pycom02g14310

Polysaccharide biosynthesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014161
Physical Location & Seq
Forward (+)
10903417 .. 10904106
690 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14310.1

Sequence Viewer

Length: 690 bp
ATGTCGTACGCCGACATCAAGCCCATCTCTGATGCGCTCCGAAAATGCTCCACCCCATGCAACTTGCTCATTTTCGGGCTTACCCACGAAACCTTACTCTGGAAGGCCCTAAACAACAACGGCCGTACCGTGTACATCGACGAGAACCGGTACTACGCCGCCTACATGGAGGAGAAGCACCCCGAAATCGACGCCTACGACGTGCAGTACACGACAAAGGTGAAGGAGACCAAGGAGCTCATCGCCGTGGCAAAAGAGCAAATACGCAACGAGTGCCGGCCGGTGCAGAATCTGCTCTTCTCCGAGTGCAAGCTGGGGATCAACGACCTGCCCAACCACGTGTACGACGTCGAGTGGGACGTGATCCTGGTGGACGGGCCCCGTGGCGGGTGGCCGGACGCGCCCGGGAGAGTGATGCCGATATTCACGTCGGGGGTGCTGGCCAGGAGCAAGAAGGGTGGGAACGGGAAGACGCACGTGTTTGTGCATGATATTGGCGGGACGGAGGTGAGGGTTGCTGGGAACGAGTTTTTGTGTCGGGAGAACTTGGTGGAGGCGACGGAGACGATGGGGCATTACGTGTTGGAGAGAATGGAGGAGAGTAGCTTTCAGTTCTGCGGCAACCGCACGACGTCGTCTTCGTCAGCAGCAAAGAAATCTTCTGCTTCTTCTTCTTCAGCATCGTCTTAA

Protein Analysis

230

Amino Acids

25.49

Weight (kDa)

5.9

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IRX15_IRX15L_GXM PF21729 5 - 193 6.9e-75 IRX15/IRX15L/GXM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014161)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50220 AT5G67210
fragaria_vesca FvH4_1g16440
malus_domestica MD02G1177100.v1.1 MD15G1287000.v1.1
prunus_persica Prupe.6G230900_v2.0.a1
pyrus_communis pycom02g14310 pycom15g25040
rosa_chinensis RchiOBHm_Chr2g0106321
rosa_laevigata RLG00000017488
rosa_roxburghii Rroxscaffold_2G00137280
rosa_rugosa Rorug02G0134600
rosa_samantha Rh2AG185700 Rh2BG196000 Rh2CG190800 Rh2DG191800
rosa_wichuraiana Rw2G014480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 351, 635
Acc36I ACCTGC 1 cut(s) 336
AccII CGCG 1 cut(s) 401
AciI CCGC 5 cut(s) 159, 387, 498, 618, 625
AclWI GGATC 2 cut(s) 326, 358
AcoI YGGCCR 4 cut(s) 121, 278, 392, 441
AcuI CTGAAG 1 cut(s) 660
AcvI CACGTG 2 cut(s) 340, 478
AcyI GRCGYC 3 cut(s) 192, 348, 632
AfaI GTAC 6 cut(s) 8, 127, 134, 152, 209, 344
AfiI CCNNNNNNNGG 3 cut(s) 99, 386, 387
AflIII ACRYGT 3 cut(s) 339, 477, 579
AgeI ACCGGT 1 cut(s) 147
AjiI CACGTC 3 cut(s) 202, 361, 429
AjnI CCWGG 2 cut(s) 366, 443
AluBI AGCT 3 cut(s) 238, 313, 606
AluI AGCT 3 cut(s) 238, 313, 606
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 2 cut(s) 221, 557
AlwI GGATC 2 cut(s) 326, 358
AlwNI CAGNNNCTG 1 cut(s) 292
Ama87I CYCGRG 1 cut(s) 404
AoxI GGCC 6 cut(s) 105, 121, 278, 377, 392, 441
ApaI GGGCCC 1 cut(s) 381
ApeKI GCWGC 1 cut(s) 647
ArsI GACNNNNNNTTYG 2 cut(s) 622, 654
AsiGI ACCGGT 1 cut(s) 147
AspLEI GCGC 2 cut(s) 37, 403
AspS9I GGNCC 3 cut(s) 106, 377, 378
AsuC2I CCSGG 2 cut(s) 405, 406
AsuHPI GGTGA 2 cut(s) 232, 520
AvaI CYCGRG 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 381
BaeI ACNNNNGTAYC 2 cut(s) 109, 142
BalI TGGCCA 1 cut(s) 443
BanII GRGCYC 2 cut(s) 240, 381
BbrPI CACGTG 2 cut(s) 340, 478
BbsI GAAGAC 2 cut(s) 476, 630
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 659
BccI CCATC 2 cut(s) 32, 562
BceAI ACGGC 3 cut(s) 108, 136, 230
BciT130I CCWGG 2 cut(s) 368, 445
BcnI CCSGG 2 cut(s) 405, 406
BcoDI GTCTC 2 cut(s) 221, 557
BfuAI ACCTGC 1 cut(s) 336
BisI GCNGC 3 cut(s) 159, 619, 648
BlsI GCNGC 3 cut(s) 160, 620, 649
Bme1390I CCNGG 4 cut(s) 368, 405, 406, 445
BmeT110I CYCGRG 1 cut(s) 404
BmgBI CACGTC 3 cut(s) 202, 361, 429
BmgT120I GGNCC 3 cut(s) 106, 377, 378
BmiI GGNNCC 2 cut(s) 379, 380
BmrFI CCNGG 4 cut(s) 368, 405, 406, 445
BmsI GCATC 2 cut(s) 22, 405
BpiI GAAGAC 2 cut(s) 476, 630
BpuMI CCSGG 2 cut(s) 405, 406
BsaAI YACGTR 3 cut(s) 340, 478, 580
BsaHI GRCGYC 3 cut(s) 192, 348, 632
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 4 cut(s) 231, 246, 382, 404
BsaWI WCCGGW 1 cut(s) 147
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 386, 387
Bse118I RCCGGY 3 cut(s) 147, 276, 280
BseBI CCWGG 2 cut(s) 368, 445
BseDI CCNNGG 4 cut(s) 231, 246, 382, 404
BseLI CCNNNNNNNGG 3 cut(s) 99, 386, 387
BseRI GAGGAG 2 cut(s) 185, 611
BseSI GKGCMC 1 cut(s) 381
BseX3I CGGCCG 2 cut(s) 121, 278
BseXI GCAGC 1 cut(s) 659
BseYI CCCAGC 2 cut(s) 313, 518
BsgI GTGCAG 2 cut(s) 224, 305
Bsh1236I CGCG 1 cut(s) 401
Bsh1285I CGRYCG 2 cut(s) 124, 281
BshFI GGCC 6 cut(s) 107, 123, 280, 379, 394, 443
BshTI ACCGGT 1 cut(s) 147
BsiEI CGRYCG 2 cut(s) 124, 281
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 5 cut(s) 148, 277, 281, 395, 405
BsiWI CGTACG 1 cut(s) 6
BslFI GGGAC 2 cut(s) 371, 514
BslI CCNNNNNNNGG 3 cut(s) 99, 386, 387
BsmAI GTCTC 2 cut(s) 221, 557
BsmBI CGTCTC 1 cut(s) 557
BsmFI GGGAC 2 cut(s) 371, 514
BsnI GGCC 6 cut(s) 107, 123, 280, 379, 394, 443
Bso31I GGTCTC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 404
Bsp120I GGGCCC 1 cut(s) 377
Bsp1286I GDGCHC 2 cut(s) 240, 381
Bsp1407I TGTACA 1 cut(s) 132
Bsp143I GATC 2 cut(s) 318, 363
BspACI CCGC 5 cut(s) 159, 387, 498, 618, 625
BspANI GGCC 6 cut(s) 107, 123, 280, 379, 394, 443
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 2 cut(s) 379, 380
BspMI ACCTGC 1 cut(s) 336
BspPI GGATC 2 cut(s) 326, 358
BspQI GCTCTTC 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 221
BsrFI RCCGGY 3 cut(s) 147, 276, 280
BsrGI TGTACA 1 cut(s) 132
BssAI RCCGGY 3 cut(s) 147, 276, 280
BssECI CCNNGG 4 cut(s) 231, 246, 382, 404
BssMI GATC 2 cut(s) 318, 363
BssNI GRCGYC 3 cut(s) 192, 348, 632
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 2 cut(s) 368, 445
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 1 cut(s) 302
BstACI GRCGYC 3 cut(s) 192, 348, 632
BstAPI GCANNNNNTGC 2 cut(s) 273, 292
BstAUI TGTACA 1 cut(s) 132
BstBAI YACGTR 3 cut(s) 340, 478, 580
BstC8I GCNNGC 3 cut(s) 278, 311, 441
BstDSI CCRYGG 2 cut(s) 246, 382
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 2 cut(s) 37, 403
BstKTI GATC 2 cut(s) 321, 366
BstMAI GTCTC 2 cut(s) 221, 557
BstMBI GATC 2 cut(s) 318, 363
BstMCI CGRYCG 2 cut(s) 124, 281
BstMWI GCNNNNNNNGC 4 cut(s) 273, 292, 400, 624
BstNI CCWGG 2 cut(s) 368, 445
BstSCI CCNGG 4 cut(s) 366, 403, 404, 443
BstSLI GKGCMC 1 cut(s) 381
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 2 cut(s) 476, 630
BstZI CGGCCG 2 cut(s) 121, 278
BsuRI GGCC 6 cut(s) 107, 123, 280, 379, 394, 443
BtgI CCRYGG 2 cut(s) 246, 382
BtgZI GCGATG 1 cut(s) 226
BtrI CACGTC 3 cut(s) 202, 361, 429
BveI ACCTGC 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 278, 311, 441
CaiI CAGNNNCTG 1 cut(s) 292
CfoI GCGC 2 cut(s) 37, 403
Cfr10I RCCGGY 3 cut(s) 147, 276, 280
Cfr13I GGNCC 3 cut(s) 106, 377, 378
Cfr9I CCCGGG 1 cut(s) 404
CseI GACGC 3 cut(s) 200, 407, 481
Csp6I GTAC 6 cut(s) 7, 126, 133, 151, 208, 343
CspAI ACCGGT 1 cut(s) 147
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviAII CATG 3 cut(s) 57, 166, 488
CviQI GTAC 6 cut(s) 7, 126, 133, 151, 208, 343
DpnI GATC 2 cut(s) 320, 365
DpnII GATC 2 cut(s) 318, 363
EaeI YGGCCR 4 cut(s) 121, 278, 392, 441
EagI CGGCCG 2 cut(s) 121, 278
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Ecl136II GAGCTC 1 cut(s) 238
EclXI CGGCCG 2 cut(s) 121, 278
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 2 cut(s) 240, 381
Eco31I GGTCTC 1 cut(s) 221
Eco52I CGGCCG 2 cut(s) 121, 278
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 660
Eco72I CACGTG 2 cut(s) 340, 478
Eco88I CYCGRG 1 cut(s) 404
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 2 cut(s) 106, 378
EcoRII CCWGG 2 cut(s) 366, 443
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 2 cut(s) 240, 381
ErhI CCWWGG 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 3 cut(s) 60, 169, 491
FaiI YATR 3 cut(s) 58, 167, 489
FaqI GGGAC 2 cut(s) 371, 514
FatI CATG 3 cut(s) 56, 165, 487
FauI CCCGC 2 cut(s) 380, 491
Fnu4HI GCNGC 3 cut(s) 159, 619, 648
FriOI GRGCYC 2 cut(s) 240, 381
Fsp4HI GCNGC 3 cut(s) 159, 619, 648
GlaI GCGC 2 cut(s) 36, 402
GluI GCNGC 3 cut(s) 159, 619, 648
GsaI CCCAGC 2 cut(s) 317, 522
HaeIII GGCC 6 cut(s) 107, 123, 280, 379, 394, 443
HapII CCGG 5 cut(s) 148, 277, 281, 395, 405
HgaI GACGC 3 cut(s) 200, 407, 481
HhaI GCGC 2 cut(s) 37, 403
Hin1I GRCGYC 3 cut(s) 192, 348, 632
Hin1II CATG 3 cut(s) 60, 169, 491
Hin6I GCGC 2 cut(s) 35, 401
HinP1I GCGC 2 cut(s) 35, 401
HinfI GANTC 1 cut(s) 289
HpaII CCGG 5 cut(s) 148, 277, 281, 395, 405
HphI GGTGA 2 cut(s) 232, 520
Hpy166II GTNNAC 4 cut(s) 133, 210, 343, 373
Hpy188I TCNGA 3 cut(s) 31, 41, 304
Hpy188III TCNNGA 2 cut(s) 100, 539
Hpy8I GTNNAC 4 cut(s) 133, 210, 343, 373
Hpy99I CGWCG 9 cut(s) 143, 194, 203, 350, 353, 433, 562, 634, 637
HpyAV CCTTC 3 cut(s) 97, 217, 448
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 8 cut(s) 201, 339, 348, 360, 428, 477, 579, 632
HpyCH4V TGCA 5 cut(s) 60, 205, 286, 309, 487
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 292, 400, 624
HpySE526I ACGT 8 cut(s) 201, 339, 348, 360, 428, 477, 579, 632
Hsp92I GRCGYC 3 cut(s) 192, 348, 632
Hsp92II CATG 3 cut(s) 60, 169, 491
HspAI GCGC 2 cut(s) 35, 401
KroI GCCGGC 1 cut(s) 276
KroNI GCCGGC 1 cut(s) 278
Kzo9I GATC 2 cut(s) 318, 363
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 4 cut(s) 42, 53, 235, 447
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 2 cut(s) 22, 405
MaeII ACGT 8 cut(s) 201, 339, 348, 360, 428, 477, 579, 632
MalI GATC 2 cut(s) 320, 365
MboI GATC 2 cut(s) 318, 363
MboII GAAGA 7 cut(s) 289, 481, 630, 651, 660, 663, 666
MhlI GDGCHC 2 cut(s) 240, 381
MlsI TGGCCA 1 cut(s) 443
MluNI TGGCCA 1 cut(s) 443
MmeI TCCRAC 1 cut(s) 564
MnlI CCTC 5 cut(s) 163, 499, 504, 547, 589
Mox20I TGGCCA 1 cut(s) 443
MroNI GCCGGC 1 cut(s) 276
MscI TGGCCA 1 cut(s) 443
MseI TTAA 1 cut(s) 688
MslI CAYNNNNRTG 1 cut(s) 245
Msp20I TGGCCA 1 cut(s) 443
MspI CCGG 5 cut(s) 148, 277, 281, 395, 405
MspR9I CCNGG 4 cut(s) 368, 405, 406, 445
MvaI CCWGG 2 cut(s) 368, 445
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 4 cut(s) 273, 292, 400, 624
NaeI GCCGGC 1 cut(s) 278
NciI CCSGG 2 cut(s) 405, 406
NdeII GATC 2 cut(s) 318, 363
NgoMIV GCCGGC 1 cut(s) 276
NlaIII CATG 3 cut(s) 60, 169, 491
NlaIV GGNNCC 2 cut(s) 379, 380
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 6 cut(s) 126, 195, 198, 345, 357, 638
PdiI GCCGGC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 289
Pfl23II CGTACG 1 cut(s) 6
PflFI GACNNNGTC 1 cut(s) 634
PinAI ACCGGT 1 cut(s) 147
PkrI GCNGC 3 cut(s) 160, 620, 649
PmaCI CACGTG 2 cut(s) 340, 478
PmlI CACGTG 2 cut(s) 340, 478
Ppu21I YACGTR 3 cut(s) 340, 478, 580
Psp124BI GAGCTC 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 366, 443
PspCI CACGTG 2 cut(s) 340, 478
PspFI CCCAGC 2 cut(s) 313, 518
PspGI CCWGG 2 cut(s) 366, 443
PspLI CGTACG 1 cut(s) 6
PspN4I GGNNCC 2 cut(s) 379, 380
PspOMI GGGCCC 1 cut(s) 377
PspPI GGNCC 3 cut(s) 106, 377, 378
PsrI GAACNNNNNNTAC 2 cut(s) 137, 169
PstNI CAGNNNCTG 1 cut(s) 292
PsyI GACNNNGTC 1 cut(s) 634
RsaI GTAC 6 cut(s) 8, 127, 134, 152, 209, 344
RsaNI GTAC 6 cut(s) 7, 126, 133, 151, 208, 343
RseI CAYNNNNRTG 1 cut(s) 245
SacI GAGCTC 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 688
SatI GCNGC 3 cut(s) 159, 619, 648
Sau3AI GATC 2 cut(s) 318, 363
Sau96I GGNCC 3 cut(s) 106, 377, 378
ScrFI CCNGG 4 cut(s) 368, 405, 406, 445
SduI GDGCHC 2 cut(s) 240, 381
SfaNI GCATC 2 cut(s) 22, 405
SmaI CCCGGG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 245
SsiI CCGC 5 cut(s) 159, 387, 498, 618, 625
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 4 cut(s) 366, 403, 404, 443
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 8 cut(s) 204, 342, 351, 363, 431, 480, 582, 635
TaqI TCGA 3 cut(s) 138, 189, 351
TatI WGTACW 2 cut(s) 132, 207
TauI GCSGC 2 cut(s) 161, 621
TfiI GAWTC 1 cut(s) 289
Tru1I TTAA 1 cut(s) 688
Tru9I TTAA 1 cut(s) 688
TseI GCWGC 1 cut(s) 647
TspGWI ACGGA 2 cut(s) 518, 575
TspMI CCCGGG 1 cut(s) 404
Tth111I GACNNNGTC 1 cut(s) 634
XmaI CCCGGG 1 cut(s) 404
ZraI GACGTC 2 cut(s) 349, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.