pycom02g15670

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004363
Physical Location & Seq
Forward (+)
12590304 .. 12594948
4645 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g15670.3

Sequence Viewer

Length: 879 bp
ATGGAATGTGCTGAGGAGAGAAGCAGAGAGCTAGCCCAAGCGTCTACCTTTTACCGTGCAGTGTACTCAGAGATAGAGGAAGTTGGATGGGAGAATTTAGCGAGGTTTAGTGGAGATCTAACATCTCTCAGCTTTCGGATAATAGATGGCAAGGGCAGAGTGCATGTTGTGGAAATTCAGTTGGATAAAACACATCCCAAATGTCCTCCTTCAATTTCAGCAGATGTGCCATCTTTGTTTAATTTAAAATGGTCATCAAACTCAAGATTGAAAGATGTGGTGCGGCAGTTTAAAAAGCATCTGGAGAACCTTCAGGCTTTTTGGTCTACTTTGGATGATATCGACAGGTTGCTTTGGGTTGTTGATCGATCTAAACAAGCATCTCCTGCTGTGTCATATCGCCAAATTATTATAGGAAATGATTGCTGCATCACGTTGTTCATTAATTCCGTTGATCCTAGATCATTACCAGAATGTCGTTTTATTGGTTCAGGTCCATTTGTGAATACGTTGAGAAAGAGATGGCGAAGAAATAGCAAAAGATGGATAAAAGACAAACCATTTCTGGAAAATCTTGAATATCTTCTGGAGACTCAACTGCCAATGCCCCCTGATGTCGAGAAGAATGACCAGCAAGTTGAATGTGGAATTTGTTATGCTCAGAGTCTTCCAATTGATGACGAGCTCAGAGATAAGAATGGAACCGGGACTGACTGTACATGTGATAATACCAGCTGCAACAAGGCTTTCCACAGCATATGTCTTGTGGACTGGTTGCGTTCTATCACCACAACACGGCAGTCATTTGATGTGTTATTTGGAAGCTGTCCATACTGTTCAGAACCAGTTGCGGTGAAAATCAACAGCGTCAAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04120 map03460 map04120
Pfam Domains
Protein Families
FANCL_C WD-3

Protein Analysis

293

Amino Acids

33.48

Weight (kDa)

6.88

Isoelectric Point (pI)

59.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 44, 326
AciI CCGC 2 cut(s) 283, 851
AclWI GGATC 1 cut(s) 449
AcsI RAATTY 3 cut(s) 94, 174, 648
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 2 cut(s) 65, 718
AfiI CCNNNNNNNGG 1 cut(s) 795
AflIII ACRYGT 1 cut(s) 719
AgsI TTSAA 4 cut(s) 213, 271, 578, 641
AluBI AGCT 5 cut(s) 31, 132, 685, 735, 825
AluI AGCT 5 cut(s) 31, 132, 685, 735, 825
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 1 cut(s) 584
AlwI GGATC 1 cut(s) 449
ApeKI GCWGC 2 cut(s) 426, 735
ApoI RAATTY 3 cut(s) 94, 174, 648
AseI ATTAAT 1 cut(s) 444
Asp700I GAANNNNTTC 1 cut(s) 582
AspS9I GGNCC 1 cut(s) 494
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 2 cut(s) 778, 865
AsuNHI GCTAGC 1 cut(s) 31
AvaII GGWCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 687
BbsI GAAGAC 1 cut(s) 659
Bbv12I GWGCWC 1 cut(s) 687
BbvCI CCTCAGC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 413, 722
BccI CCATC 5 cut(s) 81, 140, 238, 516, 537
BceAI ACGGC 1 cut(s) 812
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 584
BfaI CTAG 2 cut(s) 32, 459
BglII AGATCT 1 cut(s) 115
BisI GCNGC 3 cut(s) 284, 427, 736
BlsI GCNGC 3 cut(s) 285, 428, 737
Bme1390I CCNGG 1 cut(s) 706
Bme18I GGWCC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 1 cut(s) 706
BmsI GCATC 3 cut(s) 307, 389, 438
BmtI GCTAGC 1 cut(s) 35
BpiI GAAGAC 1 cut(s) 659
BpmI CTGGAG 2 cut(s) 323, 608
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 247
BpuMI CCSGG 1 cut(s) 706
Bsa29I ATCGAT 1 cut(s) 367
Bsc4I CCNNNNNNNGG 1 cut(s) 795
Bse1I ACTGG 2 cut(s) 776, 845
BseCI ATCGAT 1 cut(s) 367
BseGI GGATG 3 cut(s) 92, 193, 340
BseLI CCNNNNNNNGG 1 cut(s) 795
BseMII CTCAG 4 cut(s) 81, 142, 674, 700
BseNI ACTGG 2 cut(s) 776, 845
BseRI GAGGAG 1 cut(s) 29
BseXI GCAGC 2 cut(s) 413, 722
BsgI GTGCAG 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 367
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 1 cut(s) 705
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 1 cut(s) 795
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 721
Bsp1286I GDGCHC 1 cut(s) 687
Bsp1407I TGTACA 1 cut(s) 716
Bsp143I GATC 5 cut(s) 115, 364, 368, 454, 461
BspACI CCGC 2 cut(s) 283, 851
BspCNI CTCAG 5 cut(s) 4, 80, 141, 673, 699
BspDI ATCGAT 1 cut(s) 367
BspLI GGNNCC 1 cut(s) 703
BspOI GCTAGC 1 cut(s) 35
BspPI GGATC 1 cut(s) 449
BsrGI TGTACA 1 cut(s) 716
BsrI ACTGG 2 cut(s) 776, 845
BssMI GATC 5 cut(s) 115, 364, 368, 454, 461
Bst4CI ACNGT 3 cut(s) 56, 716, 836
BstAPI GCANNNNNTGC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 716
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 5 cut(s) 12, 67, 128, 660, 686
BstF5I GGATG 3 cut(s) 92, 193, 340
BstKTI GATC 5 cut(s) 118, 367, 371, 457, 464
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 5 cut(s) 115, 364, 368, 454, 461
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNSI RCATGY 2 cut(s) 167, 723
BstSCI CCNGG 1 cut(s) 704
BstV1I GCAGC 2 cut(s) 413, 722
BstV2I GAAGAC 1 cut(s) 659
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 367
BsuTUI ATCGAT 1 cut(s) 367
BtsCI GGATG 3 cut(s) 92, 193, 340
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 494
ClaI ATCGAT 1 cut(s) 367
CseI GACGC 2 cut(s) 30, 856
Csp6I GTAC 2 cut(s) 64, 717
CviAII CATG 2 cut(s) 164, 720
CviJI RGCY 8 cut(s) 31, 35, 132, 317, 685, 735, 746, 825
CviKI_1 RGCY 8 cut(s) 31, 35, 132, 317, 685, 735, 746, 825
CviQI GTAC 2 cut(s) 64, 717
DdeI CTNAG 5 cut(s) 12, 67, 128, 660, 686
DpnI GATC 5 cut(s) 117, 366, 370, 456, 463
DpnII GATC 5 cut(s) 115, 364, 368, 454, 461
DraI TTTAAA 2 cut(s) 246, 292
Ecl136II GAGCTC 1 cut(s) 685
Eco24I GRGCYC 1 cut(s) 687
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 494
Eco53kI GAGCTC 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 685
EcoRV GATATC 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 687
FaeI CATG 2 cut(s) 167, 723
FaiI YATR 8 cut(s) 165, 397, 413, 657, 721, 758, 760, 832
FaqI GGGAC 1 cut(s) 721
FatI CATG 2 cut(s) 163, 719
FauNDI CATATG 1 cut(s) 758
FblI GTMKAC 2 cut(s) 44, 326
Fnu4HI GCNGC 3 cut(s) 284, 427, 736
FokI GGATG 3 cut(s) 99, 180, 347
FriOI GRGCYC 1 cut(s) 687
Fsp4HI GCNGC 3 cut(s) 284, 427, 736
FspBI CTAG 2 cut(s) 32, 459
GluI GCNGC 3 cut(s) 284, 427, 736
GsuI CTGGAG 2 cut(s) 323, 608
HapII CCGG 1 cut(s) 705
HgaI GACGC 2 cut(s) 30, 856
Hin1II CATG 2 cut(s) 167, 723
HinfI GANTC 2 cut(s) 592, 664
HpaII CCGG 1 cut(s) 705
HphI GGTGA 2 cut(s) 778, 865
Hpy166II GTNNAC 4 cut(s) 45, 64, 327, 769
Hpy188I TCNGA 5 cut(s) 70, 138, 663, 689, 841
Hpy188III TCNNGA 7 cut(s) 264, 302, 566, 575, 587, 619, 871
Hpy8I GTNNAC 4 cut(s) 45, 64, 327, 769
HpyAV CCTTC 2 cut(s) 219, 320
HpyCH4III ACNGT 3 cut(s) 56, 716, 836
HpyCH4IV ACGT 2 cut(s) 434, 509
HpyCH4V TGCA 4 cut(s) 59, 163, 429, 738
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 5 cut(s) 12, 67, 128, 660, 686
HpySE526I ACGT 2 cut(s) 434, 509
Hsp92II CATG 2 cut(s) 167, 723
Kzo9I GATC 5 cut(s) 115, 364, 368, 454, 461
Lsp1109I GCAGC 2 cut(s) 413, 722
LweI GCATC 3 cut(s) 307, 389, 438
MaeI CTAG 2 cut(s) 32, 459
MaeII ACGT 2 cut(s) 434, 509
MalI GATC 5 cut(s) 117, 366, 370, 456, 463
MboI GATC 5 cut(s) 115, 364, 368, 454, 461
MboII GAAGA 4 cut(s) 540, 575, 634, 659
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 8 cut(s) 94, 174, 213, 241, 405, 445, 648, 672
MlyI GAGTC 2 cut(s) 586, 673
MmeI TCCRAC 2 cut(s) 64, 162
MnlI CCTC 5 cut(s) 7, 70, 96, 216, 867
MroXI GAANNNNTTC 1 cut(s) 582
MseI TTAA 4 cut(s) 240, 245, 291, 444
MspA1I CMGCKG 1 cut(s) 735
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 1 cut(s) 706
MunI CAATTG 1 cut(s) 672
MwoI GCNNNNNNNGC 1 cut(s) 386
NciI CCSGG 1 cut(s) 706
NdeI CATATG 1 cut(s) 758
NdeII GATC 5 cut(s) 115, 364, 368, 454, 461
NheI GCTAGC 1 cut(s) 31
NlaIII CATG 2 cut(s) 167, 723
NlaIV GGNNCC 1 cut(s) 703
NspI RCATGY 2 cut(s) 167, 723
PciI ACATGT 1 cut(s) 719
PdmI GAANNNNTTC 1 cut(s) 582
PkrI GCNGC 3 cut(s) 285, 428, 737
PleI GAGTC 2 cut(s) 586, 672
PpsI GAGTC 2 cut(s) 586, 672
PscI ACATGT 1 cut(s) 719
PshBI ATTAAT 1 cut(s) 444
Psp124BI GAGCTC 1 cut(s) 687
PspN4I GGNNCC 1 cut(s) 703
PspPI GGNCC 1 cut(s) 494
PsuI RGATCY 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 735
RsaI GTAC 2 cut(s) 65, 718
RsaNI GTAC 2 cut(s) 64, 717
SacI GAGCTC 1 cut(s) 687
SaqAI TTAA 4 cut(s) 240, 245, 291, 444
SatI GCNGC 3 cut(s) 284, 427, 736
Sau3AI GATC 5 cut(s) 115, 364, 368, 454, 461
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 2 cut(s) 586, 673
ScrFI CCNGG 1 cut(s) 706
SduI GDGCHC 1 cut(s) 687
SfaNI GCATC 3 cut(s) 307, 389, 438
SinI GGWCC 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
Sse9I AATT 8 cut(s) 94, 174, 213, 241, 405, 445, 648, 672
SsiI CCGC 2 cut(s) 283, 851
SspMI CTAG 2 cut(s) 32, 459
SstI GAGCTC 1 cut(s) 687
StyD4I CCNGG 1 cut(s) 704
TaaI ACNGT 3 cut(s) 56, 716, 836
TaiI ACGT 2 cut(s) 437, 512
TaqI TCGA 3 cut(s) 342, 367, 618
TasI AATT 8 cut(s) 94, 174, 213, 241, 405, 445, 648, 672
TatI WGTACW 2 cut(s) 63, 716
TauI GCSGC 1 cut(s) 286
Tru1I TTAA 4 cut(s) 240, 245, 291, 444
Tru9I TTAA 4 cut(s) 240, 245, 291, 444
TscAI CASTG 1 cut(s) 66
TseI GCWGC 2 cut(s) 426, 735
TspDTI ATGAA 1 cut(s) 430
TspGWI ACGGA 1 cut(s) 439
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 494
VspI ATTAAT 1 cut(s) 444
XapI RAATTY 3 cut(s) 94, 174, 648
XceI RCATGY 2 cut(s) 167, 723
XmiI GTMKAC 2 cut(s) 44, 326
XmnI GAANNNNTTC 1 cut(s) 582
XspI CTAG 2 cut(s) 32, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.