Rh2BG153700

MIP18 family protein At1g68310-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011777
Physical Location & Seq
Forward (+)
13161624 .. 13165721
4098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG153700.1

Sequence Viewer

Length: 303 bp
ATGGTAAATTTCGTGAGGGATATAAGAGATCCGGAGCATCCGTACTCGTTAGAGCAGCTCAGCGTGCTTTCCGAGGAGTCCATCACCGTCGACGACAAGCTCGGCCGGATTCTGATTACGTTCACGCCGACCATTCAGCATTGCAGTATGGCAACAGTGATAGGACTATGCCTGAGAGTTAAACTCAAGCAATGTTTCCCTCCTCATTACAAGGTTGACATTAAGGTATCTCCAGGATCTCATGCAGATGAAGAATCAGGTAAGGAATCTCCGTTCTCTTTTATAGCATCAGGTTATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FeS_assembly_P

Protein Analysis

100

Amino Acids

11.21

Weight (kDa)

5.47

Isoelectric Point (pI)

44.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeS_assembly_P PF01883 6 - 63 7.3e-06 Iron-sulfur cluster assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011777)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50845
fragaria_vesca FvH4_1g13320 FvH4_1g13320 FvH4_1g13320
malus_domestica MD02G1148000.v1.1 MD11G1309300.v1.1
prunus_persica Prupe.7G154400_v2.0.a1 Prupe.7G154400_v2.0.a1
pyrus_communis pycom02g11670
rosa_chinensis RchiOBHm_Chr2g0101601
rosa_laevigata RLG00000017093
rosa_multiflora Rmu_sc0006153.1_g000005
rosa_roxburghii Rroxscaffold_2G00141160
rosa_rugosa Rorug02G0101000
rosa_samantha Rh2AG148300 Rh2BG153700 Rh2CG154100 Rh2DG154100
rosa_wichuraiana Rw2G011570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 31
AclWI GGATC 2 cut(s) 23, 244
AcoI YGGCCR 1 cut(s) 103
AcsI RAATTY 1 cut(s) 7
AfaI GTAC 1 cut(s) 44
AjnI CCWGG 1 cut(s) 232
AloI GAACNNNNNNTCC 2 cut(s) 257, 289
AluBI AGCT 2 cut(s) 58, 100
AluI AGCT 2 cut(s) 58, 100
AlwI GGATC 2 cut(s) 23, 244
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 76
BbvI GCAGC 1 cut(s) 67
BccI CCATC 1 cut(s) 89
BciT130I CCWGG 1 cut(s) 234
BisI GCNGC 1 cut(s) 56
BlpI GCTNAGC 1 cut(s) 59
BlsI GCNGC 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 234
BmsI GCATC 2 cut(s) 46, 296
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 59
BpuEI CTTGAG 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 72
BsaWI WCCGGW 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bse3DI GCAATG 2 cut(s) 139, 197
BseAI TCCGGA 1 cut(s) 31
BseBI CCWGG 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 72
BseGI GGATG 1 cut(s) 37
BseMI GCAATG 2 cut(s) 139, 197
BseMII CTCAG 2 cut(s) 73, 164
BseRI GAGGAG 2 cut(s) 89, 192
BseX3I CGGCCG 1 cut(s) 103
BseXI GCAGC 1 cut(s) 67
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 1 cut(s) 105
BsiEI CGRYCG 1 cut(s) 106
BsiSI CCGG 2 cut(s) 32, 106
BsnI GGCC 1 cut(s) 105
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 2 cut(s) 28, 236
Bsp1720I GCTNAGC 1 cut(s) 59
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 2 cut(s) 72, 165
BspEI TCCGGA 1 cut(s) 31
BspPI GGATC 2 cut(s) 23, 244
BsrDI GCAATG 2 cut(s) 139, 197
BssECI CCNNGG 1 cut(s) 72
BssMI GATC 2 cut(s) 28, 236
Bst2UI CCWGG 1 cut(s) 234
Bst4CI ACNGT 2 cut(s) 88, 157
BstC8I GCNNGC 1 cut(s) 65
BstDEI CTNAG 2 cut(s) 59, 173
BstF5I GGATG 1 cut(s) 37
BstKTI GATC 2 cut(s) 31, 239
BstMBI GATC 2 cut(s) 28, 236
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 64
BstNI CCWGG 1 cut(s) 234
BstSCI CCNGG 1 cut(s) 232
BstV1I GCAGC 1 cut(s) 67
BstX2I RGATCY 2 cut(s) 28, 236
BstYI RGATCY 2 cut(s) 28, 236
BstZI CGGCCG 1 cut(s) 103
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 162
Cac8I GCNNGC 1 cut(s) 65
Csp6I GTAC 1 cut(s) 43
CviAII CATG 1 cut(s) 242
CviJI RGCY 3 cut(s) 58, 100, 105
CviKI_1 RGCY 3 cut(s) 58, 100, 105
CviQI GTAC 1 cut(s) 43
DdeI CTNAG 2 cut(s) 59, 173
DpnI GATC 2 cut(s) 30, 238
DpnII GATC 2 cut(s) 28, 236
EaeI YGGCCR 1 cut(s) 103
EagI CGGCCG 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 103
Eco52I CGGCCG 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 232
FaeI CATG 1 cut(s) 245
FaiI YATR 6 cut(s) 23, 149, 169, 243, 284, 297
FatI CATG 1 cut(s) 241
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 1 cut(s) 56
FokI GGATG 1 cut(s) 24
Fsp4HI GCNGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 56
GsuI CTGGAG 1 cut(s) 216
HaeIII GGCC 1 cut(s) 105
HapII CCGG 2 cut(s) 32, 106
Hin1II CATG 1 cut(s) 245
HincII GTYRAC 2 cut(s) 91, 217
HindII GTYRAC 2 cut(s) 91, 217
HinfI GANTC 4 cut(s) 77, 109, 254, 266
HpaII CCGG 2 cut(s) 32, 106
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 3 cut(s) 91, 123, 217
Hpy188I TCNGA 2 cut(s) 73, 114
Hpy188III TCNNGA 2 cut(s) 13, 32
Hpy8I GTNNAC 3 cut(s) 91, 123, 217
Hpy99I CGWCG 2 cut(s) 92, 95
HpyCH4III ACNGT 2 cut(s) 88, 157
HpyCH4IV ACGT 1 cut(s) 119
HpyCH4V TGCA 2 cut(s) 144, 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 64
HpyF3I CTNAG 2 cut(s) 59, 173
HpySE526I ACGT 1 cut(s) 119
Hsp92II CATG 1 cut(s) 245
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 2 cut(s) 28, 236
LmnI GCTCC 1 cut(s) 34
LpnPI CCDG 7 cut(s) 45, 119, 185, 219, 243, 246, 276
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 2 cut(s) 46, 296
MaeII ACGT 1 cut(s) 119
MalI GATC 2 cut(s) 30, 238
MboI GATC 2 cut(s) 28, 236
MboII GAAGA 1 cut(s) 263
MflI RGATCY 2 cut(s) 28, 236
MluCI AATT 1 cut(s) 7
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 4 cut(s) 9, 67, 210, 213
MroI TCCGGA 1 cut(s) 31
MseI TTAA 3 cut(s) 180, 222, 301
MslI CAYNNNNRTG 1 cut(s) 246
MspI CCGG 2 cut(s) 32, 106
MspR9I CCNGG 1 cut(s) 234
MvaI CCWGG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 64
NdeII GATC 2 cut(s) 28, 236
NlaIII CATG 1 cut(s) 245
NmeAIII GCCGAG 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 125
PfeI GAWTC 3 cut(s) 109, 254, 266
PfoI TCCNGGA 1 cut(s) 232
PkrI GCNGC 1 cut(s) 57
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 232
PspGI CCWGG 1 cut(s) 232
PsuI RGATCY 2 cut(s) 28, 236
RsaI GTAC 1 cut(s) 44
RsaNI GTAC 1 cut(s) 43
RseI CAYNNNNRTG 1 cut(s) 246
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 3 cut(s) 180, 222, 301
SatI GCNGC 1 cut(s) 56
Sau3AI GATC 2 cut(s) 28, 236
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 1 cut(s) 234
SetI ASST 7 cut(s) 60, 102, 122, 216, 228, 262, 295
SfaNI GCATC 2 cut(s) 46, 296
SgrDI CGTCGACG 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 246
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
Sse9I AATT 1 cut(s) 7
StyD4I CCNGG 1 cut(s) 232
TaaI ACNGT 2 cut(s) 88, 157
TaiI ACGT 1 cut(s) 122
TaqI TCGA 1 cut(s) 90
TasI AATT 1 cut(s) 7
TfiI GAWTC 3 cut(s) 109, 254, 266
Tru1I TTAA 3 cut(s) 180, 222, 301
Tru9I TTAA 3 cut(s) 180, 222, 301
TscAI CASTG 1 cut(s) 162
TseI GCWGC 1 cut(s) 55
TspDTI ATGAA 1 cut(s) 264
TspGWI ACGGA 2 cut(s) 30, 261
TspRI CASTG 1 cut(s) 162
XapI RAATTY 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.