pycom02g01310

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012553
Physical Location & Seq
Reverse (-)
758509 .. 761413
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1473 bp
ATGAAAATAGGAAAAGGATATAATAATATCACAAGAACCCAACAAACTGGTTTCCAGCCACGCTTTCACAGAAAAATCTGGTATTCCTCTCTCTCTCTCTCTCTCTCTCCTCTCTCCTCTCTCCTCTCTGAGACTACAACAACTACCATAACCAGAGGCCCCTATTTGCCACTCACATATTCTCAGAATCTTCTCACCAGTCACCCACAAATCTATATTTTTCTTCCCTCTCTGTTTCTTACTACTTTCGGATTCCCTCAGCAACTCAGCAACACCGCCATTAACAATTTCATCAGCAGCATCATGTGCCTGATAGACGCAGAACAGCTCGGTCAAGAAATGGGGAACATGGAGAATAATAACAACATCGATTCCAACGACAATAACAACAAGCAGTGGTCTCTGCAATTCGACCACCGGACGGAGGAGGAGAATTTGGACCGGGAAACTCCTGCTGTGATGAGCTCAAGTCCTCAGAAGAACATGATAAATTCGTTTCAACTTGAAAGCATAGGCGAGGATACAGTGATTGCAGACAGAAAGCAGAGCCTGTTCAACTTTGTTCCTGCCCTTAGGTCAGGAGAGTGGTCTGATATTGGGGGTCGAACCCATATGGAGGATACTCATATATGCATTGGTGACTTAGCTAAGCAGTTTGGTTATAATCTACTCGGTGGGGAAGCTATCTCTTTCTATGGTGTATTTGATGGCCATGGAGGAAAGGATGCAGCACACTTTGTTCGTGACAATTTACCAAGAGTCATTGTTGAGGATGCTGACTTTCCTTTAGAACTTGAAAAGGTGATAACAAGGTCATTTATGGAGACAGATGCTGCGTTTGCAAAGACGTGTTCTCTTGAGGCTTCCCTTGCTTCTGGCACAACTGCACTCACTGCAATGATATTTGGGAGATCTTTGCTTGTGGCAAATGCGGGAGATTGCCGGGCTGTATTATCGCGATGTGGAGTGGCTGTAGAAATGTCAAGGGATCATAGGCCATGTTGTACAAAAGAAAGAACGCGGATTGAGTCCTTGGGTGGATATATTGATGATGGTTTTCTAAATGGTGAGTTAGGTGTGACCCGTGCATTAGGTAATTGGCATCTTGAAGGACTGAAGGACATGGGTGAAAGTGGCGGACCCTTGAGCGCTGAGCCAGAACTTAAACTGATGACACTGAGCAAAGACGATGAGTTTTTGATAATTGGCAGTGATGGGATATGGGAAATGTTCACCAGCCAAAATGCTGTTGATTTCGCTCGAAGGAGACTGCAAGAGCACAATGATGTGAAGTTGTGCTGCAAGCAAATAGTAGAGGAGGCAATAAAGCGGGGAGCAACGGACAATTTGACGCTTGTCATGGTAAGTTTCCACTTGGAGCCACCTCCACATGTGGTGGTAGAGAGACCTCGAGTCAGACGAAGCATTTCTGCCGAGGGACTTCAGAATCTCAAATTCTTCTTAGACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

491

Amino Acids

54.67

Weight (kDa)

5.34

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 201 - 449 2.8e-63 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 663
AccII CGCG 2 cut(s) 958, 1021
AciI CCGC 5 cut(s) 276, 932, 1021, 1137, 1330
AclWI GGATC 1 cut(s) 996
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 3 cut(s) 433, 490, 1454
AcuI CTGAAG 2 cut(s) 1136, 1427
AfaI GTAC 1 cut(s) 1006
AfeI AGCGCT 1 cut(s) 1150
AfiI CCNNNNNNNGG 3 cut(s) 421, 424, 616
AflIII ACRYGT 2 cut(s) 848, 1390
AgsI TTSAA 5 cut(s) 500, 506, 556, 797, 1109
AhdI GACNNNNNGTC 1 cut(s) 1412
AjiI CACGTC 1 cut(s) 849
AluBI AGCT 4 cut(s) 328, 465, 647, 683
AluI AGCT 4 cut(s) 328, 465, 647, 683
Alw21I GWGCWC 2 cut(s) 467, 1281
Alw26I GTCTC 5 cut(s) 125, 405, 818, 1261, 1399
AlwI GGATC 1 cut(s) 996
AlwNI CAGNNNCTG 2 cut(s) 550, 833
Ama87I CYCGRG 1 cut(s) 1410
Aor51HI AGCGCT 1 cut(s) 1150
AoxI GGCC 3 cut(s) 157, 709, 995
ApeKI GCWGC 4 cut(s) 297, 728, 833, 1299
ApoI RAATTY 3 cut(s) 433, 490, 1454
Asp700I GAANNNNTTC 1 cut(s) 1426
AspLEI GCGC 1 cut(s) 1151
AspS9I GGNCC 3 cut(s) 158, 439, 1139
AsuC2I CCSGG 2 cut(s) 443, 944
AsuHPI GGTGA 7 cut(s) 187, 194, 650, 814, 1079, 1139, 1225
AvaI CYCGRG 1 cut(s) 1410
AvaII GGWCC 2 cut(s) 439, 1139
AxyI CCTNAGG 1 cut(s) 572
BalI TGGCCA 1 cut(s) 711
BanII GRGCYC 1 cut(s) 467
Bbv12I GWGCWC 2 cut(s) 467, 1281
BbvCI CCTCAGC 1 cut(s) 258
BbvI GCAGC 4 cut(s) 309, 740, 820, 1286
BccI CCATC 3 cut(s) 701, 1046, 1208
BciVI GTATCC 2 cut(s) 514, 613
BcnI CCSGG 2 cut(s) 443, 944
BcoDI GTCTC 5 cut(s) 125, 405, 818, 1261, 1399
BfmI CTRYAG 1 cut(s) 972
BfoI RGCGCY 1 cut(s) 1152
BfuI GTATCC 2 cut(s) 514, 613
BglII AGATCT 1 cut(s) 911
BisI GCNGC 4 cut(s) 298, 729, 834, 1300
BlpI GCTNAGC 2 cut(s) 648, 1152
BlsI GCNGC 4 cut(s) 299, 730, 835, 1301
Bme1390I CCNGG 2 cut(s) 443, 944
Bme18I GGWCC 2 cut(s) 439, 1139
BmeRI GACNNNNNGTC 1 cut(s) 1412
BmeT110I CYCGRG 1 cut(s) 1410
BmgBI CACGTC 1 cut(s) 849
BmgT120I GGNCC 3 cut(s) 158, 439, 1139
BmiI GGNNCC 3 cut(s) 160, 1141, 1380
BmrFI CCNGG 2 cut(s) 443, 944
BmsI GCATC 5 cut(s) 309, 715, 763, 820, 1111
BoxI GACNNNNGTC 1 cut(s) 1355
Bpu10I CCTNAGC 1 cut(s) 258
Bpu1102I GCTNAGC 2 cut(s) 648, 1152
BpuEI CTTGAG 3 cut(s) 451, 878, 1165
BpuMI CCSGG 2 cut(s) 443, 944
Bsa29I ATCGAT 1 cut(s) 369
BsaI GGTCTC 2 cut(s) 405, 1399
BsaJI CCNNGG 3 cut(s) 712, 1032, 1434
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 957, 987
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 424, 616
Bse1I ACTGG 2 cut(s) 52, 198
Bse21I CCTNAGG 1 cut(s) 572
Bse3DI GCAATG 1 cut(s) 903
BseCI ATCGAT 1 cut(s) 369
BseDI CCNNGG 3 cut(s) 712, 1032, 1434
BseGI GGATG 2 cut(s) 730, 778
BseLI CCNNNNNNNGG 3 cut(s) 421, 424, 616
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 7 cut(s) 120, 197, 272, 280, 488, 1143, 1169
BseNI ACTGG 2 cut(s) 52, 198
BseRI GAGGAG 6 cut(s) 99, 106, 113, 440, 443, 1331
BseXI GCAGC 4 cut(s) 309, 740, 820, 1286
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 2 cut(s) 958, 1021
BshFI GGCC 3 cut(s) 159, 711, 997
BshVI ATCGAT 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 467, 1281
BsiHKCI CYCGRG 1 cut(s) 1410
BsiSI CCGG 3 cut(s) 418, 442, 943
BslFI GGGAC 1 cut(s) 1452
BslI CCNNNNNNNGG 3 cut(s) 421, 424, 616
BsmAI GTCTC 5 cut(s) 125, 405, 818, 1261, 1399
BsmFI GGGAC 1 cut(s) 1452
BsnI GGCC 3 cut(s) 159, 711, 997
Bso31I GGTCTC 2 cut(s) 405, 1399
BsoBI CYCGRG 1 cut(s) 1410
Bsp1286I GDGCHC 2 cut(s) 467, 1281
Bsp1407I TGTACA 1 cut(s) 1004
Bsp143I GATC 2 cut(s) 911, 988
Bsp1720I GCTNAGC 2 cut(s) 648, 1152
Bsp19I CCATGG 1 cut(s) 712
Bsp68I TCGCGA 1 cut(s) 958
BspACI CCGC 5 cut(s) 276, 932, 1021, 1137, 1330
BspANI GGCC 3 cut(s) 159, 711, 997
BspCNI CTCAG 7 cut(s) 121, 196, 271, 279, 487, 1144, 1170
BspDI ATCGAT 1 cut(s) 369
BspFNI CGCG 2 cut(s) 958, 1021
BspLI GGNNCC 3 cut(s) 160, 1141, 1380
BspPI GGATC 1 cut(s) 996
BspTNI GGTCTC 2 cut(s) 405, 1399
BsrDI GCAATG 1 cut(s) 903
BsrGI TGTACA 1 cut(s) 1004
BsrI ACTGG 2 cut(s) 52, 198
BssECI CCNNGG 3 cut(s) 712, 1032, 1434
BssMI GATC 2 cut(s) 911, 988
BssT1I CCWWGG 2 cut(s) 712, 1032
Bst4CI ACNGT 1 cut(s) 526
BstAPI GCANNNNNTGC 2 cut(s) 306, 893
BstAUI TGTACA 1 cut(s) 1004
BstC8I GCNNGC 1 cut(s) 1304
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 2 cut(s) 730, 778
BstFNI CGCG 2 cut(s) 958, 1021
BstH2I RGCGCY 1 cut(s) 1152
BstHHI GCGC 1 cut(s) 1151
BstKTI GATC 2 cut(s) 914, 991
BstMAI GTCTC 5 cut(s) 125, 405, 818, 1261, 1399
BstMBI GATC 2 cut(s) 911, 988
BstMWI GCNNNNNNNGC 4 cut(s) 306, 839, 869, 893
BstNSI RCATGY 1 cut(s) 1394
BstPAI GACNNNNGTC 1 cut(s) 1355
BstSCI CCNGG 2 cut(s) 441, 942
BstSFI CTRYAG 1 cut(s) 972
BstUI CGCG 2 cut(s) 958, 1021
BstV1I GCAGC 4 cut(s) 309, 740, 820, 1286
BstX2I RGATCY 1 cut(s) 911
BstXI CCANNNNNNTGG 1 cut(s) 47
BstYI RGATCY 1 cut(s) 911
Bsu15I ATCGAT 1 cut(s) 369
Bsu36I CCTNAGG 1 cut(s) 572
BsuI GTATCC 2 cut(s) 514, 613
BsuRI GGCC 3 cut(s) 159, 711, 997
BsuTUI ATCGAT 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 712
BtgZI GCGATG 1 cut(s) 973
BtrI CACGTC 1 cut(s) 849
BtsCI GGATG 2 cut(s) 730, 778
BtsI GCAGTG 3 cut(s) 401, 891, 1216
BtsIMutI CAGTG 5 cut(s) 401, 531, 891, 1175, 1216
BtuMI TCGCGA 1 cut(s) 958
Cac8I GCNNGC 1 cut(s) 1304
CaiI CAGNNNCTG 2 cut(s) 550, 833
CfoI GCGC 1 cut(s) 1151
Cfr13I GGNCC 3 cut(s) 158, 439, 1139
ClaI ATCGAT 1 cut(s) 369
CseI GACGC 2 cut(s) 326, 1360
Csp6I GTAC 1 cut(s) 1005
CviAII CATG 8 cut(s) 304, 349, 484, 713, 999, 1123, 1360, 1391
CviQI GTAC 1 cut(s) 1005
DpnI GATC 2 cut(s) 913, 990
DpnII GATC 2 cut(s) 911, 988
DriI GACNNNNNGTC 1 cut(s) 1412
EaeI YGGCCR 1 cut(s) 709
Eam1105I GACNNNNNGTC 1 cut(s) 1412
EciI GGCGGA 1 cut(s) 1152
Ecl136II GAGCTC 1 cut(s) 465
Eco130I CCWWGG 2 cut(s) 712, 1032
Eco24I GRGCYC 1 cut(s) 467
Eco31I GGTCTC 2 cut(s) 405, 1399
Eco47I GGWCC 2 cut(s) 439, 1139
Eco47III AGCGCT 1 cut(s) 1150
Eco53kI GAGCTC 1 cut(s) 465
Eco57I CTGAAG 2 cut(s) 1136, 1427
Eco81I CCTNAGG 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 1410
EcoICRI GAGCTC 1 cut(s) 465
EcoO109I RGGNCCY 1 cut(s) 158
EcoT14I CCWWGG 2 cut(s) 712, 1032
EcoT22I ATGCAT 1 cut(s) 635
EcoT38I GRGCYC 1 cut(s) 467
ErhI CCWWGG 2 cut(s) 712, 1032
FaeI CATG 8 cut(s) 307, 352, 487, 716, 1002, 1126, 1363, 1394
FaqI GGGAC 1 cut(s) 1452
FatI CATG 8 cut(s) 303, 348, 483, 712, 998, 1122, 1359, 1390
FauI CCCGC 2 cut(s) 925, 1323
FauNDI CATATG 1 cut(s) 612
Fnu4HI GCNGC 4 cut(s) 298, 729, 834, 1300
FokI GGATG 2 cut(s) 737, 785
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 4 cut(s) 298, 729, 834, 1300
GlaI GCGC 1 cut(s) 1150
GluI GCNGC 4 cut(s) 298, 729, 834, 1300
HaeII RGCGCY 1 cut(s) 1152
HaeIII GGCC 3 cut(s) 159, 711, 997
HapII CCGG 3 cut(s) 418, 442, 943
HgaI GACGC 2 cut(s) 326, 1360
HhaI GCGC 1 cut(s) 1151
Hin1II CATG 8 cut(s) 307, 352, 487, 716, 1002, 1126, 1363, 1394
Hin6I GCGC 1 cut(s) 1149
HinP1I GCGC 1 cut(s) 1149
HinfI GANTC 7 cut(s) 187, 252, 371, 759, 1028, 1413, 1447
HpaII CCGG 3 cut(s) 418, 442, 943
HphI GGTGA 7 cut(s) 187, 194, 650, 814, 1079, 1139, 1225
Hpy166II GTNNAC 1 cut(s) 1233
Hpy188I TCNGA 7 cut(s) 130, 186, 251, 477, 592, 1418, 1446
Hpy188III TCNNGA 6 cut(s) 335, 579, 743, 857, 957, 1106
Hpy8I GTNNAC 1 cut(s) 1233
HpyAV CCTTC 3 cut(s) 1103, 1111, 1257
HpyCH4III ACNGT 1 cut(s) 526
HpyCH4IV ACGT 1 cut(s) 848
HpyF10VI GCNNNNNNNGC 4 cut(s) 306, 839, 869, 893
HpySE526I ACGT 1 cut(s) 848
Hsp92II CATG 8 cut(s) 307, 352, 487, 716, 1002, 1126, 1363, 1394
HspAI GCGC 1 cut(s) 1149
Kzo9I GATC 2 cut(s) 911, 988
LmnI GCTCC 2 cut(s) 1334, 1378
Lsp1109I GCAGC 4 cut(s) 309, 740, 820, 1286
LweI GCATC 5 cut(s) 309, 715, 763, 820, 1111
MaeII ACGT 1 cut(s) 848
MaeIII GTNAC 4 cut(s) 200, 638, 743, 1078
MalI GATC 2 cut(s) 913, 990
MboI GATC 2 cut(s) 911, 988
MboII GAAGA 4 cut(s) 182, 215, 490, 1450
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 2 cut(s) 467, 1281
MlsI TGGCCA 1 cut(s) 711
MluCI AATT 9 cut(s) 286, 407, 433, 490, 748, 1096, 1203, 1345, 1454
MluNI TGGCCA 1 cut(s) 711
MlyI GAGTC 3 cut(s) 768, 1037, 1422
MmeI TCCRAC 1 cut(s) 399
Mox20I TGGCCA 1 cut(s) 711
Mph1103I ATGCAT 1 cut(s) 635
MroXI GAANNNNTTC 1 cut(s) 1426
MscI TGGCCA 1 cut(s) 711
MseI TTAA 2 cut(s) 282, 1164
MslI CAYNNNNRTG 2 cut(s) 896, 1284
Msp20I TGGCCA 1 cut(s) 711
MspI CCGG 3 cut(s) 418, 442, 943
MspR9I CCNGG 2 cut(s) 443, 944
MvnI CGCG 2 cut(s) 958, 1021
MwoI GCNNNNNNNGC 4 cut(s) 306, 839, 869, 893
NciI CCSGG 2 cut(s) 443, 944
NcoI CCATGG 1 cut(s) 712
NdeI CATATG 1 cut(s) 612
NdeII GATC 2 cut(s) 911, 988
NlaIII CATG 8 cut(s) 307, 352, 487, 716, 1002, 1126, 1363, 1394
NlaIV GGNNCC 3 cut(s) 160, 1141, 1380
NmeAIII GCCGAG 1 cut(s) 1459
NmuCI GTSAC 4 cut(s) 200, 638, 743, 1078
NruI TCGCGA 1 cut(s) 958
NsiI ATGCAT 1 cut(s) 635
NspI RCATGY 1 cut(s) 1394
PaeR7I CTCGAG 1 cut(s) 1410
PciI ACATGT 1 cut(s) 1390
PcsI WCGNNNNNNNCGW 2 cut(s) 375, 1417
PdmI GAANNNNTTC 1 cut(s) 1426
PfeI GAWTC 4 cut(s) 187, 252, 371, 1447
PkrI GCNGC 4 cut(s) 299, 730, 835, 1301
PleI GAGTC 3 cut(s) 767, 1036, 1421
PpsI GAGTC 3 cut(s) 767, 1036, 1421
PscI ACATGT 1 cut(s) 1390
PshAI GACNNNNGTC 1 cut(s) 1355
PsiI TTATAA 1 cut(s) 663
Psp124BI GAGCTC 1 cut(s) 467
PspN4I GGNNCC 3 cut(s) 160, 1141, 1380
PspPI GGNCC 3 cut(s) 158, 439, 1139
PspXI VCTCGAGB 1 cut(s) 1410
PstNI CAGNNNCTG 2 cut(s) 550, 833
PsuI RGATCY 1 cut(s) 911
RruI TCGCGA 1 cut(s) 958
RsaI GTAC 1 cut(s) 1006
RsaNI GTAC 1 cut(s) 1005
RseI CAYNNNNRTG 2 cut(s) 896, 1284
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 2 cut(s) 282, 1164
SatI GCNGC 4 cut(s) 298, 729, 834, 1300
Sau3AI GATC 2 cut(s) 911, 988
Sau96I GGNCC 3 cut(s) 158, 439, 1139
SchI GAGTC 3 cut(s) 768, 1037, 1422
ScrFI CCNGG 2 cut(s) 443, 944
SduI GDGCHC 2 cut(s) 467, 1281
SfaNI GCATC 5 cut(s) 309, 715, 763, 820, 1111
SfcI CTRYAG 1 cut(s) 972
Sfr274I CTCGAG 1 cut(s) 1410
SinI GGWCC 2 cut(s) 439, 1139
SlaI CTCGAG 1 cut(s) 1410
SmiMI CAYNNNNRTG 2 cut(s) 896, 1284
SmlI CTYRAG 4 cut(s) 466, 857, 1144, 1410
SmoI CTYRAG 4 cut(s) 466, 857, 1144, 1410
Sse9I AATT 9 cut(s) 286, 407, 433, 490, 748, 1096, 1203, 1345, 1454
SsiI CCGC 5 cut(s) 276, 932, 1021, 1137, 1330
SstI GAGCTC 1 cut(s) 467
StyD4I CCNGG 2 cut(s) 441, 942
StyI CCWWGG 2 cut(s) 712, 1032
TaaI ACNGT 1 cut(s) 526
TaiI ACGT 1 cut(s) 851
TaqI TCGA 5 cut(s) 369, 411, 604, 1261, 1411
TaqII GACCGA 1 cut(s) 320
TasI AATT 9 cut(s) 286, 407, 433, 490, 748, 1096, 1203, 1345, 1454
TatI WGTACW 1 cut(s) 1004
TfiI GAWTC 4 cut(s) 187, 252, 371, 1447
Tru1I TTAA 2 cut(s) 282, 1164
Tru9I TTAA 2 cut(s) 282, 1164
TscAI CASTG 5 cut(s) 401, 531, 898, 1182, 1216
TseFI GTSAC 4 cut(s) 200, 638, 743, 1078
TseI GCWGC 4 cut(s) 297, 728, 833, 1299
Tsp45I GTSAC 4 cut(s) 200, 638, 743, 1078
TspDTI ATGAA 2 cut(s) 17, 280
TspGWI ACGGA 2 cut(s) 437, 1355
TspRI CASTG 5 cut(s) 401, 531, 898, 1182, 1216
VpaK11BI GGWCC 2 cut(s) 439, 1139
XapI RAATTY 3 cut(s) 433, 490, 1454
XceI RCATGY 1 cut(s) 1394
XhoI CTCGAG 1 cut(s) 1410
XmnI GAANNNNTTC 1 cut(s) 1426
Zsp2I ATGCAT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.