 41
BstYI RGATCY 2 cut(s) 31, 41
BsuRI GGCC 1 cut(s) 27
BtgZI GCGATG 1 cut(s) 143
Cac8I GCNNGC 1 cut(s) 218
CciI TCATGA 1 cut(s) 61
Cfr10I RCCGGY 2 cut(s) 96, 105
Cfr13I GGNCC 1 cut(s) 26
CspAI ACCGGT 1 cut(s) 105
CviAII CATG 2 cut(s) 62, 203
CviJI RGCY 4 cut(s) 27, 82, 220, 227
CviKI_1 RGCY 4 cut(s) 27, 82, 220, 227
DpnI GATC 3 cut(s) 33, 43, 60
DpnII GATC 3 cut(s) 31, 41, 58
EciI GGCGGA 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 2 cut(s) 65, 206
FaiI YATR 4 cut(s) 63, 204, 255, 320
FatI CATG 2 cut(s) 61, 202
FriOI GRGCYC 1 cut(s) 84
HaeIII GGCC 1 cut(s) 27
HapII CCGG 4 cut(s) 29, 97, 106, 188
Hin1II CATG 2 cut(s) 65, 206
HinfI GANTC 1 cut(s) 86
HpaII CCGG 4 cut(s) 29, 97, 106, 188
HphI GGTGA 2 cut(s) 289, 314
Hpy188I TCNGA 1 cut(s) 58
Hpy188III TCNNGA 3 cut(s) 62, 118, 234
HpyAV CCTTC 4 cut(s) 122, 235, 276, 293
Hsp92II CATG 2 cut(s) 65, 206
Kzo9I GATC 3 cut(s) 31, 41, 58
LpnPI CCDG 4 cut(s) 42, 110, 119, 201
MalI GATC 3 cut(s) 33, 43, 60
MboI GATC 3 cut(s) 31, 41, 58
MboII GAAGA 2 cut(s) 152, 257
MfeI CAATTG 1 cut(s) 148
MflI RGATCY 2 cut(s) 31, 41
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 4 cut(s) 148, 193, 264, 335
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 7 cut(s) 64, 80, 130, 164, 265, 268, 342
MseI TTAA 1 cut(s) 345
MspI CCGG 4 cut(s) 29, 97, 106, 188
MspR9I CCNGG 2 cut(s) 29, 189
MunI CAATTG 1 cut(s) 148
NciI CCSGG 2 cut(s) 29, 189
NdeII GATC 3 cut(s) 31, 41, 58
NlaIII CATG 2 cut(s) 65, 206
NlaIV GGNNCC 3 cut(s) 6, 33, 110
NmeAIII GCCGAG 1 cut(s) 63
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 105
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
Psp124BI GAGCTC 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 6, 33, 110
PspPI GGNCC 1 cut(s) 26
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 2 cut(s) 31, 41
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 345
Sau3AI GATC 3 cut(s) 31, 41, 58
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 29, 189
SduI GDGCHC 1 cut(s) 84
SetI ASST 6 cut(s) 56, 84, 222, 229, 279, 304
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 4 cut(s) 148, 193, 264, 335
SsiI CCGC 1 cut(s) 35
SstI GAGCTC 1 cut(s) 84
StyD4I CCNGG 2 cut(s) 27, 187
TaqI TCGA 5 cut(s) 84, 119, 222, 233, 310
TasI AATT 4 cut(s) 148, 193, 264, 335
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 275
XapI RAATTY 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.