pycom02g13660

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005216
Physical Location & Seq
Forward (+)
10248734 .. 10251421
2688 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1302 bp
ATGGAGAAAAGAAGCTCAACCCCAAGATATGGAAACCTCGTAACGATTCTCAGTATAGACGGGGGAGGAATTAGAGGGATCATCCCTGGTGTTCTTCTCACTTACCTTGAATCTGAACTTCAGAAACTAGATGGTGAAGATGCAAGGCTTGCAGATTATTTTGACGTCATTTCTGGGACAAGTACAGGCGGTCTAATTGCGACCATGTTAGTAGCTCCAAACGAAAACAACAGACCATTATTCGCTGCAAAAGACATTGTCCCATTTTACCATTCCCACTGCCCTAAAATTTTCCCACAATCAAGTGGACTTTGTACCACAGTTAAAGATCTAGTGAAAGCTCTAACAGGACCAAAGTATGATGGAAAGTATTTGCACAAGCTTGTAAGAGAGATAATAGGAGACAAAAGGTTGGACCAAACCTTGACGAACCTGGTTGTCCCAACTTTTGACATCAAGATACTTCTGCCTGTCATATTTTCGTCTTATCAGCTAAGTGCTATTCTCATGATCAAGGTGTCAAGTCGTCCAGTGCTGAATGCCAGACTTTCAGACATTTGCATTGGTACCTCAGCAGCACCAACTTACCTTCCTGCATACTATTTTGAGAACAAGGATCAACAAGGAGAATCCAAAGAGTTCAACCTCATCGATGGTGGCATAGCCGCGAATAATCCGGTGATGCTCTTGCACAATAATTTGCAGAATGTAAAGTTTTCATTGATTGCCATTAGTGAAGTGATAAAACAAATTACGAGCCAGAATCAAGATTACTTGGAGATTGAGCCAAAGGACTTGTATGATCGTTTCCTACTGATTTCACTGGGAACGGGTTCGGACAGAAGTGAACTCAAATACAATGCCAAGAAAGCTTCAAAATGGAATGTTATAAGCTGGTTGTACACTAATGGGTCAACTCCTTTGTTGGATTGCTTTGATGAAGCAACTTGCGATATGGTTGATTACCATAACTCTGCGGTTTTCCAAGCTCTCCGTTCCGAACAGAACTACCTCAGAATTGACGTAAGTTTGAAATATCTGATTAAAAACGAAAAACGAAAAACGAAATGGTATCAAACGAAGGGAGATGTAGCTTCAGTTGATAAAGCTACACGGGAAAACTTGGAAAATCTAGTGGAAGTGGGAAACAAATTACTCAAGAAACCAGTTTGTCGCGTGAATTTGGATACTTGTCTCTATGAACCAGTTGAAGATGAGGGCGCAAATGAAGAAGCACTTCAGAGGTTTGCAAAGTTACTGTCGGATGAAAAGAAGCTTCGGGAGTCAAAATCATCACAGTAA

Protein Analysis

434

Amino Acids

48.55

Weight (kDa)

6.57

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 17 - 230 1e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
AatII GACGTC 1 cut(s) 168
Acc65I GGTACC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 566
AccB7I CCANNNNNTGG 1 cut(s) 29
AccII CGCG 2 cut(s) 668, 1176
AciI CCGC 3 cut(s) 189, 666, 977
AclWI GGATC 2 cut(s) 86, 624
AcsI RAATTY 2 cut(s) 288, 1180
AcuI CTGAAG 3 cut(s) 104, 1080, 1223
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 4 cut(s) 184, 316, 568, 902
AfiI CCNNNNNNNGG 1 cut(s) 29
AgsI TTSAA 5 cut(s) 110, 643, 876, 1033, 1211
AjnI CCWGG 2 cut(s) 85, 432
Alw26I GTCTC 2 cut(s) 396, 1199
AlwI GGATC 2 cut(s) 86, 624
ApeKI GCWGC 2 cut(s) 245, 575
ApoI RAATTY 2 cut(s) 288, 1180
ArsI GACNNNNNNTTYG 2 cut(s) 1244, 1276
Asp700I GAANNNNTTC 2 cut(s) 832, 1236
Asp718I GGTACC 1 cut(s) 566
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 2 cut(s) 350, 415
AsuHPI GGTGA 2 cut(s) 146, 691
AvaII GGWCC 2 cut(s) 350, 415
BanI GGYRCC 1 cut(s) 566
BbvCI CCTCAGC 1 cut(s) 571
BbvI GCAGC 2 cut(s) 232, 587
BccI CCATC 3 cut(s) 125, 356, 647
BciT130I CCWGG 2 cut(s) 87, 434
BciVI GTATCC 1 cut(s) 1180
BclI TGATCA 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 396, 1199
BfaI CTAG 3 cut(s) 128, 332, 1133
BfuI GTATCC 1 cut(s) 1180
BglII AGATCT 1 cut(s) 328
BisI GCNGC 3 cut(s) 246, 576, 666
BlsI GCNGC 3 cut(s) 247, 577, 667
Bme1390I CCNGG 2 cut(s) 87, 434
Bme18I GGWCC 2 cut(s) 350, 415
BmgT120I GGNCC 2 cut(s) 350, 415
BmiI GGNNCC 1 cut(s) 568
BmrFI CCNGG 2 cut(s) 87, 434
BmrI ACTGGG 1 cut(s) 833
BmsI GCATC 2 cut(s) 130, 672
BmuI ACTGGG 1 cut(s) 833
Bpu10I CCTNAGC 1 cut(s) 571
BpuEI CTTGAG 1 cut(s) 1142
Bsa29I ATCGAT 1 cut(s) 651
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 676
Bsc4I CCNNNNNNNGG 1 cut(s) 29
Bse1I ACTGG 4 cut(s) 530, 828, 1166, 1205
BseBI CCWGG 2 cut(s) 87, 434
BseCI ATCGAT 1 cut(s) 651
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 2 cut(s) 81, 1270
BseLI CCNNNNNNNGG 1 cut(s) 29
BseMII CTCAG 3 cut(s) 64, 585, 1027
BseNI ACTGG 4 cut(s) 530, 828, 1166, 1205
BseXI GCAGC 2 cut(s) 232, 587
Bsh1236I CGCG 2 cut(s) 668, 1176
BshNI GGYRCC 1 cut(s) 566
BshVI ATCGAT 1 cut(s) 651
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 3 cut(s) 190, 245, 425
BslI CCNNNNNNNGG 1 cut(s) 29
BsmAI GTCTC 2 cut(s) 396, 1199
BsmFI GGGAC 3 cut(s) 190, 245, 425
BsmI GAATGC 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 900
Bsp143I GATC 5 cut(s) 78, 328, 510, 616, 802
BspACI CCGC 3 cut(s) 189, 666, 977
BspCNI CTCAG 3 cut(s) 63, 584, 1026
BspDI ATCGAT 1 cut(s) 651
BspFNI CGCG 2 cut(s) 668, 1176
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 1 cut(s) 568
BspPI GGATC 2 cut(s) 86, 624
BspT107I GGYRCC 1 cut(s) 566
BsrGI TGTACA 1 cut(s) 900
BsrI ACTGG 4 cut(s) 530, 828, 1166, 1205
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 5 cut(s) 78, 328, 510, 616, 802
BssNI GRCGYC 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 87, 434
Bst4CI ACNGT 3 cut(s) 322, 1260, 1299
BstACI GRCGYC 1 cut(s) 165
BstAPI GCANNNNNTGC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 900
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 4 cut(s) 50, 494, 571, 1013
BstF5I GGATG 2 cut(s) 81, 1270
BstFNI CGCG 2 cut(s) 668, 1176
BstHHI GCGC 1 cut(s) 1223
BstKTI GATC 5 cut(s) 81, 331, 513, 619, 805
BstMAI GTCTC 2 cut(s) 396, 1199
BstMBI GATC 5 cut(s) 78, 328, 510, 616, 802
BstMWI GCNNNNNNNGC 2 cut(s) 149, 869
BstNI CCWGG 2 cut(s) 87, 434
BstSCI CCNGG 2 cut(s) 85, 432
BstUI CGCG 2 cut(s) 668, 1176
BstV1I GCAGC 2 cut(s) 232, 587
BstX2I RGATCY 1 cut(s) 328
BstYI RGATCY 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 651
BsuI GTATCC 1 cut(s) 1180
BsuTUI ATCGAT 1 cut(s) 651
BtsCI GGATG 2 cut(s) 81, 1270
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 3 cut(s) 277, 537, 821
Cac8I GCNNGC 1 cut(s) 150
CciI TCATGA 1 cut(s) 507
CfoI GCGC 1 cut(s) 1223
Cfr13I GGNCC 2 cut(s) 350, 415
ClaI ATCGAT 1 cut(s) 651
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 4 cut(s) 183, 315, 567, 901
CviAII CATG 2 cut(s) 205, 508
CviQI GTAC 4 cut(s) 183, 315, 567, 901
DdeI CTNAG 4 cut(s) 50, 494, 571, 1013
DpnI GATC 5 cut(s) 80, 330, 512, 618, 804
DpnII GATC 5 cut(s) 78, 328, 510, 616, 802
Eco47I GGWCC 2 cut(s) 350, 415
Eco57I CTGAAG 3 cut(s) 104, 1080, 1223
EcoRII CCWGG 2 cut(s) 85, 432
FaeI CATG 2 cut(s) 208, 511
FalI AAGNNNNNCTT 2 cut(s) 1221, 1253
FaqI GGGAC 3 cut(s) 190, 245, 425
FatI CATG 2 cut(s) 204, 507
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 246, 576, 666
FokI GGATG 2 cut(s) 68, 1277
Fsp4HI GCNGC 3 cut(s) 246, 576, 666
FspBI CTAG 3 cut(s) 128, 332, 1133
GlaI GCGC 1 cut(s) 1222
GluI GCNGC 3 cut(s) 246, 576, 666
HapII CCGG 1 cut(s) 677
HhaI GCGC 1 cut(s) 1223
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 2 cut(s) 208, 511
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HindIII AAGCTT 3 cut(s) 380, 870, 1274
HinfI GANTC 5 cut(s) 46, 110, 629, 763, 1283
HpaII CCGG 1 cut(s) 677
HphI GGTGA 2 cut(s) 146, 691
Hpy166II GTNNAC 4 cut(s) 308, 848, 903, 915
Hpy188I TCNGA 9 cut(s) 115, 123, 553, 838, 1000, 1016, 1041, 1242, 1264
Hpy188III TCNNGA 5 cut(s) 457, 508, 767, 1159, 1280
Hpy8I GTNNAC 4 cut(s) 308, 848, 903, 915
HpyAV CCTTC 2 cut(s) 599, 1075
HpyCH4III ACNGT 3 cut(s) 322, 1260, 1299
HpyCH4IV ACGT 2 cut(s) 165, 1023
HpyCH4V TGCA 9 cut(s) 143, 152, 248, 376, 561, 596, 691, 703, 1250
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 869
HpyF3I CTNAG 4 cut(s) 50, 494, 571, 1013
HpySE526I ACGT 2 cut(s) 165, 1023
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 2 cut(s) 208, 511
HspAI GCGC 1 cut(s) 1221
KpnI GGTACC 1 cut(s) 570
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 5 cut(s) 78, 328, 510, 616, 802
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 2 cut(s) 232, 587
LweI GCATC 2 cut(s) 130, 672
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 3 cut(s) 128, 332, 1133
MaeII ACGT 2 cut(s) 165, 1023
MaeIII GTNAC 2 cut(s) 40, 1254
MalI GATC 5 cut(s) 80, 330, 512, 618, 804
MboI GATC 5 cut(s) 78, 328, 510, 616, 802
MboII GAAGA 4 cut(s) 86, 149, 1223, 1241
MflI RGATCY 1 cut(s) 328
MluCI AATT 8 cut(s) 69, 195, 288, 697, 750, 1017, 1151, 1180
MlyI GAGTC 1 cut(s) 1292
MmeI TCCRAC 3 cut(s) 393, 906, 1242
MnlI CCTC 8 cut(s) 47, 59, 68, 580, 656, 1022, 1210, 1236
MroXI GAANNNNTTC 2 cut(s) 832, 1236
MseI TTAA 2 cut(s) 324, 1044
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 2 cut(s) 87, 434
Mva1269I GAATGC 1 cut(s) 544
MvaI CCWGG 2 cut(s) 87, 434
MvnI CGCG 2 cut(s) 668, 1176
MwoI GCNNNNNNNGC 2 cut(s) 149, 869
NdeII GATC 5 cut(s) 78, 328, 510, 616, 802
NlaIII CATG 2 cut(s) 208, 511
NlaIV GGNNCC 1 cut(s) 568
PagI TCATGA 1 cut(s) 507
PctI GAATGC 1 cut(s) 544
PdmI GAANNNNTTC 2 cut(s) 832, 1236
PfeI GAWTC 4 cut(s) 46, 110, 629, 763
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 29
PkrI GCNGC 3 cut(s) 247, 577, 667
PleI GAGTC 1 cut(s) 1291
PpsI GAGTC 1 cut(s) 1291
PsiI TTATAA 1 cut(s) 890
Psp6I CCWGG 2 cut(s) 85, 432
PspGI CCWGG 2 cut(s) 85, 432
PspN4I GGNNCC 1 cut(s) 568
PspPI GGNCC 2 cut(s) 350, 415
PsuI RGATCY 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 4 cut(s) 184, 316, 568, 902
RsaNI GTAC 4 cut(s) 183, 315, 567, 901
SaqAI TTAA 2 cut(s) 324, 1044
SatI GCNGC 3 cut(s) 246, 576, 666
Sau3AI GATC 5 cut(s) 78, 328, 510, 616, 802
Sau96I GGNCC 2 cut(s) 350, 415
SchI GAGTC 1 cut(s) 1292
ScrFI CCNGG 2 cut(s) 87, 434
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 2 cut(s) 130, 672
SinI GGWCC 2 cut(s) 350, 415
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
Sse9I AATT 8 cut(s) 69, 195, 288, 697, 750, 1017, 1151, 1180
SsiI CCGC 3 cut(s) 189, 666, 977
SspMI CTAG 3 cut(s) 128, 332, 1133
StyD4I CCNGG 2 cut(s) 85, 432
TaaI ACNGT 3 cut(s) 322, 1260, 1299
TaiI ACGT 2 cut(s) 168, 1026
TaqI TCGA 1 cut(s) 651
TasI AATT 8 cut(s) 69, 195, 288, 697, 750, 1017, 1151, 1180
TatI WGTACW 2 cut(s) 182, 900
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 4 cut(s) 46, 110, 629, 763
Tru1I TTAA 2 cut(s) 324, 1044
Tru9I TTAA 2 cut(s) 324, 1044
TscAI CASTG 3 cut(s) 284, 537, 828
TseI GCWGC 2 cut(s) 245, 575
TspDTI ATGAA 5 cut(s) 708, 954, 1215, 1242, 1281
TspGWI ACGGA 1 cut(s) 983
TspRI CASTG 3 cut(s) 284, 537, 828
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 350, 415
XapI RAATTY 2 cut(s) 288, 1180
XmnI GAANNNNTTC 2 cut(s) 832, 1236
XspI CTAG 3 cut(s) 128, 332, 1133
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.