pycom02g25030

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007852
Physical Location & Seq
Reverse (-)
23069032 .. 23070423
1392 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
ATGCCACCTCAGTCCCCACCACCTAAATTCCAAAACTTTACCTTCTTCCACGGCCACCGCAAGCCCGCACAGAACCGCCCCACCGTCCGCGGCAGCCTGTTCTCCAACCCCGTCTCTCACCCAAATCCCAAACACCCCACCACCGCCCCTCAATCCCGACCCTTCGATGTCTCCAAGTGGGACCCACACCTGCGCCATTCCTTGCCCTCCACTTCATCGCCCAACCCCGACGACACGACCCTCCAGTCGTCTCGGAGCTCCGGAAGCTTCGCCGGGTCACACCCGACCTCGTTGCCAAGGTGCTCAAGGTCCATGTCGACCCCGTTTCAGCCTCCAAGTTCTTCCACTGGGCAGGGCAAACTGCCCAGCGAGAAACAGTTTGAGATTCTCATCAGAATGCACTCTGATGCCAATAGAGGTCTTAGGATTTATTATGTGTATAAAAAAATGAAGAAAGTTGGGGTTAAACCTAGGGTTTTCTTGTACAATAGGATCATGGATGCATTGGCGAAAACCGGTTATTTGGATTTGGCTTTATCGGTTTACAAAGATTTGCGTGGTGATGGATTGGTGGAGGAGAGTGTTACTTTCATGATCTTAATAAAGGGTATGTGTAAAATGGGAAGAATTGATGAAATGTTGCAGCTTTTGGAGAGAATGAGGGTGAAATTGTGCAAACCGGACGTGTTTGCATACACAGCAATGATTAAGGTTTTGCTTTCGGAGGGAAACTTGGATGGGTGTTTGAGGGTTTGGGAGGACATGACGAGAGATAGGGTTGAGGCAGACGCTATGGCATATGAAACTCTGGTTACAGGCGTGACAAGTGGGCCCATAGGATTCAAGTATTCTCTTACCATTCTCCATGCATGTAAATCAAATAATGCTGAGAAGGTGGTTGAGGTGCTAAACAAGATGCTGCAGCAGGGTTGTCTTCCAGATGATGTTGTATACTCAGCTATAATCTCTGGCATGTGTAAGCATGGCACAATAGAGGAGGCAAGAAAAATATTTTCAAATGTGAAAGAGCGCTATATTTTAACAGAAGCCAACATGATTGTGTATAATGAAGTATTAATTGAACACATGAAGAAGAAGACCGCAGATCTGGTGGTGTCTGGGTTGAAGTTTTTTGGATTGGAAAAAAAGTTGAAAGCAAAGGGTTCTAAGCTGCTGTCAGGATGA

Protein Analysis

395

Amino Acids

44.13

Weight (kDa)

9.57

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 113 - 227 1.7e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 165 - 272 5.7e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 194 - 239 6.7e-12 PPR repeat family
PPR_1 PF12854 194 - 221 1.6e-06 PPR repeat
PPR_2 PF13041 227 - 273 2.9e-08 PPR repeat family
PPR_2 PF13041 287 - 327 1.8e-08 PPR repeat family
PPR_1 PF12854 310 - 339 3.3e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 198
AasI GACNNNNNNGTC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 198
AccI GTMKAC 2 cut(s) 317, 951
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 7 cut(s) 58, 66, 76, 88, 90, 144, 1101
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 1 cut(s) 52
AcsI RAATTY 1 cut(s) 26
AfaI GTAC 1 cut(s) 485
AfeI AGCGCT 1 cut(s) 1031
AflIII ACRYGT 1 cut(s) 684
AgeI ACCGGT 1 cut(s) 515
AgsI TTSAA 5 cut(s) 844, 1017, 1082, 1126, 1153
AjiI CACGTC 1 cut(s) 685
AluBI AGCT 5 cut(s) 258, 267, 646, 959, 1171
AluI AGCT 5 cut(s) 258, 267, 646, 959, 1171
Alw21I GWGCWC 2 cut(s) 260, 305
Alw26I GTCTC 3 cut(s) 118, 175, 255
AlwI GGATC 1 cut(s) 500
Aor13HI TCCGGA 1 cut(s) 260
Aor51HI AGCGCT 1 cut(s) 1031
AoxI GGCC 2 cut(s) 52, 830
ApaI GGGCCC 1 cut(s) 834
ApeKI GCWGC 5 cut(s) 93, 643, 919, 922, 1171
ApoI RAATTY 1 cut(s) 26
AseI ATTAAT 1 cut(s) 1076
AsiGI ACCGGT 1 cut(s) 515
AspA2I CCTAGG 1 cut(s) 470
AspLEI GCGC 2 cut(s) 195, 1032
AspS9I GGNCC 4 cut(s) 181, 309, 830, 831
AsuC2I CCSGG 1 cut(s) 274
AsuHPI GGTGA 3 cut(s) 110, 572, 676
AvaII GGWCC 2 cut(s) 181, 309
AvrII CCTAGG 1 cut(s) 470
BaeGI GKGCMC 1 cut(s) 834
BanII GRGCYC 2 cut(s) 260, 834
BbsI GAAGAC 2 cut(s) 926, 1103
Bbv12I GWGCWC 2 cut(s) 260, 305
BbvI GCAGC 5 cut(s) 105, 655, 906, 934, 1158
BccI CCATC 2 cut(s) 557, 731
BceAI ACGGC 1 cut(s) 67
BcgI CGANNNNNNTGC 2 cut(s) 274, 308
BcnI CCSGG 1 cut(s) 274
BcoDI GTCTC 3 cut(s) 118, 175, 255
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 920
BfoI RGCGCY 1 cut(s) 1033
BfuAI ACCTGC 1 cut(s) 198
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 6 cut(s) 91, 94, 644, 920, 923, 1172
BlnI CCTAGG 1 cut(s) 470
BlsI GCNGC 6 cut(s) 92, 95, 645, 921, 924, 1173
Bme1390I CCNGG 1 cut(s) 274
Bme18I GGWCC 2 cut(s) 181, 309
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 4 cut(s) 181, 309, 830, 831
BmiI GGNNCC 3 cut(s) 182, 183, 832
BmrFI CCNGG 1 cut(s) 274
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 3 cut(s) 397, 490, 906
BmuI ACTGGG 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 926, 1103
BpmI CTGGAG 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 289
BpuMI CCSGG 1 cut(s) 274
BsaBI GATNNNNATC 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 49, 88, 296, 470
BsaWI WCCGGW 3 cut(s) 260, 515, 679
Bse118I RCCGGY 1 cut(s) 515
Bse1I ACTGG 2 cut(s) 244, 352
Bse3DI GCAATG 1 cut(s) 708
Bse8I GATNNNNATC 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 260
BseDI CCNNGG 4 cut(s) 49, 88, 296, 470
BseGI GGATG 2 cut(s) 505, 742
BseJI GATNNNNATC 1 cut(s) 389
BseMI GCAATG 1 cut(s) 708
BseMII CTCAG 3 cut(s) 23, 879, 969
BseNI ACTGG 2 cut(s) 244, 352
BseRI GAGGAG 2 cut(s) 590, 1010
BseSI GKGCMC 1 cut(s) 834
BseXI GCAGC 5 cut(s) 105, 655, 906, 934, 1158
BseYI CCCAGC 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 2 cut(s) 54, 832
BshTI ACCGGT 1 cut(s) 515
BsiHKAI GWGCWC 2 cut(s) 260, 305
BsiSI CCGG 4 cut(s) 261, 273, 516, 680
BslFI GGGAC 1 cut(s) 194
BsmAI GTCTC 3 cut(s) 118, 175, 255
BsmBI CGTCTC 2 cut(s) 118, 255
BsmFI GGGAC 1 cut(s) 194
BsmI GAATGC 1 cut(s) 402
BsnI GGCC 2 cut(s) 54, 832
Bsp120I GGGCCC 1 cut(s) 830
Bsp1286I GDGCHC 3 cut(s) 260, 305, 834
Bsp13I TCCGGA 1 cut(s) 260
Bsp1407I TGTACA 1 cut(s) 483
Bsp143I GATC 3 cut(s) 492, 594, 1105
BspACI CCGC 7 cut(s) 58, 66, 76, 88, 90, 144, 1101
BspANI GGCC 2 cut(s) 54, 832
BspCNI CTCAG 3 cut(s) 22, 880, 968
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 1 cut(s) 90
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 3 cut(s) 182, 183, 832
BspMAI CTGCAG 1 cut(s) 924
BspMI ACCTGC 1 cut(s) 198
BspPI GGATC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 515
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 2 cut(s) 244, 352
BssAI RCCGGY 1 cut(s) 515
BssECI CCNNGG 4 cut(s) 49, 88, 296, 470
BssMI GATC 3 cut(s) 492, 594, 1105
BssNAI GTATAC 1 cut(s) 952
BssT1I CCWWGG 2 cut(s) 296, 470
Bst1107I GTATAC 1 cut(s) 952
Bst4CI ACNGT 2 cut(s) 85, 378
BstAUI TGTACA 1 cut(s) 483
BstC8I GCNNGC 2 cut(s) 62, 66
BstDEI CTNAG 5 cut(s) 9, 422, 888, 955, 1167
BstDSI CCRYGG 2 cut(s) 49, 88
BstF5I GGATG 2 cut(s) 505, 742
BstFNI CGCG 1 cut(s) 90
BstH2I RGCGCY 1 cut(s) 1033
BstHHI GCGC 2 cut(s) 195, 1032
BstKTI GATC 3 cut(s) 495, 597, 1108
BstMAI GTCTC 3 cut(s) 118, 175, 255
BstMBI GATC 3 cut(s) 492, 594, 1105
BstMWI GCNNNNNNNGC 2 cut(s) 264, 698
BstNSI RCATGY 2 cut(s) 873, 976
BstSCI CCNGG 1 cut(s) 272
BstSFI CTRYAG 1 cut(s) 920
BstSLI GKGCMC 1 cut(s) 834
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 5 cut(s) 105, 655, 906, 934, 1158
BstV2I GAAGAC 2 cut(s) 926, 1103
BstX2I RGATCY 1 cut(s) 1105
BstYI RGATCY 1 cut(s) 1105
BstZ17I GTATAC 1 cut(s) 952
BsuRI GGCC 2 cut(s) 54, 832
BtgI CCRYGG 2 cut(s) 49, 88
BtgZI GCGATG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 685
BtsCI GGATG 2 cut(s) 505, 742
BtsIMutI CAGTG 1 cut(s) 345
BveI ACCTGC 1 cut(s) 198
Cac8I GCNNGC 2 cut(s) 62, 66
CciI TCATGA 1 cut(s) 591
CfoI GCGC 2 cut(s) 195, 1032
Cfr10I RCCGGY 1 cut(s) 515
Cfr13I GGNCC 4 cut(s) 181, 309, 830, 831
Cfr42I CCGCGG 1 cut(s) 91
CseI GACGC 1 cut(s) 797
Csp6I GTAC 1 cut(s) 484
CspAI ACCGGT 1 cut(s) 515
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 5 cut(s) 9, 422, 888, 955, 1167
DpnI GATC 3 cut(s) 494, 596, 1107
DpnII GATC 3 cut(s) 492, 594, 1105
DrdI GACNNNNNNGTC 1 cut(s) 244
DseDI GACNNNNNNGTC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 296, 470
Eco24I GRGCYC 2 cut(s) 260, 834
Eco47I GGWCC 2 cut(s) 181, 309
Eco47III AGCGCT 1 cut(s) 1031
Eco53kI GAGCTC 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 181
EcoT14I CCWWGG 2 cut(s) 296, 470
EcoT22I ATGCAT 2 cut(s) 505, 871
EcoT38I GRGCYC 2 cut(s) 260, 834
ErhI CCWWGG 2 cut(s) 296, 470
Esp3I CGTCTC 2 cut(s) 118, 255
FaqI GGGAC 1 cut(s) 194
FauI CCCGC 1 cut(s) 73
FauNDI CATATG 1 cut(s) 799
FblI GTMKAC 2 cut(s) 317, 951
Fnu4HI GCNGC 6 cut(s) 91, 94, 644, 920, 923, 1172
FokI GGATG 2 cut(s) 512, 749
FriOI GRGCYC 2 cut(s) 260, 834
Fsp4HI GCNGC 6 cut(s) 91, 94, 644, 920, 923, 1172
FspBI CTAG 1 cut(s) 471
GlaI GCGC 2 cut(s) 194, 1031
GluI GCNGC 6 cut(s) 91, 94, 644, 920, 923, 1172
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 227
HaeII RGCGCY 1 cut(s) 1033
HaeIII GGCC 2 cut(s) 54, 832
HapII CCGG 4 cut(s) 261, 273, 516, 680
HgaI GACGC 1 cut(s) 797
HhaI GCGC 2 cut(s) 195, 1032
Hin6I GCGC 2 cut(s) 193, 1030
HinP1I GCGC 2 cut(s) 193, 1030
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 2 cut(s) 385, 840
HpaII CCGG 4 cut(s) 261, 273, 516, 680
HphI GGTGA 3 cut(s) 110, 572, 676
Hpy166II GTNNAC 3 cut(s) 318, 544, 952
Hpy188I TCNGA 4 cut(s) 255, 395, 406, 724
Hpy188III TCNNGA 5 cut(s) 156, 261, 592, 938, 1179
Hpy8I GTNNAC 3 cut(s) 318, 544, 952
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 3 cut(s) 52, 172, 886
HpyCH4III ACNGT 2 cut(s) 85, 378
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 7 cut(s) 400, 503, 643, 675, 692, 869, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 698
HpyF3I CTNAG 5 cut(s) 9, 422, 888, 955, 1167
HpySE526I ACGT 1 cut(s) 684
HspAI GCGC 2 cut(s) 193, 1030
KflI GGGWCCC 1 cut(s) 181
Kpn2I TCCGGA 1 cut(s) 260
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 3 cut(s) 492, 594, 1105
LmnI GCTCC 2 cut(s) 255, 263
Lsp1109I GCAGC 5 cut(s) 105, 655, 906, 934, 1158
LweI GCATC 3 cut(s) 397, 490, 906
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 4 cut(s) 276, 583, 811, 820
MalI GATC 3 cut(s) 494, 596, 1107
MboI GATC 3 cut(s) 492, 594, 1105
MboII GAAGA 8 cut(s) 37, 333, 463, 636, 926, 1102, 1105, 1108
MflI RGATCY 1 cut(s) 1105
MhlI GDGCHC 3 cut(s) 260, 305, 834
MluCI AATT 4 cut(s) 26, 627, 668, 1077
MmeI TCCRAC 1 cut(s) 129
Mph1103I ATGCAT 2 cut(s) 505, 871
MroI TCCGGA 1 cut(s) 260
MseI TTAA 5 cut(s) 465, 599, 708, 1040, 1076
MslI CAYNNNNRTG 4 cut(s) 395, 405, 701, 1058
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 4 cut(s) 261, 273, 516, 680
MspR9I CCNGG 1 cut(s) 274
Mva1269I GAATGC 1 cut(s) 402
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 2 cut(s) 264, 698
NciI CCSGG 1 cut(s) 274
NdeI CATATG 1 cut(s) 799
NdeII GATC 3 cut(s) 492, 594, 1105
NlaIV GGNNCC 3 cut(s) 182, 183, 832
NmuCI GTSAC 2 cut(s) 276, 820
NsiI ATGCAT 2 cut(s) 505, 871
NspI RCATGY 2 cut(s) 873, 976
PagI TCATGA 1 cut(s) 591
PaqCI CACCTGC 1 cut(s) 198
PctI GAATGC 1 cut(s) 402
PfeI GAWTC 2 cut(s) 385, 840
PinAI ACCGGT 1 cut(s) 515
PkrI GCNGC 6 cut(s) 92, 95, 645, 921, 924, 1173
PpuMI RGGWCCY 1 cut(s) 181
PshBI ATTAAT 1 cut(s) 1076
Psp124BI GAGCTC 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 365
PspN4I GGNNCC 3 cut(s) 182, 183, 832
PspOMI GGGCCC 1 cut(s) 830
PspPI GGNCC 4 cut(s) 181, 309, 830, 831
PspPPI RGGWCCY 1 cut(s) 181
PstI CTGCAG 1 cut(s) 924
PsuI RGATCY 1 cut(s) 1105
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 4 cut(s) 395, 405, 701, 1058
SacI GAGCTC 1 cut(s) 260
SacII CCGCGG 1 cut(s) 91
SalI GTCGAC 1 cut(s) 316
SaqAI TTAA 5 cut(s) 465, 599, 708, 1040, 1076
SatI GCNGC 6 cut(s) 91, 94, 644, 920, 923, 1172
Sau3AI GATC 3 cut(s) 492, 594, 1105
Sau96I GGNCC 4 cut(s) 181, 309, 830, 831
ScrFI CCNGG 1 cut(s) 274
SduI GDGCHC 3 cut(s) 260, 305, 834
SfaNI GCATC 3 cut(s) 397, 490, 906
SfcI CTRYAG 1 cut(s) 920
Sfr303I CCGCGG 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 91
SinI GGWCC 2 cut(s) 181, 309
SmiMI CAYNNNNRTG 4 cut(s) 395, 405, 701, 1058
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 4 cut(s) 26, 627, 668, 1077
SsiI CCGC 7 cut(s) 58, 66, 76, 88, 90, 144, 1101
SspI AATATT 1 cut(s) 1011
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 272
StyI CCWWGG 2 cut(s) 296, 470
TaaI ACNGT 2 cut(s) 85, 378
TaiI ACGT 1 cut(s) 687
TaqI TCGA 2 cut(s) 165, 317
TasI AATT 4 cut(s) 26, 627, 668, 1077
TatI WGTACW 1 cut(s) 483
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 2 cut(s) 385, 840
Tru1I TTAA 5 cut(s) 465, 599, 708, 1040, 1076
Tru9I TTAA 5 cut(s) 465, 599, 708, 1040, 1076
TscAI CASTG 1 cut(s) 352
TseFI GTSAC 2 cut(s) 276, 820
TseI GCWGC 5 cut(s) 93, 643, 919, 922, 1171
Tsp45I GTSAC 2 cut(s) 276, 820
TspDTI ATGAA 7 cut(s) 204, 464, 580, 648, 816, 1083, 1103
TspRI CASTG 1 cut(s) 352
VpaK11BI GGWCC 2 cut(s) 181, 309
VspI ATTAAT 1 cut(s) 1076
XapI RAATTY 1 cut(s) 26
XceI RCATGY 2 cut(s) 873, 976
XmaJI CCTAGG 1 cut(s) 470
XmiI GTMKAC 2 cut(s) 317, 951
XspI CTAG 1 cut(s) 471
Zsp2I ATGCAT 2 cut(s) 505, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.