Rmu_sc0036419.1_g000001

At3g49055-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0036419.1
OG0005360
Physical Location & Seq
Reverse (-)
2 .. 417
416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0036419.1_g000001.1.cds

Sequence Viewer

Length: 416 bp
atggaaaccgaaatcaacggttcagattccacacaaacctccgatcatgatctcctccgcgccgagctcgactccctccgccggtctcaccggagcctcctacagaagacggcggcgatggaggaagatttcaaccgcgtccagagccagcgagacgaggcggcggctcgcaacgccaagttgaccagactcgtcggagaagtttccggcgagagggacgctctaagggaagagcttcgcaaggtcgaagacgagtatgcgatgaaaatcgatgaggaggtgaggaggaaggagagtctggagagtgaggttcgagtttgtaaggagagaattgaaaggttggagattgagaggagagaaagagatgagtttttgatcaagtgcttggattcgctccggccgatcgagaaggagtt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

16.29

Weight (kDa)

5.06

Isoelectric Point (pI)

73.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 60, 138
AciI CCGC 6 cut(s) 58, 79, 113, 136, 161, 164
AcoI YGGCCR 1 cut(s) 398
AfiI CCNNNNNNNGG 2 cut(s) 81, 213
AgsI TTSAA 2 cut(s) 133, 335
AluBI AGCT 2 cut(s) 67, 235
AluI AGCT 2 cut(s) 67, 235
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 2 cut(s) 90, 147
AoxI GGCC 1 cut(s) 398
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 80, 292
BanII GRGCYC 1 cut(s) 69
BbsI GAAGAC 2 cut(s) 113, 255
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 1 cut(s) 112
BceAI ACGGC 1 cut(s) 126
BclI TGATCA 1 cut(s) 375
BcoDI GTCTC 2 cut(s) 90, 147
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 3 cut(s) 114, 162, 165
BlsI GCNGC 3 cut(s) 115, 163, 166
BmiI GGNNCC 1 cut(s) 95
BpiI GAAGAC 2 cut(s) 113, 255
BplI GAGNNNNNCTC 4 cut(s) 56, 88, 205, 237
BpmI CTGGAG 1 cut(s) 320
Bsa29I ATCGAT 1 cut(s) 270
BsaBI GATNNNNATC 2 cut(s) 48, 266
BsaI GGTCTC 1 cut(s) 90
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 213
Bse118I RCCGGY 1 cut(s) 81
Bse8I GATNNNNATC 2 cut(s) 48, 266
BseCI ATCGAT 1 cut(s) 270
BseJI GATNNNNATC 2 cut(s) 48, 266
BseLI CCNNNNNNNGG 2 cut(s) 81, 213
BseRI GAGGAG 4 cut(s) 44, 290, 298, 367
BseX3I CGGCCG 1 cut(s) 398
Bsh1236I CGCG 2 cut(s) 60, 138
Bsh1285I CGRYCG 2 cut(s) 401, 405
BshFI GGCC 1 cut(s) 400
BshVI ATCGAT 1 cut(s) 270
BsiEI CGRYCG 2 cut(s) 401, 405
BsiHKAI GWGCWC 1 cut(s) 69
BsiSI CCGG 4 cut(s) 82, 91, 207, 397
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 2 cut(s) 81, 213
BsmAI GTCTC 2 cut(s) 90, 147
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 1 cut(s) 400
Bso31I GGTCTC 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 4 cut(s) 43, 49, 375, 402
BspACI CCGC 6 cut(s) 58, 79, 113, 136, 161, 164
BspANI GGCC 1 cut(s) 400
BspDI ATCGAT 1 cut(s) 270
BspFNI CGCG 2 cut(s) 60, 138
BspHI TCATGA 1 cut(s) 46
BspLI GGNNCC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 225
BspTNI GGTCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 81
BssMI GATC 4 cut(s) 43, 49, 375, 402
Bst4CI ACNGT 1 cut(s) 20
Bst6I CTCTTC 1 cut(s) 225
BstC8I GCNNGC 2 cut(s) 149, 169
BstDEI CTNAG 1 cut(s) 224
BstFNI CGCG 2 cut(s) 60, 138
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 4 cut(s) 46, 52, 378, 405
BstMAI GTCTC 2 cut(s) 90, 147
BstMBI GATC 4 cut(s) 43, 49, 375, 402
BstMCI CGRYCG 2 cut(s) 401, 405
BstMWI GCNNNNNNNGC 2 cut(s) 144, 173
BstSFI CTRYAG 1 cut(s) 101
BstUI CGCG 2 cut(s) 60, 138
BstV2I GAAGAC 2 cut(s) 113, 255
BstZI CGGCCG 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 270
BsuRI GGCC 1 cut(s) 400
BsuTUI ATCGAT 1 cut(s) 270
BtgZI GCGATG 2 cut(s) 131, 275
Cac8I GCNNGC 2 cut(s) 149, 169
CciI TCATGA 1 cut(s) 46
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 1 cut(s) 81
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 2 cut(s) 127, 227
CviAII CATG 1 cut(s) 47
CviJI RGCY 6 cut(s) 67, 96, 147, 167, 235, 400
CviKI_1 RGCY 6 cut(s) 67, 96, 147, 167, 235, 400
DdeI CTNAG 1 cut(s) 224
DpnI GATC 4 cut(s) 45, 51, 377, 404
DpnII GATC 4 cut(s) 43, 49, 375, 402
EaeI YGGCCR 1 cut(s) 398
EagI CGGCCG 1 cut(s) 398
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 67
EclXI CGGCCG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 69
Eco31I GGTCTC 1 cut(s) 90
Eco52I CGGCCG 1 cut(s) 398
Eco53kI GAGCTC 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 69
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 1 cut(s) 50
FaiI YATR 2 cut(s) 48, 258
FaqI GGGAC 1 cut(s) 230
FatI CATG 1 cut(s) 46
FbaI TGATCA 1 cut(s) 375
Fnu4HI GCNGC 3 cut(s) 114, 162, 165
FriOI GRGCYC 1 cut(s) 69
Fsp4HI GCNGC 3 cut(s) 114, 162, 165
GlaI GCGC 1 cut(s) 61
GluI GCNGC 3 cut(s) 114, 162, 165
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 1 cut(s) 400
HapII CCGG 4 cut(s) 82, 91, 207, 397
HgaI GACGC 2 cut(s) 127, 227
HhaI GCGC 1 cut(s) 62
Hin1II CATG 1 cut(s) 50
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HinfI GANTC 5 cut(s) 26, 71, 189, 295, 389
HpaII CCGG 4 cut(s) 82, 91, 207, 397
HphI GGTGA 2 cut(s) 80, 292
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 3 cut(s) 25, 43, 197
Hpy188III TCNNGA 4 cut(s) 47, 142, 299, 406
Hpy8I GTNNAC 1 cut(s) 183
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 2 cut(s) 283, 403
HpyCH4III ACNGT 1 cut(s) 20
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 173
HpyF3I CTNAG 1 cut(s) 224
Hsp92II CATG 1 cut(s) 50
HspAI GCGC 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 375
Kzo9I GATC 4 cut(s) 43, 49, 375, 402
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 2 cut(s) 93, 399
LpnPI CCDG 8 cut(s) 95, 104, 155, 161, 199, 220, 284, 410
MalI GATC 4 cut(s) 45, 51, 377, 404
MboI GATC 4 cut(s) 43, 49, 375, 402
MboII GAAGA 4 cut(s) 118, 137, 242, 260
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 330
MlyI GAGTC 3 cut(s) 65, 183, 304
MmeI TCCRAC 2 cut(s) 175, 321
MroXI GAANNNNTTC 1 cut(s) 234
MspI CCGG 4 cut(s) 82, 91, 207, 397
MvnI CGCG 2 cut(s) 60, 138
MwoI GCNNNNNNNGC 2 cut(s) 144, 173
NdeII GATC 4 cut(s) 43, 49, 375, 402
NlaIII CATG 1 cut(s) 50
NlaIV GGNNCC 1 cut(s) 95
NmeAIII GCCGAG 1 cut(s) 88
PagI TCATGA 1 cut(s) 46
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 2 cut(s) 26, 389
PkrI GCNGC 3 cut(s) 115, 163, 166
Ple19I CGATCG 1 cut(s) 405
PleI GAGTC 3 cut(s) 65, 183, 303
PpsI GAGTC 3 cut(s) 65, 183, 303
Psp124BI GAGCTC 1 cut(s) 69
PspN4I GGNNCC 1 cut(s) 95
PvuI CGATCG 1 cut(s) 405
SacI GAGCTC 1 cut(s) 69
SapI GCTCTTC 1 cut(s) 225
SatI GCNGC 3 cut(s) 114, 162, 165
Sau3AI GATC 4 cut(s) 43, 49, 375, 402
SchI GAGTC 3 cut(s) 65, 183, 304
SduI GDGCHC 1 cut(s) 69
SetI ASST 7 cut(s) 41, 69, 237, 246, 282, 312, 341
SfcI CTRYAG 1 cut(s) 101
Sse9I AATT 1 cut(s) 330
SsiI CCGC 6 cut(s) 58, 79, 113, 136, 161, 164
SstI GAGCTC 1 cut(s) 69
TaaI ACNGT 1 cut(s) 20
TaqI TCGA 5 cut(s) 69, 246, 270, 313, 405
TasI AATT 1 cut(s) 330
TauI GCSGC 3 cut(s) 116, 164, 167
TfiI GAWTC 2 cut(s) 26, 389
TspDTI ATGAA 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.