pycom02g19400

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006723
Physical Location & Seq
Forward (+)
17228556 .. 17230739
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1104 bp
ATGGCCATGCCAGATAGCCAAGAATCGGTCTTACCCAAAGAAGAAACTGTGATTGAACAAACACTATTTGTTAAACTTGGTCGTTGGCAATGGTGGTTTCTGGTGGTACTCAACGTCTTCTTCCTAATTGTGGGGCAGACTGCTGCTGTTTTACTTGGAAGATTCTATTATGACCAGGGTGGTAATAGTAAGTGGTTGGCTACTCTTGTCCAAACTGCAGCCTTCCCAGTCCTTCTCATCCCGCTTTATTTTCTTCCTTCATCCATAGATCAGGCAACTTCAAACCCACCTTCCATTAAAGTTCTTGCCTTGGTTTACTTCTCTATTGGAGTGCTCTTAGCTGGTGACAACATGATGTACTCTGTCGGACTCTTGTACCTATCTGCCTCTACTTACTCCCTCATATGCGCAACTCAATTAGCTTTTAATGCTGTTTTCTCGTACTTCATCAACTCTCAGAAGTTCACAATGCTGATTCTTAATTCAGTGATTATCGTGTCATTCTCAGCAGCTCTTATTGCTGTTAATGATGATTCCGGAGGGCCAACAGGAGTATCAAAGGGGAAATATATCCTTGGCTTCATCTGTACCATTGGAGCTTCTGCATTGTACTCTCTTTTGCTTTCCCTCATGCAGCTTTCTTTCCAAAAGGTTTTAAAAAGGGAAACGTTTTCTGTGGTTTTGGAGATGCAAATCTATACAGCCCTGGTTGCTAGTTGTGCTGCAACTGTAGGTCTTTTTGCAAGTGGGGAATGGAGGAGTTTACATGGGGAAATGGTAAACTATGGCAAGGGACGAGTTTCTTACGTGATGACCTTAGTTTGGACAGCCGTGGCTTGGCAAGTTTGTTCTGTTGGTGTCGTGGGCTTAATTTTTGTGGTGTCTTCTCTCTTCTCCAATGTAATTAGTACTCTCTCTTTGGCTGTTACTCCTATAGCCGCTGTGATAATCTTCCATGACAAGATGAACGGTGTCAAGGTAATTGCTATGCTTCTGGCTCTTTGGGGTTTCGCCTCTTATGTTTATCAGAACTATCTTGATGACTCTAAAGCAAGAAGAAGACAAGCTGATGCTAGGGAACCACAAAATGATTCCTCTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0005575 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0016020 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUNUT

Protein Analysis

368

Amino Acids

40.19

Weight (kDa)

8.23

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 34 - 350 1.7e-129 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 409
AccIII TCCGGA 1 cut(s) 536
AciI CCGC 2 cut(s) 242, 939
AclI AACGTT 1 cut(s) 668
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 7 cut(s) 108, 359, 377, 443, 589, 611, 910
AfiI CCNNNNNNNGG 4 cut(s) 25, 130, 822, 837
AgsI TTSAA 2 cut(s) 56, 282
AjnI CCWGG 2 cut(s) 174, 705
AluBI AGCT 6 cut(s) 341, 422, 512, 599, 637, 1067
AluI AGCT 6 cut(s) 341, 422, 512, 599, 637, 1067
Alw21I GWGCWC 1 cut(s) 336
Aor13HI TCCGGA 1 cut(s) 536
AoxI GGCC 2 cut(s) 3, 542
ApeKI GCWGC 5 cut(s) 143, 218, 509, 634, 722
AspLEI GCGC 1 cut(s) 410
AspS9I GGNCC 1 cut(s) 542
AsuHPI GGTGA 1 cut(s) 356
BaeI ACNNNNGTAYC 4 cut(s) 359, 392, 537, 570
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 3 cut(s) 109, 876, 1066
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 5 cut(s) 130, 230, 521, 646, 709
BceAI ACGGC 1 cut(s) 815
BciT130I CCWGG 2 cut(s) 176, 707
BfaI CTAG 2 cut(s) 714, 1074
BfmI CTRYAG 3 cut(s) 216, 729, 933
BisI GCNGC 6 cut(s) 144, 219, 510, 635, 723, 939
BlsI GCNGC 6 cut(s) 145, 220, 511, 636, 724, 940
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 2 cut(s) 176, 707
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 1 cut(s) 1080
BmrFI CCNGG 2 cut(s) 176, 707
BmrI ACTGGG 1 cut(s) 221
BmsI GCATC 2 cut(s) 678, 1060
BmuI ACTGGG 1 cut(s) 221
BpiI GAAGAC 3 cut(s) 109, 876, 1066
BsaAI YACGTR 1 cut(s) 808
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 5 cut(s) 175, 309, 574, 705, 831
BsaWI WCCGGW 1 cut(s) 536
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 130, 822, 837
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 95
Bse8I GATNNNNATC 1 cut(s) 692
BseAI TCCGGA 1 cut(s) 536
BseBI CCWGG 2 cut(s) 176, 707
BseDI CCNNGG 5 cut(s) 175, 309, 574, 705, 831
BseGI GGATG 2 cut(s) 237, 260
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 4 cut(s) 25, 130, 822, 837
BseMI GCAATG 1 cut(s) 95
BseMII CTCAG 2 cut(s) 470, 519
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 772
BseXI GCAGC 5 cut(s) 130, 230, 521, 646, 709
BshFI GGCC 2 cut(s) 5, 544
BsiHKAI GWGCWC 1 cut(s) 336
BsiSI CCGG 1 cut(s) 537
BslFI GGGAC 1 cut(s) 807
BslI CCNNNNNNNGG 4 cut(s) 25, 130, 822, 837
BsmFI GGGAC 1 cut(s) 807
BsnI GGCC 2 cut(s) 5, 544
Bsp1286I GDGCHC 1 cut(s) 336
Bsp13I TCCGGA 1 cut(s) 536
Bsp143I GATC 1 cut(s) 268
BspACI CCGC 2 cut(s) 242, 939
BspANI GGCC 2 cut(s) 5, 544
BspCNI CTCAG 2 cut(s) 469, 518
BspEI TCCGGA 1 cut(s) 536
BspLI GGNNCC 1 cut(s) 1080
BspMAI CTGCAG 1 cut(s) 220
BsrDI GCAATG 1 cut(s) 95
BsrI ACTGG 1 cut(s) 227
BssECI CCNNGG 5 cut(s) 175, 309, 574, 705, 831
BssMI GATC 1 cut(s) 268
BssT1I CCWWGG 2 cut(s) 309, 574
Bst2UI CCWGG 2 cut(s) 176, 707
Bst4CI ACNGT 3 cut(s) 49, 730, 971
Bst6I CTCTTC 1 cut(s) 896
BstBAI YACGTR 1 cut(s) 808
BstDEI CTNAG 4 cut(s) 337, 456, 505, 817
BstDSI CCRYGG 1 cut(s) 831
BstF5I GGATG 2 cut(s) 237, 260
BstHHI GCGC 1 cut(s) 410
BstKTI GATC 1 cut(s) 271
BstMBI GATC 1 cut(s) 268
BstMWI GCNNNNNNNGC 4 cut(s) 428, 518, 710, 719
BstNI CCWGG 2 cut(s) 176, 707
BstSCI CCNGG 2 cut(s) 174, 705
BstSFI CTRYAG 3 cut(s) 216, 729, 933
BstV1I GCAGC 5 cut(s) 130, 230, 521, 646, 709
BstV2I GAAGAC 3 cut(s) 109, 876, 1066
BsuRI GGCC 2 cut(s) 5, 544
BtgI CCRYGG 1 cut(s) 831
BtsCI GGATG 2 cut(s) 237, 260
BtsIMutI CAGTG 1 cut(s) 492
CfoI GCGC 1 cut(s) 410
Cfr13I GGNCC 1 cut(s) 542
Csp6I GTAC 7 cut(s) 107, 358, 376, 442, 588, 610, 909
CviAII CATG 5 cut(s) 7, 352, 631, 767, 956
CviQI GTAC 7 cut(s) 107, 358, 376, 442, 588, 610, 909
DdeI CTNAG 4 cut(s) 337, 456, 505, 817
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
DraI TTTAAA 1 cut(s) 657
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 896
EarI CTCTTC 1 cut(s) 896
Eco130I CCWWGG 2 cut(s) 309, 574
EcoRII CCWGG 2 cut(s) 174, 705
EcoT14I CCWWGG 2 cut(s) 309, 574
ErhI CCWWGG 2 cut(s) 309, 574
FaeI CATG 5 cut(s) 10, 355, 634, 770, 959
FaqI GGGAC 1 cut(s) 807
FatI CATG 5 cut(s) 6, 351, 630, 766, 955
FauI CCCGC 1 cut(s) 249
FauNDI CATATG 1 cut(s) 404
Fnu4HI GCNGC 6 cut(s) 144, 219, 510, 635, 723, 939
FokI GGATG 2 cut(s) 224, 247
Fsp4HI GCNGC 6 cut(s) 144, 219, 510, 635, 723, 939
FspBI CTAG 2 cut(s) 714, 1074
FspI TGCGCA 1 cut(s) 409
GlaI GCGC 1 cut(s) 409
GluI GCNGC 6 cut(s) 144, 219, 510, 635, 723, 939
HaeIII GGCC 2 cut(s) 5, 544
HapII CCGG 1 cut(s) 537
HhaI GCGC 1 cut(s) 410
Hin1II CATG 5 cut(s) 10, 355, 634, 770, 959
Hin6I GCGC 1 cut(s) 408
HinP1I GCGC 1 cut(s) 408
HinfI GANTC 7 cut(s) 23, 162, 369, 475, 533, 1043, 1091
HpaII CCGG 1 cut(s) 537
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 4 cut(s) 316, 465, 764, 781
Hpy188I TCNGA 3 cut(s) 368, 459, 1029
Hpy188III TCNNGA 2 cut(s) 537, 1037
Hpy8I GTNNAC 4 cut(s) 316, 465, 764, 781
HpyAV CCTTC 4 cut(s) 232, 242, 267, 300
HpyCH4III ACNGT 3 cut(s) 49, 730, 971
HpyCH4IV ACGT 3 cut(s) 114, 668, 807
HpyCH4V TGCA 6 cut(s) 218, 605, 634, 691, 725, 743
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 518, 710, 719
HpyF3I CTNAG 4 cut(s) 337, 456, 505, 817
HpySE526I ACGT 3 cut(s) 114, 668, 807
Hsp92II CATG 5 cut(s) 10, 355, 634, 770, 959
HspAI GCGC 1 cut(s) 408
Kpn2I TCCGGA 1 cut(s) 536
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 1 cut(s) 596
Lsp1109I GCAGC 5 cut(s) 130, 230, 521, 646, 709
LweI GCATC 2 cut(s) 678, 1060
MaeI CTAG 2 cut(s) 714, 1074
MaeII ACGT 3 cut(s) 114, 668, 807
MaeIII GTNAC 2 cut(s) 344, 925
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 336
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 126, 416, 481, 870, 903, 981
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 363, 1037
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 6 cut(s) 397, 410, 533, 638, 750, 1024
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 536
MscI TGGCCA 1 cut(s) 5
MseI TTAA 8 cut(s) 72, 297, 426, 480, 525, 656, 869, 1102
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 1 cut(s) 537
MspR9I CCNGG 2 cut(s) 176, 707
MvaI CCWGG 2 cut(s) 176, 707
MwoI GCNNNNNNNGC 4 cut(s) 428, 518, 710, 719
NdeI CATATG 1 cut(s) 404
NdeII GATC 1 cut(s) 268
NlaIII CATG 5 cut(s) 10, 355, 634, 770, 959
NlaIV GGNNCC 1 cut(s) 1080
NmuCI GTSAC 1 cut(s) 344
NsbI TGCGCA 1 cut(s) 409
PfeI GAWTC 5 cut(s) 23, 162, 475, 533, 1091
PkrI GCNGC 6 cut(s) 145, 220, 511, 636, 724, 940
PleI GAGTC 2 cut(s) 363, 1037
PpsI GAGTC 2 cut(s) 363, 1037
Ppu21I YACGTR 1 cut(s) 808
Psp1406I AACGTT 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 174, 705
PspGI CCWGG 2 cut(s) 174, 705
PspN4I GGNNCC 1 cut(s) 1080
PspPI GGNCC 1 cut(s) 542
PstI CTGCAG 1 cut(s) 220
RsaI GTAC 7 cut(s) 108, 359, 377, 443, 589, 611, 910
RsaNI GTAC 7 cut(s) 107, 358, 376, 442, 588, 610, 909
SaqAI TTAA 8 cut(s) 72, 297, 426, 480, 525, 656, 869, 1102
SatI GCNGC 6 cut(s) 144, 219, 510, 635, 723, 939
Sau3AI GATC 1 cut(s) 268
Sau96I GGNCC 1 cut(s) 542
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 2 cut(s) 363, 1037
ScrFI CCNGG 2 cut(s) 176, 707
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 2 cut(s) 678, 1060
SfcI CTRYAG 3 cut(s) 216, 729, 933
Sse9I AATT 6 cut(s) 126, 416, 481, 870, 903, 981
SsiI CCGC 2 cut(s) 242, 939
SspMI CTAG 2 cut(s) 714, 1074
StyD4I CCNGG 2 cut(s) 174, 705
StyI CCWWGG 2 cut(s) 309, 574
TaaI ACNGT 3 cut(s) 49, 730, 971
TaiI ACGT 3 cut(s) 117, 671, 810
TaqII GACCGA 1 cut(s) 16
TasI AATT 6 cut(s) 126, 416, 481, 870, 903, 981
TatI WGTACW 3 cut(s) 357, 609, 908
TauI GCSGC 1 cut(s) 941
TfiI GAWTC 5 cut(s) 23, 162, 475, 533, 1091
Tru1I TTAA 8 cut(s) 72, 297, 426, 480, 525, 656, 869, 1102
Tru9I TTAA 8 cut(s) 72, 297, 426, 480, 525, 656, 869, 1102
TscAI CASTG 1 cut(s) 492
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 5 cut(s) 143, 218, 509, 634, 722
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 4 cut(s) 249, 436, 571, 980
TspRI CASTG 1 cut(s) 492
XspI CTAG 2 cut(s) 714, 1074
ZrmI AGTACT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.