pycom02g15700

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014640
Physical Location & Seq
Reverse (-)
12652476 .. 12654594
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
ATGTGTAAATCGTGTAAAAAGAGATACAAACACATTGCGCACAAAGACAGAGAGACAGAGGGCCAGTTTCGATTCCTACCTTTTCTTTCTTTATTAATTTCGAAAAAATATCAGCAAAAAAAAAAAAAAAAAAAAAAAAGCATCCAAAATCTGTGCCTCTTTTGCTTTACTATATGTTCTGCGACCTACCGAGCTTCTGGTCCGCAAGGACGCAAAGCTCCGCGATTTGCATCTGAGATGGCGTCGTCGGCGCAGCTCCGGCTCATGTCCGATCTCAGAACCATCATCAACGAGCCCCCCGAGGGTTGCAGCGCCAGCCCTGTATCTGATGACAATCTGATGGTGTGGAGCGCCACCATATTCGGGCCTGATGAGACCCCATGGGAAGGAGGTGTTTTTAGCTTGAGGTTGACATTTAGTGAACGTTATCCCGAAAAGCCGCCTCGGGTTCGTTTCACCTCCGAAATTTTCCATCCAAATGTTTATCGTGATGGTTCACTGTGCATGGATATAATTCAGGATGCATGGTCACCATGCCACAATGTATCTACGATTTTGACGTCTGTTCAATCACTTCTCACTGATCCAAACGCTGCAAGTCCAGCAAACCCTGATGCTGCTCACATGTATCAGCATGATATACAAGCTTACAACAAGAGGGTACGCGGTTGTGCTCGTCATTCCATTGAGTCTTTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

234

Amino Acids

26.45

Weight (kDa)

9.3

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 87 - 223 2.2e-49 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 563
Acc16I TGCGCA 1 cut(s) 39
AccII CGCG 2 cut(s) 223, 666
AciI CCGC 4 cut(s) 203, 221, 440, 666
AclI AACGTT 1 cut(s) 424
AclWI GGATC 1 cut(s) 578
AcsI RAATTY 1 cut(s) 465
AcyI GRCGYC 2 cut(s) 242, 560
AfaI GTAC 1 cut(s) 663
AfiI CCNNNNNNNGG 3 cut(s) 302, 363, 386
AflIII ACRYGT 1 cut(s) 624
AgsI TTSAA 1 cut(s) 569
AluBI AGCT 5 cut(s) 194, 218, 256, 402, 647
AluI AGCT 5 cut(s) 194, 218, 256, 402, 647
Alw21I GWGCWC 1 cut(s) 676
Alw26I GTCTC 2 cut(s) 47, 368
AlwI GGATC 1 cut(s) 578
Ama87I CYCGRG 2 cut(s) 299, 444
AoxI GGCC 2 cut(s) 61, 365
ApeKI GCWGC 4 cut(s) 253, 309, 593, 617
ApoI RAATTY 1 cut(s) 465
AseI ATTAAT 1 cut(s) 95
AspLEI GCGC 4 cut(s) 40, 253, 314, 353
AspS9I GGNCC 3 cut(s) 61, 200, 365
AsuHPI GGTGA 2 cut(s) 448, 522
AsuII TTCGAA 1 cut(s) 101
AvaI CYCGRG 2 cut(s) 299, 444
AvaII GGWCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 297
Bbv12I GWGCWC 1 cut(s) 676
BbvI GCAGC 4 cut(s) 265, 321, 580, 604
BccI CCATC 5 cut(s) 232, 290, 334, 480, 485
BcoDI GTCTC 2 cut(s) 47, 368
BfoI RGCGCY 2 cut(s) 315, 354
BisI GCNGC 5 cut(s) 254, 310, 440, 594, 618
BlsI GCNGC 5 cut(s) 255, 311, 441, 595, 619
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 2 cut(s) 299, 444
BmgT120I GGNCC 3 cut(s) 61, 200, 365
BmsI GCATC 4 cut(s) 150, 239, 511, 604
Bpu14I TTCGAA 1 cut(s) 101
BpuEI CTTGAG 1 cut(s) 424
BsaBI GATNNNNATC 2 cut(s) 229, 333
BsaHI GRCGYC 2 cut(s) 242, 560
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 300, 380, 443
Bsc4I CCNNNNNNNGG 3 cut(s) 302, 363, 386
Bse1I ACTGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 33
Bse8I GATNNNNATC 2 cut(s) 229, 333
BseDI CCNNGG 3 cut(s) 300, 380, 443
BseGI GGATG 3 cut(s) 141, 472, 526
BseJI GATNNNNATC 2 cut(s) 229, 333
BseLI CCNNNNNNNGG 3 cut(s) 302, 363, 386
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 2 cut(s) 225, 289
BseNI ACTGG 1 cut(s) 64
BseXI GCAGC 4 cut(s) 265, 321, 580, 604
Bsh1236I CGCG 2 cut(s) 223, 666
BshFI GGCC 2 cut(s) 63, 367
BsiHKAI GWGCWC 1 cut(s) 676
BsiHKCI CYCGRG 2 cut(s) 299, 444
BsiSI CCGG 1 cut(s) 259
BslI CCNNNNNNNGG 3 cut(s) 302, 363, 386
BsmAI GTCTC 2 cut(s) 47, 368
BsnI GGCC 2 cut(s) 63, 367
Bso31I GGTCTC 1 cut(s) 368
BsoBI CYCGRG 2 cut(s) 299, 444
Bsp119I TTCGAA 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 297, 676
Bsp143I GATC 2 cut(s) 271, 583
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 4 cut(s) 203, 221, 440, 666
BspANI GGCC 2 cut(s) 63, 367
BspCNI CTCAG 2 cut(s) 226, 288
BspFNI CGCG 2 cut(s) 223, 666
BspPI GGATC 1 cut(s) 578
BspT104I TTCGAA 1 cut(s) 101
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 33
BsrI ACTGG 1 cut(s) 64
BssECI CCNNGG 3 cut(s) 300, 380, 443
BssMI GATC 2 cut(s) 271, 583
BssNI GRCGYC 2 cut(s) 242, 560
BssT1I CCWWGG 1 cut(s) 380
Bst4CI ACNGT 1 cut(s) 501
BstACI GRCGYC 2 cut(s) 242, 560
BstBI TTCGAA 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 2 cut(s) 234, 275
BstDSI CCRYGG 1 cut(s) 380
BstEII GGTNACC 1 cut(s) 528
BstF5I GGATG 3 cut(s) 141, 472, 526
BstFNI CGCG 2 cut(s) 223, 666
BstH2I RGCGCY 2 cut(s) 315, 354
BstHHI GCGC 4 cut(s) 40, 253, 314, 353
BstKTI GATC 2 cut(s) 274, 586
BstMAI GTCTC 2 cut(s) 47, 368
BstMBI GATC 2 cut(s) 271, 583
BstMWI GCNNNNNNNGC 5 cut(s) 162, 248, 259, 315, 602
BstNSI RCATGY 1 cut(s) 628
BstPI GGTNACC 1 cut(s) 528
BstUI CGCG 2 cut(s) 223, 666
BstV1I GCAGC 4 cut(s) 265, 321, 580, 604
BsuRI GGCC 2 cut(s) 63, 367
BtgI CCRYGG 1 cut(s) 380
BtsCI GGATG 3 cut(s) 141, 472, 526
BtsIMutI CAGTG 2 cut(s) 497, 579
Cac8I GCNNGC 1 cut(s) 316
CfoI GCGC 4 cut(s) 40, 253, 314, 353
Cfr13I GGNCC 3 cut(s) 61, 200, 365
CseI GACGC 2 cut(s) 219, 231
Csp6I GTAC 1 cut(s) 662
CviAII CATG 7 cut(s) 265, 381, 505, 525, 534, 625, 635
CviQI GTAC 1 cut(s) 662
DdeI CTNAG 2 cut(s) 234, 275
DpnI GATC 2 cut(s) 273, 585
DpnII GATC 2 cut(s) 271, 583
Eco130I CCWWGG 1 cut(s) 380
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 200
Eco88I CYCGRG 2 cut(s) 299, 444
Eco91I GGTNACC 1 cut(s) 528
EcoO65I GGTNACC 1 cut(s) 528
EcoT14I CCWWGG 1 cut(s) 380
EcoT22I ATGCAT 1 cut(s) 526
EcoT38I GRGCYC 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 7 cut(s) 268, 384, 508, 528, 537, 628, 638
FatI CATG 7 cut(s) 264, 380, 504, 524, 533, 624, 634
Fnu4HI GCNGC 5 cut(s) 254, 310, 440, 594, 618
FokI GGATG 3 cut(s) 128, 459, 533
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 5 cut(s) 254, 310, 440, 594, 618
FspI TGCGCA 1 cut(s) 39
GlaI GCGC 4 cut(s) 39, 252, 313, 352
GluI GCNGC 5 cut(s) 254, 310, 440, 594, 618
HaeII RGCGCY 2 cut(s) 315, 354
HaeIII GGCC 2 cut(s) 63, 367
HapII CCGG 1 cut(s) 259
HgaI GACGC 2 cut(s) 219, 231
HhaI GCGC 4 cut(s) 40, 253, 314, 353
Hin1I GRCGYC 2 cut(s) 242, 560
Hin1II CATG 7 cut(s) 268, 384, 508, 528, 537, 628, 638
Hin6I GCGC 4 cut(s) 38, 251, 312, 351
HinP1I GCGC 4 cut(s) 38, 251, 312, 351
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 2 cut(s) 72, 689
HpaII CCGG 1 cut(s) 259
HphI GGTGA 2 cut(s) 448, 522
Hpy166II GTNNAC 3 cut(s) 411, 422, 497
Hpy188I TCNGA 6 cut(s) 235, 271, 278, 328, 339, 463
Hpy188III TCNNGA 3 cut(s) 431, 488, 518
Hpy8I GTNNAC 3 cut(s) 411, 422, 497
Hpy99I CGWCG 2 cut(s) 247, 250
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 1 cut(s) 501
HpyCH4IV ACGT 2 cut(s) 424, 560
HpyCH4V TGCA 6 cut(s) 230, 309, 504, 524, 596, 697
HpyF10VI GCNNNNNNNGC 5 cut(s) 162, 248, 259, 315, 602
HpyF3I CTNAG 2 cut(s) 234, 275
HpySE526I ACGT 2 cut(s) 424, 560
Hsp92I GRCGYC 2 cut(s) 242, 560
Hsp92II CATG 7 cut(s) 268, 384, 508, 528, 537, 628, 638
HspAI GCGC 4 cut(s) 38, 251, 312, 351
Kzo9I GATC 2 cut(s) 271, 583
LmnI GCTCC 3 cut(s) 223, 261, 348
LpnPI CCDG 9 cut(s) 77, 183, 272, 328, 333, 381, 503, 615, 624
Lsp1109I GCAGC 4 cut(s) 265, 321, 580, 604
LweI GCATC 4 cut(s) 150, 239, 511, 604
MaeII ACGT 2 cut(s) 424, 560
MaeIII GTNAC 1 cut(s) 528
MalI GATC 2 cut(s) 273, 585
MboI GATC 2 cut(s) 271, 583
MhlI GDGCHC 2 cut(s) 297, 676
MluCI AATT 3 cut(s) 96, 465, 513
MlyI GAGTC 1 cut(s) 698
MnlI CCTC 8 cut(s) 52, 167, 295, 383, 399, 453, 469, 651
Mph1103I ATGCAT 1 cut(s) 526
MseI TTAA 1 cut(s) 95
MslI CAYNNNNRTG 1 cut(s) 477
MspI CCGG 1 cut(s) 259
MvnI CGCG 2 cut(s) 223, 666
MwoI GCNNNNNNNGC 5 cut(s) 162, 248, 259, 315, 602
NcoI CCATGG 1 cut(s) 380
NdeII GATC 2 cut(s) 271, 583
NlaIII CATG 7 cut(s) 268, 384, 508, 528, 537, 628, 638
NmuCI GTSAC 1 cut(s) 528
NsbI TGCGCA 1 cut(s) 39
NsiI ATGCAT 1 cut(s) 526
NspI RCATGY 1 cut(s) 628
NspV TTCGAA 1 cut(s) 101
PciI ACATGT 1 cut(s) 624
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 557
PfeI GAWTC 1 cut(s) 72
PkrI GCNGC 5 cut(s) 255, 311, 441, 595, 619
PleI GAGTC 1 cut(s) 697
PpsI GAGTC 1 cut(s) 697
PscI ACATGT 1 cut(s) 624
PshBI ATTAAT 1 cut(s) 95
Psp1406I AACGTT 1 cut(s) 424
PspEI GGTNACC 1 cut(s) 528
PspPI GGNCC 3 cut(s) 61, 200, 365
RsaI GTAC 1 cut(s) 663
RsaNI GTAC 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 477
SaqAI TTAA 1 cut(s) 95
SatI GCNGC 5 cut(s) 254, 310, 440, 594, 618
Sau3AI GATC 2 cut(s) 271, 583
Sau96I GGNCC 3 cut(s) 61, 200, 365
SchI GAGTC 1 cut(s) 698
SduI GDGCHC 2 cut(s) 297, 676
SfaNI GCATC 4 cut(s) 150, 239, 511, 604
SfuI TTCGAA 1 cut(s) 101
SinI GGWCC 1 cut(s) 200
SmiMI CAYNNNNRTG 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 3 cut(s) 96, 465, 513
SsiI CCGC 4 cut(s) 203, 221, 440, 666
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 1 cut(s) 501
TaiI ACGT 2 cut(s) 427, 563
TaqI TCGA 2 cut(s) 70, 101
TasI AATT 3 cut(s) 96, 465, 513
TauI GCSGC 1 cut(s) 442
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 1 cut(s) 95
Tru9I TTAA 1 cut(s) 95
TscAI CASTG 2 cut(s) 504, 586
TseFI GTSAC 1 cut(s) 528
TseI GCWGC 4 cut(s) 253, 309, 593, 617
Tsp45I GTSAC 1 cut(s) 528
TspRI CASTG 2 cut(s) 504, 586
VpaK11BI GGWCC 1 cut(s) 200
VspI ATTAAT 1 cut(s) 95
XapI RAATTY 1 cut(s) 465
XceI RCATGY 1 cut(s) 628
ZraI GACGTC 1 cut(s) 561
Zsp2I ATGCAT 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.