Rh5DG364900

At3g49055-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005360
Physical Location & Seq
Forward (+)
54627058 .. 54627477
420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG364900.1

Sequence Viewer

Length: 351 bp
ATGGAACCTGAAACTAAAATCAAAGGCCCAGATCCGCCTAGATCCACACAAACCTTCAATCATGATCTCCTCAACACCGAGCTCGAGTCCTTTCGCCAGTCTTACCAGCACCTTCTCTCGAAGTCAGCGACGATGGAGGAAGACTTCAATTGCCTCCAAAACCAGAGAGACAAGGCAACGGCTCGCAACGGTGAGTTAACCAGAGTCATCGAAGAAGTTTCTAGTGAGAGGAATTCTTGTCGCAATGAGCTTCGCAAGCTCAAAGCTATTTTGAGAGAGAAGGAAGACAAGTATGTGATCAAAATTGATGAGGAGGTGAGAAGGAAGGAGAGTTTGGAAGGTGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

13.62

Weight (kDa)

5.53

Isoelectric Point (pI)

56.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 35
AclWI GGATC 2 cut(s) 26, 36
AcsI RAATTY 1 cut(s) 232
AgsI TTSAA 2 cut(s) 58, 148
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 4 cut(s) 82, 250, 259, 266
AluI AGCT 4 cut(s) 82, 250, 259, 266
Alw21I GWGCWC 1 cut(s) 84
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 2 cut(s) 26, 36
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 26
AsuHPI GGTGA 2 cut(s) 203, 328
AvaI CYCGRG 1 cut(s) 83
BanII GRGCYC 1 cut(s) 84
BbsI GAAGAC 2 cut(s) 147, 291
Bbv12I GWGCWC 1 cut(s) 84
BccI CCATC 1 cut(s) 127
BceAI ACGGC 1 cut(s) 195
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 2 cut(s) 39, 222
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 2 cut(s) 147, 291
Bse1I ACTGG 1 cut(s) 97
Bse3DI GCAATG 1 cut(s) 250
BseMI GCAATG 1 cut(s) 250
BseNI ACTGG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 59, 326
BshFI GGCC 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 162
BsnI GGCC 1 cut(s) 27
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 4 cut(s) 31, 41, 64, 297
BspACI CCGC 1 cut(s) 35
BspANI GGCC 1 cut(s) 27
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 26, 36
BsrDI GCAATG 1 cut(s) 250
BsrI ACTGG 1 cut(s) 97
BssMI GATC 4 cut(s) 31, 41, 64, 297
Bst4CI ACNGT 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 184, 257
BstKTI GATC 4 cut(s) 34, 44, 67, 300
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 4 cut(s) 31, 41, 64, 297
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstV2I GAAGAC 2 cut(s) 147, 291
BstX2I RGATCY 2 cut(s) 31, 41
BstYI RGATCY 2 cut(s) 31, 41
BsuRI GGCC 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 184, 257
CciI TCATGA 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 26
CviAII CATG 1 cut(s) 62
CviJI RGCY 6 cut(s) 27, 82, 182, 250, 259, 266
CviKI_1 RGCY 6 cut(s) 27, 82, 182, 250, 259, 266
DpnI GATC 4 cut(s) 33, 43, 66, 299
DpnII GATC 4 cut(s) 31, 41, 64, 297
EciI GGCGGA 1 cut(s) 24
Ecl136II GAGCTC 1 cut(s) 82
Eco24I GRGCYC 1 cut(s) 84
Eco53kI GAGCTC 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 232
EcoT38I GRGCYC 1 cut(s) 84
FaeI CATG 1 cut(s) 65
FaiI YATR 2 cut(s) 63, 294
FatI CATG 1 cut(s) 61
FbaI TGATCA 1 cut(s) 297
FriOI GRGCYC 1 cut(s) 84
FspBI CTAG 2 cut(s) 39, 222
HaeIII GGCC 1 cut(s) 27
Hin1II CATG 1 cut(s) 65
HincII GTYRAC 1 cut(s) 198
HindII GTYRAC 1 cut(s) 198
HinfI GANTC 2 cut(s) 86, 204
HpaI GTTAAC 1 cut(s) 198
HphI GGTGA 2 cut(s) 203, 328
Hpy166II GTNNAC 1 cut(s) 198
Hpy188III TCNNGA 2 cut(s) 62, 118
Hpy8I GTNNAC 1 cut(s) 198
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 6 cut(s) 64, 122, 274, 315, 319, 332
HpyCH4III ACNGT 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
Hsp92II CATG 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 198
Kzo9I GATC 4 cut(s) 31, 41, 64, 297
LpnPI CCDG 6 cut(s) 21, 42, 110, 119, 176, 214
MaeI CTAG 2 cut(s) 39, 222
MalI GATC 4 cut(s) 33, 43, 66, 299
MboI GATC 4 cut(s) 31, 41, 64, 297
MboII GAAGA 3 cut(s) 152, 224, 296
MfeI CAATTG 1 cut(s) 148
MflI RGATCY 2 cut(s) 31, 41
MhlI GDGCHC 1 cut(s) 84
MluCI AATT 3 cut(s) 148, 232, 303
MlyI GAGTC 2 cut(s) 95, 213
MnlI CCTC 6 cut(s) 80, 130, 164, 222, 304, 307
MseI TTAA 1 cut(s) 197
MunI CAATTG 1 cut(s) 148
MwoI GCNNNNNNNGC 1 cut(s) 256
NdeII GATC 4 cut(s) 31, 41, 64, 297
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 83
PagI TCATGA 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PleI GAGTC 2 cut(s) 94, 212
PpsI GAGTC 2 cut(s) 94, 212
Psp124BI GAGCTC 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 26
PspXI VCTCGAGB 1 cut(s) 83
PsuI RGATCY 2 cut(s) 31, 41
SacI GAGCTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 197
Sau3AI GATC 4 cut(s) 31, 41, 64, 297
Sau96I GGNCC 1 cut(s) 26
SchI GAGTC 2 cut(s) 95, 213
SduI GDGCHC 1 cut(s) 84
SetI ASST 9 cut(s) 10, 56, 84, 114, 252, 261, 268, 318, 343
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 83
SmoI CTYRAG 1 cut(s) 83
Sse9I AATT 3 cut(s) 148, 232, 303
SsiI CCGC 1 cut(s) 35
SspMI CTAG 2 cut(s) 39, 222
SstI GAGCTC 1 cut(s) 84
TaaI ACNGT 1 cut(s) 191
TaqI TCGA 3 cut(s) 84, 119, 210
TasI AATT 3 cut(s) 148, 232, 303
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
XapI RAATTY 1 cut(s) 232
XhoI CTCGAG 1 cut(s) 83
XspI CTAG 2 cut(s) 39, 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.