pycom02g01110

FAD-NAD(P)-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007219
Physical Location & Seq
Reverse (-)
619466 .. 621182
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 777 bp
ATGGCATCTCAGAATAATGAAGGGATAGGTGGCGGCAGGGGGAGGATGGCGTCTTCTTCTTCTTCTTGTTCTGTTGGGTCACCGAATTCTCTGACTCAGCGCCACCGTCCTCCTCCGAGTCTGAGTCCGAGTTGTGTGAGGTACGCAGTGCTCGGGGCTGGCTTCGCCGGCCTCTCCGTCGCGTGGCACTTGCTAAAGCAGAGTCCGAAAGACTCCAACATCCGCGTCGACATATACGATGAAGCTGGAATCGGTGGCGGCGCGTCCGGGGTTTCGGGTGGGCTTCTTCACCCTTATTCCCCTAAAGCTAAGCTTCTATGGCGTGCTGCCGACTGCTGGAGTGAGTCCTTGAACCTTTTGAGCGCTGCTGAAGCCGCCGTGGCTTCCGTTGCTCAAAATTCTGACGGCCCAACGCCAATTGTTAGAAGAAGGGGCATCTTGAGACCAGCCCTGAGCAGGAAGAACTTGGTATTGTTGAAAGACAATGCCCAGAGTTGCCTTGCTAGTTGCCGAATAGAAACCCTTGACAAGGATGCTGCCCAAAATCTCGTACCCGGCATATCTCCACCTCTTGACGCCTCCTTTTATATGCCTGAAGCTGTTAATATTCATCCTCAACGCTATTTACAGGCACTGTTCTTAGCATGTCAAAATTTGGTGAAGGAACTTTGTGCTTCTGGCTCCGGAGTCAAAGAATTGCATTTGCACAAGAGTTCTGTCCACAAACTCTTAGACCTAGAAGGTAATGACCAACGCCATTGTTTCAAGCTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAO

Protein Analysis

259

Amino Acids

27.42

Weight (kDa)

8.99

Isoelectric Point (pI)

65.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 48 - 219 1.2e-22 FAD dependent oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 228
AccII CGCG 3 cut(s) 182, 225, 263
AccIII TCCGGA 1 cut(s) 683
AciI CCGC 4 cut(s) 33, 223, 258, 375
AcsI RAATTY 3 cut(s) 85, 397, 652
AcuI CTGAAG 2 cut(s) 390, 615
AcyI GRCGYC 2 cut(s) 50, 576
AfaI GTAC 2 cut(s) 143, 552
AfeI AGCGCT 1 cut(s) 364
AfiI CCNNNNNNNGG 4 cut(s) 183, 336, 456, 529
AgsI TTSAA 3 cut(s) 352, 478, 766
AluBI AGCT 5 cut(s) 245, 308, 313, 599, 769
AluI AGCT 5 cut(s) 245, 308, 313, 599, 769
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 1 cut(s) 436
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 1 cut(s) 152
Aor13HI TCCGGA 1 cut(s) 683
Aor51HI AGCGCT 1 cut(s) 364
AoxI GGCC 2 cut(s) 169, 406
ApeKI GCWGC 3 cut(s) 326, 365, 536
ApoI RAATTY 3 cut(s) 85, 397, 652
AspLEI GCGC 3 cut(s) 102, 263, 365
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 268, 555
AsuHPI GGTGA 3 cut(s) 72, 281, 670
AvaI CYCGRG 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 3 cut(s) 313, 352, 523
BccI CCATC 1 cut(s) 40
BceAI ACGGC 2 cut(s) 362, 421
BcnI CCSGG 2 cut(s) 268, 555
BcoDI GTCTC 1 cut(s) 436
BfaI CTAG 2 cut(s) 504, 737
BfoI RGCGCY 2 cut(s) 103, 366
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 6 cut(s) 34, 259, 327, 366, 375, 537
BlpI GCTNAGC 1 cut(s) 309
BlsI GCNGC 6 cut(s) 35, 260, 328, 367, 376, 538
Bme1390I CCNGG 2 cut(s) 268, 555
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 1 cut(s) 682
BmrFI CCNGG 2 cut(s) 268, 555
BmsI GCATC 3 cut(s) 14, 444, 523
BpiI GAAGAC 1 cut(s) 45
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 452
Bpu1102I GCTNAGC 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 460
BpuMI CCSGG 2 cut(s) 268, 555
BsaHI GRCGYC 2 cut(s) 50, 576
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 2 cut(s) 267, 378
BsaWI WCCGGW 1 cut(s) 683
Bsc4I CCNNNNNNNGG 4 cut(s) 183, 336, 456, 529
Bse118I RCCGGY 1 cut(s) 167
BseAI TCCGGA 1 cut(s) 683
BseDI CCNNGG 2 cut(s) 267, 378
BseGI GGATG 4 cut(s) 51, 219, 538, 610
BseLI CCNNNNNNNGG 4 cut(s) 183, 336, 456, 529
BseMII CTCAG 4 cut(s) 23, 110, 113, 443
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 3 cut(s) 313, 352, 523
Bsh1236I CGCG 3 cut(s) 182, 225, 263
BshFI GGCC 2 cut(s) 171, 408
BsiHKAI GWGCWC 1 cut(s) 153
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 4 cut(s) 168, 267, 555, 684
BslI CCNNNNNNNGG 4 cut(s) 183, 336, 456, 529
BsmAI GTCTC 1 cut(s) 436
BsnI GGCC 2 cut(s) 171, 408
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 683
Bsp1720I GCTNAGC 1 cut(s) 309
BspACI CCGC 4 cut(s) 33, 223, 258, 375
BspANI GGCC 2 cut(s) 171, 408
BspCNI CTCAG 4 cut(s) 22, 109, 114, 444
BspEI TCCGGA 1 cut(s) 683
BspFNI CGCG 3 cut(s) 182, 225, 263
BspLI GGNNCC 1 cut(s) 682
BspTNI GGTCTC 1 cut(s) 436
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 267, 378
BssNI GRCGYC 2 cut(s) 50, 576
Bst4CI ACNGT 2 cut(s) 107, 636
BstACI GRCGYC 2 cut(s) 50, 576
BstC8I GCNNGC 3 cut(s) 160, 169, 324
BstDEI CTNAG 7 cut(s) 9, 96, 122, 309, 452, 640, 730
BstDSI CCRYGG 1 cut(s) 378
BstEII GGTNACC 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 4 cut(s) 51, 219, 538, 610
BstFNI CGCG 3 cut(s) 182, 225, 263
BstH2I RGCGCY 2 cut(s) 103, 366
BstHHI GCGC 3 cut(s) 102, 263, 365
BstMAI GTCTC 1 cut(s) 436
BstMWI GCNNNNNNNGC 7 cut(s) 164, 168, 319, 371, 374, 380, 389
BstNSI RCATGY 1 cut(s) 648
BstPI GGTNACC 1 cut(s) 78
BstSCI CCNGG 2 cut(s) 266, 553
BstUI CGCG 3 cut(s) 182, 225, 263
BstV1I GCAGC 3 cut(s) 313, 352, 523
BstV2I GAAGAC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 171, 408
BtgI CCRYGG 1 cut(s) 378
BtsCI GGATG 4 cut(s) 51, 219, 538, 610
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 2 cut(s) 153, 632
Cac8I GCNNGC 3 cut(s) 160, 169, 324
CaiI CAGNNNCTG 1 cut(s) 634
CfoI GCGC 3 cut(s) 102, 263, 365
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 407
CseI GACGC 4 cut(s) 39, 214, 252, 584
Csp6I GTAC 2 cut(s) 142, 551
CviAII CATG 1 cut(s) 645
CviQI GTAC 2 cut(s) 142, 551
DdeI CTNAG 7 cut(s) 9, 96, 122, 309, 452, 640, 730
Eco31I GGTCTC 1 cut(s) 436
Eco47III AGCGCT 1 cut(s) 364
Eco57I CTGAAG 2 cut(s) 390, 615
Eco88I CYCGRG 1 cut(s) 152
Eco91I GGTNACC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO65I GGTNACC 1 cut(s) 78
EcoRI GAATTC 1 cut(s) 85
FaeI CATG 1 cut(s) 648
FaiI YATR 7 cut(s) 233, 235, 319, 560, 588, 590, 646
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FatI CATG 1 cut(s) 644
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 6 cut(s) 34, 259, 327, 366, 375, 537
FokI GGATG 4 cut(s) 58, 206, 545, 597
Fsp4HI GCNGC 6 cut(s) 34, 259, 327, 366, 375, 537
FspBI CTAG 2 cut(s) 504, 737
GlaI GCGC 3 cut(s) 101, 262, 364
GluI GCNGC 6 cut(s) 34, 259, 327, 366, 375, 537
GsuI CTGGAG 1 cut(s) 358
HaeII RGCGCY 2 cut(s) 103, 366
HaeIII GGCC 2 cut(s) 171, 408
HapII CCGG 4 cut(s) 168, 267, 555, 684
HgaI GACGC 4 cut(s) 39, 214, 252, 584
HhaI GCGC 3 cut(s) 102, 263, 365
Hin1I GRCGYC 2 cut(s) 50, 576
Hin1II CATG 1 cut(s) 648
Hin6I GCGC 3 cut(s) 100, 261, 363
HinP1I GCGC 3 cut(s) 100, 261, 363
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 2 cut(s) 311, 767
HinfI GANTC 8 cut(s) 94, 118, 124, 202, 212, 249, 344, 687
HpaII CCGG 4 cut(s) 168, 267, 555, 684
HphI GGTGA 3 cut(s) 72, 281, 670
Hpy166II GTNNAC 2 cut(s) 229, 721
Hpy188I TCNGA 7 cut(s) 12, 93, 117, 123, 129, 207, 403
Hpy188III TCNNGA 3 cut(s) 439, 572, 684
Hpy8I GTNNAC 2 cut(s) 229, 721
Hpy99I CGWCG 2 cut(s) 182, 230
HpyAV CCTTC 4 cut(s) 14, 423, 655, 734
HpyCH4III ACNGT 2 cut(s) 107, 636
HpyCH4V TGCA 2 cut(s) 700, 706
HpyF10VI GCNNNNNNNGC 7 cut(s) 164, 168, 319, 371, 374, 380, 389
HpyF3I CTNAG 7 cut(s) 9, 96, 122, 309, 452, 640, 730
Hsp92I GRCGYC 2 cut(s) 50, 576
Hsp92II CATG 1 cut(s) 648
HspAI GCGC 3 cut(s) 100, 261, 363
Kpn2I TCCGGA 1 cut(s) 683
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 686
Lsp1109I GCAGC 3 cut(s) 313, 352, 523
LweI GCATC 3 cut(s) 14, 444, 523
MaeI CTAG 2 cut(s) 504, 737
MaeIII GTNAC 1 cut(s) 78
MboII GAAGA 7 cut(s) 45, 48, 51, 54, 278, 438, 472
MfeI CAATTG 1 cut(s) 417
MhlI GDGCHC 1 cut(s) 153
MluCI AATT 5 cut(s) 85, 397, 417, 652, 695
MlyI GAGTC 7 cut(s) 88, 127, 133, 206, 211, 353, 696
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 8 cut(s) 36, 120, 123, 132, 182, 579, 589, 624
MroI TCCGGA 1 cut(s) 683
MroNI GCCGGC 1 cut(s) 167
MseI TTAA 2 cut(s) 603, 775
MspI CCGG 4 cut(s) 168, 267, 555, 684
MspR9I CCNGG 2 cut(s) 268, 555
MunI CAATTG 1 cut(s) 417
MvnI CGCG 3 cut(s) 182, 225, 263
MwoI GCNNNNNNNGC 7 cut(s) 164, 168, 319, 371, 374, 380, 389
NaeI GCCGGC 1 cut(s) 169
NciI CCSGG 2 cut(s) 268, 555
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 1 cut(s) 648
NlaIV GGNNCC 1 cut(s) 682
NmuCI GTSAC 1 cut(s) 78
NspI RCATGY 1 cut(s) 648
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PdiI GCCGGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 249
PkrI GCNGC 6 cut(s) 35, 260, 328, 367, 376, 538
PleI GAGTC 7 cut(s) 88, 126, 132, 206, 210, 352, 695
PpsI GAGTC 7 cut(s) 88, 126, 132, 206, 210, 352, 695
PspEI GGTNACC 1 cut(s) 78
PspN4I GGNNCC 1 cut(s) 682
PspPI GGNCC 1 cut(s) 407
PstNI CAGNNNCTG 1 cut(s) 634
RsaI GTAC 2 cut(s) 143, 552
RsaNI GTAC 2 cut(s) 142, 551
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 603, 775
SatI GCNGC 6 cut(s) 34, 259, 327, 366, 375, 537
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 7 cut(s) 88, 127, 133, 206, 211, 353, 696
ScrFI CCNGG 2 cut(s) 268, 555
SduI GDGCHC 1 cut(s) 153
SfaNI GCATC 3 cut(s) 14, 444, 523
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 5 cut(s) 85, 397, 417, 652, 695
SsiI CCGC 4 cut(s) 33, 223, 258, 375
SspI AATATT 1 cut(s) 607
SspMI CTAG 2 cut(s) 504, 737
StyD4I CCNGG 2 cut(s) 266, 553
TaaI ACNGT 2 cut(s) 107, 636
TaqI TCGA 1 cut(s) 228
TasI AATT 5 cut(s) 85, 397, 417, 652, 695
TauI GCSGC 3 cut(s) 36, 261, 377
TfiI GAWTC 1 cut(s) 249
Tru1I TTAA 2 cut(s) 603, 775
Tru9I TTAA 2 cut(s) 603, 775
TscAI CASTG 2 cut(s) 153, 639
TseFI GTSAC 1 cut(s) 78
TseI GCWGC 3 cut(s) 326, 365, 536
Tsp45I GTSAC 1 cut(s) 78
TspDTI ATGAA 3 cut(s) 33, 255, 599
TspGWI ACGGA 2 cut(s) 166, 376
TspRI CASTG 2 cut(s) 153, 639
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 3 cut(s) 85, 397, 652
XceI RCATGY 1 cut(s) 648
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 504, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.