pycom02g06500

NPR1/NIM1 like defence protein C terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005113
Physical Location & Seq
Reverse (-)
4518229 .. 4520778
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06500.1

Sequence Viewer

Length: 1773 bp
ATGGGTGACGATCACTTCGTTAGCAACAGCCAATCCAACAGCAGCAACATCTCCTGCATTGCAGTACCCCACAACCGTCCCCCTCCCGACATCTCCGCCCTTGCTCGCCTCTCCGACAACCTAGAATCGATTGTCGACTCCACAGACTTTGACTACTTCCCCGATGCCAAGATCGTCGTCAACGCCGGCCGGGAAGTTCCCGTCCACCGCTGCGTTCTGTCTGCGAGGAGCGAGTTTTTCAAGAACGTGTTCTCGGCGAAGGACAGAGGATCATCAGCAGCGTCGTCGTTGTCGTCGGTTGCGGCTCCTCGATTTGAATTGAAGGAGCTCGCCAAGGAATACGAAGTCGGACTGGACGCGCTCCTCGCCGTTTTGGGGTATTTGTACAGCGGGAAGCTCAGGTCGCTGCCCAAAGGCGTTTGCGTCTGCGTGGACGACGCTTGCGCACATGTCGCTTGCCGGCCGGTGGTTGATTTCATGGTGGAGGTTCTGTATGCTTCTGCAACTTTTCAACTCTCTGAATTGGGCTCTCTTTATCAGAGGCATCTGCTAGATATTCTTGAGAAAGTCGCCATAGATGACATACTGGTGGTGCTGGACGTCGCCAACATGTGCGGCAAAGCTTGCGAGAGATTGGCGGGGAGGTGCGTTGAGATGATAGTCAAGTCCGACGCCGACGTTATAACTCTTGAGAAGGCCCTGCCGCAACACTCCGTGAAGCAGATTACTGATTCCCGCCTGGCGCTCGGCTTGGACAGGCCTCAACAGTTCCACTCTGGTGCTAGTACTAGTCATTTCCCAGATAAACACACCAAGAGGATACACAGGGCTTTGGATTCGGATGATGTCGAGTTGGTGCGAATGCTGCTGAAAGAAGGGAATACCAATCTCGACGACACCTTTGCACTGCACTACGCTGTCGCATACTGCGATGCAAAGACCACAACGGAGCTGCTTGATCTTGGAATTGCATATGTTAATCGTAGGAATCCGAGGGGGTACACTGTGTTGCATGTTGCGGCGATGAGGAAGGAGCCTAAGATCATTGTGTCCCTTCTCACGAAGGGTGCTCGCCCTTCGGATCTTACGCTGGATGGGAGGAAAGCTGTGCAGATCACTAAGCGGCTTACCAGGGCTGTGGATTTTTTTAAGTCTAATGACGAGGGAAAGTCTTCTTCCAATGATCGCTTGTGCATAGAGATTTTGGAGCAAGCGGAAAGAAGGGAACCGTTGCTTGGAGAAGCCTCGCTTTCGCTTGCTACGGCCGGCGATGATCTGCGCATGAAACTTTTGTACCTTGAGAACAGAGTTGGACTTGCAAGATATCTGTTCCCCATGGAAGCCAAGGTTGTAATGGATACCACCCAAATCGATGGCGATGCTTCCGAGTTTCCGAAGGGTCTGGCCAGCTCTCAGAGGACGATGGTGGACTTAAACGAGGCACCCTTCAAAATCAAGAAAGAGCACCTTAGCCGTCTACAAGCTCTATCTCGAGCTGTGGAAATAGGGAAACGCTTCTTTCCCCGGTGCTCGGAAGTACTGAATAACATCATGGACGGCGATGACATATCACAGATTCAATACACCTGGAACGACACGCCAGAATTGCGGCTCACGAAGAAACGAAGGTACATGGAACTCCAGGAAGTACTAAGCCAGGCATTCGACAAGGACAAAGAGGAGTTCGATCGGTCTGGAATATCTTCTTCTTCGTCATCCACATCGATAGGTGTGGTGAGGTCTAATGGTGGTACGATCACCATCAAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001666 GO:0002376 GO:0002682 GO:0002831 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009266 GO:0009408 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009620 GO:0009625 GO:0009627 GO:0009628 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009814 GO:0009862 GO:0009863 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010033 GO:0010112 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019219 GO:0019222 GO:0023051 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031348 GO:0032101 GO:0032870 GO:0035690 GO:0036293 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043900 GO:0044424 GO:0044464 GO:0045087 GO:0045088 GO:0045893 GO:0045935 GO:0046677 GO:0048518 GO:0048519 GO:0048522 GO:0048583 GO:0048585 GO:0050776 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070482 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071407 GO:0071446 GO:0071495 GO:0080027 GO:0080090 GO:0080134 GO:0098542 GO:1901698 GO:1901700 GO:1901701 GO:1902680 GO:1903506 GO:1903508 GO:2000022 GO:2000031 GO:2000112 GO:2001023 GO:2001038 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Ank Ank_2 Ank_4 BTB DUF3420 NPR1_like_C

Protein Analysis

591

Amino Acids

65.4

Weight (kDa)

6.27

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 53 - 132 3.6e-08 BTB/POZ domain
DUF3420 PF11900 226 - 275 8.1e-08 Domain of unknown function (DUF3420)
Ank_2 PF12796 274 - 359 2.9e-08 Ankyrin repeats (3 copies)
NPR1_like_C PF12313 374 - 574 6.8e-80 NPR1/NIM1 like defence protein C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AatII GACGTC 1 cut(s) 603
Acc16I TGCGCA 2 cut(s) 445, 1280
AccB1I GGYRCC 1 cut(s) 1441
AccI GTMKAC 2 cut(s) 135, 1477
AccII CGCG 1 cut(s) 359
AclWI GGATC 2 cut(s) 277, 1089
AcoI YGGCCR 4 cut(s) 187, 461, 1263, 1404
AcyI GRCGYC 2 cut(s) 600, 672
AdeI CACNNNGTG 1 cut(s) 715
AfaI GTAC 9 cut(s) 66, 386, 787, 1001, 1295, 1539, 1631, 1650, 1753
AfiI CCNNNNNNNGG 4 cut(s) 375, 466, 1235, 1531
AflIII ACRYGT 3 cut(s) 246, 448, 609
AgsI TTSAA 6 cut(s) 241, 317, 322, 512, 1450, 1580
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 5 cut(s) 738, 1130, 1586, 1641, 1656
AjuI GAANNNNNNNTTGG 2 cut(s) 1218, 1250
AleI CACNNNNGTG 1 cut(s) 777
AluBI AGCT 8 cut(s) 328, 397, 623, 952, 1106, 1410, 1484, 1496
AluI AGCT 8 cut(s) 328, 397, 623, 952, 1106, 1410, 1484, 1496
Alw21I GWGCWC 4 cut(s) 330, 1072, 1467, 1532
AlwI GGATC 2 cut(s) 277, 1089
Ama87I CYCGRG 1 cut(s) 1491
AoxI GGCC 6 cut(s) 187, 461, 696, 758, 1263, 1404
ApeKI GCWGC 6 cut(s) 42, 210, 278, 406, 865, 952
ArsI GACNNNNNNTTYG 3 cut(s) 31, 884, 916
Asp700I GAANNNNTTC 4 cut(s) 248, 1171, 1514, 1702
AspLEI GCGC 4 cut(s) 361, 446, 745, 1281
AspS9I GGNCC 1 cut(s) 697
AsuC2I CCSGG 2 cut(s) 191, 1525
AsuHPI GGTGA 3 cut(s) 17, 1747, 1750
AvaI CYCGRG 1 cut(s) 1491
BaeI ACNNNNGTAYC 2 cut(s) 1277, 1310
BalI TGGCCA 1 cut(s) 1406
BanI GGYRCC 1 cut(s) 1441
BanII GRGCYC 2 cut(s) 330, 530
BbsI GAAGAC 1 cut(s) 1164
Bbv12I GWGCWC 4 cut(s) 330, 1072, 1467, 1532
BbvI GCAGC 6 cut(s) 54, 197, 290, 393, 852, 939
BccI CCATC 4 cut(s) 1088, 1367, 1417, 1769
BceAI ACGGC 4 cut(s) 353, 1278, 1458, 1573
BcgI CGANNNNNNTGC 6 cut(s) 267, 301, 884, 918, 1361, 1395
BciT130I CCWGG 5 cut(s) 740, 1132, 1588, 1643, 1658
BciVI GTATCC 2 cut(s) 813, 1351
BcnI CCSGG 2 cut(s) 191, 1525
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 4 cut(s) 122, 551, 783, 789
BfoI RGCGCY 1 cut(s) 746
BfuI GTATCC 2 cut(s) 813, 1351
BmcAI AGTACT 3 cut(s) 787, 1539, 1650
Bme1390I CCNGG 7 cut(s) 191, 740, 1132, 1525, 1588, 1643, 1658
BmeT110I CYCGRG 1 cut(s) 1491
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 4 cut(s) 306, 1035, 1227, 1443
BmrFI CCNGG 7 cut(s) 191, 740, 1132, 1525, 1588, 1643, 1658
BmsI GCATC 4 cut(s) 154, 553, 922, 1369
BpiI GAAGAC 1 cut(s) 1164
BpmI CTGGAG 1 cut(s) 1625
Bpu10I CCTNAGC 2 cut(s) 398, 1469
BpuEI CTTGAG 3 cut(s) 581, 710, 1319
BpuMI CCSGG 2 cut(s) 191, 1525
Bsa29I ATCGAT 3 cut(s) 128, 1371, 1724
BsaBI GATNNNNATC 1 cut(s) 1760
BsaHI GRCGYC 2 cut(s) 600, 672
BsaJI CCNNGG 6 cut(s) 333, 992, 1131, 1335, 1344, 1523
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 4 cut(s) 375, 466, 1235, 1531
Bse118I RCCGGY 4 cut(s) 185, 459, 463, 1265
Bse1I ACTGG 2 cut(s) 357, 591
Bse3DI GCAATG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 1760
BseBI CCWGG 5 cut(s) 740, 1132, 1588, 1643, 1658
BseCI ATCGAT 3 cut(s) 128, 1371, 1724
BseDI CCNNGG 6 cut(s) 333, 992, 1131, 1335, 1344, 1523
BseGI GGATG 3 cut(s) 847, 1099, 1715
BseJI GATNNNNATC 1 cut(s) 1760
BseLI CCNNNNNNNGG 4 cut(s) 375, 466, 1235, 1531
BseMI GCAATG 1 cut(s) 57
BseMII CTCAG 2 cut(s) 412, 1427
BseNI ACTGG 2 cut(s) 357, 591
BseRI GAGGAG 4 cut(s) 241, 297, 353, 1694
BseX3I CGGCCG 3 cut(s) 187, 461, 1263
BseXI GCAGC 6 cut(s) 54, 197, 290, 393, 852, 939
BsgI GTGCAG 2 cut(s) 893, 1130
Bsh1236I CGCG 1 cut(s) 359
Bsh1285I CGRYCG 4 cut(s) 190, 464, 1266, 1690
BshFI GGCC 6 cut(s) 189, 463, 698, 760, 1265, 1406
BshNI GGYRCC 1 cut(s) 1441
BshVI ATCGAT 3 cut(s) 128, 1371, 1724
BsiEI CGRYCG 4 cut(s) 190, 464, 1266, 1690
BsiHKAI GWGCWC 4 cut(s) 330, 1072, 1467, 1532
BsiHKCI CYCGRG 1 cut(s) 1491
BsiSI CCGG 6 cut(s) 186, 190, 460, 464, 1266, 1525
BslFI GGGAC 2 cut(s) 63, 1036
BslI CCNNNNNNNGG 4 cut(s) 375, 466, 1235, 1531
BsmFI GGGAC 2 cut(s) 63, 1036
BsmI GAATGC 2 cut(s) 867, 1661
BsnI GGCC 6 cut(s) 189, 463, 698, 760, 1265, 1406
BsoBI CYCGRG 1 cut(s) 1491
Bsp1286I GDGCHC 5 cut(s) 330, 530, 1072, 1467, 1532
Bsp1407I TGTACA 1 cut(s) 384
Bsp19I CCATGG 1 cut(s) 1335
BspANI GGCC 6 cut(s) 189, 463, 698, 760, 1265, 1406
BspCNI CTCAG 2 cut(s) 411, 1426
BspDI ATCGAT 3 cut(s) 128, 1371, 1724
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 4 cut(s) 306, 1035, 1227, 1443
BspPI GGATC 2 cut(s) 277, 1089
BspT107I GGYRCC 1 cut(s) 1441
BsrDI GCAATG 1 cut(s) 57
BsrFI RCCGGY 4 cut(s) 185, 459, 463, 1265
BsrGI TGTACA 1 cut(s) 384
BsrI ACTGG 2 cut(s) 357, 591
BssAI RCCGGY 4 cut(s) 185, 459, 463, 1265
BssECI CCNNGG 6 cut(s) 333, 992, 1131, 1335, 1344, 1523
BssNI GRCGYC 2 cut(s) 600, 672
BssT1I CCWWGG 3 cut(s) 333, 1335, 1344
Bst2UI CCWGG 5 cut(s) 740, 1132, 1588, 1643, 1658
Bst4CI ACNGT 4 cut(s) 77, 768, 1006, 1230
BstACI GRCGYC 2 cut(s) 600, 672
BstAPI GCANNNNNTGC 1 cut(s) 624
BstAUI TGTACA 1 cut(s) 384
BstDEI CTNAG 6 cut(s) 398, 1038, 1119, 1413, 1469, 1652
BstDSI CCRYGG 1 cut(s) 1335
BstF5I GGATG 3 cut(s) 847, 1099, 1715
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 1 cut(s) 746
BstHHI GCGC 4 cut(s) 361, 446, 745, 1281
BstMCI CGRYCG 4 cut(s) 190, 464, 1266, 1690
BstMWI GCNNNNNNNGC 6 cut(s) 365, 403, 452, 624, 865, 1606
BstNI CCWGG 5 cut(s) 740, 1132, 1588, 1643, 1658
BstNSI RCATGY 3 cut(s) 452, 613, 1016
BstSCI CCNGG 7 cut(s) 189, 738, 1130, 1523, 1586, 1641, 1656
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 6 cut(s) 54, 197, 290, 393, 852, 939
BstV2I GAAGAC 1 cut(s) 1164
BstX2I RGATCY 1 cut(s) 1081
BstXI CCANNNNNNTGG 2 cut(s) 1138, 1373
BstYI RGATCY 1 cut(s) 1081
BstZI CGGCCG 3 cut(s) 187, 461, 1263
Bsu15I ATCGAT 3 cut(s) 128, 1371, 1724
BsuI GTATCC 2 cut(s) 813, 1351
BsuRI GGCC 6 cut(s) 189, 463, 698, 760, 1265, 1406
BsuTUI ATCGAT 3 cut(s) 128, 1371, 1724
BtgI CCRYGG 1 cut(s) 1335
BtgZI GCGATG 5 cut(s) 945, 1037, 1284, 1392, 1575
BtsCI GGATG 3 cut(s) 847, 1099, 1715
BtsI GCAGTG 1 cut(s) 905
BtsIMutI CAGTG 2 cut(s) 905, 1002
CfoI GCGC 4 cut(s) 361, 446, 745, 1281
Cfr10I RCCGGY 4 cut(s) 185, 459, 463, 1265
Cfr13I GGNCC 1 cut(s) 697
ClaI ATCGAT 3 cut(s) 128, 1371, 1724
CseI GACGC 5 cut(s) 270, 365, 412, 446, 680
Csp6I GTAC 9 cut(s) 65, 385, 786, 1000, 1294, 1538, 1630, 1649, 1752
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 8 cut(s) 449, 478, 610, 1013, 1282, 1336, 1552, 1633
CviQI GTAC 9 cut(s) 65, 385, 786, 1000, 1294, 1538, 1630, 1649, 1752
DdeI CTNAG 6 cut(s) 398, 1038, 1119, 1413, 1469, 1652
DraIII CACNNNGTG 1 cut(s) 715
EaeI YGGCCR 4 cut(s) 187, 461, 1263, 1404
EagI CGGCCG 3 cut(s) 187, 461, 1263
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 328
EclXI CGGCCG 3 cut(s) 187, 461, 1263
Eco130I CCWWGG 3 cut(s) 333, 1335, 1344
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 2 cut(s) 330, 530
Eco32I GATATC 1 cut(s) 1325
Eco52I CGGCCG 3 cut(s) 187, 461, 1263
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 1491
EcoICRI GAGCTC 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 697
EcoRII CCWGG 5 cut(s) 738, 1130, 1586, 1641, 1656
EcoRV GATATC 1 cut(s) 1325
EcoT14I CCWWGG 3 cut(s) 333, 1335, 1344
EcoT38I GRGCYC 2 cut(s) 330, 530
ErhI CCWWGG 3 cut(s) 333, 1335, 1344
FaeI CATG 8 cut(s) 452, 481, 613, 1016, 1285, 1339, 1555, 1636
FalI AAGNNNNNCTT 4 cut(s) 1233, 1265, 1452, 1484
FaqI GGGAC 2 cut(s) 63, 1036
FatI CATG 8 cut(s) 448, 477, 609, 1012, 1281, 1335, 1551, 1632
FauI CCCGC 3 cut(s) 383, 631, 743
FauNDI CATATG 1 cut(s) 973
FblI GTMKAC 2 cut(s) 135, 1477
FokI GGATG 3 cut(s) 854, 1106, 1702
FriOI GRGCYC 2 cut(s) 330, 530
FspBI CTAG 4 cut(s) 122, 551, 783, 789
FspI TGCGCA 2 cut(s) 445, 1280
GlaI GCGC 4 cut(s) 360, 445, 744, 1280
GsuI CTGGAG 1 cut(s) 1625
HaeII RGCGCY 1 cut(s) 746
HaeIII GGCC 6 cut(s) 189, 463, 698, 760, 1265, 1406
HapII CCGG 6 cut(s) 186, 190, 460, 464, 1266, 1525
HgaI GACGC 5 cut(s) 270, 365, 412, 446, 680
HhaI GCGC 4 cut(s) 361, 446, 745, 1281
Hin1I GRCGYC 2 cut(s) 600, 672
Hin1II CATG 8 cut(s) 452, 481, 613, 1016, 1285, 1339, 1555, 1636
Hin6I GCGC 4 cut(s) 359, 444, 743, 1279
HinP1I GCGC 4 cut(s) 359, 444, 743, 1279
HincII GTYRAC 2 cut(s) 136, 181
HindII GTYRAC 2 cut(s) 136, 181
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 6 cut(s) 125, 137, 731, 836, 988, 1576
HpaII CCGG 6 cut(s) 186, 190, 460, 464, 1266, 1525
HphI GGTGA 3 cut(s) 17, 1747, 1750
Hpy166II GTNNAC 7 cut(s) 136, 181, 205, 433, 1002, 1429, 1478
Hpy8I GTNNAC 7 cut(s) 136, 181, 205, 433, 1002, 1429, 1478
Hpy99I CGWCG 9 cut(s) 179, 286, 289, 298, 440, 605, 674, 680, 896
HpyCH4III ACNGT 4 cut(s) 77, 768, 1006, 1230
HpyCH4IV ACGT 3 cut(s) 246, 600, 678
HpyF10VI GCNNNNNNNGC 6 cut(s) 365, 403, 452, 624, 865, 1606
HpyF3I CTNAG 6 cut(s) 398, 1038, 1119, 1413, 1469, 1652
HpySE526I ACGT 3 cut(s) 246, 600, 678
Hsp92I GRCGYC 2 cut(s) 600, 672
Hsp92II CATG 8 cut(s) 452, 481, 613, 1016, 1285, 1339, 1555, 1636
HspAI GCGC 4 cut(s) 359, 444, 743, 1279
KroI GCCGGC 3 cut(s) 185, 459, 1265
KroNI GCCGGC 3 cut(s) 187, 461, 1267
LmnI GCTCC 7 cut(s) 228, 310, 325, 366, 949, 1033, 1207
Lsp1109I GCAGC 6 cut(s) 54, 197, 290, 393, 852, 939
LweI GCATC 4 cut(s) 154, 553, 922, 1369
MaeI CTAG 4 cut(s) 122, 551, 783, 789
MaeII ACGT 3 cut(s) 246, 600, 678
MaeIII GTNAC 1 cut(s) 5
MboII GAAGA 6 cut(s) 1164, 1167, 1630, 1695, 1698, 1701
MflI RGATCY 1 cut(s) 1081
MhlI GDGCHC 5 cut(s) 330, 530, 1072, 1467, 1532
MlsI TGGCCA 1 cut(s) 1406
MluCI AATT 4 cut(s) 317, 521, 966, 1604
MluNI TGGCCA 1 cut(s) 1406
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 5 cut(s) 60, 138, 328, 693, 1291
Mox20I TGGCCA 1 cut(s) 1406
MroNI GCCGGC 3 cut(s) 185, 459, 1265
MroXI GAANNNNTTC 4 cut(s) 248, 1171, 1514, 1702
MscI TGGCCA 1 cut(s) 1406
MseI TTAA 3 cut(s) 978, 1149, 1433
MslI CAYNNNNRTG 2 cut(s) 587, 777
Msp20I TGGCCA 1 cut(s) 1406
MspA1I CMGCKG 2 cut(s) 210, 390
MspI CCGG 6 cut(s) 186, 190, 460, 464, 1266, 1525
MspR9I CCNGG 7 cut(s) 191, 740, 1132, 1525, 1588, 1643, 1658
Mva1269I GAATGC 2 cut(s) 867, 1661
MvaI CCWGG 5 cut(s) 740, 1132, 1588, 1643, 1658
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 6 cut(s) 365, 403, 452, 624, 865, 1606
NaeI GCCGGC 3 cut(s) 187, 461, 1267
NciI CCSGG 2 cut(s) 191, 1525
NcoI CCATGG 1 cut(s) 1335
NdeI CATATG 1 cut(s) 973
NgoMIV GCCGGC 3 cut(s) 185, 459, 1265
NlaIII CATG 8 cut(s) 452, 481, 613, 1016, 1285, 1339, 1555, 1636
NlaIV GGNNCC 4 cut(s) 306, 1035, 1227, 1443
NmeAIII GCCGAG 2 cut(s) 233, 726
NmuCI GTSAC 1 cut(s) 5
NsbI TGCGCA 2 cut(s) 445, 1280
NspI RCATGY 3 cut(s) 452, 613, 1016
OliI CACNNNNGTG 1 cut(s) 777
PaeR7I CTCGAG 1 cut(s) 1491
PceI AGGCCT 1 cut(s) 760
PciI ACATGT 2 cut(s) 448, 609
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 290, 927
PctI GAATGC 2 cut(s) 867, 1661
PdiI GCCGGC 3 cut(s) 187, 461, 1267
PdmI GAANNNNTTC 4 cut(s) 248, 1171, 1514, 1702
PfeI GAWTC 5 cut(s) 125, 731, 836, 988, 1576
PfoI TCCNGGA 1 cut(s) 1641
Ple19I CGATCG 1 cut(s) 1690
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PscI ACATGT 2 cut(s) 448, 609
PsiI TTATAA 1 cut(s) 683
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 5 cut(s) 738, 1130, 1586, 1641, 1656
PspGI CCWGG 5 cut(s) 738, 1130, 1586, 1641, 1656
PspN4I GGNNCC 4 cut(s) 306, 1035, 1227, 1443
PspPI GGNCC 1 cut(s) 697
PsuI RGATCY 1 cut(s) 1081
PvuI CGATCG 1 cut(s) 1690
RsaI GTAC 9 cut(s) 66, 386, 787, 1001, 1295, 1539, 1631, 1650, 1753
RsaNI GTAC 9 cut(s) 65, 385, 786, 1000, 1294, 1538, 1630, 1649, 1752
RseI CAYNNNNRTG 2 cut(s) 587, 777
SacI GAGCTC 1 cut(s) 330
SalI GTCGAC 1 cut(s) 134
SaqAI TTAA 3 cut(s) 978, 1149, 1433
Sau96I GGNCC 1 cut(s) 697
ScaI AGTACT 3 cut(s) 787, 1539, 1650
SchI GAGTC 1 cut(s) 131
ScrFI CCNGG 7 cut(s) 191, 740, 1132, 1525, 1588, 1643, 1658
SduI GDGCHC 5 cut(s) 330, 530, 1072, 1467, 1532
SfaNI GCATC 4 cut(s) 154, 553, 922, 1369
Sfr274I CTCGAG 1 cut(s) 1491
SlaI CTCGAG 1 cut(s) 1491
SmiMI CAYNNNNRTG 2 cut(s) 587, 777
SmlI CTYRAG 4 cut(s) 560, 689, 1298, 1491
SmoI CTYRAG 4 cut(s) 560, 689, 1298, 1491
SpeI ACTAGT 1 cut(s) 788
Sse9I AATT 4 cut(s) 317, 521, 966, 1604
SseBI AGGCCT 1 cut(s) 760
SspMI CTAG 4 cut(s) 122, 551, 783, 789
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 7 cut(s) 189, 738, 1130, 1523, 1586, 1641, 1656
StyI CCWWGG 3 cut(s) 333, 1335, 1344
TaaI ACNGT 4 cut(s) 77, 768, 1006, 1230
TaiI ACGT 3 cut(s) 249, 603, 681
TaqII GACCGA 1 cut(s) 1680
TasI AATT 4 cut(s) 317, 521, 966, 1604
TatI WGTACW 4 cut(s) 384, 785, 1537, 1648
TauI GCSGC 6 cut(s) 305, 618, 706, 1022, 1126, 1612
TfiI GAWTC 5 cut(s) 125, 731, 836, 988, 1576
Tru1I TTAA 3 cut(s) 978, 1149, 1433
Tru9I TTAA 3 cut(s) 978, 1149, 1433
TscAI CASTG 2 cut(s) 912, 1009
TseFI GTSAC 1 cut(s) 5
TseI GCWGC 6 cut(s) 42, 210, 278, 406, 865, 952
Tsp45I GTSAC 1 cut(s) 5
TspDTI ATGAA 2 cut(s) 466, 1298
TspGWI ACGGA 2 cut(s) 703, 962
TspRI CASTG 2 cut(s) 912, 1009
XceI RCATGY 3 cut(s) 452, 613, 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 1351
XhoI CTCGAG 1 cut(s) 1491
XmiI GTMKAC 2 cut(s) 135, 1477
XmnI GAANNNNTTC 4 cut(s) 248, 1171, 1514, 1702
XspI CTAG 4 cut(s) 122, 551, 783, 789
ZraI GACGTC 1 cut(s) 601
ZrmI AGTACT 3 cut(s) 787, 1539, 1650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.