pycom01g23520

Auxin-induced protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011864
Physical Location & Seq
Forward (+)
20823317 .. 20823915
599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 453 bp
ATGAGGTTTTCTGATTTTCGGGTTTTTCGAGTTTTCGAAAAGATGGGATTTATCGCCGCCGACAGGCCCAGCAAGCACAACCACCTGAACTTCCACCTCCACTTCCCCCACCTTCTCCACCACCACCACGACAAGAACAGCAAGGAGCTCAAAGATATTCCGAAAGGCTGTATGGCGGTGATGGTGGGCCAGGGGGAGGACCAGCAGAGGTTTGTTATTCCGGTGGTCTACATCAACCACCCGCTCTTCATGCAGCTGCTGAAGGAGGCGGAGAAGGAGTACGGATTCGATCACAAGGGTCCGATCACTATTCCTTGCCACGTGGAGGAGTTTCGCACCGTTCAAGGCATGATCGATAGGGAGACTTCGTTCCACGGCCATCATCATCATCACCACCACCATCACCATCACAACCACCACCATCACCACTATGCCTGGTGTTTTAGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

151

Amino Acids

18.09

Weight (kDa)

7.99

Isoelectric Point (pI)

25.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 43 - 117 1.2e-22 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 228
AciI CCGC 4 cut(s) 57, 176, 242, 269
AcoI YGGCCR 1 cut(s) 376
AcuI CTGAAG 1 cut(s) 281
AcvI CACGTG 1 cut(s) 322
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 3 cut(s) 63, 196, 325
AgsI TTSAA 1 cut(s) 344
AjnI CCWGG 2 cut(s) 189, 434
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 2 cut(s) 148, 256
AluI AGCT 2 cut(s) 148, 256
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 356
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 3 cut(s) 65, 187, 376
ApeKI GCWGC 2 cut(s) 253, 256
AspS9I GGNCC 4 cut(s) 66, 187, 199, 299
AsuHPI GGTGA 4 cut(s) 190, 383, 395, 416
AsuII TTCGAA 1 cut(s) 36
AvaII GGWCC 2 cut(s) 199, 299
BanII GRGCYC 1 cut(s) 150
BbrPI CACGTG 1 cut(s) 322
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 243, 265
BccI CCATC 6 cut(s) 37, 175, 387, 408, 414, 429
BceAI ACGGC 1 cut(s) 391
BciT130I CCWGG 2 cut(s) 191, 436
BcoDI GTCTC 1 cut(s) 356
BisI GCNGC 3 cut(s) 57, 254, 257
BlsI GCNGC 3 cut(s) 58, 255, 258
Bme1390I CCNGG 2 cut(s) 191, 436
Bme18I GGWCC 2 cut(s) 199, 299
BmgT120I GGNCC 4 cut(s) 66, 187, 199, 299
BmiI GGNNCC 1 cut(s) 300
BmrFI CCNGG 2 cut(s) 191, 436
Bpu14I TTCGAA 1 cut(s) 36
Bsa29I ATCGAT 1 cut(s) 354
BsaAI YACGTR 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 190, 373
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 353, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 196, 325
BseBI CCWGG 2 cut(s) 191, 436
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 190, 373
BseLI CCNNNNNNNGG 3 cut(s) 63, 196, 325
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 2 cut(s) 243, 265
BseYI CCCAGC 1 cut(s) 68
BshFI GGCC 3 cut(s) 67, 189, 378
BshVI ATCGAT 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 1 cut(s) 221
BslI CCNNNNNNNGG 3 cut(s) 63, 196, 325
BsmAI GTCTC 1 cut(s) 356
BsnI GGCC 3 cut(s) 67, 189, 378
Bsp119I TTCGAA 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 3 cut(s) 289, 303, 351
BspACI CCGC 4 cut(s) 57, 176, 242, 269
BspANI GGCC 3 cut(s) 67, 189, 378
BspDI ATCGAT 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 300
BspQI GCTCTTC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 244
BssECI CCNNGG 2 cut(s) 190, 373
BssMI GATC 3 cut(s) 289, 303, 351
Bst2UI CCWGG 2 cut(s) 191, 436
Bst4CI ACNGT 1 cut(s) 340
Bst6I CTCTTC 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 74
BstDSI CCRYGG 1 cut(s) 373
BstKTI GATC 3 cut(s) 292, 306, 354
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 3 cut(s) 289, 303, 351
BstMWI GCNNNNNNNGC 2 cut(s) 73, 250
BstNI CCWGG 2 cut(s) 191, 436
BstSCI CCNGG 2 cut(s) 189, 434
BstV1I GCAGC 2 cut(s) 243, 265
Bsu15I ATCGAT 1 cut(s) 354
BsuRI GGCC 3 cut(s) 67, 189, 378
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 373
Cac8I GCNNGC 1 cut(s) 74
CaiI CAGNNNCTG 1 cut(s) 259
Cfr13I GGNCC 4 cut(s) 66, 187, 199, 299
ClaI ATCGAT 1 cut(s) 354
Csp6I GTAC 1 cut(s) 280
CviAII CATG 2 cut(s) 250, 349
CviJI RGCY 6 cut(s) 67, 148, 168, 189, 256, 378
CviKI_1 RGCY 6 cut(s) 67, 148, 168, 189, 256, 378
CviQI GTAC 1 cut(s) 280
DpnI GATC 3 cut(s) 291, 305, 353
DpnII GATC 3 cut(s) 289, 303, 351
EaeI YGGCCR 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 284
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 199, 299
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 281
Eco72I CACGTG 1 cut(s) 322
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 189, 434
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 2 cut(s) 253, 352
FaiI YATR 4 cut(s) 173, 251, 350, 432
FatI CATG 2 cut(s) 249, 348
FauI CCCGC 1 cut(s) 249
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 57, 254, 257
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 3 cut(s) 57, 254, 257
GluI GCNGC 3 cut(s) 57, 254, 257
GsaI CCCAGC 1 cut(s) 72
HaeIII GGCC 3 cut(s) 67, 189, 378
HapII CCGG 1 cut(s) 221
Hin1II CATG 2 cut(s) 253, 352
HinfI GANTC 1 cut(s) 285
HpaII CCGG 1 cut(s) 221
HphI GGTGA 4 cut(s) 190, 383, 395, 416
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 13, 162, 303
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 122, 256, 268
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 1 cut(s) 253
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 250
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 2 cut(s) 253, 352
Kzo9I GATC 3 cut(s) 289, 303, 351
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 1 cut(s) 145
LpnPI CCDG 9 cut(s) 49, 82, 98, 176, 203, 215, 234, 421, 448
Lsp1109I GCAGC 2 cut(s) 243, 265
MaeII ACGT 1 cut(s) 321
MalI GATC 3 cut(s) 291, 305, 353
MbiI CCGCTC 1 cut(s) 244
MboI GATC 3 cut(s) 289, 303, 351
MboII GAAGA 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 150
MnlI CCTC 5 cut(s) 107, 190, 201, 259, 319
MslI CAYNNNNRTG 1 cut(s) 429
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 191, 436
MvaI CCWGG 2 cut(s) 191, 436
MwoI GCNNNNNNNGC 2 cut(s) 73, 250
NdeII GATC 3 cut(s) 289, 303, 351
NlaIII CATG 2 cut(s) 253, 352
NlaIV GGNNCC 1 cut(s) 300
NspV TTCGAA 1 cut(s) 36
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 3 cut(s) 58, 255, 258
PmaCI CACGTG 1 cut(s) 322
PmlI CACGTG 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 189, 434
PspCI CACGTG 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 68
PspGI CCWGG 2 cut(s) 189, 434
PspN4I GGNNCC 1 cut(s) 300
PspPI GGNCC 4 cut(s) 66, 187, 199, 299
PstNI CAGNNNCTG 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 256
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 429
SacI GAGCTC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 251
SatI GCNGC 3 cut(s) 57, 254, 257
Sau3AI GATC 3 cut(s) 289, 303, 351
Sau96I GGNCC 4 cut(s) 66, 187, 199, 299
ScrFI CCNGG 2 cut(s) 191, 436
SduI GDGCHC 1 cut(s) 150
SetI ASST 8 cut(s) 8, 87, 99, 114, 150, 212, 258, 324
SfuI TTCGAA 1 cut(s) 36
SinI GGWCC 2 cut(s) 199, 299
SmiMI CAYNNNNRTG 1 cut(s) 429
SsiI CCGC 4 cut(s) 57, 176, 242, 269
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 2 cut(s) 189, 434
TaaI ACNGT 1 cut(s) 340
TaiI ACGT 1 cut(s) 324
TaqI TCGA 4 cut(s) 28, 36, 288, 354
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 285
TseI GCWGC 2 cut(s) 253, 256
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 1 cut(s) 297
VpaK11BI GGWCC 2 cut(s) 199, 299
XmiI GTMKAC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.