pycom01g22580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004464
Physical Location & Seq
Reverse (-)
20118817 .. 20122519
3703 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22580.1

Sequence Viewer

Length: 1905 bp
ATGATAAGCTCGGAGTCCTCTGAGCGCGCGCAAAAGCCTCTTTCGGGCCCCACATTTCTCATAAGCCCGACTCCAGTCGAAGAAATTAAGAGTGAGTATGAACCTCTGGATGCCAAGTTTCGGGCACTGGAACGCGAAAAGATTGCGGTGGAAGAAGAGATTAAGGCTTTGAAGAGGGAGAGTGGTGGGGATGAAAAGAAGAAAACTTGTGGGGGAGAAAATGGGGAGGGAATTGGTGATCTGACGGAGGAGAACGAGGAGGAAGATGAGGTCTTTCAGCTTATGGTTGAGATTGAGGTATTGGAGTGTGAGAAGAAAAAGGCTGAAAGCGATGTTGAGGCTTGGAAGCAAAAGTTCAAGGACCTGGAATTGATGATGCTAAAGTTGGACAAGAAATCCGTCTTGAAAGGCGGGGAATTGCCGCTGGCTGGAAGGATGAAGGTGGCAGGGATAGGATCGCCAATTGTGGATTCTCTTGAAGGCAGCGGGACCGTGCAACTGAATAACAGGATTAAACTTCCAGATGAATTGCAAGTTGAAGGTGGCTTTGAGTATTTGAGAGACAAAGATATGGCTGTAGATGTGGTGGATGTTCGCTCTGTATGTCTCCCCCTTGGTAATGAGATCGGCAATACGCAGTCAGCAGGCACTCCGTGCAATGACACACCGTGTAAGCATTGGGATGGTATTGAGGGAGAGAAGAAAGGTGTTTGTGTAGAGCCTGATATGGAATATTCTAAAAGTAGACAAGCTAGGAAACGGTTGGCATTTGACAAAGAGGGGAGTCCTTGCAAGAAGATGGCTCCATCCACGCCTGGTGGTGGCATACCTTCTTCTCTCGCTGTCGTCAATATTAGTGATAGTGATGATGAACTTTTGCCTGCAGATAATCAGAAAAGCAGAAAGGTCACCATTTTGTCGGGTTCAGTATTAGGAGAAACTTTGGGTTGTGAAATGGACATGACTACAAAAAACGGTGAGCGGACACATCCTGAACAAAATGTTGATGAAGATACTGATAATATCAATGAGGTTTTTCTGCCTGCCTCAAAACCCAATAGGAAACGTGCTCTTAATATTGTTACTAGTGACTCTGAAAGTAGTGATGATGATATTCCGATTGCAAAACTCAAGGAAATGCATTTTAAGGAAGTTATACGTGACAAAGTAGGATCTGATCTCAATGACAGCTCAGTGACTGCTACTGCTTCTGAGGTTGACAATGCCATGGGTACTGTCAGTTCTCCCAGGCGGCATCTTGTTAGCTTGAGGAAATGTGGGGAAAGAGGCAGTGCACGAGAAGACTATTCAACTCAGACTAGTGAAACTAAATATGATCAAGAAAATCTGGCTAATGAGGATGTTGAAGATGATGACTTGGAAGAGGTTGGGTCGGAAAGTGAAGGTGAAAGCTTGGGTGGATTTATTGTTCAAGATGATTCTGATGTTTCTGATGCTCATGATGCTTCTACAAAGTCAGAAGGTTCCTCAGATGATGATGTGGACTTTGATGAGATTCTATCAAAATTTCAGAGGAACAAAGATCAGAAATCGAAGTGGGAATTTGAGGCAGACATGCTTTCTGCATTTGGTAAGGATCCGGAGCTGTGCATGAAAGCTGTATGTGCTCTTTATCGACAGCAGACTTCTGAAGAACAATATAGCAAGGAATCATTGTGCACCAATAGTCGTGGTTTTAGCAAGTTCGATGCTTTCAAGGGATCTTTATTGGCCGAGTTTCTCACCAATGGAGACCCGAACGAAGATCTCAATAGAACTGTGGAGGAGTTGCAGGCATATGACGCGAACGGCATTGAAATGTGTAGAACATTGGCGAATCGTTACTCTAAGCAACTGTTCGAAATCTACAAGAACGAAGAGGATCCTCTCTTTCTACCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

635

Amino Acids

70.18

Weight (kDa)

4.53

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 982
AccI GTMKAC 1 cut(s) 745
AccII CGCG 4 cut(s) 27, 29, 135, 1805
AccIII TCCGGA 1 cut(s) 1600
AciI CCGC 6 cut(s) 146, 411, 422, 486, 982, 1252
AclWI GGATC 7 cut(s) 463, 1180, 1592, 1605, 1729, 1877, 1890
AcoI YGGCCR 1 cut(s) 1731
AcsI RAATTY 2 cut(s) 1526, 1562
AcuI CTGAAG 1 cut(s) 1671
AdeI CACNNNGTG 2 cut(s) 654, 669
AfaI GTAC 1 cut(s) 1234
AfiI CCNNNNNNNGG 4 cut(s) 44, 120, 428, 821
AhlI ACTAGT 2 cut(s) 1085, 1319
AjnI CCWGG 3 cut(s) 363, 814, 1247
AjuI GAANNNNNNNTTGG 2 cut(s) 454, 486
AloI GAACNNNNNNTCC 2 cut(s) 1413, 1445
AluBI AGCT 8 cut(s) 9, 280, 752, 1191, 1266, 1413, 1606, 1619
AluI AGCT 8 cut(s) 9, 280, 752, 1191, 1266, 1413, 1606, 1619
Alw21I GWGCWC 4 cut(s) 1072, 1297, 1630, 1682
Alw26I GTCTC 3 cut(s) 555, 611, 1746
Alw44I GTGCAC 2 cut(s) 1293, 1678
AlwI GGATC 7 cut(s) 463, 1180, 1592, 1605, 1729, 1877, 1890
AlwNI CAGNNNCTG 1 cut(s) 1199
Aor13HI TCCGGA 1 cut(s) 1600
AoxI GGCC 2 cut(s) 46, 1731
ApaI GGGCCC 1 cut(s) 50
ApaLI GTGCAC 2 cut(s) 1293, 1678
ApeKI GCWGC 1 cut(s) 483
ApoI RAATTY 2 cut(s) 1526, 1562
AspLEI GCGC 3 cut(s) 27, 29, 31
AspS9I GGNCC 4 cut(s) 46, 47, 361, 489
AsuHPI GGTGA 5 cut(s) 248, 901, 989, 1418, 1735
AsuII TTCGAA 1 cut(s) 1860
AvaII GGWCC 2 cut(s) 361, 489
BaeGI GKGCMC 4 cut(s) 50, 127, 1297, 1682
BamHI GGATCC 2 cut(s) 1597, 1882
BanII GRGCYC 1 cut(s) 50
BauI CACGAG 1 cut(s) 1296
BbsI GAAGAC 1 cut(s) 1308
Bbv12I GWGCWC 4 cut(s) 1072, 1297, 1630, 1682
BbvI GCAGC 1 cut(s) 495
BccI CCATC 3 cut(s) 677, 793, 814
BceAI ACGGC 1 cut(s) 1825
BcgI CGANNNNNNTGC 2 cut(s) 125, 159
BciT130I CCWGG 3 cut(s) 365, 816, 1249
BclI TGATCA 1 cut(s) 1336
BcoDI GTCTC 3 cut(s) 555, 611, 1746
BcuI ACTAGT 2 cut(s) 1085, 1319
BfaI CTAG 3 cut(s) 753, 1086, 1320
BfmI CTRYAG 2 cut(s) 576, 882
BglII AGATCT 1 cut(s) 1765
BisI GCNGC 3 cut(s) 422, 484, 1253
BlsI GCNGC 3 cut(s) 423, 485, 1254
Bme1390I CCNGG 3 cut(s) 365, 816, 1249
Bme18I GGWCC 2 cut(s) 361, 489
BmgT120I GGNCC 4 cut(s) 46, 47, 361, 489
BmiI GGNNCC 7 cut(s) 48, 49, 490, 804, 1486, 1599, 1884
BmrFI CCNGG 3 cut(s) 365, 816, 1249
BmsI GCATC 6 cut(s) 100, 366, 1264, 1444, 1453, 1699
BoxI GACNNNNGTC 1 cut(s) 74
BpiI GAAGAC 1 cut(s) 1308
BpmI CTGGAG 1 cut(s) 57
Bpu14I TTCGAA 1 cut(s) 1860
BpuEI CTTGAG 2 cut(s) 1115, 1288
BsaAI YACGTR 1 cut(s) 1160
BsaI GGTCTC 1 cut(s) 1746
BsaJI CCNNGG 3 cut(s) 613, 1227, 1247
BsaWI WCCGGW 1 cut(s) 1600
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 120, 428, 821
Bse1I ACTGG 2 cut(s) 74, 132
Bse3DI GCAATG 1 cut(s) 664
BseAI TCCGGA 1 cut(s) 1600
BseBI CCWGG 3 cut(s) 365, 816, 1249
BseDI CCNNGG 3 cut(s) 613, 1227, 1247
BseGI GGATG 8 cut(s) 115, 196, 441, 595, 688, 806, 988, 1366
BseLI CCNNNNNNNGG 4 cut(s) 44, 120, 428, 821
BseMI GCAATG 1 cut(s) 664
BseMII CTCAG 5 cut(s) 12, 1203, 1206, 1328, 1503
BseNI ACTGG 2 cut(s) 74, 132
BsePI GCGCGC 2 cut(s) 25, 27
BseRI GAGGAG 3 cut(s) 263, 272, 1799
BseSI GKGCMC 4 cut(s) 50, 127, 1297, 1682
BseXI GCAGC 1 cut(s) 495
Bsh1236I CGCG 4 cut(s) 27, 29, 135, 1805
BshFI GGCC 2 cut(s) 48, 1733
BsiHKAI GWGCWC 4 cut(s) 1072, 1297, 1630, 1682
BsiSI CCGG 1 cut(s) 1601
BslFI GGGAC 1 cut(s) 502
BslI CCNNNNNNNGG 4 cut(s) 44, 120, 428, 821
BsmAI GTCTC 3 cut(s) 555, 611, 1746
BsmFI GGGAC 1 cut(s) 502
BsnI GGCC 2 cut(s) 48, 1733
Bso31I GGTCTC 1 cut(s) 1746
Bsp119I TTCGAA 1 cut(s) 1860
Bsp120I GGGCCC 1 cut(s) 46
Bsp1286I GDGCHC 6 cut(s) 50, 127, 1072, 1297, 1630, 1682
Bsp13I TCCGGA 1 cut(s) 1600
Bsp19I CCATGG 1 cut(s) 1227
BspACI CCGC 6 cut(s) 146, 411, 422, 486, 982, 1252
BspANI GGCC 2 cut(s) 48, 1733
BspCNI CTCAG 5 cut(s) 13, 1204, 1205, 1327, 1502
BspEI TCCGGA 1 cut(s) 1600
BspFNI CGCG 4 cut(s) 27, 29, 135, 1805
BspHI TCATGA 1 cut(s) 1459
BspLI GGNNCC 7 cut(s) 48, 49, 490, 804, 1486, 1599, 1884
BspMAI CTGCAG 1 cut(s) 886
BspPI GGATC 7 cut(s) 463, 1180, 1592, 1605, 1729, 1877, 1890
BspT104I TTCGAA 1 cut(s) 1860
BspTNI GGTCTC 1 cut(s) 1746
BsrBI CCGCTC 1 cut(s) 982
BsrDI GCAATG 1 cut(s) 664
BsrI ACTGG 2 cut(s) 74, 132
BssECI CCNNGG 3 cut(s) 613, 1227, 1247
BssHII GCGCGC 2 cut(s) 25, 27
BssSI CACGAG 1 cut(s) 1296
BssT1I CCWWGG 2 cut(s) 613, 1227
Bst2BI CACGAG 1 cut(s) 1296
Bst2UI CCWGG 3 cut(s) 365, 816, 1249
Bst4CI ACNGT 7 cut(s) 493, 669, 762, 977, 1237, 1780, 1857
Bst6I CTCTTC 4 cut(s) 150, 167, 1377, 1872
BstAPI GCANNNNNTGC 1 cut(s) 654
BstBAI YACGTR 1 cut(s) 1160
BstBI TTCGAA 1 cut(s) 1860
BstC8I GCNNGC 7 cut(s) 27, 29, 426, 646, 882, 1044, 1794
BstDEI CTNAG 6 cut(s) 21, 1192, 1212, 1314, 1489, 1848
BstDSI CCRYGG 1 cut(s) 1227
BstEII GGTNACC 1 cut(s) 907
BstF5I GGATG 8 cut(s) 115, 196, 441, 595, 688, 806, 988, 1366
BstFNI CGCG 4 cut(s) 27, 29, 135, 1805
BstHHI GCGC 3 cut(s) 27, 29, 31
BstMAI GTCTC 3 cut(s) 555, 611, 1746
BstMWI GCNNNNNNNGC 4 cut(s) 654, 1463, 1625, 1802
BstNI CCWGG 3 cut(s) 365, 816, 1249
BstNSI RCATGY 1 cut(s) 1579
BstPAI GACNNNNGTC 1 cut(s) 74
BstPI GGTNACC 1 cut(s) 907
BstSCI CCNGG 3 cut(s) 363, 814, 1247
BstSFI CTRYAG 2 cut(s) 576, 882
BstSLI GKGCMC 4 cut(s) 50, 127, 1297, 1682
BstUI CGCG 4 cut(s) 27, 29, 135, 1805
BstV1I GCAGC 1 cut(s) 495
BstV2I GAAGAC 1 cut(s) 1308
BstX2I RGATCY 5 cut(s) 1172, 1597, 1721, 1765, 1882
BstYI RGATCY 5 cut(s) 1172, 1597, 1721, 1765, 1882
BsuRI GGCC 2 cut(s) 48, 1733
BtgI CCRYGG 1 cut(s) 1227
BtgZI GCGATG 1 cut(s) 345
BtsCI GGATG 8 cut(s) 115, 196, 441, 595, 688, 806, 988, 1366
BtsI GCAGTG 1 cut(s) 1297
BtsIMutI CAGTG 3 cut(s) 125, 1200, 1297
Cac8I GCNNGC 7 cut(s) 27, 29, 426, 646, 882, 1044, 1794
CaiI CAGNNNCTG 1 cut(s) 1199
CciI TCATGA 1 cut(s) 1459
CfoI GCGC 3 cut(s) 27, 29, 31
Cfr13I GGNCC 4 cut(s) 46, 47, 361, 489
CseI GACGC 1 cut(s) 1811
Csp6I GTAC 1 cut(s) 1233
CspCI CAANNNNNGTGG 2 cut(s) 1672, 1707
CviAII CATG 5 cut(s) 961, 1228, 1460, 1576, 1612
CviQI GTAC 1 cut(s) 1233
DdeI CTNAG 6 cut(s) 21, 1192, 1212, 1314, 1489, 1848
DraIII CACNNNGTG 2 cut(s) 654, 669
EaeI YGGCCR 1 cut(s) 1731
Eam1104I CTCTTC 4 cut(s) 150, 167, 1377, 1872
EarI CTCTTC 4 cut(s) 150, 167, 1377, 1872
Eco130I CCWWGG 2 cut(s) 613, 1227
Eco24I GRGCYC 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 1746
Eco47I GGWCC 2 cut(s) 361, 489
Eco57I CTGAAG 1 cut(s) 1671
Eco91I GGTNACC 1 cut(s) 907
EcoO109I RGGNCCY 2 cut(s) 47, 361
EcoO65I GGTNACC 1 cut(s) 907
EcoRII CCWGG 3 cut(s) 363, 814, 1247
EcoT14I CCWWGG 2 cut(s) 613, 1227
EcoT22I ATGCAT 1 cut(s) 1143
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 2 cut(s) 613, 1227
FaeI CATG 5 cut(s) 964, 1231, 1463, 1579, 1615
FaqI GGGAC 1 cut(s) 502
FatI CATG 5 cut(s) 960, 1227, 1459, 1575, 1611
FauI CCCGC 2 cut(s) 404, 479
FauNDI CATATG 1 cut(s) 1798
FbaI TGATCA 1 cut(s) 1336
FblI GTMKAC 1 cut(s) 745
Fnu4HI GCNGC 3 cut(s) 422, 484, 1253
FokI GGATG 8 cut(s) 122, 203, 448, 602, 695, 793, 975, 1373
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 3 cut(s) 422, 484, 1253
FspBI CTAG 3 cut(s) 753, 1086, 1320
GlaI GCGC 3 cut(s) 26, 28, 30
GluI GCNGC 3 cut(s) 422, 484, 1253
GsuI CTGGAG 1 cut(s) 57
HaeIII GGCC 2 cut(s) 48, 1733
HapII CCGG 1 cut(s) 1601
HgaI GACGC 1 cut(s) 1811
HhaI GCGC 3 cut(s) 27, 29, 31
Hin1II CATG 5 cut(s) 964, 1231, 1463, 1579, 1615
Hin6I GCGC 3 cut(s) 25, 27, 29
HinP1I GCGC 3 cut(s) 25, 27, 29
HincII GTYRAC 1 cut(s) 1219
HindII GTYRAC 1 cut(s) 1219
HindIII AAGCTT 1 cut(s) 1411
HinfI GANTC 9 cut(s) 14, 70, 470, 784, 1091, 1439, 1516, 1670, 1837
HpaII CCGG 1 cut(s) 1601
HphI GGTGA 5 cut(s) 248, 901, 989, 1418, 1735
Hpy166II GTNNAC 5 cut(s) 746, 1219, 1295, 1504, 1680
Hpy188III TCNNGA 9 cut(s) 107, 403, 476, 521, 992, 1340, 1433, 1460, 1601
Hpy8I GTNNAC 5 cut(s) 746, 1219, 1295, 1504, 1680
HpyAV CCTTC 7 cut(s) 426, 433, 473, 533, 840, 1397, 1475
HpyCH4III ACNGT 7 cut(s) 493, 669, 762, 977, 1237, 1780, 1857
HpyCH4IV ACGT 2 cut(s) 1066, 1159
HpyF10VI GCNNNNNNNGC 4 cut(s) 654, 1463, 1625, 1802
HpyF3I CTNAG 6 cut(s) 21, 1192, 1212, 1314, 1489, 1848
HpySE526I ACGT 2 cut(s) 1066, 1159
Hsp92II CATG 5 cut(s) 964, 1231, 1463, 1579, 1615
HspAI GCGC 3 cut(s) 25, 27, 29
Kpn2I TCCGGA 1 cut(s) 1600
Ksp22I TGATCA 1 cut(s) 1336
LmnI GCTCC 2 cut(s) 808, 1603
Lsp1109I GCAGC 1 cut(s) 495
LweI GCATC 6 cut(s) 100, 366, 1264, 1444, 1453, 1699
MaeI CTAG 3 cut(s) 753, 1086, 1320
MaeII ACGT 2 cut(s) 1066, 1159
MaeIII GTNAC 6 cut(s) 907, 1081, 1088, 1160, 1195, 1841
MbiI CCGCTC 1 cut(s) 982
MfeI CAATTG 1 cut(s) 462
MflI RGATCY 5 cut(s) 1172, 1597, 1721, 1765, 1882
MhlI GDGCHC 6 cut(s) 50, 127, 1072, 1297, 1630, 1682
MluCI AATT 8 cut(s) 84, 231, 368, 416, 462, 527, 1526, 1562
MlyI GAGTC 4 cut(s) 23, 64, 793, 1085
MmeI TCCRAC 2 cut(s) 366, 1374
Mph1103I ATGCAT 1 cut(s) 1143
MroI TCCGGA 1 cut(s) 1600
MseI TTAA 5 cut(s) 87, 162, 513, 1074, 1146
MslI CAYNNNNRTG 2 cut(s) 681, 1817
MspA1I CMGCKG 2 cut(s) 424, 486
MspI CCGG 1 cut(s) 1601
MspR9I CCNGG 3 cut(s) 365, 816, 1249
MunI CAATTG 1 cut(s) 462
MvaI CCWGG 3 cut(s) 365, 816, 1249
MvnI CGCG 4 cut(s) 27, 29, 135, 1805
MwoI GCNNNNNNNGC 4 cut(s) 654, 1463, 1625, 1802
NcoI CCATGG 1 cut(s) 1227
NdeI CATATG 1 cut(s) 1798
NlaIII CATG 5 cut(s) 964, 1231, 1463, 1579, 1615
NlaIV GGNNCC 7 cut(s) 48, 49, 490, 804, 1486, 1599, 1884
NmeAIII GCCGAG 1 cut(s) 1759
NmuCI GTSAC 4 cut(s) 907, 1088, 1160, 1195
NsiI ATGCAT 1 cut(s) 1143
NspI RCATGY 1 cut(s) 1579
NspV TTCGAA 1 cut(s) 1860
PagI TCATGA 1 cut(s) 1459
PauI GCGCGC 2 cut(s) 25, 27
PfeI GAWTC 5 cut(s) 470, 1439, 1516, 1670, 1837
PkrI GCNGC 3 cut(s) 423, 485, 1254
PleI GAGTC 4 cut(s) 22, 64, 792, 1085
PpsI GAGTC 4 cut(s) 22, 64, 792, 1085
Ppu21I YACGTR 1 cut(s) 1160
PpuMI RGGWCCY 1 cut(s) 361
PshAI GACNNNNGTC 1 cut(s) 74
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 3 cut(s) 363, 814, 1247
PspEI GGTNACC 1 cut(s) 907
PspGI CCWGG 3 cut(s) 363, 814, 1247
PspN4I GGNNCC 7 cut(s) 48, 49, 490, 804, 1486, 1599, 1884
PspOMI GGGCCC 1 cut(s) 46
PspPI GGNCC 4 cut(s) 46, 47, 361, 489
PspPPI RGGWCCY 1 cut(s) 361
PsrI GAACNNNNNNTAC 2 cut(s) 1225, 1257
PstI CTGCAG 1 cut(s) 886
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 5 cut(s) 1172, 1597, 1721, 1765, 1882
PteI GCGCGC 2 cut(s) 25, 27
RsaI GTAC 1 cut(s) 1234
RsaNI GTAC 1 cut(s) 1233
RseI CAYNNNNRTG 2 cut(s) 681, 1817
SaqAI TTAA 5 cut(s) 87, 162, 513, 1074, 1146
SatI GCNGC 3 cut(s) 422, 484, 1253
Sau96I GGNCC 4 cut(s) 46, 47, 361, 489
SchI GAGTC 4 cut(s) 23, 64, 793, 1085
ScrFI CCNGG 3 cut(s) 365, 816, 1249
SduI GDGCHC 6 cut(s) 50, 127, 1072, 1297, 1630, 1682
SfaNI GCATC 6 cut(s) 100, 366, 1264, 1444, 1453, 1699
SfcI CTRYAG 2 cut(s) 576, 882
SfuI TTCGAA 1 cut(s) 1860
SinI GGWCC 2 cut(s) 361, 489
SmiMI CAYNNNNRTG 2 cut(s) 681, 1817
SmlI CTYRAG 2 cut(s) 1130, 1267
SmoI CTYRAG 2 cut(s) 1130, 1267
SpeI ACTAGT 2 cut(s) 1085, 1319
Sse9I AATT 8 cut(s) 84, 231, 368, 416, 462, 527, 1526, 1562
SsiI CCGC 6 cut(s) 146, 411, 422, 486, 982, 1252
SspI AATATT 3 cut(s) 734, 853, 1078
SspMI CTAG 3 cut(s) 753, 1086, 1320
StyD4I CCNGG 3 cut(s) 363, 814, 1247
StyI CCWWGG 2 cut(s) 613, 1227
TaaI ACNGT 7 cut(s) 493, 669, 762, 977, 1237, 1780, 1857
TaiI ACGT 2 cut(s) 1069, 1162
TaqI TCGA 5 cut(s) 78, 1553, 1636, 1707, 1860
TasI AATT 8 cut(s) 84, 231, 368, 416, 462, 527, 1526, 1562
TauI GCSGC 2 cut(s) 424, 1255
TfiI GAWTC 5 cut(s) 470, 1439, 1516, 1670, 1837
Tru1I TTAA 5 cut(s) 87, 162, 513, 1074, 1146
Tru9I TTAA 5 cut(s) 87, 162, 513, 1074, 1146
TscAI CASTG 3 cut(s) 132, 1200, 1297
TseFI GTSAC 4 cut(s) 907, 1088, 1160, 1195
TseI GCWGC 1 cut(s) 483
Tsp45I GTSAC 4 cut(s) 907, 1088, 1160, 1195
TspDTI ATGAA 7 cut(s) 114, 207, 452, 540, 885, 1023, 1628
TspGWI ACGGA 3 cut(s) 260, 388, 642
TspRI CASTG 3 cut(s) 132, 1200, 1297
VneI GTGCAC 2 cut(s) 1293, 1678
VpaK11BI GGWCC 2 cut(s) 361, 489
XapI RAATTY 2 cut(s) 1526, 1562
XceI RCATGY 1 cut(s) 1579
XmiI GTMKAC 1 cut(s) 745
XspI CTAG 3 cut(s) 753, 1086, 1320
Zsp2I ATGCAT 1 cut(s) 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.