pycom02g03170

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008099
Physical Location & Seq
Reverse (-)
2059362 .. 2060338
977 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGATCAGCACAAAACTTTCAGACCAAATCTTCTGGCCATTAATCCCCGCCGCGTGTGTCAGTTTCCGGCGGCCGATAACCACTAAGTCCAAACTACTGCCCTACTCACGCCCTAAATCTTTGAAACTCAGACACTTAGCCCAAAACTCCAAATGGGCTGTTAGGCTAAGCTTAGTGGACCAAAGCCCGCCAAAATCGACGTTTGACGTGGAACGGTTAGTAGAGTTTTTGTACGAGGATCTAGTTCATCTTTTTGATGATCAGGGAATTGATCGGACGGCGTGTGATGAGCGTGTGAAGTTCAGGTACCCAATCACCAAGCATGAGACTGTAACTGGGTACTTGTTGAACATAGCCTTCTTGAAGATTGTCTTCATGCCTAAGTTTCAGTTGCACTGGGTCAAACAGACAGGACCATATGAAATCACTAGAAGGTGGACTATGGTTATGAAGTTCATCCCTCTACCCTGGAAACCAGAACTGATCTTTAGAGGAACCTCTGTCATGGGCATCAACCCCGAGACCGGGAAGTTCTGCAGCCATGTGGACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358 SOUL

Protein Analysis

185

Amino Acids

21.59

Weight (kDa)

9.68

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 72 - 181 7.9e-24 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 306
AccB1I GGYRCC 1 cut(s) 306
AccII CGCG 1 cut(s) 53
AciI CCGC 4 cut(s) 48, 51, 70, 188
AclWI GGATC 1 cut(s) 246
AcoI YGGCCR 2 cut(s) 35, 71
AfaI GTAC 3 cut(s) 233, 308, 341
AfiI CCNNNNNNNGG 2 cut(s) 524, 525
AgsI TTSAA 3 cut(s) 124, 349, 364
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 1 cut(s) 467
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 320, 515
AlwI GGATC 1 cut(s) 246
Ama87I CYCGRG 1 cut(s) 518
AoxI GGCC 2 cut(s) 35, 71
ApeKI GCWGC 1 cut(s) 537
AseI ATTAAT 1 cut(s) 41
Asp718I GGTACC 1 cut(s) 306
AspS9I GGNCC 2 cut(s) 178, 413
AsuC2I CCSGG 1 cut(s) 526
AsuHPI GGTGA 1 cut(s) 307
AvaI CYCGRG 1 cut(s) 518
AvaII GGWCC 2 cut(s) 178, 413
BalI TGGCCA 1 cut(s) 37
BanI GGYRCC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
BbsI GAAGAC 1 cut(s) 364
BbvI GCAGC 1 cut(s) 549
BceAI ACGGC 1 cut(s) 294
BciT130I CCWGG 1 cut(s) 469
BclI TGATCA 2 cut(s) 3, 259
BcnI CCSGG 1 cut(s) 526
BcoDI GTCTC 2 cut(s) 320, 515
BfaI CTAG 2 cut(s) 242, 429
BfmI CTRYAG 1 cut(s) 535
BisI GCNGC 3 cut(s) 51, 71, 538
BlpI GCTNAGC 1 cut(s) 167
BlsI GCNGC 3 cut(s) 52, 72, 539
Bme1390I CCNGG 2 cut(s) 469, 526
Bme18I GGWCC 2 cut(s) 178, 413
BmeT110I CYCGRG 1 cut(s) 518
BmgBI CACGTC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 178, 413
BmiI GGNNCC 2 cut(s) 308, 496
BmrFI CCNGG 2 cut(s) 469, 526
BmrI ACTGGG 2 cut(s) 345, 406
BmsI GCATC 1 cut(s) 519
BmuI ACTGGG 2 cut(s) 345, 406
BpiI GAAGAC 1 cut(s) 364
Bpu1102I GCTNAGC 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 526
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 467
Bsc4I CCNNNNNNNGG 2 cut(s) 524, 525
Bse1I ACTGG 2 cut(s) 340, 401
BseBI CCWGG 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 467
BseGI GGATG 1 cut(s) 456
BseLI CCNNNNNNNGG 2 cut(s) 524, 525
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 2 cut(s) 340, 401
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 53
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 2 cut(s) 37, 73
BshNI GGYRCC 1 cut(s) 306
BsiEI CGRYCG 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 518
BsiSI CCGG 2 cut(s) 67, 525
BslI CCNNNNNNNGG 2 cut(s) 524, 525
BsmAI GTCTC 2 cut(s) 320, 515
BsnI GGCC 2 cut(s) 37, 73
Bso31I GGTCTC 1 cut(s) 515
BsoBI CYCGRG 1 cut(s) 518
Bsp143I GATC 5 cut(s) 3, 238, 259, 271, 483
Bsp1720I GCTNAGC 1 cut(s) 167
BspACI CCGC 4 cut(s) 48, 51, 70, 188
BspANI GGCC 2 cut(s) 37, 73
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 1 cut(s) 53
BspLI GGNNCC 2 cut(s) 308, 496
BspMAI CTGCAG 1 cut(s) 539
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 306
BspTNI GGTCTC 1 cut(s) 515
BsrI ACTGG 2 cut(s) 340, 401
BssECI CCNNGG 1 cut(s) 467
BssMI GATC 5 cut(s) 3, 238, 259, 271, 483
Bst2UI CCWGG 1 cut(s) 469
Bst4CI ACNGT 2 cut(s) 216, 331
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 6 cut(s) 84, 128, 136, 167, 172, 381
BstF5I GGATG 1 cut(s) 456
BstFNI CGCG 1 cut(s) 53
BstKTI GATC 5 cut(s) 6, 241, 262, 274, 486
BstMAI GTCTC 2 cut(s) 320, 515
BstMBI GATC 5 cut(s) 3, 238, 259, 271, 483
BstMCI CGRYCG 1 cut(s) 74
BstNI CCWGG 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 467, 524
BstSFI CTRYAG 1 cut(s) 535
BstUI CGCG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 549
BstV2I GAAGAC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BstZI CGGCCG 1 cut(s) 71
BsuRI GGCC 2 cut(s) 37, 73
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 394
Cac8I GCNNGC 1 cut(s) 188
Cfr13I GGNCC 2 cut(s) 178, 413
Csp6I GTAC 3 cut(s) 232, 307, 340
CviAII CATG 4 cut(s) 323, 376, 505, 542
CviJI RGCY 9 cut(s) 37, 73, 140, 158, 166, 171, 186, 356, 540
CviKI_1 RGCY 9 cut(s) 37, 73, 140, 158, 166, 171, 186, 356, 540
CviQI GTAC 3 cut(s) 232, 307, 340
DdeI CTNAG 6 cut(s) 84, 128, 136, 167, 172, 381
DpnI GATC 5 cut(s) 5, 240, 261, 273, 485
DpnII GATC 5 cut(s) 3, 238, 259, 271, 483
EaeI YGGCCR 2 cut(s) 35, 71
EagI CGGCCG 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 71
Eco31I GGTCTC 1 cut(s) 515
Eco47I GGWCC 2 cut(s) 178, 413
Eco52I CGGCCG 1 cut(s) 71
Eco88I CYCGRG 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 467
FaeI CATG 4 cut(s) 326, 379, 508, 545
FaiI YATR 9 cut(s) 324, 353, 377, 418, 420, 443, 449, 506, 543
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FatI CATG 4 cut(s) 322, 375, 504, 541
FauI CCCGC 2 cut(s) 55, 195
FauNDI CATATG 1 cut(s) 418
FbaI TGATCA 2 cut(s) 3, 259
Fnu4HI GCNGC 3 cut(s) 51, 71, 538
FokI GGATG 1 cut(s) 443
Fsp4HI GCNGC 3 cut(s) 51, 71, 538
FspBI CTAG 2 cut(s) 242, 429
GluI GCNGC 3 cut(s) 51, 71, 538
HaeIII GGCC 2 cut(s) 37, 73
HapII CCGG 2 cut(s) 67, 525
Hin1II CATG 4 cut(s) 326, 379, 508, 545
HindIII AAGCTT 1 cut(s) 169
HpaII CCGG 2 cut(s) 67, 525
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 3 cut(s) 178, 438, 547
Hpy188I TCNGA 4 cut(s) 22, 131, 276, 554
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 3 cut(s) 178, 438, 547
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 367, 426
HpyCH4III ACNGT 2 cut(s) 216, 331
HpyCH4IV ACGT 2 cut(s) 200, 207
HpyCH4V TGCA 2 cut(s) 394, 537
HpyF3I CTNAG 6 cut(s) 84, 128, 136, 167, 172, 381
HpySE526I ACGT 2 cut(s) 200, 207
Hsp92II CATG 4 cut(s) 326, 379, 508, 545
KpnI GGTACC 1 cut(s) 310
Ksp22I TGATCA 2 cut(s) 3, 259
Kzo9I GATC 5 cut(s) 3, 238, 259, 271, 483
Lsp1109I GCAGC 1 cut(s) 549
LweI GCATC 1 cut(s) 519
MaeI CTAG 2 cut(s) 242, 429
MaeII ACGT 2 cut(s) 200, 207
MaeIII GTNAC 1 cut(s) 331
MalI GATC 5 cut(s) 5, 240, 261, 273, 485
MboI GATC 5 cut(s) 3, 238, 259, 271, 483
MboII GAAGA 3 cut(s) 22, 364, 376
MflI RGATCY 1 cut(s) 238
MlsI TGGCCA 1 cut(s) 37
MluCI AATT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 37
MnlI CCTC 4 cut(s) 229, 471, 485, 508
Mox20I TGGCCA 1 cut(s) 37
MscI TGGCCA 1 cut(s) 37
MseI TTAA 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 37
MspI CCGG 2 cut(s) 67, 525
MspR9I CCNGG 2 cut(s) 469, 526
MvaI CCWGG 1 cut(s) 469
MvnI CGCG 1 cut(s) 53
NciI CCSGG 1 cut(s) 526
NdeI CATATG 1 cut(s) 418
NdeII GATC 5 cut(s) 3, 238, 259, 271, 483
NlaIII CATG 4 cut(s) 326, 379, 508, 545
NlaIV GGNNCC 2 cut(s) 308, 496
PkrI GCNGC 3 cut(s) 52, 72, 539
PshBI ATTAAT 1 cut(s) 41
Psp6I CCWGG 1 cut(s) 467
PspGI CCWGG 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 308, 496
PspPI GGNCC 2 cut(s) 178, 413
PstI CTGCAG 1 cut(s) 539
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 3 cut(s) 233, 308, 341
RsaNI GTAC 3 cut(s) 232, 307, 340
SaqAI TTAA 1 cut(s) 41
SatI GCNGC 3 cut(s) 51, 71, 538
Sau3AI GATC 5 cut(s) 3, 238, 259, 271, 483
Sau96I GGNCC 2 cut(s) 178, 413
ScrFI CCNGG 2 cut(s) 469, 526
SetI ASST 6 cut(s) 173, 203, 210, 308, 437, 500
SfaNI GCATC 1 cut(s) 519
SfcI CTRYAG 1 cut(s) 535
SinI GGWCC 2 cut(s) 178, 413
Sse9I AATT 1 cut(s) 267
SsiI CCGC 4 cut(s) 48, 51, 70, 188
SspMI CTAG 2 cut(s) 242, 429
StyD4I CCNGG 2 cut(s) 467, 524
TaaI ACNGT 2 cut(s) 216, 331
TaiI ACGT 2 cut(s) 203, 210
TaqI TCGA 1 cut(s) 197
TasI AATT 1 cut(s) 267
TauI GCSGC 2 cut(s) 53, 73
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TscAI CASTG 1 cut(s) 401
TseI GCWGC 1 cut(s) 537
TspDTI ATGAA 5 cut(s) 236, 364, 435, 445, 464
TspRI CASTG 1 cut(s) 401
VpaK11BI GGWCC 2 cut(s) 178, 413
VspI ATTAAT 1 cut(s) 41
XspI CTAG 2 cut(s) 242, 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.