pycom02g24520

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0004089
Physical Location & Seq
Reverse (-)
22664044 .. 22666107
2064 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 870 bp
ATGGGAGGAAATACTACTGTAAACAAGAAGTATGAGGCTCACTTGCCGGAAAATGTCAGCAAACCAAAACCAGATTCTTCTATAGAGGAGCGCTTTTATTTTATTAGGAGAAAATATGAGCTGCTAGACTTCTTGAACTCTGATGAACAGATGTCATGTCCTTTCCCACCTAATAAGAAATCAAGCAATTCAACTGTTAGTTCTACCCAAGATAAGAAACAGTATGAGAAACAACCAACGAAACACCGTATTGGTCAGGCATTTCGTAACAGCTGGGGAAGAAAAGACTCTGAACATAAGACCGCAAAGAAGGGCTACTCAATGCCGATGGCAGGTATGGTCGAATTTGTTGGACTAATTAAGGTGAATGTGGTTAAAGGAACCAACCTAGTTGTACGGGATATGGTGACAAGTGACCCTTACGTCATCCTTGCATTGGGTCACCAATCGGTGAAGACGCGTGTCATAAAGAACAACCTTAATCCAGTATGGAATGAGAGCCTGATGCTGTCAATTCCTGATCACATACCTCCTCTAAAAGTGCTGGTATATGACAAAGATACGTTCAAAGCTGATGATTTTATGGGTGAGGCTGAGATTGACATTCAACCTTTGGTGAGTGCTGCCATAGCCTATGAGAAGTCCTCAATCAATGAGCCGATGCAGCTTGGAAAGTGGGTAGCCAGCAAAGAAAATACACTCGTAAAAGATGGTGTCATCAACCTAGTAGACGGGAGAGTGAGACAGCAGATCACTCTCAGGCTGCAGAATGTTGAGAGGGGTATACTGGATCGATCGAGCTTGAATGTGTGCCTCTTAATCAATAGCCCGCATTGGCCTAGAAAGTTAGAAGGAAATGAAATTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
ArfGap C2

Protein Analysis

290

Amino Acids

32.77

Weight (kDa)

9.2

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 119 - 207 2.6e-24 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
Acc36I ACCTGC 1 cut(s) 323
AccI GTMKAC 2 cut(s) 729, 784
AccII CGCG 1 cut(s) 460
AciI CCGC 2 cut(s) 303, 830
AclWI GGATC 1 cut(s) 798
AcsI RAATTY 1 cut(s) 344
AfaI GTAC 1 cut(s) 396
AfeI AGCGCT 1 cut(s) 92
AfiI CCNNNNNNNGG 2 cut(s) 332, 436
AflIII ACRYGT 1 cut(s) 458
AgsI TTSAA 5 cut(s) 136, 192, 568, 608, 805
AjuI GAANNNNNNNTTGG 2 cut(s) 58, 90
AluBI AGCT 5 cut(s) 121, 273, 572, 667, 801
AluI AGCT 5 cut(s) 121, 273, 572, 667, 801
Alw26I GTCTC 1 cut(s) 736
AlwI GGATC 1 cut(s) 798
Aor51HI AGCGCT 1 cut(s) 92
AoxI GGCC 1 cut(s) 836
ApeKI GCWGC 4 cut(s) 121, 623, 664, 763
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 93
AsuHPI GGTGA 6 cut(s) 376, 418, 434, 463, 599, 628
BbsI GAAGAC 1 cut(s) 461
BbvI GCAGC 4 cut(s) 108, 610, 676, 750
BccI CCATC 2 cut(s) 322, 704
BclI TGATCA 1 cut(s) 520
BcoDI GTCTC 1 cut(s) 736
BfaI CTAG 4 cut(s) 125, 389, 725, 840
BfmI CTRYAG 2 cut(s) 81, 764
BfoI RGCGCY 1 cut(s) 94
BfuAI ACCTGC 1 cut(s) 323
BisI GCNGC 4 cut(s) 122, 624, 665, 764
BlsI GCNGC 4 cut(s) 123, 625, 666, 765
BmiI GGNNCC 1 cut(s) 382
BmsI GCATC 2 cut(s) 495, 651
BoxI GACNNNNGTC 1 cut(s) 461
BpiI GAAGAC 1 cut(s) 461
BplI GAGNNNNNCTC 2 cut(s) 629, 661
Bsa29I ATCGAT 1 cut(s) 793
BsaAI YACGTR 1 cut(s) 867
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 436
Bse1I ACTGG 2 cut(s) 485, 792
BseCI ATCGAT 1 cut(s) 793
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 332, 436
BseMII CTCAG 2 cut(s) 585, 772
BseNI ACTGG 2 cut(s) 485, 792
BseRI GAGGAG 2 cut(s) 101, 522
BseXI GCAGC 4 cut(s) 108, 610, 676, 750
BseYI CCCAGC 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 460
Bsh1285I CGRYCG 1 cut(s) 797
BshFI GGCC 1 cut(s) 838
BshVI ATCGAT 1 cut(s) 793
BsiEI CGRYCG 1 cut(s) 797
BsiSI CCGG 1 cut(s) 47
BslI CCNNNNNNNGG 2 cut(s) 332, 436
BsmAI GTCTC 1 cut(s) 736
BsnI GGCC 1 cut(s) 838
Bsp143I GATC 4 cut(s) 520, 750, 790, 794
BspACI CCGC 2 cut(s) 303, 830
BspANI GGCC 1 cut(s) 838
BspCNI CTCAG 2 cut(s) 586, 771
BspDI ATCGAT 1 cut(s) 793
BspFNI CGCG 1 cut(s) 460
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 768
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 1 cut(s) 798
BsrI ACTGG 2 cut(s) 485, 792
BssMI GATC 4 cut(s) 520, 750, 790, 794
BssNAI GTATAC 1 cut(s) 785
Bst1107I GTATAC 1 cut(s) 785
Bst4CI ACNGT 4 cut(s) 19, 196, 222, 248
BstBAI YACGTR 1 cut(s) 867
BstC8I GCNNGC 2 cut(s) 685, 830
BstDEI CTNAG 2 cut(s) 594, 758
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 1 cut(s) 426
BstFNI CGCG 1 cut(s) 460
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 4 cut(s) 523, 753, 793, 797
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 4 cut(s) 520, 750, 790, 794
BstMCI CGRYCG 1 cut(s) 797
BstMWI GCNNNNNNNGC 2 cut(s) 629, 664
BstPAI GACNNNNGTC 1 cut(s) 461
BstPI GGTNACC 1 cut(s) 440
BstSFI CTRYAG 2 cut(s) 81, 764
BstUI CGCG 1 cut(s) 460
BstV1I GCAGC 4 cut(s) 108, 610, 676, 750
BstV2I GAAGAC 1 cut(s) 461
BstZ17I GTATAC 1 cut(s) 785
Bsu15I ATCGAT 1 cut(s) 793
BsuRI GGCC 1 cut(s) 838
BsuTUI ATCGAT 1 cut(s) 793
BtsCI GGATG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 685, 830
CfoI GCGC 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 793
CseI GACGC 1 cut(s) 466
Csp6I GTAC 1 cut(s) 395
CviAII CATG 1 cut(s) 156
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 2 cut(s) 594, 758
DpnI GATC 4 cut(s) 522, 752, 792, 796
DpnII GATC 4 cut(s) 520, 750, 790, 794
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
Eco47III AGCGCT 1 cut(s) 92
Eco91I GGTNACC 1 cut(s) 440
EcoO65I GGTNACC 1 cut(s) 440
FaeI CATG 1 cut(s) 159
FalI AAGNNNNNCTT 2 cut(s) 403, 435
FatI CATG 1 cut(s) 155
FauI CCCGC 1 cut(s) 837
FbaI TGATCA 1 cut(s) 520
FblI GTMKAC 2 cut(s) 729, 784
Fnu4HI GCNGC 4 cut(s) 122, 624, 665, 764
FokI GGATG 1 cut(s) 413
Fsp4HI GCNGC 4 cut(s) 122, 624, 665, 764
FspBI CTAG 4 cut(s) 125, 389, 725, 840
GlaI GCGC 1 cut(s) 92
GluI GCNGC 4 cut(s) 122, 624, 665, 764
GsaI CCCAGC 1 cut(s) 277
HaeII RGCGCY 1 cut(s) 94
HaeIII GGCC 1 cut(s) 838
HapII CCGG 1 cut(s) 47
HgaI GACGC 1 cut(s) 466
HhaI GCGC 1 cut(s) 93
Hin1II CATG 1 cut(s) 159
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HinfI GANTC 2 cut(s) 74, 287
HpaII CCGG 1 cut(s) 47
HphI GGTGA 6 cut(s) 376, 418, 434, 463, 599, 628
Hpy166II GTNNAC 3 cut(s) 22, 730, 785
Hpy188I TCNGA 2 cut(s) 142, 292
Hpy188III TCNNGA 2 cut(s) 133, 518
Hpy8I GTNNAC 3 cut(s) 22, 730, 785
HpyAV CCTTC 2 cut(s) 304, 845
HpyCH4III ACNGT 4 cut(s) 19, 196, 222, 248
HpyCH4IV ACGT 3 cut(s) 423, 563, 866
HpyCH4V TGCA 3 cut(s) 434, 664, 766
HpyF10VI GCNNNNNNNGC 2 cut(s) 629, 664
HpyF3I CTNAG 2 cut(s) 594, 758
HpySE526I ACGT 3 cut(s) 423, 563, 866
Hsp92II CATG 1 cut(s) 159
HspAI GCGC 1 cut(s) 91
Ksp22I TGATCA 1 cut(s) 520
Kzo9I GATC 4 cut(s) 520, 750, 790, 794
LmnI GCTCC 1 cut(s) 88
Lsp1109I GCAGC 4 cut(s) 108, 610, 676, 750
LweI GCATC 2 cut(s) 495, 651
MaeI CTAG 4 cut(s) 125, 389, 725, 840
MaeII ACGT 3 cut(s) 423, 563, 866
MaeIII GTNAC 4 cut(s) 266, 406, 413, 440
MalI GATC 4 cut(s) 522, 752, 792, 796
MboI GATC 4 cut(s) 520, 750, 790, 794
MboII GAAGA 3 cut(s) 69, 291, 466
MluCI AATT 5 cut(s) 187, 344, 357, 513, 861
MluI ACGCGT 1 cut(s) 458
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 331
MnlI CCTC 8 cut(s) 28, 79, 540, 543, 583, 655, 771, 824
MseI TTAA 4 cut(s) 360, 375, 480, 818
MspA1I CMGCKG 1 cut(s) 273
MspI CCGG 1 cut(s) 47
MvnI CGCG 1 cut(s) 460
MwoI GCNNNNNNNGC 2 cut(s) 629, 664
NdeII GATC 4 cut(s) 520, 750, 790, 794
NlaIII CATG 1 cut(s) 159
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 3 cut(s) 406, 413, 440
PfeI GAWTC 1 cut(s) 74
PkrI GCNGC 4 cut(s) 123, 625, 666, 765
Ple19I CGATCG 1 cut(s) 797
PleI GAGTC 1 cut(s) 281
PpsI GAGTC 1 cut(s) 281
Ppu21I YACGTR 1 cut(s) 867
PshAI GACNNNNGTC 1 cut(s) 461
PspEI GGTNACC 1 cut(s) 440
PspFI CCCAGC 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 382
PstI CTGCAG 1 cut(s) 768
PvuI CGATCG 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 273
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
SaqAI TTAA 4 cut(s) 360, 375, 480, 818
SatI GCNGC 4 cut(s) 122, 624, 665, 764
Sau3AI GATC 4 cut(s) 520, 750, 790, 794
SchI GAGTC 1 cut(s) 281
SfaNI GCATC 2 cut(s) 495, 651
SfcI CTRYAG 2 cut(s) 81, 764
Sse9I AATT 5 cut(s) 187, 344, 357, 513, 861
SsiI CCGC 2 cut(s) 303, 830
SspMI CTAG 4 cut(s) 125, 389, 725, 840
TaaI ACNGT 4 cut(s) 19, 196, 222, 248
TaiI ACGT 3 cut(s) 426, 566, 869
TaqI TCGA 3 cut(s) 342, 793, 797
TasI AATT 5 cut(s) 187, 344, 357, 513, 861
TfiI GAWTC 1 cut(s) 74
Tru1I TTAA 4 cut(s) 360, 375, 480, 818
Tru9I TTAA 4 cut(s) 360, 375, 480, 818
TseFI GTSAC 3 cut(s) 406, 413, 440
TseI GCWGC 4 cut(s) 121, 623, 664, 763
Tsp45I GTSAC 3 cut(s) 406, 413, 440
TspDTI ATGAA 1 cut(s) 159
XapI RAATTY 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 729, 784
XspI CTAG 4 cut(s) 125, 389, 725, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.