pycom02g11670

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011777
Physical Location & Seq
Forward (+)
8234330 .. 8236266
1937 bp
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UTR
Exon/CDS
Intron
pycom02g11670.1

Sequence Viewer

Length: 468 bp
ATGCCAACCCCGTGGTCCACGCTAAGAAGGAAAGGGTTGCTCGCACCGAAGATCTCCACGCCGACGACGCCGATCGATCCTCTCGATATCTATGATTTCGTGAGGGATATTAGAGATCCGGAGCACCCCTACTCGTTAGAGCAGCTCAGCGTGCTTTCGGAGGAGTCGATCACTGTGGATGACAAGCTCGGCCGTATTCTGATAACTTTTACGCCGACGATTCAGCATTGCAGTATGGCAACAGTGATAGGTCTGTGCCTGAGACTTAAACTGAAACATTGCTTCCCTCCTCATTACAAGGTCCGTCAGTTTGCTATGGCGGTTGATATTAAAGTATCTCCAGGATCTCATGCAGATGAAGAATCAGTTAACAAGCAGTTGAATGATAAAGAAAGAGTTGCTGCTGCCTTGGAGAACCCCAACCTTCGCCAACTTGTGGACGAGTGCCTGTATTCCAACGAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

156

Amino Acids

17.57

Weight (kDa)

5.94

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011777)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50845
fragaria_vesca FvH4_1g13320 FvH4_1g13320 FvH4_1g13320
malus_domestica MD02G1148000.v1.1 MD11G1309300.v1.1
prunus_persica Prupe.7G154400_v2.0.a1 Prupe.7G154400_v2.0.a1
pyrus_communis pycom02g11670
rosa_chinensis RchiOBHm_Chr2g0101601
rosa_laevigata RLG00000017093
rosa_multiflora Rmu_sc0006153.1_g000005
rosa_roxburghii Rroxscaffold_2G00141160
rosa_rugosa Rorug02G0101000
rosa_samantha Rh2AG148300 Rh2BG153700 Rh2CG154100 Rh2DG154100
rosa_wichuraiana Rw2G011570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 436
AccIII TCCGGA 1 cut(s) 118
AciI CCGC 1 cut(s) 320
AclWI GGATC 3 cut(s) 71, 110, 352
AcoI YGGCCR 1 cut(s) 190
AcyI GRCGYC 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 436
AgsI TTSAA 1 cut(s) 382
AjnI CCWGG 1 cut(s) 340
AluBI AGCT 2 cut(s) 145, 187
AluI AGCT 2 cut(s) 145, 187
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 3 cut(s) 71, 110, 352
Aor13HI TCCGGA 1 cut(s) 118
AoxI GGCC 1 cut(s) 190
ApeKI GCWGC 3 cut(s) 142, 401, 404
AspS9I GGNCC 2 cut(s) 15, 301
AvaII GGWCC 2 cut(s) 15, 301
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 3 cut(s) 154, 388, 391
BceAI ACGGC 1 cut(s) 177
BciT130I CCWGG 1 cut(s) 342
BcoDI GTCTC 1 cut(s) 256
BglII AGATCT 1 cut(s) 51
BisI GCNGC 3 cut(s) 143, 402, 405
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 3 cut(s) 144, 403, 406
Bme1390I CCNGG 1 cut(s) 342
Bme18I GGWCC 2 cut(s) 15, 301
BmgT120I GGNCC 2 cut(s) 15, 301
BmrFI CCNGG 1 cut(s) 342
BpmI CTGGAG 1 cut(s) 324
Bpu1102I GCTNAGC 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 75
BsaHI GRCGYC 1 cut(s) 68
BsaJI CCNNGG 2 cut(s) 11, 408
BsaWI WCCGGW 1 cut(s) 118
Bsc4I CCNNNNNNNGG 1 cut(s) 436
Bse3DI GCAATG 2 cut(s) 226, 277
BseAI TCCGGA 1 cut(s) 118
BseBI CCWGG 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 2 cut(s) 11, 408
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 436
BseMI GCAATG 2 cut(s) 226, 277
BseMII CTCAG 2 cut(s) 160, 251
BseRI GAGGAG 2 cut(s) 176, 279
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 3 cut(s) 154, 388, 391
Bsh1285I CGRYCG 2 cut(s) 75, 193
BshFI GGCC 1 cut(s) 192
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 2 cut(s) 75, 193
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 436
BsmAI GTCTC 1 cut(s) 256
BsnI GGCC 1 cut(s) 192
Bsp1286I GDGCHC 1 cut(s) 126
Bsp13I TCCGGA 1 cut(s) 118
Bsp143I GATC 6 cut(s) 51, 72, 76, 115, 168, 344
Bsp1720I GCTNAGC 1 cut(s) 146
BspACI CCGC 1 cut(s) 320
BspANI GGCC 1 cut(s) 192
BspCNI CTCAG 2 cut(s) 159, 252
BspDI ATCGAT 1 cut(s) 75
BspEI TCCGGA 1 cut(s) 118
BspPI GGATC 3 cut(s) 71, 110, 352
BsrDI GCAATG 2 cut(s) 226, 277
BssECI CCNNGG 2 cut(s) 11, 408
BssMI GATC 6 cut(s) 51, 72, 76, 115, 168, 344
BssNI GRCGYC 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 175, 244
BstACI GRCGYC 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 42, 152
BstDEI CTNAG 3 cut(s) 23, 146, 260
BstDSI CCRYGG 1 cut(s) 11
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 6 cut(s) 54, 75, 79, 118, 171, 347
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 6 cut(s) 51, 72, 76, 115, 168, 344
BstMCI CGRYCG 2 cut(s) 75, 193
BstMWI GCNNNNNNNGC 2 cut(s) 67, 151
BstNI CCWGG 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 340
BstV1I GCAGC 3 cut(s) 154, 388, 391
BstX2I RGATCY 3 cut(s) 51, 115, 344
BstXI CCANNNNNNTGG 1 cut(s) 12
BstYI RGATCY 3 cut(s) 51, 115, 344
BstZI CGGCCG 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 75
BsuRI GGCC 1 cut(s) 192
BsuTUI ATCGAT 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 11
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 171, 249
Cac8I GCNNGC 2 cut(s) 42, 152
Cfr13I GGNCC 2 cut(s) 15, 301
ClaI ATCGAT 1 cut(s) 75
CseI GACGC 1 cut(s) 76
CviAII CATG 1 cut(s) 350
CviJI RGCY 3 cut(s) 145, 187, 192
CviKI_1 RGCY 3 cut(s) 145, 187, 192
DdeI CTNAG 3 cut(s) 23, 146, 260
DpnI GATC 6 cut(s) 53, 74, 78, 117, 170, 346
DpnII GATC 6 cut(s) 51, 72, 76, 115, 168, 344
EaeI YGGCCR 1 cut(s) 190
EagI CGGCCG 1 cut(s) 190
EclXI CGGCCG 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 408
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 2 cut(s) 15, 301
Eco52I CGGCCG 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 340
EcoRV GATATC 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 1 cut(s) 353
FaiI YATR 4 cut(s) 93, 236, 317, 351
FatI CATG 1 cut(s) 349
Fnu4HI GCNGC 3 cut(s) 143, 402, 405
FokI GGATG 1 cut(s) 191
Fsp4HI GCNGC 3 cut(s) 143, 402, 405
GluI GCNGC 3 cut(s) 143, 402, 405
GsuI CTGGAG 1 cut(s) 324
HaeIII GGCC 1 cut(s) 192
HapII CCGG 1 cut(s) 119
HgaI GACGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 68
Hin1II CATG 1 cut(s) 353
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 3 cut(s) 164, 220, 362
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 1 cut(s) 119
Hpy166II GTNNAC 3 cut(s) 18, 370, 439
Hpy188I TCNGA 3 cut(s) 160, 201, 467
Hpy188III TCNNGA 3 cut(s) 83, 100, 119
Hpy8I GTNNAC 3 cut(s) 18, 370, 439
Hpy99I CGWCG 3 cut(s) 67, 70, 220
HpyAV CCTTC 2 cut(s) 21, 434
HpyCH4III ACNGT 2 cut(s) 175, 244
HpyCH4V TGCA 2 cut(s) 231, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 151
HpyF3I CTNAG 3 cut(s) 23, 146, 260
Hsp92I GRCGYC 1 cut(s) 68
Hsp92II CATG 1 cut(s) 353
Kpn2I TCCGGA 1 cut(s) 118
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 6 cut(s) 51, 72, 76, 115, 168, 344
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 5 cut(s) 132, 272, 327, 354, 461
Lsp1109I GCAGC 3 cut(s) 154, 388, 391
MalI GATC 6 cut(s) 53, 74, 78, 117, 170, 346
MboI GATC 6 cut(s) 51, 72, 76, 115, 168, 344
MboII GAAGA 2 cut(s) 61, 371
MflI RGATCY 3 cut(s) 51, 115, 344
MhlI GDGCHC 1 cut(s) 126
MlyI GAGTC 1 cut(s) 173
MnlI CCTC 5 cut(s) 90, 96, 154, 297, 300
MroI TCCGGA 1 cut(s) 118
MseI TTAA 3 cut(s) 267, 330, 369
MslI CAYNNNNRTG 1 cut(s) 354
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 342
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 67, 151
NdeII GATC 6 cut(s) 51, 72, 76, 115, 168, 344
NlaIII CATG 1 cut(s) 353
NmeAIII GCCGAG 1 cut(s) 168
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 164
PfeI GAWTC 2 cut(s) 220, 362
PflMI CCANNNNNTGG 1 cut(s) 436
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 3 cut(s) 144, 403, 406
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 340
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 2 cut(s) 15, 301
PsuI RGATCY 3 cut(s) 51, 115, 344
PvuI CGATCG 1 cut(s) 75
RseI CAYNNNNRTG 1 cut(s) 354
SaqAI TTAA 3 cut(s) 267, 330, 369
SatI GCNGC 3 cut(s) 143, 402, 405
Sau3AI GATC 6 cut(s) 51, 72, 76, 115, 168, 344
Sau96I GGNCC 2 cut(s) 15, 301
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 1 cut(s) 342
SduI GDGCHC 1 cut(s) 126
SetI ASST 5 cut(s) 147, 189, 253, 303, 426
SinI GGWCC 2 cut(s) 15, 301
SmiMI CAYNNNNRTG 1 cut(s) 354
SsiI CCGC 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 340
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 2 cut(s) 175, 244
TaqI TCGA 3 cut(s) 75, 84, 167
TfiI GAWTC 2 cut(s) 220, 362
Tru1I TTAA 3 cut(s) 267, 330, 369
Tru9I TTAA 3 cut(s) 267, 330, 369
TscAI CASTG 2 cut(s) 178, 249
TseI GCWGC 3 cut(s) 142, 401, 404
TspDTI ATGAA 1 cut(s) 372
TspGWI ACGGA 1 cut(s) 293
TspRI CASTG 2 cut(s) 178, 249
Van91I CCANNNNNTGG 1 cut(s) 436
VpaK11BI GGWCC 2 cut(s) 15, 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.