Rroxscaffold_2G00141160

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011777
Physical Location & Seq
Reverse (-)
79480275 .. 79482430
2156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141160.1

Sequence Viewer

Length: 471 bp
ATGACGCTGGGTCTGATCAATGCAAACCCGGTGGTGCACGCTAAGAAGGAAAGGGTTGCCCGCACCGAAGATCTTCACTCCGACGTCGACGCCGCCGTTGATCCTCTCGAAATCTATGATTTCGTGAGGGATATAAGAGATCCGGAGCATCCGTACTCGTTGGAGCAGCTCAGCGTGCTTTCCGAGGAGTCCATCACCGTCGACGACAAGCTCGGCCGGATTCTGATTACGTTCACGCCGACCATTCAGCATTGCAGTATGGCAACAGTGATAGGACTATGCCTGAGAGTTAAACTCAAGCAATGTTTCCCTCCTCATTACAAGGTTGACATTAAGGTATCTCCAGGATCCCATGCAGATGAAGAATCAGTTAATAAGCAGTTGAATGATAAAGAAAGAGTTGCTGCTGCCCTGGAGAATCCTAACCTTCGCCAACTTGTGGATGAGTGCCTTTATTCCAGTGAACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

156

Amino Acids

17.53

Weight (kDa)

5.07

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 37 - 100 5.7e-06 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011777)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50845
fragaria_vesca FvH4_1g13320 FvH4_1g13320 FvH4_1g13320
malus_domestica MD02G1148000.v1.1 MD11G1309300.v1.1
prunus_persica Prupe.7G154400_v2.0.a1 Prupe.7G154400_v2.0.a1
pyrus_communis pycom02g11670
rosa_chinensis RchiOBHm_Chr2g0101601
rosa_laevigata RLG00000017093
rosa_multiflora Rmu_sc0006153.1_g000005
rosa_roxburghii Rroxscaffold_2G00141160
rosa_rugosa Rorug02G0101000
rosa_samantha Rh2AG148300 Rh2BG153700 Rh2CG154100 Rh2DG154100
rosa_wichuraiana Rw2G011570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
AccB7I CCANNNNNTGG 1 cut(s) 439
AccI GTMKAC 2 cut(s) 87, 201
AccIII TCCGGA 1 cut(s) 142
AciI CCGC 2 cut(s) 61, 93
AclWI GGATC 4 cut(s) 95, 134, 342, 355
AcoI YGGCCR 1 cut(s) 214
AcyI GRCGYC 2 cut(s) 84, 90
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 1 cut(s) 439
AgsI TTSAA 1 cut(s) 385
AhdI GACNNNNNGTC 1 cut(s) 9
AjnI CCWGG 2 cut(s) 343, 411
AluBI AGCT 2 cut(s) 169, 211
AluI AGCT 2 cut(s) 169, 211
Alw21I GWGCWC 1 cut(s) 39
Alw44I GTGCAC 1 cut(s) 35
AlwI GGATC 4 cut(s) 95, 134, 342, 355
Aor13HI TCCGGA 1 cut(s) 142
AoxI GGCC 1 cut(s) 214
ApaLI GTGCAC 1 cut(s) 35
ApeKI GCWGC 3 cut(s) 166, 404, 407
Asp700I GAANNNNTTC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 39
BamHI GGATCC 1 cut(s) 347
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 178, 391, 394
BccI CCATC 1 cut(s) 200
BceAI ACGGC 1 cut(s) 80
BciT130I CCWGG 2 cut(s) 345, 413
BclI TGATCA 1 cut(s) 15
BcnI CCSGG 1 cut(s) 29
BglII AGATCT 1 cut(s) 70
BisI GCNGC 4 cut(s) 93, 167, 405, 408
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 4 cut(s) 94, 168, 406, 409
Bme1390I CCNGG 3 cut(s) 29, 345, 413
BmeRI GACNNNNNGTC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 3 cut(s) 29, 345, 413
BmsI GCATC 1 cut(s) 157
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BpmI CTGGAG 2 cut(s) 327, 434
Bpu1102I GCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 29
BsaHI GRCGYC 2 cut(s) 84, 90
BsaJI CCNNGG 2 cut(s) 183, 411
BsaWI WCCGGW 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse1I ACTGG 1 cut(s) 459
Bse3DI GCAATG 2 cut(s) 250, 308
BseAI TCCGGA 1 cut(s) 142
BseBI CCWGG 2 cut(s) 345, 413
BseDI CCNNGG 2 cut(s) 183, 411
BseGI GGATG 2 cut(s) 148, 448
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 2 cut(s) 250, 308
BseMII CTCAG 2 cut(s) 184, 275
BseNI ACTGG 1 cut(s) 459
BseRI GAGGAG 2 cut(s) 200, 303
BseSI GKGCMC 1 cut(s) 39
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 3 cut(s) 178, 391, 394
BseYI CCCAGC 1 cut(s) 7
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 216
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 3 cut(s) 29, 143, 217
BslI CCNNNNNNNGG 1 cut(s) 439
BsnI GGCC 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 39
Bsp13I TCCGGA 1 cut(s) 142
Bsp143I GATC 5 cut(s) 15, 70, 100, 139, 347
Bsp1720I GCTNAGC 1 cut(s) 170
BspACI CCGC 2 cut(s) 61, 93
BspANI GGCC 1 cut(s) 216
BspCNI CTCAG 2 cut(s) 183, 276
BspEI TCCGGA 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 349
BspPI GGATC 4 cut(s) 95, 134, 342, 355
BsrDI GCAATG 2 cut(s) 250, 308
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 2 cut(s) 183, 411
BssMI GATC 5 cut(s) 15, 70, 100, 139, 347
BssNI GRCGYC 2 cut(s) 84, 90
Bst2UI CCWGG 2 cut(s) 345, 413
Bst4CI ACNGT 3 cut(s) 199, 268, 468
BstACI GRCGYC 2 cut(s) 84, 90
BstC8I GCNNGC 3 cut(s) 39, 61, 176
BstDEI CTNAG 3 cut(s) 42, 170, 284
BstF5I GGATG 2 cut(s) 148, 448
BstKTI GATC 5 cut(s) 18, 73, 103, 142, 350
BstMBI GATC 5 cut(s) 15, 70, 100, 139, 347
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNI CCWGG 2 cut(s) 345, 413
BstSCI CCNGG 3 cut(s) 27, 343, 411
BstSLI GKGCMC 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 178, 391, 394
BstX2I RGATCY 3 cut(s) 70, 139, 347
BstYI RGATCY 3 cut(s) 70, 139, 347
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 1 cut(s) 216
BtsCI GGATG 2 cut(s) 148, 448
BtsIMutI CAGTG 2 cut(s) 273, 466
Cac8I GCNNGC 3 cut(s) 39, 61, 176
CseI GACGC 2 cut(s) 13, 98
Csp6I GTAC 1 cut(s) 154
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 1 cut(s) 353
CviJI RGCY 3 cut(s) 169, 211, 216
CviKI_1 RGCY 3 cut(s) 169, 211, 216
CviQI GTAC 1 cut(s) 154
DdeI CTNAG 3 cut(s) 42, 170, 284
DpnI GATC 5 cut(s) 17, 72, 102, 141, 349
DpnII GATC 5 cut(s) 15, 70, 100, 139, 347
DriI GACNNNNNGTC 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Eam1105I GACNNNNNGTC 1 cut(s) 9
EclXI CGGCCG 1 cut(s) 214
Eco52I CGGCCG 1 cut(s) 214
EcoRII CCWGG 2 cut(s) 343, 411
FaeI CATG 1 cut(s) 356
FaiI YATR 5 cut(s) 117, 134, 260, 280, 354
FatI CATG 1 cut(s) 352
FauI CCCGC 1 cut(s) 68
FbaI TGATCA 1 cut(s) 15
FblI GTMKAC 2 cut(s) 87, 201
Fnu4HI GCNGC 4 cut(s) 93, 167, 405, 408
FokI GGATG 2 cut(s) 135, 455
Fsp4HI GCNGC 4 cut(s) 93, 167, 405, 408
GluI GCNGC 4 cut(s) 93, 167, 405, 408
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 2 cut(s) 327, 434
HaeIII GGCC 1 cut(s) 216
HapII CCGG 3 cut(s) 29, 143, 217
HgaI GACGC 2 cut(s) 13, 98
Hin1I GRCGYC 2 cut(s) 84, 90
Hin1II CATG 1 cut(s) 356
HincII GTYRAC 3 cut(s) 88, 202, 328
HindII GTYRAC 3 cut(s) 88, 202, 328
HinfI GANTC 4 cut(s) 188, 220, 365, 418
HpaII CCGG 3 cut(s) 29, 143, 217
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 6 cut(s) 37, 88, 202, 234, 328, 464
Hpy188I TCNGA 4 cut(s) 15, 82, 184, 225
Hpy188III TCNNGA 3 cut(s) 107, 124, 143
Hpy8I GTNNAC 6 cut(s) 37, 88, 202, 234, 328, 464
Hpy99I CGWCG 5 cut(s) 86, 89, 92, 203, 206
HpyAV CCTTC 2 cut(s) 40, 437
HpyCH4III ACNGT 3 cut(s) 199, 268, 468
HpyCH4IV ACGT 2 cut(s) 84, 230
HpyCH4V TGCA 4 cut(s) 23, 37, 255, 356
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 3 cut(s) 42, 170, 284
HpySE526I ACGT 2 cut(s) 84, 230
Hsp92I GRCGYC 2 cut(s) 84, 90
Hsp92II CATG 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 15
Kzo9I GATC 5 cut(s) 15, 70, 100, 139, 347
LmnI GCTCC 2 cut(s) 145, 163
LpnPI CCDG 8 cut(s) 42, 156, 230, 296, 330, 357, 398, 425
Lsp1109I GCAGC 3 cut(s) 178, 391, 394
LweI GCATC 1 cut(s) 157
MaeII ACGT 2 cut(s) 84, 230
MalI GATC 5 cut(s) 17, 72, 102, 141, 349
MboI GATC 5 cut(s) 15, 70, 100, 139, 347
MboII GAAGA 3 cut(s) 65, 80, 374
MflI RGATCY 3 cut(s) 70, 139, 347
MhlI GDGCHC 1 cut(s) 39
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 105, 141
MnlI CCTC 5 cut(s) 114, 120, 178, 321, 324
MroI TCCGGA 1 cut(s) 142
MroXI GAANNNNTTC 1 cut(s) 72
MseI TTAA 3 cut(s) 291, 333, 372
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 3 cut(s) 29, 143, 217
MspR9I CCNGG 3 cut(s) 29, 345, 413
MvaI CCWGG 2 cut(s) 345, 413
MwoI GCNNNNNNNGC 1 cut(s) 175
NciI CCSGG 1 cut(s) 29
NdeII GATC 5 cut(s) 15, 70, 100, 139, 347
NlaIII CATG 1 cut(s) 356
NlaIV GGNNCC 1 cut(s) 349
NmeAIII GCCGAG 1 cut(s) 192
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 236
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 3 cut(s) 220, 365, 418
PflMI CCANNNNNTGG 1 cut(s) 439
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 4 cut(s) 94, 168, 406, 409
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp6I CCWGG 2 cut(s) 343, 411
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 2 cut(s) 343, 411
PspN4I GGNNCC 1 cut(s) 349
PsuI RGATCY 3 cut(s) 70, 139, 347
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 357
SalI GTCGAC 2 cut(s) 86, 200
SaqAI TTAA 3 cut(s) 291, 333, 372
SatI GCNGC 4 cut(s) 93, 167, 405, 408
Sau3AI GATC 5 cut(s) 15, 70, 100, 139, 347
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 3 cut(s) 29, 345, 413
SduI GDGCHC 1 cut(s) 39
SetI ASST 7 cut(s) 87, 171, 213, 233, 327, 339, 429
SfaNI GCATC 1 cut(s) 157
SgrDI CGTCGACG 2 cut(s) 86, 200
SmiMI CAYNNNNRTG 1 cut(s) 357
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SsiI CCGC 2 cut(s) 61, 93
StyD4I CCNGG 3 cut(s) 27, 343, 411
TaaI ACNGT 3 cut(s) 199, 268, 468
TaiI ACGT 2 cut(s) 87, 233
TaqI TCGA 3 cut(s) 87, 108, 201
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 3 cut(s) 220, 365, 418
Tru1I TTAA 3 cut(s) 291, 333, 372
Tru9I TTAA 3 cut(s) 291, 333, 372
TscAI CASTG 2 cut(s) 273, 466
TseI GCWGC 3 cut(s) 166, 404, 407
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 2 cut(s) 273, 466
Van91I CCANNNNNTGG 1 cut(s) 439
VneI GTGCAC 1 cut(s) 35
XmiI GTMKAC 2 cut(s) 87, 201
XmnI GAANNNNTTC 1 cut(s) 72
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.