RLG00000017727

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004363
Physical Location & Seq
Forward (+)
20523583 .. 20527517
3935 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017727

Sequence Viewer

Length: 990 bp
ATGAATTGTGAGCAAGGTCATTCGTATGAATTATATGAACTGTGTAAAGACTCAACCTTTACTGCTCTACGACGACTCCTCCCGAGGTCGAACCAAATGACTGCTGAAGCGCAGGAATGTAGTGAAGAGAGTAGATGCAGAGAGCTGGCCAAAGCCTCTACCTTTTACCGTGCAGTCTACTCTGAGATAGAGGAAGTTGGGTGGGAGAATCTCGTGAGATTTGGTGGAGATCTAACATCTCTCAGCTTTCAGGTCTTAGATAGTAAGGGCAGAGTGCATGTTATGGAAATTCAGTTGGATAAAACCCATCCTACCTATCCTCCTTCAATTTCAGCAGATGTACCTTATTTGTTTAATTTAAAATGGTCATCAAACTCAAGATTGAAAGATGTGGTTCAGCAGTTTAGAAAGCATCTGGAGAAGCTTCAGACATTTTGGTCTACTTTGGATGAGATTGATAGGTCCCTATGTGTTGTTGAACCTAAACAAGCATCCCCTGCTGTATCCAATCACCAAGTCATTATAGGAAATGATTGTTTCGTCATGTTGTCTATTAATGCCACTGATCCTAGATCTTTACCAGAGTGTCGTTTTATTGGTACAGGTCAAATCGTGAACCTTTTGAGAGACAGATGGCAGAGAAATAGCAAAAGATGGGTAAAAGACAAAGAATTTCTGGTTAATCTTGAAACTCTTCTGGAGACTCAGCTGCCAAAGCCTGCAGATGTTGAGAAGCATGATCAGCAAGTGGAATGCGGAATTTGTTATGCTCCGAGTCTTCCAGTTGATGATGAGCTCAGAGGTAAGAGTGGAACTGGGACTGACTATAAATGCGATAATACCAGCTGCAAGAGGGCTTTTCATAGCATATGTCTCGTGGACTGGCTGCGTTCCATTACCACAACAAGGCGGTCATTTGATGTTTTATTTGGAAGTTGTCCATACTGTTCAGAACCAGTTGCAGTGAAAATCAGTAGTGTGAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 ko04120 map03460 map04120
Pfam Domains
Protein Families
FANCL_C WD-3

Protein Analysis

330

Amino Acids

37.61

Weight (kDa)

6.41

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FANCL_d2 PF18890 54 - 142 1.5e-19 FANCL UBC-like domain 2
FANCL_d3 PF18891 145 - 240 2.4e-21 FANCL UBC-like domain 3
FANCL_C PF11793 248 - 325 6.5e-29 FANCL C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 436
AccI GTMKAC 2 cut(s) 177, 440
AciI CCGC 2 cut(s) 756, 910
AclWI GGATC 1 cut(s) 560
AcoI YGGCCR 1 cut(s) 147
AcsI RAATTY 3 cut(s) 288, 671, 759
AcuI CTGAAG 2 cut(s) 126, 410
AfaI GTAC 2 cut(s) 342, 601
AfiI CCNNNNNNNGG 1 cut(s) 906
AgsI TTSAA 4 cut(s) 327, 385, 479, 689
AluBI AGCT 6 cut(s) 145, 246, 424, 709, 796, 846
AluI AGCT 6 cut(s) 145, 246, 424, 709, 796, 846
Alw21I GWGCWC 1 cut(s) 798
Alw26I GTCTC 3 cut(s) 621, 695, 878
AlwI GGATC 1 cut(s) 560
Ama87I CYCGRG 1 cut(s) 82
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 3 cut(s) 709, 846, 886
ApoI RAATTY 3 cut(s) 288, 671, 759
AseI ATTAAT 1 cut(s) 555
Asp700I GAANNNNTTC 1 cut(s) 693
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 503
AvaI CYCGRG 1 cut(s) 82
AvaII GGWCC 1 cut(s) 462
BalI TGGCCA 1 cut(s) 149
BanII GRGCYC 1 cut(s) 798
BauI CACGAG 2 cut(s) 212, 875
BbsI GAAGAC 1 cut(s) 770
Bbv12I GWGCWC 1 cut(s) 798
BbvI GCAGC 3 cut(s) 696, 833, 873
BccI CCATC 3 cut(s) 315, 627, 648
BcgI CGANNNNNNTGC 2 cut(s) 856, 890
BciVI GTATCC 1 cut(s) 514
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 3 cut(s) 621, 695, 878
BfaI CTAG 1 cut(s) 570
BfmI CTRYAG 1 cut(s) 720
BfuI GTATCC 1 cut(s) 514
BglII AGATCT 2 cut(s) 229, 572
BisI GCNGC 3 cut(s) 710, 847, 887
BlsI GCNGC 3 cut(s) 711, 848, 888
Bme18I GGWCC 1 cut(s) 462
BmeT110I CYCGRG 1 cut(s) 82
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 464
BmrI ACTGGG 1 cut(s) 825
BmsI GCATC 3 cut(s) 125, 421, 500
BmuI ACTGGG 1 cut(s) 825
BpiI GAAGAC 1 cut(s) 770
BpmI CTGGAG 2 cut(s) 437, 719
BpuEI CTTGAG 1 cut(s) 361
BsaJI CCNNGG 1 cut(s) 83
BsaXI ACNNNNNCTCC 6 cut(s) 60, 63, 90, 93, 304, 334
Bsc4I CCNNNNNNNGG 1 cut(s) 906
Bse1I ACTGG 4 cut(s) 782, 820, 887, 956
BseDI CCNNGG 1 cut(s) 83
BseGI GGATG 3 cut(s) 307, 454, 491
BseLI CCNNNNNNNGG 1 cut(s) 906
BseMII CTCAG 4 cut(s) 174, 256, 719, 811
BseNI ACTGG 4 cut(s) 782, 820, 887, 956
BseRI GAGGAG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 696, 833, 873
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 798
BsiHKCI CYCGRG 1 cut(s) 82
BslFI GGGAC 2 cut(s) 448, 832
BslI CCNNNNNNNGG 1 cut(s) 906
BsmAI GTCTC 3 cut(s) 621, 695, 878
BsmFI GGGAC 2 cut(s) 448, 832
BsmI GAATGC 1 cut(s) 758
BsnI GGCC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 82
Bsp1286I GDGCHC 1 cut(s) 798
Bsp143I GATC 4 cut(s) 229, 565, 572, 739
BspACI CCGC 2 cut(s) 756, 910
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 4 cut(s) 175, 255, 718, 810
BspLI GGNNCC 1 cut(s) 464
BspMAI CTGCAG 1 cut(s) 724
BspPI GGATC 1 cut(s) 560
BsrI ACTGG 4 cut(s) 782, 820, 887, 956
BssECI CCNNGG 1 cut(s) 83
BssMI GATC 4 cut(s) 229, 565, 572, 739
BssSI CACGAG 2 cut(s) 212, 875
Bst2BI CACGAG 2 cut(s) 212, 875
Bst4CI ACNGT 3 cut(s) 42, 170, 947
Bst6I CTCTTC 2 cut(s) 120, 699
BstAPI GCANNNNNTGC 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 147, 720
BstDEI CTNAG 5 cut(s) 183, 242, 256, 705, 797
BstF5I GGATG 3 cut(s) 307, 454, 491
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 4 cut(s) 232, 568, 575, 742
BstMAI GTCTC 3 cut(s) 621, 695, 878
BstMBI GATC 4 cut(s) 229, 565, 572, 739
BstMWI GCNNNNNNNGC 3 cut(s) 497, 715, 742
BstNSI RCATGY 1 cut(s) 281
BstSFI CTRYAG 1 cut(s) 720
BstV1I GCAGC 3 cut(s) 696, 833, 873
BstV2I GAAGAC 1 cut(s) 770
BstX2I RGATCY 2 cut(s) 229, 572
BstYI RGATCY 2 cut(s) 229, 572
BsuI GTATCC 1 cut(s) 514
BsuRI GGCC 1 cut(s) 149
BtsCI GGATG 3 cut(s) 307, 454, 491
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 2 cut(s) 561, 969
Cac8I GCNNGC 2 cut(s) 147, 720
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 462
Csp6I GTAC 2 cut(s) 341, 600
CviAII CATG 3 cut(s) 278, 544, 737
CviQI GTAC 2 cut(s) 341, 600
DdeI CTNAG 5 cut(s) 183, 242, 256, 705, 797
DpnI GATC 4 cut(s) 231, 567, 574, 741
DpnII GATC 4 cut(s) 229, 565, 572, 739
DraI TTTAAA 1 cut(s) 360
DrdI GACNNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 120, 699
EarI CTCTTC 2 cut(s) 120, 699
Ecl136II GAGCTC 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 462
Eco53kI GAGCTC 1 cut(s) 796
Eco57I CTGAAG 2 cut(s) 126, 410
Eco88I CYCGRG 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 796
EcoO109I RGGNCCY 1 cut(s) 462
EcoT38I GRGCYC 1 cut(s) 798
FaeI CATG 3 cut(s) 281, 547, 740
FaqI GGGAC 2 cut(s) 448, 832
FatI CATG 3 cut(s) 277, 543, 736
FauNDI CATATG 1 cut(s) 869
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 2 cut(s) 177, 440
Fnu4HI GCNGC 3 cut(s) 710, 847, 887
FokI GGATG 3 cut(s) 294, 461, 478
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 3 cut(s) 710, 847, 887
FspBI CTAG 1 cut(s) 570
GlaI GCGC 1 cut(s) 111
GluI GCNGC 3 cut(s) 710, 847, 887
GsuI CTGGAG 2 cut(s) 437, 719
HaeIII GGCC 1 cut(s) 149
HhaI GCGC 1 cut(s) 112
Hin1II CATG 3 cut(s) 281, 547, 740
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 5 cut(s) 50, 75, 208, 703, 775
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 4 cut(s) 178, 441, 616, 880
Hpy188I TCNGA 5 cut(s) 184, 429, 774, 800, 952
Hpy188III TCNNGA 7 cut(s) 82, 214, 378, 416, 613, 686, 698
Hpy8I GTNNAC 4 cut(s) 178, 441, 616, 880
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 1 cut(s) 333
HpyCH4III ACNGT 3 cut(s) 42, 170, 947
HpyCH4V TGCA 6 cut(s) 138, 173, 277, 722, 849, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 715, 742
HpyF3I CTNAG 5 cut(s) 183, 242, 256, 705, 797
Hsp92II CATG 3 cut(s) 281, 547, 740
HspAI GCGC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 4 cut(s) 229, 565, 572, 739
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 3 cut(s) 696, 833, 873
LweI GCATC 3 cut(s) 125, 421, 500
MaeI CTAG 1 cut(s) 570
MalI GATC 4 cut(s) 231, 567, 574, 741
MboI GATC 4 cut(s) 229, 565, 572, 739
MboII GAAGA 3 cut(s) 137, 686, 770
MflI RGATCY 2 cut(s) 229, 572
MhlI GDGCHC 1 cut(s) 798
MlsI TGGCCA 1 cut(s) 149
MluCI AATT 7 cut(s) 4, 29, 288, 327, 355, 671, 759
MluNI TGGCCA 1 cut(s) 149
MlyI GAGTC 4 cut(s) 44, 69, 697, 784
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 7 cut(s) 78, 89, 166, 184, 330, 794, 846
Mox20I TGGCCA 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 693
MscI TGGCCA 1 cut(s) 149
MseI TTAA 4 cut(s) 354, 359, 555, 681
MslI CAYNNNNRTG 1 cut(s) 24
Msp20I TGGCCA 1 cut(s) 149
MspA1I CMGCKG 2 cut(s) 709, 846
Mva1269I GAATGC 1 cut(s) 758
MwoI GCNNNNNNNGC 3 cut(s) 497, 715, 742
NdeI CATATG 1 cut(s) 869
NdeII GATC 4 cut(s) 229, 565, 572, 739
NlaIII CATG 3 cut(s) 281, 547, 740
NlaIV GGNNCC 1 cut(s) 464
NspI RCATGY 1 cut(s) 281
PctI GAATGC 1 cut(s) 758
PdmI GAANNNNTTC 1 cut(s) 693
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 3 cut(s) 711, 848, 888
PleI GAGTC 4 cut(s) 44, 69, 697, 783
PpsI GAGTC 4 cut(s) 44, 69, 697, 783
PpuMI RGGWCCY 1 cut(s) 462
PshBI ATTAAT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 798
Psp5II RGGWCCY 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 464
PspPI GGNCC 1 cut(s) 462
PspPPI RGGWCCY 1 cut(s) 462
PstI CTGCAG 1 cut(s) 724
PsuI RGATCY 2 cut(s) 229, 572
PvuII CAGCTG 2 cut(s) 709, 846
RsaI GTAC 2 cut(s) 342, 601
RsaNI GTAC 2 cut(s) 341, 600
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 798
SaqAI TTAA 4 cut(s) 354, 359, 555, 681
SatI GCNGC 3 cut(s) 710, 847, 887
Sau3AI GATC 4 cut(s) 229, 565, 572, 739
Sau96I GGNCC 1 cut(s) 462
SchI GAGTC 4 cut(s) 44, 69, 697, 784
SduI GDGCHC 1 cut(s) 798
SfaNI GCATC 3 cut(s) 125, 421, 500
SfcI CTRYAG 1 cut(s) 720
SinI GGWCC 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 7 cut(s) 4, 29, 288, 327, 355, 671, 759
SsiI CCGC 2 cut(s) 756, 910
SspMI CTAG 1 cut(s) 570
SstI GAGCTC 1 cut(s) 798
TaaI ACNGT 3 cut(s) 42, 170, 947
TaqI TCGA 1 cut(s) 89
TasI AATT 7 cut(s) 4, 29, 288, 327, 355, 671, 759
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 4 cut(s) 354, 359, 555, 681
Tru9I TTAA 4 cut(s) 354, 359, 555, 681
TscAI CASTG 2 cut(s) 568, 969
TseI GCWGC 3 cut(s) 709, 846, 886
TspDTI ATGAA 4 cut(s) 17, 42, 51, 851
TspRI CASTG 2 cut(s) 568, 969
VpaK11BI GGWCC 1 cut(s) 462
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 3 cut(s) 288, 671, 759
XceI RCATGY 1 cut(s) 281
XmiI GTMKAC 2 cut(s) 177, 440
XmnI GAANNNNTTC 1 cut(s) 693
XspI CTAG 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.