Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0010104

Physical Location & Seq

Genomic Coordinates Forward (+)

21367975 .. 21374067

Gene Structure 6093 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2850 bp

ATGACAACAAGAGGGGAAGTAATGCTCCATTTCACTTCCATTGTTTTTCATCCTTCATTCTCCAAACTCCAGCCCCAGCCACAAATGGATTATCATCTTAACAATATTTTACAATTCACCAGCCCAACTGAAGCCCACCACCTCCTCGGCCCATATTTTGGGCTGCCTCCGTCTTTCTTTTCGTTAAACCCTATTTTGTTTCTGTTGCGGAGCTCCACCAATCCTCTCATGGCGTCTCTACCTTTGAAGAAGAACTACCGGTGCGTGCCCTCTCTGCAACAGTTCTACTCCGGCGGCCCCTTCGCCGTCTCATCCGACGGCTCCTTCATCGCCTGCAAATGCGGCGAGTCCATTAAGATCGTGGATTCATTGGACGCCTCGATTCGTTCCACTATTGAAGCCGACTTAGACGAAGTTACCGCTTTGGCCCTCAGCCCCGACGACAGTCTGCTCTTCTCTGCCGGTCACAGTCGCCAAATTAGGATTTGGGACCTCTCCACCCTCAAGTGCATGCGTTCTTGGAAGGGTCATGAGGGGCCAGTGATGGGAATGGCTTGTCACCCGTCTGGAGGATTGCTTGCGACTGCCGGAGCTGATAGGAAGGTTCTTGTTTGGGATGTTGATGGCATCTTTCGCACTCATTACTTTAAAGGCCACAAAGGGGTTGTTTCTAGTGTTCTCTTCCATCCTGATCCTACTAAACAACTTCTTTTCTCCGCAAGCGATGATACAACTGTGCATGTTTGGGACTTGTTAACGAAGATGTGCGTTGCAACGCTTAATGGGCATAACTCCACAGTAACTTCTATGGCACTCTCTGAAGACGGATGGACATTGCTCACAGCTGGAAGAGATAAGGTTGTAATTTTGTGGGACCTCCATGACTATAGCTGCATGAAGACTGTAACAACATATGAGGTTCTTGAAGCTGTGTGTGCAATCTATTCTGGAACTCCATTGTCATCCTATTTGGGTTCCTGCAAGAAGCAGAATGGGAAGAAAAGTGGATTAAGTGAAACTTGTTTTATAACAGTTGGTGACCGTGGGATTGTACGGATTTGGAATTCTGAAGGTGCAGTTTGCCTATATGAGCAGAAGTCCTCAGATGTCACTCCATCTTCAGATGTGGATGAATCAAACAGGGGATTCACCGCAGCCGCCATGCTTCCTTCAGATAAAGGGTTGCTTTGTGTGACTGCTGATCAGGAGTTCCTTTTATATTCCCCAGTTAAGTTGCCAGAGGGGACGTTGGAATTTGAATTGAGCAAAAGACTTGTAGGTTACCACGATGAGATTGTGGATATGAAGTTTGTAGGAGATGAGGAACAATTCCTTGCTGTTGCCACAAATCTTGAACAGGTACGAGTATATGACATTGCGTCTATGTCATGTTCTTATGTATTGGCAGGTCATACCGGAATTGTTCTATGCCTTGACACCTGTGTATTGAGTTGTGGAAAACCACTTATTGTTACTGGGAGCAAAGACAACACTGTTCGGTTGTGGGATTCAGAAAGCAAATGTTGCCTTGGTCTTGGCATAGGTCATATGGGAGGTATTGGAGCTATTGCTTTCTCAAAAAAGCGGAAAGATTTCTTCGTTAGTGGCAGTAGTGATCGTACCATCAAGGTGTGGAGTTTGGATGGTCTTTCAGACAATACAGAAAAGCCAGTGAATCTGAAAGCAAAGGCTGGTGTAGCAGCCCACGATAAGGATATCAATTCAGTAGCTGTTGCACCAAATGATAGTTTAGTTTGTAGTGGTTCACAGGATCGCACTGCTTGTGTATGGAGGCTTCCGGATCTTGTACCAGTAGTTGTACTTAAGGGTCATAAAAGGGGGGTTTGGTCTGTAGAGTTCTCTCCCGTTGATCAATGTGTTATAACAGCTTCTGGTGATAAAACAATAAAGATATGGGCCATATCTGATGGTTCGTGCTTGAAAACATTTGAAGGGCATACGTCAAGCGTATTAAGAGCATCATTTCTTACTCGTGGAACCCAATTTATTTCTTCAGGTGCTGATGGGTTGGTAAAACTGTGGATGGTCAAAACCGATGAATGCATTGCTACATATGATCAGCATGACAACAAGGTTTATGCCTTGGCTGTTGGAAAAAAGACAGAAATGCTTGCAACTGGCAGCAGCGATGCTGTCATCAATATGTGGTATGATTGTACTGCTTCTGATAAAGAGGAAGCTTTTCGTAGAGAGGAGGAAGGTGTTTTAAAGAATCAAGAGCTAGAAAATGCTGTATTAGATGCCGACTTTTCTAAAGCAATCCAAGTCGCATTTGAGCTTCGCAGGCCTCATAAGCTTTATCAGTGCTTTTCTGAAATTTGGAGGAAGAGAGAGACTGAAAAGCAGATGGAGAAAGCCCTTCTTGCCCTCGGCGAGGAAGAGATCGATCTACTATTTGAATATGTTCGAGAATGGAATACAAAGCCAAAGCTCTGTCATGTTGCTCACTTTGTGCTTTCAAAAGTTTTCAACATCCTCAGTCCAACAAAGATTAACGAGATAAAAGGCATTGGGGAAATCCTTGAAGGTCTCCTCTCGTATTCTCAGAGGCATTTTAGCCGAATGGACAGGCACGAAACAAACACGTATTTGGTGAACTACACTCTGACTGGAATGTCAGTCATTGAACCAGAGACAGATACGCGAGTATTGCATGATAAGTCTCTGATGCACTCCTCTGCGGACGATGAGAAAGGGACTTTAATAAACGAACCTGAAGATGAGGAGCAAAAAACCTCTCAACGTTTGGGAGAAGAGGTAGCTACGAAGAAACGGAAGTCAAAGAAATCAAAAGATGGTTCCACTTGCAATAAGAAAGTAAAGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000462 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030515 GO:0030684 GO:0030686 GO:0031974 GO:0031981 GO:0032040 GO:0032991 GO:0034388 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Utp13 WD40

Protein Analysis

950

Amino Acids

104.88

Weight (kDa)

6.04

Isoelectric Point (pI)

39.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	101 - 231	2.8e-18	THOC3 beta-propeller domain
Beta-prop_DCAF4	PF23761	102 - 207	4.8e-06	DDB1- and CUL4-associated factor 4 beta-propeller domain
WD40_WDHD1_1st	PF24817	102 - 176	2.3e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	103 - 232	6.4e-20	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	105 - 260	6.4e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	109 - 293	2.4e-36	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	116 - 319	4.9e-17	G protein beta WD-40 repeat protein
WD40_Prp19	PF24814	117 - 312	1.3e-34	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	132 - 311	3.9e-18	TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	137 - 289	3.6e-16	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML	PF23409	139 - 325	1.2e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2	PF23414	140 - 278	5.6e-15	Echinoderm microtubule-associated protein second beta-propeller
WD40_MABP1-WDR62_2nd	PF24782	142 - 251	2e-09	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	167 - 292	8.3e-18	WDR3 second beta-propeller domain
WD40	PF00400	169 - 206	3.3e-08	WD domain, G-beta repeat
WDR55	PF24796	172 - 300	7.4e-10	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	175 - 301	5.7e-14	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	180 - 261	9.7e-10	WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	184 - 293	1.5e-11	MABP1/WDR62 second WD40 domain
Beta-prop_WDR5	PF25175	187 - 315	1.7e-25	WDR5 beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	188 - 297	2.4e-10	WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_1st	PF24780	197 - 300	5.3e-07	MABP1/WDR62 first WD40 domain
WD40	PF00400	214 - 250	1.3e-06	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	215 - 304	2.6e-20	WDR3 first beta-propeller domain
EIF3I	PF24805	216 - 328	2e-08	EIF3I
Beta-prop_THOC3	PF25174	232 - 306	1.6e-13	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	233 - 308	1.4e-09	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	234 - 303	1.2e-07	CDC20/Fizzy WD40 domain
WD40	PF00400	256 - 292	8.8e-06	WD domain, G-beta repeat
Beta-prop_WDR3_2nd	PF25172	340 - 598	4.4e-19	WDR3 second beta-propeller domain
WD40_WDHD1_1st	PF24817	341 - 509	6.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	381 - 547	4.3e-19	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	387 - 547	2.9e-08	G protein beta WD-40 repeat protein
Beta-prop_THOC3	PF25174	426 - 548	4.2e-15	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	427 - 548	7.3e-17	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	429 - 550	3.2e-10	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	441 - 682	4.1e-19	MABP1/WDR62 second WD40 domain
WD40	PF00400	463 - 503	4.8e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	476 - 549	4.4e-08	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	513 - 687	2.3e-14	TEP-1 second beta-propeller
Beta-prop_WDR3_2nd	PF25172	516 - 724	1.3e-31	WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	521 - 650	1.2e-09	CAF1B/HIR1 beta-propeller domain
Beta-prop_EML_2	PF23414	528 - 666	1.1e-11	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	529 - 650	1.5e-06	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	561 - 723	2.3e-20	CDC20/Fizzy WD40 domain
WD40_Prp19	PF24814	562 - 723	2.1e-25	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	566 - 723	5.8e-33	WDR3 first beta-propeller domain
Beta-prop_SCAP	PF24017	567 - 682	6.2e-10	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	567 - 726	8.4e-09	WDR36/Utp21 second beta-propeller domain
EIF3I	PF24805	567 - 668	2.4e-11	EIF3I
Beta-prop_WDR5	PF25175	567 - 725	2.1e-36	WDR5 beta-propeller domain
WD40	PF00400	568 - 597	7.1e-06	WD domain, G-beta repeat
Beta-prop_EML	PF23409	568 - 664	5.6e-09	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	569 - 652	1.5e-14	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	570 - 650	1.1e-11	WDHD1 first WD40 domain
WDR55	PF24796	570 - 723	8.8e-12	WDR55
WD40	PF00400	604 - 639	2e-08	WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd	PF24782	614 - 723	3.1e-07	MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	623 - 753	1.2e-07	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	624 - 728	4e-14	G protein beta WD-40 repeat protein
Beta-prop_SCAP	PF24017	627 - 727	1.3e-06	SCAP Beta-propeller
Beta-prop_THOC3	PF25174	643 - 724	2.8e-12	THOC3 beta-propeller domain
WD40	PF00400	644 - 681	1.9e-06	WD domain, G-beta repeat
EIF3I	PF24805	650 - 724	1.1e-07	EIF3I
Utp13	PF08625	745 - 879	6.4e-39	Utp13 specific WD40 associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

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Orthologous Genes (Group: OG0010104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16750
fragaria_vesca	FvH4_7g34000 FvH4_7g34000
malus_domestica	MD01G1236400.v1.1 MD07G1309100.v1.1
prunus_persica	Prupe.2G326400_v2.0.a1 Prupe.2G326400_v2.0.a1
pyrus_communis	pycom01g24430 pycom07g28110
rosa_chinensis	RchiOBHm_Chr1g0384251
rosa_laevigata	RLG00000026054
rosa_multiflora	Rmu_sc0011271.1_g000002
rosa_roxburghii	Rroxscaffold_4G00276810
rosa_rugosa	Rorug01G0446100 Rorug01G0446200 Rorug01G0446200
rosa_samantha	Rh1AG471700 Rh1BG429400 Rh1CG442000 Rh1DG462100
rosa_wichuraiana	Rw1G040090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1028, 1883
AasI	GACNNNNNNGTC	1 cut(s)	2635
Acc36I	ACCTGC	1 cut(s)	1397
AccB7I	CCANNNNNTGG	1 cut(s)	158
AccII	CGCG	1 cut(s)	2665
AccIII	TCCGGA	1 cut(s)	1798
AciI	CCGC	9 cut(s)	208, 294, 342, 420, 717, 1152, 1158, 1585, 2702
AclI	AACGTT	1 cut(s)	2764
AclWI	GGATC	3 cut(s)	686, 1779, 1809
AcsI	RAATTY	3 cut(s)	1063, 1253, 2337
AcuI	CTGAAG	7 cut(s)	150, 840, 1089, 1104, 1155, 1998, 2757
AcyI	GRCGYC	2 cut(s)	233, 375
AdeI	CACNNNGTG	1 cut(s)	2473
AfaI	GTAC	6 cut(s)	1053, 1362, 1621, 1809, 1821, 2179
AfiI	CCNNNNNNNGG	6 cut(s)	158, 545, 569, 661, 1711, 2395
AflII	CTTAAG	1 cut(s)	1823
AflIII	ACRYGT	1 cut(s)	2604
AgeI	ACCGGT	1 cut(s)	258
AhdI	GACNNNNNGTC	1 cut(s)	1378
Alw21I	GWGCWC	1 cut(s)	215
Alw26I	GTCTC	6 cut(s)	240, 313, 2348, 2555, 2648, 2688
AlwI	GGATC	3 cut(s)	686, 1779, 1809
AlwNI	CAGNNNCTG	3 cut(s)	1730, 1892, 2021
Aor13HI	TCCGGA	1 cut(s)	1798
AoxI	GGCC	7 cut(s)	148, 295, 426, 536, 652, 1917, 2306
ApeKI	GCWGC	6 cut(s)	163, 891, 1154, 1700, 2142, 2145
ApoI	RAATTY	3 cut(s)	1063, 1253, 2337
AsiGI	ACCGGT	1 cut(s)	258
Asp700I	GAANNNNTTC	3 cut(s)	1592, 2202, 2424
AspS9I	GGNCC	7 cut(s)	149, 296, 427, 490, 536, 874, 1917
AsuHPI	GGTGA	6 cut(s)	109, 551, 1049, 1141, 1907, 2626
AvaII	GGWCC	2 cut(s)	490, 874
BaeGI	GKGCMC	1 cut(s)	270
BaeI	ACNNNNGTAYC	4 cut(s)	720, 753, 2652, 2685
BanII	GRGCYC	1 cut(s)	215
BarI	GAAGNNNNNNTAC	4 cut(s)	239, 271, 1779, 1811
BauI	CACGAG	1 cut(s)	1992
BbsI	GAAGAC	2 cut(s)	828, 905
Bbv12I	GWGCWC	1 cut(s)	215
BbvCI	CCTCAGC	1 cut(s)	431
BbvI	GCAGC	6 cut(s)	150, 878, 1166, 1712, 2154, 2157
BceAI	ACGGC	2 cut(s)	290, 334
BclI	TGATCA	3 cut(s)	1201, 1870, 2077
BcoDI	GTCTC	6 cut(s)	240, 313, 2348, 2555, 2648, 2688
BfaI	CTAG	2 cut(s)	672, 2243
BfmI	CTRYAG	2 cut(s)	886, 1851
BfrI	CTTAAG	1 cut(s)	1823
BfuAI	ACCTGC	1 cut(s)	1397
BisI	GCNGC	9 cut(s)	164, 295, 343, 892, 1155, 1158, 1701, 2143, 2146
BlsI	GCNGC	9 cut(s)	165, 296, 344, 893, 1156, 1159, 1702, 2144, 2147
Bme18I	GGWCC	2 cut(s)	490, 874
BmeRI	GACNNNNNGTC	1 cut(s)	1378
BmgT120I	GGNCC	7 cut(s)	149, 296, 427, 490, 536, 874, 1917
BmiI	GGNNCC	8 cut(s)	298, 322, 491, 537, 875, 976, 1999, 2821
BmrI	ACTGGG	2 cut(s)	1220, 1485
BmsI	GCATC	5 cut(s)	636, 1988, 2140, 2251, 2679
BmuI	ACTGGG	2 cut(s)	1220, 1485
BoxI	GACNNNNGTC	1 cut(s)	444
BpiI	GAAGAC	2 cut(s)	828, 905
BpmI	CTGGAG	2 cut(s)	53, 588
Bpu10I	CCTNAGC	1 cut(s)	431
BpuEI	CTTGAG	1 cut(s)	488
Bsa29I	ATCGAT	1 cut(s)	2406
BsaAI	YACGTR	1 cut(s)	2607
BsaBI	GATNNNNATC	1 cut(s)	93
BsaHI	GRCGYC	2 cut(s)	233, 375
BsaI	GGTCTC	1 cut(s)	2555
BsaJI	CCNNGG	5 cut(s)	145, 1042, 1528, 2103, 2389
BsaWI	WCCGGW	3 cut(s)	258, 1415, 1798
BsaXI	ACNNNNNCTCC	2 cut(s)	2763, 2793
Bsc4I	CCNNNNNNNGG	6 cut(s)	158, 545, 569, 661, 1711, 2395
Bse118I	RCCGGY	2 cut(s)	258, 461
Bse1I	ACTGG	7 cut(s)	539, 1226, 1480, 1670, 1811, 2143, 2635
Bse3DI	GCAATG	3 cut(s)	833, 1374, 2064
Bse8I	GATNNNNATC	1 cut(s)	93
BseAI	TCCGGA	1 cut(s)	1798
BseCI	ATCGAT	1 cut(s)	2406
BseDI	CCNNGG	5 cut(s)	145, 1042, 1528, 2103, 2389
BseJI	GATNNNNATC	1 cut(s)	93
BseLI	CCNNNNNNNGG	6 cut(s)	158, 545, 569, 661, 1711, 2395
BseMI	GCAATG	3 cut(s)	833, 1374, 2064
BseMII	CTCAG	4 cut(s)	445, 1116, 2512, 2579
BseNI	ACTGG	7 cut(s)	539, 1226, 1480, 1670, 1811, 2143, 2635
BseRI	GAGGAG	5 cut(s)	134, 2228, 2543, 2686, 2759
BseSI	GKGCMC	1 cut(s)	270
BseXI	GCAGC	6 cut(s)	150, 878, 1166, 1712, 2154, 2157
BseYI	CCCAGC	1 cut(s)	75
BsgI	GTGCAG	1 cut(s)	1095
Bsh1236I	CGCG	1 cut(s)	2665
BshFI	GGCC	7 cut(s)	150, 297, 428, 538, 654, 1919, 2308
BshTI	ACCGGT	1 cut(s)	258
BshVI	ATCGAT	1 cut(s)	2406
BsiHKAI	GWGCWC	1 cut(s)	215
BsiSI	CCGG	6 cut(s)	259, 291, 462, 588, 1416, 1799
BslFI	GGGAC	5 cut(s)	503, 761, 887, 1258, 2731
BslI	CCNNNNNNNGG	6 cut(s)	158, 545, 569, 661, 1711, 2395
BsmAI	GTCTC	6 cut(s)	240, 313, 2348, 2555, 2648, 2688
BsmBI	CGTCTC	2 cut(s)	240, 313
BsmFI	GGGAC	5 cut(s)	503, 761, 887, 1258, 2731
BsmI	GAATGC	1 cut(s)	2066
BsnI	GGCC	7 cut(s)	150, 297, 428, 538, 654, 1919, 2308
Bso31I	GGTCTC	1 cut(s)	2555
Bsp1286I	GDGCHC	2 cut(s)	215, 270
Bsp13I	TCCGGA	1 cut(s)	1798
BspACI	CCGC	9 cut(s)	208, 294, 342, 420, 717, 1152, 1158, 1585, 2702
BspANI	GGCC	7 cut(s)	150, 297, 428, 538, 654, 1919, 2308
BspCNI	CTCAG	4 cut(s)	444, 1115, 2511, 2578
BspDI	ATCGAT	1 cut(s)	2406
BspEI	TCCGGA	1 cut(s)	1798
BspFNI	CGCG	1 cut(s)	2665
BspHI	TCATGA	1 cut(s)	529
BspLI	GGNNCC	8 cut(s)	298, 322, 491, 537, 875, 976, 1999, 2821
BspMI	ACCTGC	1 cut(s)	1397
BspPI	GGATC	3 cut(s)	686, 1779, 1809
BspQI	GCTCTTC	1 cut(s)	458
BspTI	CTTAAG	1 cut(s)	1823
BspTNI	GGTCTC	1 cut(s)	2555
BsrDI	GCAATG	3 cut(s)	833, 1374, 2064
BsrFI	RCCGGY	2 cut(s)	258, 461
BsrI	ACTGG	7 cut(s)	539, 1226, 1480, 1670, 1811, 2143, 2635
BssAI	RCCGGY	2 cut(s)	258, 461
BssECI	CCNNGG	5 cut(s)	145, 1042, 1528, 2103, 2389
BssNI	GRCGYC	2 cut(s)	233, 375
BssSI	CACGAG	1 cut(s)	1992
BssT1I	CCWWGG	2 cut(s)	1528, 2103
Bst2BI	CACGAG	1 cut(s)	1992
Bst6I	CTCTTC	6 cut(s)	458, 686, 844, 2342, 2394, 2769
BstACI	GRCGYC	2 cut(s)	233, 375
BstAFI	CTTAAG	1 cut(s)	1823
BstAPI	GCANNNNNTGC	1 cut(s)	1524
BstBAI	YACGTR	1 cut(s)	2607
BstC8I	GCNNGC	7 cut(s)	266, 334, 512, 579, 721, 2133, 2306
BstDEI	CTNAG	5 cut(s)	406, 431, 1102, 2498, 2565
BstDSI	CCRYGG	1 cut(s)	1042
BstEII	GGTNACC	2 cut(s)	1037, 1280
BstENI	CCTNNNNNAGG	1 cut(s)	2393
BstFNI	CGCG	1 cut(s)	2665
BstMAI	GTCTC	6 cut(s)	240, 313, 2348, 2555, 2648, 2688
BstNSI	RCATGY	2 cut(s)	514, 743
BstPAI	GACNNNNGTC	1 cut(s)	444
BstPI	GGTNACC	2 cut(s)	1037, 1280
BstSFI	CTRYAG	2 cut(s)	886, 1851
BstSLI	GKGCMC	1 cut(s)	270
BstUI	CGCG	1 cut(s)	2665
BstV1I	GCAGC	6 cut(s)	150, 878, 1166, 1712, 2154, 2157
BstV2I	GAAGAC	2 cut(s)	828, 905
BstX2I	RGATCY	1 cut(s)	1801
BstYI	RGATCY	1 cut(s)	1801
Bsu15I	ATCGAT	1 cut(s)	2406
BsuRI	GGCC	7 cut(s)	150, 297, 428, 538, 654, 1919, 2308
BsuTUI	ATCGAT	1 cut(s)	2406
BtgI	CCRYGG	1 cut(s)	1042
BtgZI	GCGATG	3 cut(s)	313, 738, 2163
BtsI	GCAGTG	1 cut(s)	1776
BtsIMutI	CAGTG	5 cut(s)	546, 1491, 1677, 1776, 2330
BveI	ACCTGC	1 cut(s)	1397
Cac8I	GCNNGC	7 cut(s)	266, 334, 512, 579, 721, 2133, 2306
CaiI	CAGNNNCTG	3 cut(s)	1730, 1892, 2021
CciI	TCATGA	1 cut(s)	529
Cfr10I	RCCGGY	2 cut(s)	258, 461
Cfr13I	GGNCC	7 cut(s)	149, 296, 427, 490, 536, 874, 1917
ClaI	ATCGAT	1 cut(s)	2406
CseI	GACGC	3 cut(s)	222, 383, 1368
Csp6I	GTAC	6 cut(s)	1052, 1361, 1620, 1808, 1820, 2178
CspAI	ACCGGT	1 cut(s)	258
CspCI	CAANNNNNGTGG	2 cut(s)	1333, 1368
CviQI	GTAC	6 cut(s)	1052, 1361, 1620, 1808, 1820, 2178
DdeI	CTNAG	5 cut(s)	406, 431, 1102, 2498, 2565
DraI	TTTAAA	2 cut(s)	649, 2229
DraIII	CACNNNGTG	1 cut(s)	2473
DrdI	GACNNNNNNGTC	1 cut(s)	2635
DriI	GACNNNNNGTC	1 cut(s)	1378
DseDI	GACNNNNNNGTC	1 cut(s)	2635
Eam1104I	CTCTTC	6 cut(s)	458, 686, 844, 2342, 2394, 2769
Eam1105I	GACNNNNNGTC	1 cut(s)	1378
EarI	CTCTTC	6 cut(s)	458, 686, 844, 2342, 2394, 2769
Ecl136II	GAGCTC	1 cut(s)	213
Eco130I	CCWWGG	2 cut(s)	1528, 2103
Eco147I	AGGCCT	1 cut(s)	2308
Eco24I	GRGCYC	1 cut(s)	215
Eco31I	GGTCTC	1 cut(s)	2555
Eco32I	GATATC	1 cut(s)	1717
Eco47I	GGWCC	2 cut(s)	490, 874
Eco53kI	GAGCTC	1 cut(s)	213
Eco57I	CTGAAG	7 cut(s)	150, 840, 1089, 1104, 1155, 1998, 2757
Eco91I	GGTNACC	2 cut(s)	1037, 1280
EcoICRI	GAGCTC	1 cut(s)	213
EcoNI	CCTNNNNNAGG	1 cut(s)	2393
EcoO109I	RGGNCCY	2 cut(s)	490, 874
EcoO65I	GGTNACC	2 cut(s)	1037, 1280
EcoRI	GAATTC	1 cut(s)	1063
EcoRV	GATATC	1 cut(s)	1717
EcoT14I	CCWWGG	2 cut(s)	1528, 2103
EcoT22I	ATGCAT	1 cut(s)	2066
EcoT38I	GRGCYC	1 cut(s)	215
ErhI	CCWWGG	2 cut(s)	1528, 2103
Esp3I	CGTCTC	2 cut(s)	240, 313
FalI	AAGNNNNNCTT	6 cut(s)	1003, 1035, 1170, 1202, 2367, 2399
FaqI	GGGAC	5 cut(s)	503, 761, 887, 1258, 2731
FauNDI	CATATG	3 cut(s)	913, 1548, 2074
FbaI	TGATCA	3 cut(s)	1201, 1870, 2077
Fnu4HI	GCNGC	9 cut(s)	164, 295, 343, 892, 1155, 1158, 1701, 2143, 2146
FriOI	GRGCYC	1 cut(s)	215
Fsp4HI	GCNGC	9 cut(s)	164, 295, 343, 892, 1155, 1158, 1701, 2143, 2146
FspBI	CTAG	2 cut(s)	672, 2243
GluI	GCNGC	9 cut(s)	164, 295, 343, 892, 1155, 1158, 1701, 2143, 2146
GsaI	CCCAGC	1 cut(s)	79
GsuI	CTGGAG	2 cut(s)	53, 588
HaeIII	GGCC	7 cut(s)	150, 297, 428, 538, 654, 1919, 2308
HapII	CCGG	6 cut(s)	259, 291, 462, 588, 1416, 1799
HgaI	GACGC	3 cut(s)	222, 383, 1368
Hin1I	GRCGYC	2 cut(s)	233, 375
HincII	GTYRAC	1 cut(s)	756
HindII	GTYRAC	1 cut(s)	756
HindIII	AAGCTT	2 cut(s)	2199, 2315
HinfI	GANTC	8 cut(s)	347, 365, 382, 1133, 1146, 1508, 1675, 2233
HpaI	GTTAAC	1 cut(s)	756
HpaII	CCGG	6 cut(s)	259, 291, 462, 588, 1416, 1799
HphI	GGTGA	6 cut(s)	109, 551, 1049, 1141, 1907, 2626
Hpy166II	GTNNAC	3 cut(s)	756, 1766, 2617
Hpy8I	GTNNAC	3 cut(s)	756, 1766, 2617
Hpy99I	CGWCG	2 cut(s)	320, 443
HpyCH4IV	ACGT	4 cut(s)	1247, 1961, 2606, 2764
HpyF3I	CTNAG	5 cut(s)	406, 431, 1102, 2498, 2565
HpySE526I	ACGT	4 cut(s)	1247, 1961, 2606, 2764
Hsp92I	GRCGYC	2 cut(s)	233, 375
Kpn2I	TCCGGA	1 cut(s)	1798
Ksp22I	TGATCA	3 cut(s)	1201, 1870, 2077
KspAI	GTTAAC	1 cut(s)	756
LguI	GCTCTTC	1 cut(s)	458
LmnI	GCTCC	8 cut(s)	30, 210, 218, 326, 590, 1479, 1562, 2746
Lsp1109I	GCAGC	6 cut(s)	150, 878, 1166, 1712, 2154, 2157
LweI	GCATC	5 cut(s)	636, 1988, 2140, 2251, 2679
MaeI	CTAG	2 cut(s)	672, 2243
MaeII	ACGT	4 cut(s)	1247, 1961, 2606, 2764
MflI	RGATCY	1 cut(s)	1801
MhlI	GDGCHC	2 cut(s)	215, 270
MlyI	GAGTC	1 cut(s)	356
MmeI	TCCRAC	4 cut(s)	339, 1230, 2092, 2528
Mph1103I	ATGCAT	1 cut(s)	2066
MroI	TCCGGA	1 cut(s)	1798
MroXI	GAANNNNTTC	3 cut(s)	1592, 2202, 2424
MslI	CAYNNNNRTG	2 cut(s)	1628, 2162
MspA1I	CMGCKG	1 cut(s)	845
MspCI	CTTAAG	1 cut(s)	1823
MspI	CCGG	6 cut(s)	259, 291, 462, 588, 1416, 1799
Mva1269I	GAATGC	1 cut(s)	2066
MvnI	CGCG	1 cut(s)	2665
NdeI	CATATG	3 cut(s)	913, 1548, 2074
NlaIV	GGNNCC	8 cut(s)	298, 322, 491, 537, 875, 976, 1999, 2821
NmeAIII	GCCGAG	2 cut(s)	126, 2370
NmuCI	GTSAC	5 cut(s)	464, 557, 1037, 1108, 1192
NsiI	ATGCAT	1 cut(s)	2066
NspI	RCATGY	2 cut(s)	514, 743
PaeI	GCATGC	1 cut(s)	514
PagI	TCATGA	1 cut(s)	529
PceI	AGGCCT	1 cut(s)	2308
PciSI	GCTCTTC	1 cut(s)	458
PctI	GAATGC	1 cut(s)	2066
PdmI	GAANNNNTTC	3 cut(s)	1592, 2202, 2424
PfeI	GAWTC	7 cut(s)	365, 382, 1133, 1146, 1508, 1675, 2233
PflMI	CCANNNNNTGG	1 cut(s)	158
PinAI	ACCGGT	1 cut(s)	258
PkrI	GCNGC	9 cut(s)	165, 296, 344, 893, 1156, 1159, 1702, 2144, 2147
PleI	GAGTC	1 cut(s)	355
PpsI	GAGTC	1 cut(s)	355
Ppu21I	YACGTR	1 cut(s)	2607
PpuMI	RGGWCCY	2 cut(s)	490, 874
PshAI	GACNNNNGTC	1 cut(s)	444
PsiI	TTATAA	2 cut(s)	1028, 1883
Psp124BI	GAGCTC	1 cut(s)	215
Psp1406I	AACGTT	1 cut(s)	2764
Psp5II	RGGWCCY	2 cut(s)	490, 874
PspEI	GGTNACC	2 cut(s)	1037, 1280
PspFI	CCCAGC	1 cut(s)	75
PspN4I	GGNNCC	8 cut(s)	298, 322, 491, 537, 875, 976, 1999, 2821
PspPI	GGNCC	7 cut(s)	149, 296, 427, 490, 536, 874, 1917
PspPPI	RGGWCCY	2 cut(s)	490, 874
PstNI	CAGNNNCTG	3 cut(s)	1730, 1892, 2021
PsuI	RGATCY	1 cut(s)	1801
PvuII	CAGCTG	1 cut(s)	845
RsaI	GTAC	6 cut(s)	1053, 1362, 1621, 1809, 1821, 2179
RsaNI	GTAC	6 cut(s)	1052, 1361, 1620, 1808, 1820, 2178
RseI	CAYNNNNRTG	2 cut(s)	1628, 2162
SacI	GAGCTC	1 cut(s)	215
SapI	GCTCTTC	1 cut(s)	458
SatI	GCNGC	9 cut(s)	164, 295, 343, 892, 1155, 1158, 1701, 2143, 2146
Sau96I	GGNCC	7 cut(s)	149, 296, 427, 490, 536, 874, 1917
SchI	GAGTC	1 cut(s)	356
SduI	GDGCHC	2 cut(s)	215, 270
SfaNI	GCATC	5 cut(s)	636, 1988, 2140, 2251, 2679
SfcI	CTRYAG	2 cut(s)	886, 1851
SinI	GGWCC	2 cut(s)	490, 874
SmiMI	CAYNNNNRTG	2 cut(s)	1628, 2162
SmlI	CTYRAG	2 cut(s)	503, 1823
SmoI	CTYRAG	2 cut(s)	503, 1823
SphI	GCATGC	1 cut(s)	514
SseBI	AGGCCT	1 cut(s)	2308
SsiI	CCGC	9 cut(s)	208, 294, 342, 420, 717, 1152, 1158, 1585, 2702
SspI	AATATT	1 cut(s)	106
SspMI	CTAG	2 cut(s)	672, 2243
SstI	GAGCTC	1 cut(s)	215
StuI	AGGCCT	1 cut(s)	2308
StyI	CCWWGG	2 cut(s)	1528, 2103
TaiI	ACGT	4 cut(s)	1250, 1964, 2609, 2767
TaqI	TCGA	3 cut(s)	380, 2406, 2428
TatI	WGTACW	2 cut(s)	1819, 2177
TauI	GCSGC	3 cut(s)	297, 345, 1160
TfiI	GAWTC	7 cut(s)	365, 382, 1133, 1146, 1508, 1675, 2233
TscAI	CASTG	5 cut(s)	546, 1498, 1677, 1783, 2330
TseFI	GTSAC	5 cut(s)	464, 557, 1037, 1108, 1192
TseI	GCWGC	6 cut(s)	163, 891, 1154, 1700, 2142, 2145
Tsp45I	GTSAC	5 cut(s)	464, 557, 1037, 1108, 1192
TspDTI	ATGAA	8 cut(s)	38, 45, 316, 357, 911, 1146, 1319, 2073
TspGWI	ACGGA	4 cut(s)	159, 840, 1069, 2809
TspRI	CASTG	5 cut(s)	546, 1498, 1677, 1783, 2330
Van91I	CCANNNNNTGG	1 cut(s)	158
Vha464I	CTTAAG	1 cut(s)	1823
VpaK11BI	GGWCC	2 cut(s)	490, 874
XagI	CCTNNNNNAGG	1 cut(s)	2393
XapI	RAATTY	3 cut(s)	1063, 1253, 2337
XceI	RCATGY	2 cut(s)	514, 743
XcmI	CCANNNNNNNNNTGG	2 cut(s)	226, 358
XmnI	GAANNNNTTC	3 cut(s)	1592, 2202, 2424
XspI	CTAG	2 cut(s)	672, 2243
Zsp2I	ATGCAT	1 cut(s)	2066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.