pycom01g22660

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0015712
Physical Location & Seq
Reverse (-)
20167794 .. 20169569
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22660.1

Sequence Viewer

Length: 1662 bp
ATGGCGGGAACATGGAAGCACAACCGATGGAAGCCAATCCTTGAACAATTCTCAACCCTGCATTACAAAACTACGAAAACCCAAGACCCCAACATAGTTTCCACCAACATTTGCATAACTCGCCACTGCAAAAGCGGGCAGCTAGATGTTGCCCGGAAAATGTTCGACGAAATGCCAATCAGAACCGTCGTCTCATGGAACACCATGATTTCTGGATATTCAAAGTGGGGAAGATTCAGCGAGGCTCTACGGTTGGTCTCAGATATGCATCACGGCAATGTAAGGCTAAATGAGACGACTTTTTTGACAACTCTCAGCGCGTGCGCGCGTTCGGGGTCTTTGAGTGAGGGAAAGGAGGTTCATTGCTTGGTTTTGAAATCTGGGTTAGAGTGCTTTGAGCTTTTGGGTAGTTCTCTGTTGTACTTTTACGCTAGTTGCCTTAAAATTGAGGATGCTAAGCGGGTTTTCGATGAGCTGCGCGGTGGAAATGAGTTGTTGTGGAGCTTATTGCTTGTGGGCTATGTGCAGTGTAATTTGATGAGTGAAGCTACGGAGTTGTTTGTGAATATGCCGAAGCGGGATGTTTTGGCATGGACTACGATGATTTCGTGGTACGCTAAGAGCGAGGATGGCTGGAAGGCTGTGCATGGAATTTTGATCAAATATGGTTTTGAGTCTGATGAATCGATTGGTGGCGCATTACTAGAATTTTATTGTGTTTGTGGAGCTGTTCATTGTGCCAAGAGAGTTTATGATAGAATAGAAAGCCCGAGTTTAAATGCTTCGAATTCACTTATTGGAGGTCTTGTGTTGATGGATAGGCTTGAAGATGCAAAGCGAATTTTCAATAGACTAAAAGAAAAGGATTCAGTTTCGTATAACTTGATGATTAAAGGTTATGCAACGAGTGGCCAAGTCGATGAATCAGAGAGATTATTTGAGAGTATGCCTCATAGAACTATAATTTCTTCAAATACTATGATTTCTGTGCATTCTAGGAATGGTGATATCGATAAGGCTTCAAAGATTTTTGAAGAAACAAAAGAGGAAAGAAACCCTGTGACATGGAACGCCATGATGTCGGGTTACATTCAAAATCATCAGCATGAGGAGGCTTTGGAGTTATACGCAACAATGCACAAATTGTCAATAGACCGTACAAGATCCACTTTCTCTGTTCTGTTTCATGCCTGTTCATGCCTTGGATCCCTTCAATCGGGACAAGCACTTCATGGTCATTTGATTAAAACACCATTTGAATCAAATGTGTATGTTGGGACATCCCTGATAGATATGTACTCCAAATGCGGAAGCATCACCGATGCTGAAGCATCATTCATCTGCATTGGTTCACCCAATGTGGCAGCTTGGACAGCTCTCATTAACGGGTATGCGCAGTACGGGCTTGTCTCAAAGGCAATGTTGCTGTTTGAGAAAATGTTAAAGCAAGGAATCATTCCGAATGCTGCTACTTTTGTGGCAATTCTCTCTGCTTGCTGTCGTGCTGGTCTAGTCAACGAAGGGATGAGAATTTTCCACTTGATGGAAAAAAATTACGGAGTAAACCCTACACTTGAACACTATGCATGTGTAGTGGATCGTTCTCGGTCGGTCTGGTTGTTTACAAGAAGCTATGGAGTTTATTCAAGAAATGCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase E1-E2_ATPase PPR PPR_2 PhoLip_ATPase_C

Protein Analysis

554

Amino Acids

62.15

Weight (kDa)

7.82

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 62 - 110 3.7e-11 PPR repeat family
PPR PF01535 64 - 90 1e-07 PPR repeat
PPR_2 PF13041 288 - 320 6.6e-06 PPR repeat family
PPR PF01535 291 - 318 4e-06 PPR repeat
PPR_2 PF13041 352 - 399 3.4e-11 PPR repeat family
PPR PF01535 354 - 382 2.2e-06 PPR repeat
PPR_2 PF13041 452 - 501 1.5e-13 PPR repeat family
PPR_3 PF13812 452 - 499 1.6e-06 Pentatricopeptide repeat domain
PPR PF01535 456 - 485 3.2e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1395
AccB7I CCANNNNNTGG 1 cut(s) 1543
AccII CGCG 4 cut(s) 320, 326, 328, 480
AciI CCGC 6 cut(s) 5, 135, 460, 480, 577, 1308
AclWI GGATC 4 cut(s) 1158, 1200, 1213, 1605
AcoI YGGCCR 1 cut(s) 910
AcsI RAATTY 5 cut(s) 651, 707, 787, 840, 1530
AcuI CTGAAG 1 cut(s) 1347
AfaI GTAC 5 cut(s) 422, 614, 1159, 1298, 1400
AfiI CCNNNNNNNGG 2 cut(s) 1216, 1543
AloI GAACNNNNNNTCC 2 cut(s) 342, 374
AluBI AGCT 9 cut(s) 142, 400, 475, 504, 548, 728, 1367, 1376, 1632
AluI AGCT 9 cut(s) 142, 400, 475, 504, 548, 728, 1367, 1376, 1632
Alw26I GTCTC 4 cut(s) 196, 262, 287, 1414
AlwI GGATC 4 cut(s) 1158, 1200, 1213, 1605
Ama87I CYCGRG 1 cut(s) 769
AoxI GGCC 1 cut(s) 910
ApeKI GCWGC 4 cut(s) 139, 475, 1364, 1466
ApoI RAATTY 5 cut(s) 651, 707, 787, 840, 1530
ArsI GACNNNNNNTTYG 2 cut(s) 286, 318
Asp700I GAANNNNTTC 1 cut(s) 161
AspLEI GCGC 6 cut(s) 320, 326, 328, 480, 698, 1396
AsuC2I CCSGG 1 cut(s) 154
AsuHPI GGTGA 3 cut(s) 1016, 1309, 1344
AsuII TTCGAA 1 cut(s) 785
AvaI CYCGRG 1 cut(s) 769
BalI TGGCCA 1 cut(s) 912
BamHI GGATCC 1 cut(s) 1205
BbvI GCAGC 4 cut(s) 151, 462, 1376, 1453
BccI CCATC 4 cut(s) 21, 623, 808, 1537
BceAI ACGGC 1 cut(s) 289
BclI TGATCA 1 cut(s) 657
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 4 cut(s) 196, 262, 287, 1414
BfaI CTAG 5 cut(s) 143, 432, 704, 996, 1511
BisI GCNGC 4 cut(s) 140, 476, 1365, 1467
BlpI GCTNAGC 1 cut(s) 456
BlsI GCNGC 4 cut(s) 141, 477, 1366, 1468
Bme1390I CCNGG 1 cut(s) 154
BmeT110I CYCGRG 1 cut(s) 769
BmiI GGNNCC 1 cut(s) 1207
BmrFI CCNGG 1 cut(s) 154
BmsI GCATC 6 cut(s) 277, 442, 820, 1312, 1323, 1340
BplI GAGNNNNNCTC 2 cut(s) 934, 966
Bpu1102I GCTNAGC 1 cut(s) 456
Bpu14I TTCGAA 1 cut(s) 785
BpuMI CCSGG 1 cut(s) 154
Bsa29I ATCGAT 2 cut(s) 686, 1011
BsaBI GATNNNNATC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 262
BsaJI CCNNGG 1 cut(s) 1201
BsaXI ACNNNNNCTCC 2 cut(s) 792, 822
Bsc4I CCNNNNNNNGG 2 cut(s) 1216, 1543
Bse3DI GCAATG 3 cut(s) 283, 361, 1425
Bse8I GATNNNNATC 1 cut(s) 267
BseCI ATCGAT 2 cut(s) 686, 1011
BseDI CCNNGG 1 cut(s) 1201
BseGI GGATG 5 cut(s) 457, 586, 634, 1280, 1530
BseJI GATNNNNATC 1 cut(s) 267
BseLI CCNNNNNNNGG 2 cut(s) 1216, 1543
BseMI GCAATG 3 cut(s) 283, 361, 1425
BseMII CTCAG 2 cut(s) 273, 328
BsePI GCGCGC 1 cut(s) 324
BseRI GAGGAG 1 cut(s) 1124
BseXI GCAGC 4 cut(s) 151, 462, 1376, 1453
BsgI GTGCAG 1 cut(s) 545
Bsh1236I CGCG 4 cut(s) 320, 326, 328, 480
Bsh1285I CGRYCG 1 cut(s) 1610
BshFI GGCC 1 cut(s) 912
BshVI ATCGAT 2 cut(s) 686, 1011
BsiEI CGRYCG 1 cut(s) 1610
BsiHKCI CYCGRG 1 cut(s) 769
BsiSI CCGG 1 cut(s) 154
BslFI GGGAC 2 cut(s) 1233, 1291
BslI CCNNNNNNNGG 2 cut(s) 1216, 1543
BsmAI GTCTC 4 cut(s) 196, 262, 287, 1414
BsmBI CGTCTC 2 cut(s) 196, 287
BsmFI GGGAC 2 cut(s) 1233, 1291
BsmI GAATGC 2 cut(s) 991, 1468
BsnI GGCC 1 cut(s) 912
Bso31I GGTCTC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 769
Bsp119I TTCGAA 1 cut(s) 785
Bsp143I GATC 4 cut(s) 657, 1163, 1205, 1597
Bsp1720I GCTNAGC 1 cut(s) 456
BspACI CCGC 6 cut(s) 5, 135, 460, 480, 577, 1308
BspANI GGCC 1 cut(s) 912
BspCNI CTCAG 2 cut(s) 272, 327
BspDI ATCGAT 2 cut(s) 686, 1011
BspFNI CGCG 4 cut(s) 320, 326, 328, 480
BspLI GGNNCC 1 cut(s) 1207
BspPI GGATC 4 cut(s) 1158, 1200, 1213, 1605
BspT104I TTCGAA 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 262
BsrDI GCAATG 3 cut(s) 283, 361, 1425
BssECI CCNNGG 1 cut(s) 1201
BssHII GCGCGC 1 cut(s) 324
BssMI GATC 4 cut(s) 657, 1163, 1205, 1597
BssT1I CCWWGG 1 cut(s) 1201
Bst4CI ACNGT 3 cut(s) 187, 252, 1157
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 4 cut(s) 137, 322, 326, 1495
BstDEI CTNAG 4 cut(s) 259, 314, 456, 618
BstF5I GGATG 5 cut(s) 457, 586, 634, 1280, 1530
BstFNI CGCG 4 cut(s) 320, 326, 328, 480
BstHHI GCGC 6 cut(s) 320, 326, 328, 480, 698, 1396
BstKTI GATC 4 cut(s) 660, 1166, 1208, 1600
BstMAI GTCTC 4 cut(s) 196, 262, 287, 1414
BstMBI GATC 4 cut(s) 657, 1163, 1205, 1597
BstMCI CGRYCG 1 cut(s) 1610
BstMWI GCNNNNNNNGC 4 cut(s) 120, 630, 1373, 1402
BstNSI RCATGY 1 cut(s) 1590
BstSCI CCNGG 1 cut(s) 152
BstUI CGCG 4 cut(s) 320, 326, 328, 480
BstV1I GCAGC 4 cut(s) 151, 462, 1376, 1453
BstX2I RGATCY 2 cut(s) 1163, 1205
BstYI RGATCY 2 cut(s) 1163, 1205
Bsu15I ATCGAT 2 cut(s) 686, 1011
BsuRI GGCC 1 cut(s) 912
BsuTUI ATCGAT 2 cut(s) 686, 1011
BtsCI GGATG 5 cut(s) 457, 586, 634, 1280, 1530
BtsI GCAGTG 2 cut(s) 124, 533
BtsIMutI CAGTG 2 cut(s) 124, 533
Cac8I GCNNGC 4 cut(s) 137, 322, 326, 1495
CfoI GCGC 6 cut(s) 320, 326, 328, 480, 698, 1396
ClaI ATCGAT 2 cut(s) 686, 1011
Csp6I GTAC 5 cut(s) 421, 613, 1158, 1297, 1399
CviQI GTAC 5 cut(s) 421, 613, 1158, 1297, 1399
DdeI CTNAG 4 cut(s) 259, 314, 456, 618
DpnI GATC 4 cut(s) 659, 1165, 1207, 1599
DpnII GATC 4 cut(s) 657, 1163, 1205, 1597
DraI TTTAAA 1 cut(s) 777
EaeI YGGCCR 1 cut(s) 910
Eco130I CCWWGG 1 cut(s) 1201
Eco31I GGTCTC 1 cut(s) 262
Eco32I GATATC 1 cut(s) 1009
Eco57I CTGAAG 1 cut(s) 1347
Eco88I CYCGRG 1 cut(s) 769
EcoRI GAATTC 1 cut(s) 787
EcoRV GATATC 1 cut(s) 1009
EcoT14I CCWWGG 1 cut(s) 1201
EcoT22I ATGCAT 2 cut(s) 270, 1588
ErhI CCWWGG 1 cut(s) 1201
Esp3I CGTCTC 2 cut(s) 196, 287
FalI AAGNNNNNCTT 2 cut(s) 1153, 1185
FaqI GGGAC 2 cut(s) 1233, 1291
FauI CCCGC 3 cut(s) 128, 453, 570
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 4 cut(s) 140, 476, 1365, 1467
FokI GGATG 5 cut(s) 464, 593, 641, 1267, 1537
Fsp4HI GCNGC 4 cut(s) 140, 476, 1365, 1467
FspBI CTAG 5 cut(s) 143, 432, 704, 996, 1511
FspI TGCGCA 1 cut(s) 1395
GlaI GCGC 6 cut(s) 319, 325, 327, 479, 697, 1395
GluI GCNGC 4 cut(s) 140, 476, 1365, 1467
HaeIII GGCC 1 cut(s) 912
HapII CCGG 1 cut(s) 154
HhaI GCGC 6 cut(s) 320, 326, 328, 480, 698, 1396
Hin6I GCGC 6 cut(s) 318, 324, 326, 478, 696, 1394
HinP1I GCGC 6 cut(s) 318, 324, 326, 478, 696, 1394
HincII GTYRAC 1 cut(s) 1516
HindII GTYRAC 1 cut(s) 1516
HinfI GANTC 7 cut(s) 234, 674, 683, 866, 923, 1259, 1452
HpaII CCGG 1 cut(s) 154
HphI GGTGA 3 cut(s) 1016, 1309, 1344
Hpy166II GTNNAC 4 cut(s) 1352, 1516, 1564, 1623
Hpy188I TCNGA 5 cut(s) 182, 262, 679, 928, 1461
Hpy188III TCNNGA 3 cut(s) 213, 1218, 1647
Hpy8I GTNNAC 4 cut(s) 1352, 1516, 1564, 1623
Hpy99I CGWCG 2 cut(s) 170, 191
HpyAV CCTTC 3 cut(s) 631, 1220, 1514
HpyCH4III ACNGT 3 cut(s) 187, 252, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 630, 1373, 1402
HpyF3I CTNAG 4 cut(s) 259, 314, 456, 618
HspAI GCGC 6 cut(s) 318, 324, 326, 478, 696, 1394
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 4 cut(s) 657, 1163, 1205, 1597
LmnI GCTCC 2 cut(s) 501, 725
Lsp1109I GCAGC 4 cut(s) 151, 462, 1376, 1453
LweI GCATC 6 cut(s) 277, 442, 820, 1312, 1323, 1340
MaeI CTAG 5 cut(s) 143, 432, 704, 996, 1511
MaeIII GTNAC 2 cut(s) 1060, 1085
MalI GATC 4 cut(s) 659, 1165, 1207, 1599
MboI GATC 4 cut(s) 657, 1163, 1205, 1597
MboII GAAGA 4 cut(s) 243, 839, 960, 1046
MfeI CAATTG 1 cut(s) 1657
MflI RGATCY 2 cut(s) 1163, 1205
MlsI TGGCCA 1 cut(s) 912
MluNI TGGCCA 1 cut(s) 912
MlyI GAGTC 1 cut(s) 683
Mox20I TGGCCA 1 cut(s) 912
Mph1103I ATGCAT 2 cut(s) 270, 1588
MroXI GAANNNNTTC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 912
MseI TTAA 6 cut(s) 441, 776, 891, 1245, 1383, 1442
MslI CAYNNNNRTG 2 cut(s) 276, 1104
Msp20I TGGCCA 1 cut(s) 912
MspI CCGG 1 cut(s) 154
MspR9I CCNGG 1 cut(s) 154
MunI CAATTG 1 cut(s) 1657
Mva1269I GAATGC 2 cut(s) 991, 1468
MvnI CGCG 4 cut(s) 320, 326, 328, 480
MwoI GCNNNNNNNGC 4 cut(s) 120, 630, 1373, 1402
NciI CCSGG 1 cut(s) 154
NdeII GATC 4 cut(s) 657, 1163, 1205, 1597
NlaIV GGNNCC 1 cut(s) 1207
NmuCI GTSAC 1 cut(s) 1060
NsbI TGCGCA 1 cut(s) 1395
NsiI ATGCAT 2 cut(s) 270, 1588
NspI RCATGY 1 cut(s) 1590
NspV TTCGAA 1 cut(s) 785
PauI GCGCGC 1 cut(s) 324
PcsI WCGNNNNNNNCGW 2 cut(s) 605, 621
PctI GAATGC 2 cut(s) 991, 1468
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 6 cut(s) 234, 683, 866, 923, 1259, 1452
PflMI CCANNNNNTGG 1 cut(s) 1543
PkrI GCNGC 4 cut(s) 141, 477, 1366, 1468
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
PspN4I GGNNCC 1 cut(s) 1207
PsuI RGATCY 2 cut(s) 1163, 1205
PteI GCGCGC 1 cut(s) 324
RsaI GTAC 5 cut(s) 422, 614, 1159, 1298, 1400
RsaNI GTAC 5 cut(s) 421, 613, 1158, 1297, 1399
RseI CAYNNNNRTG 2 cut(s) 276, 1104
SaqAI TTAA 6 cut(s) 441, 776, 891, 1245, 1383, 1442
SatI GCNGC 4 cut(s) 140, 476, 1365, 1467
Sau3AI GATC 4 cut(s) 657, 1163, 1205, 1597
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 154
SfaNI GCATC 6 cut(s) 277, 442, 820, 1312, 1323, 1340
SfuI TTCGAA 1 cut(s) 785
SmiMI CAYNNNNRTG 2 cut(s) 276, 1104
SsiI CCGC 6 cut(s) 5, 135, 460, 480, 577, 1308
SspMI CTAG 5 cut(s) 143, 432, 704, 996, 1511
StyD4I CCNGG 1 cut(s) 152
StyI CCWWGG 1 cut(s) 1201
TaaI ACNGT 3 cut(s) 187, 252, 1157
TaqI TCGA 6 cut(s) 165, 468, 686, 785, 918, 1011
TaqII GACCGA 2 cut(s) 1596, 1600
TatI WGTACW 2 cut(s) 420, 1296
TfiI GAWTC 6 cut(s) 234, 683, 866, 923, 1259, 1452
Tru1I TTAA 6 cut(s) 441, 776, 891, 1245, 1383, 1442
Tru9I TTAA 6 cut(s) 441, 776, 891, 1245, 1383, 1442
TscAI CASTG 2 cut(s) 131, 533
TseFI GTSAC 1 cut(s) 1060
TseI GCWGC 4 cut(s) 139, 475, 1364, 1466
Tsp45I GTSAC 1 cut(s) 1060
TspDTI ATGAA 8 cut(s) 350, 696, 722, 936, 1175, 1185, 1220, 1327
TspGWI ACGGA 2 cut(s) 566, 1572
TspRI CASTG 2 cut(s) 131, 533
Van91I CCANNNNNTGG 1 cut(s) 1543
XapI RAATTY 5 cut(s) 651, 707, 787, 840, 1530
XceI RCATGY 1 cut(s) 1590
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 5 cut(s) 143, 432, 704, 996, 1511
Zsp2I ATGCAT 2 cut(s) 270, 1588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.