pycom02g24750

Intron-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0008765
Physical Location & Seq
Reverse (-)
22858699 .. 22860714
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
ATGGATCAGAGTCTCTTTACGAGGTTTGTCCGTTTGGGCATTCCTTATATTGAACTCAATGCCCAGGGTAGAGCCAGGCCAAGTATAGCCAAACTGTACAACTGGAGGAACAGAGATCTGGGTGACCTTCCATATGTAAAGGAGGGTGCCATATTCCATAGAGCAAATTCTGGATTCTCCTATGAATATCAGTTGGTGGATGTGCCAGATTACCATGGCAGAGGTGAGTCTACACCTTCACCTTATTTCTTCCAAAATGTGGGGGAAGCAGAATATGTGGTCAGTGTCTATATCTATATGCGTCTACTTGGGTACCCTGCAAGCAAAATCTCCATATTGACCACTTACAATGGCCAGAAGCTTTTAATCCGCGATGTTATCAACAGACGATGTGCTCCTTATGACTTCGTAGGCCTTCCCAGCAAGGTTACAACAGTTGATAAATTTCAAGGTCAGCAAAATGATTTTACACTGCTATCTCTTGTGCGTACTCGCATTGTGGGTCACCTTCGTGATGTTAGAAGATTGATTGTTGCAATGTCTCGTGCCCGACCGGGTCTTTATGTGTTTTGCCGTCGTTCTTTGTTTGAACAATGCTATGAACTTCAGCCTACATTTCAACGTCTACTTCAGAGACCTGACCACCTTGCTCTTAATTTGAACGAGATTACATCAAACACTGAGCGTCACGTTGAAGAAACTGGTCCTATTCATCTTGTCAGCAGTGTTGATGAGATGATTGGTATCTACCAGCAGCTTTATAAGGTAAAGTTCCATCTGTACATGCCTTATTCAGGTCGAGTAGATGAAATCCTTCCGATGGATACAGATGGGCACCATCCCATGAATACAGATATGGCTTTAACTTCTGATGGGGCACCAACAGATGATGGCCAGCATGGAAGCAATATGGAAGAAGACACTAAAGGCGATGTTGCCACCAATGGGCAGATCACGGAATCATCATTTGAAAACCAGTCAAATGGGGAGACAGATGCTAAAGCTAGCTTGAATGACATAAATATGCCACCTGAAAGCAATTCTGATGAAACAGAAACGGAAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
AAA_11 AAA_12 Aquarius_N

Protein Analysis

356

Amino Acids

40.44

Weight (kDa)

5.28

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_12 PF13087 1 - 193 2.4e-25 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
Acc65I GGTACC 1 cut(s) 312
AccB1I GGYRCC 4 cut(s) 146, 312, 834, 877
AccB7I CCANNNNNTGG 1 cut(s) 259
AccI GTMKAC 3 cut(s) 230, 304, 625
AccII CGCG 1 cut(s) 372
AciI CCGC 1 cut(s) 370
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 352, 892
AcsI RAATTY 2 cut(s) 166, 443
AcuI CTGAAG 2 cut(s) 590, 614
AfaI GTAC 4 cut(s) 98, 314, 490, 782
AfiI CCNNNNNNNGG 4 cut(s) 259, 794, 820, 945
AgsI TTSAA 8 cut(s) 53, 449, 590, 620, 661, 695, 971, 1012
AjnI CCWGG 2 cut(s) 63, 74
AleI CACNNNNGTG 1 cut(s) 510
AluBI AGCT 4 cut(s) 361, 757, 1004, 1008
AluI AGCT 4 cut(s) 361, 757, 1004, 1008
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 4 cut(s) 17, 546, 628, 983
AlwI GGATC 1 cut(s) 12
AoxI GGCC 4 cut(s) 77, 352, 412, 892
ApeKI GCWGC 1 cut(s) 754
ApoI RAATTY 2 cut(s) 166, 443
Asp700I GAANNNNTTC 1 cut(s) 813
Asp718I GGTACC 1 cut(s) 312
AspS9I GGNCC 1 cut(s) 704
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 4 cut(s) 134, 231, 236, 497
AsuNHI GCTAGC 1 cut(s) 1004
AvaII GGWCC 1 cut(s) 704
BaeGI GKGCMC 3 cut(s) 550, 837, 880
BaeI ACNNNNGTAYC 2 cut(s) 296, 329
BalI TGGCCA 2 cut(s) 354, 894
BanI GGYRCC 4 cut(s) 146, 312, 834, 877
BauI CACGAG 1 cut(s) 543
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 1 cut(s) 397
BbvI GCAGC 1 cut(s) 766
BccI CCATC 6 cut(s) 783, 814, 824, 846, 866, 884
BceAI ACGGC 1 cut(s) 558
BciT130I CCWGG 2 cut(s) 65, 76
BciVI GTATCC 1 cut(s) 817
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 4 cut(s) 17, 546, 628, 983
BfaI CTAG 1 cut(s) 1005
BfuI GTATCC 1 cut(s) 817
BglII AGATCT 1 cut(s) 115
BisI GCNGC 1 cut(s) 755
BlsI GCNGC 1 cut(s) 756
Bme1390I CCNGG 3 cut(s) 65, 76, 555
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 4 cut(s) 148, 314, 836, 879
BmrFI CCNGG 3 cut(s) 65, 76, 555
BmsI GCATC 1 cut(s) 985
BmtI GCTAGC 1 cut(s) 1008
BpiI GAAGAC 1 cut(s) 924
BpmI CTGGAG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 555
BsaBI GATNNNNATC 1 cut(s) 743
BsaI GGTCTC 1 cut(s) 628
BsaJI CCNNGG 3 cut(s) 63, 64, 214
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 794, 820, 945
Bse1I ACTGG 3 cut(s) 107, 706, 976
Bse3DI GCAATG 1 cut(s) 543
Bse8I GATNNNNATC 1 cut(s) 743
BseBI CCWGG 2 cut(s) 65, 76
BseDI CCNNGG 3 cut(s) 63, 64, 214
BseGI GGATG 2 cut(s) 205, 838
BseJI GATNNNNATC 1 cut(s) 743
BseLI CCNNNNNNNGG 4 cut(s) 259, 794, 820, 945
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 3 cut(s) 107, 706, 976
BseSI GKGCMC 3 cut(s) 550, 837, 880
BseXI GCAGC 1 cut(s) 766
BseYI CCCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 372
Bsh1285I CGRYCG 1 cut(s) 554
BshFI GGCC 4 cut(s) 79, 354, 414, 894
BshNI GGYRCC 4 cut(s) 146, 312, 834, 877
BsiEI CGRYCG 1 cut(s) 554
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 1 cut(s) 554
BslI CCNNNNNNNGG 4 cut(s) 259, 794, 820, 945
BsmAI GTCTC 4 cut(s) 17, 546, 628, 983
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 4 cut(s) 79, 354, 414, 894
Bso31I GGTCTC 1 cut(s) 628
Bsp1286I GDGCHC 4 cut(s) 397, 550, 837, 880
Bsp1407I TGTACA 2 cut(s) 96, 780
Bsp143I GATC 3 cut(s) 4, 115, 951
Bsp19I CCATGG 1 cut(s) 214
BspACI CCGC 1 cut(s) 370
BspANI GGCC 4 cut(s) 79, 354, 414, 894
BspCNI CTCAG 1 cut(s) 673
BspFNI CGCG 1 cut(s) 372
BspLI GGNNCC 4 cut(s) 148, 314, 836, 879
BspOI GCTAGC 1 cut(s) 1008
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 4 cut(s) 146, 312, 834, 877
BspTNI GGTCTC 1 cut(s) 628
BsrDI GCAATG 1 cut(s) 543
BsrGI TGTACA 2 cut(s) 96, 780
BsrI ACTGG 3 cut(s) 107, 706, 976
BssECI CCNNGG 3 cut(s) 63, 64, 214
BssMI GATC 3 cut(s) 4, 115, 951
BssSI CACGAG 1 cut(s) 543
BssT1I CCWWGG 1 cut(s) 214
Bst2BI CACGAG 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 65, 76
Bst4CI ACNGT 2 cut(s) 96, 436
Bst6I CTCTTC 1 cut(s) 1056
BstAUI TGTACA 2 cut(s) 96, 780
BstC8I GCNNGC 3 cut(s) 322, 896, 1006
BstDEI CTNAG 1 cut(s) 681
BstDSI CCRYGG 1 cut(s) 214
BstEII GGTNACC 2 cut(s) 122, 503
BstENI CCTNNNNNAGG 1 cut(s) 792
BstF5I GGATG 2 cut(s) 205, 838
BstFNI CGCG 1 cut(s) 372
BstKTI GATC 3 cut(s) 7, 118, 954
BstMAI GTCTC 4 cut(s) 17, 546, 628, 983
BstMBI GATC 3 cut(s) 4, 115, 951
BstMCI CGRYCG 1 cut(s) 554
BstMWI GCNNNNNNNGC 1 cut(s) 420
BstNI CCWGG 2 cut(s) 65, 76
BstNSI RCATGY 1 cut(s) 787
BstPI GGTNACC 2 cut(s) 122, 503
BstSCI CCNGG 3 cut(s) 63, 74, 553
BstSLI GKGCMC 3 cut(s) 550, 837, 880
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 1 cut(s) 766
BstV2I GAAGAC 1 cut(s) 924
BstX2I RGATCY 1 cut(s) 115
BstXI CCANNNNNNTGG 1 cut(s) 983
BstYI RGATCY 1 cut(s) 115
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 4 cut(s) 79, 354, 414, 894
BtgI CCRYGG 1 cut(s) 214
BtgZI GCGATG 2 cut(s) 387, 945
BtsCI GGATG 2 cut(s) 205, 838
BtsI GCAGTG 2 cut(s) 470, 730
BtsIMutI CAGTG 4 cut(s) 289, 470, 678, 730
Cac8I GCNNGC 3 cut(s) 322, 896, 1006
Cfr13I GGNCC 1 cut(s) 704
CseI GACGC 2 cut(s) 290, 674
Csp6I GTAC 4 cut(s) 97, 313, 489, 781
CviAII CATG 4 cut(s) 215, 784, 844, 899
CviQI GTAC 4 cut(s) 97, 313, 489, 781
DdeI CTNAG 1 cut(s) 681
DpnI GATC 3 cut(s) 6, 117, 953
DpnII GATC 3 cut(s) 4, 115, 951
EaeI YGGCCR 2 cut(s) 352, 892
Eam1104I CTCTTC 1 cut(s) 1056
EarI CTCTTC 1 cut(s) 1056
Eco130I CCWWGG 1 cut(s) 214
Eco147I AGGCCT 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 628
Eco47I GGWCC 1 cut(s) 704
Eco57I CTGAAG 2 cut(s) 590, 614
Eco91I GGTNACC 2 cut(s) 122, 503
EcoNI CCTNNNNNAGG 1 cut(s) 792
EcoO65I GGTNACC 2 cut(s) 122, 503
EcoRII CCWGG 2 cut(s) 63, 74
EcoT14I CCWWGG 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 4 cut(s) 218, 787, 847, 902
FatI CATG 4 cut(s) 214, 783, 843, 898
FauNDI CATATG 1 cut(s) 133
FblI GTMKAC 3 cut(s) 230, 304, 625
Fnu4HI GCNGC 1 cut(s) 755
FokI GGATG 2 cut(s) 212, 825
Fsp4HI GCNGC 1 cut(s) 755
FspBI CTAG 1 cut(s) 1005
GluI GCNGC 1 cut(s) 755
GsaI CCCAGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 4 cut(s) 79, 354, 414, 894
HapII CCGG 1 cut(s) 554
HgaI GACGC 2 cut(s) 290, 674
Hin1II CATG 4 cut(s) 218, 787, 847, 902
HindIII AAGCTT 1 cut(s) 359
HinfI GANTC 4 cut(s) 10, 174, 227, 959
HpaII CCGG 1 cut(s) 554
HphI GGTGA 4 cut(s) 134, 231, 236, 497
Hpy166II GTNNAC 3 cut(s) 231, 305, 626
Hpy188I TCNGA 5 cut(s) 9, 633, 819, 871, 1045
Hpy188III TCNNGA 2 cut(s) 171, 512
Hpy8I GTNNAC 3 cut(s) 231, 305, 626
Hpy99I CGWCG 1 cut(s) 579
HpyAV CCTTC 5 cut(s) 137, 246, 425, 518, 824
HpyCH4III ACNGT 2 cut(s) 96, 436
HpyCH4IV ACGT 2 cut(s) 622, 690
HpyCH4V TGCA 2 cut(s) 320, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 420
HpyF3I CTNAG 1 cut(s) 681
HpySE526I ACGT 2 cut(s) 622, 690
Hsp92II CATG 4 cut(s) 218, 787, 847, 902
KpnI GGTACC 1 cut(s) 316
Kzo9I GATC 3 cut(s) 4, 115, 951
LmnI GCTCC 1 cut(s) 400
Lsp1109I GCAGC 1 cut(s) 766
LweI GCATC 1 cut(s) 985
MaeI CTAG 1 cut(s) 1005
MaeII ACGT 2 cut(s) 622, 690
MaeIII GTNAC 4 cut(s) 122, 427, 503, 686
MalI GATC 3 cut(s) 6, 117, 953
MboI GATC 3 cut(s) 4, 115, 951
MboII GAAGA 5 cut(s) 241, 534, 707, 926, 929
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 4 cut(s) 397, 550, 837, 880
MlsI TGGCCA 2 cut(s) 354, 894
MluCI AATT 4 cut(s) 166, 443, 655, 1039
MluNI TGGCCA 2 cut(s) 354, 894
MlyI GAGTC 2 cut(s) 19, 236
MnlI CCTC 4 cut(s) 15, 99, 136, 215
Mox20I TGGCCA 2 cut(s) 354, 894
MroXI GAANNNNTTC 1 cut(s) 813
MscI TGGCCA 2 cut(s) 354, 894
MseI TTAA 3 cut(s) 365, 654, 863
MslI CAYNNNNRTG 2 cut(s) 510, 1022
Msp20I TGGCCA 2 cut(s) 354, 894
MspI CCGG 1 cut(s) 554
MspR9I CCNGG 3 cut(s) 65, 76, 555
Mva1269I GAATGC 1 cut(s) 39
MvaI CCWGG 2 cut(s) 65, 76
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 1 cut(s) 420
NciI CCSGG 1 cut(s) 555
NcoI CCATGG 1 cut(s) 214
NdeI CATATG 1 cut(s) 133
NdeII GATC 3 cut(s) 4, 115, 951
NheI GCTAGC 1 cut(s) 1004
NlaIII CATG 4 cut(s) 218, 787, 847, 902
NlaIV GGNNCC 4 cut(s) 148, 314, 836, 879
NmuCI GTSAC 3 cut(s) 122, 503, 686
NspI RCATGY 1 cut(s) 787
OliI CACNNNNGTG 1 cut(s) 510
PasI CCCWGGG 1 cut(s) 64
PceI AGGCCT 1 cut(s) 414
PctI GAATGC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 2 cut(s) 174, 959
PflFI GACNNNGTC 1 cut(s) 555
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 1 cut(s) 756
PleI GAGTC 2 cut(s) 18, 235
PpsI GAGTC 2 cut(s) 18, 235
PsiI TTATAA 1 cut(s) 762
Psp6I CCWGG 2 cut(s) 63, 74
PspEI GGTNACC 2 cut(s) 122, 503
PspFI CCCAGC 1 cut(s) 419
PspGI CCWGG 2 cut(s) 63, 74
PspN4I GGNNCC 4 cut(s) 148, 314, 836, 879
PspPI GGNCC 1 cut(s) 704
PsuI RGATCY 1 cut(s) 115
PsyI GACNNNGTC 1 cut(s) 555
RsaI GTAC 4 cut(s) 98, 314, 490, 782
RsaNI GTAC 4 cut(s) 97, 313, 489, 781
RseI CAYNNNNRTG 2 cut(s) 510, 1022
SaqAI TTAA 3 cut(s) 365, 654, 863
SatI GCNGC 1 cut(s) 755
Sau3AI GATC 3 cut(s) 4, 115, 951
Sau96I GGNCC 1 cut(s) 704
SchI GAGTC 2 cut(s) 19, 236
ScrFI CCNGG 3 cut(s) 65, 76, 555
SduI GDGCHC 4 cut(s) 397, 550, 837, 880
SfaNI GCATC 1 cut(s) 985
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 2 cut(s) 510, 1022
Sse9I AATT 4 cut(s) 166, 443, 655, 1039
SseBI AGGCCT 1 cut(s) 414
SsiI CCGC 1 cut(s) 370
SspMI CTAG 1 cut(s) 1005
StuI AGGCCT 1 cut(s) 414
StyD4I CCNGG 3 cut(s) 63, 74, 553
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 2 cut(s) 96, 436
TaiI ACGT 2 cut(s) 625, 693
TaqI TCGA 1 cut(s) 799
TasI AATT 4 cut(s) 166, 443, 655, 1039
TatI WGTACW 2 cut(s) 96, 780
TfiI GAWTC 2 cut(s) 174, 959
Tru1I TTAA 3 cut(s) 365, 654, 863
Tru9I TTAA 3 cut(s) 365, 654, 863
TscAI CASTG 4 cut(s) 289, 477, 685, 730
TseFI GTSAC 3 cut(s) 122, 503, 686
TseI GCWGC 1 cut(s) 754
Tsp45I GTSAC 3 cut(s) 122, 503, 686
TspDTI ATGAA 6 cut(s) 198, 615, 701, 822, 860, 1062
TspGWI ACGGA 2 cut(s) 20, 971
TspRI CASTG 4 cut(s) 289, 477, 685, 730
Tth111I GACNNNGTC 1 cut(s) 555
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 704
XagI CCTNNNNNAGG 1 cut(s) 792
XapI RAATTY 2 cut(s) 166, 443
XceI RCATGY 1 cut(s) 787
XmiI GTMKAC 3 cut(s) 230, 304, 625
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 1 cut(s) 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.