pycom02g25170

Structure-specific endonuclease subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009332
Physical Location & Seq
Forward (+)
23210805 .. 23212978
2174 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
ATGGCACCGGATAAAACATACGAGCTGCCAAACGAGGCCTATGGCATATTTAAATGGAACAGTTCAAAAACGTTCAGACTTTTAGGCCGATCGAGCAGAGAATTGATCTGCTCACCTGAAATTATGCGACGGCTCTCGAGCACATTCCCTTCCGTCAAGTTCCAGAACCCTAACCCCCCAAAATTATCATCATCCTCCTCGCTCTCCCTGTCGATCAAATCAGAAGCATCCTCGAGCTCTCCGTCCAAATCAAAATCAAAACCAAAATCTTGGTCTGTGTATCTCATTCTTTCCACTAATTCCCCAATCAAAACCTACGTCGGCGTCACCACCGATTTCTCTCGCCGTTTGAAACAACATAATGGCGAGCTCAAAGGTGGTGCAAAAGCATCTAGTGCTGGAAGACCATGGATTTGTGCTTGCCTAATACATGGTTTTAAGGGCCAAAGTGAAGCTTGCGAGTTCGAATCAAAATGGAAAACTTTATCAAGGCGTTTGCCTCGGAAAAGAAAGAAACACGACGAGGAGGAGCAAGCAAATGATTTGTCACCTCCATTGCTACAACACAGGCAAGCAGCTCTGGATAGAGTTAAAGGTATACTTGATTGCACCAACTTAGAAATTGACTGGAAACTGGACCCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
GIY-YIG

Protein Analysis

216

Amino Acids

24.16

Weight (kDa)

9.77

Isoelectric Point (pI)

63.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIY-YIG PF01541 91 - 165 1.4e-14 GIY-YIG catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 598
AclI AACGTT 1 cut(s) 71
AcyI GRCGYC 1 cut(s) 324
AgsI TTSAA 2 cut(s) 66, 352
AluBI AGCT 5 cut(s) 25, 237, 370, 455, 578
AluI AGCT 5 cut(s) 25, 237, 370, 455, 578
Alw21I GWGCWC 3 cut(s) 143, 239, 372
Ama87I CYCGRG 2 cut(s) 136, 232
AoxI GGCC 3 cut(s) 36, 85, 442
ApeKI GCWGC 2 cut(s) 25, 575
ArsI GACNNNNNNTTYG 8 cut(s) 257, 289, 303, 335, 396, 428, 530, 562
AspS9I GGNCC 2 cut(s) 442, 637
AsuHPI GGTGA 3 cut(s) 105, 319, 540
AsuII TTCGAA 1 cut(s) 465
AvaI CYCGRG 2 cut(s) 136, 232
AvaII GGWCC 1 cut(s) 637
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 2 cut(s) 239, 372
BbsI GAAGAC 1 cut(s) 409
Bbv12I GWGCWC 3 cut(s) 143, 239, 372
BbvI GCAGC 2 cut(s) 12, 587
BceAI ACGGC 2 cut(s) 146, 330
BfaI CTAG 1 cut(s) 393
BisI GCNGC 2 cut(s) 26, 576
BlsI GCNGC 2 cut(s) 27, 577
Bme18I GGWCC 1 cut(s) 637
BmeT110I CYCGRG 2 cut(s) 136, 232
BmgT120I GGNCC 2 cut(s) 442, 637
BmiI GGNNCC 2 cut(s) 6, 639
BmsI GCATC 2 cut(s) 236, 398
BpiI GAAGAC 1 cut(s) 409
Bpu14I TTCGAA 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 407, 500
BsaWI WCCGGW 1 cut(s) 7
Bse1I ACTGG 2 cut(s) 632, 639
Bse3DI GCAATG 1 cut(s) 554
BseDI CCNNGG 2 cut(s) 407, 500
BseGI GGATG 2 cut(s) 191, 227
BseMI GCAATG 1 cut(s) 554
BseNI ACTGG 2 cut(s) 632, 639
BseRI GAGGAG 3 cut(s) 187, 539, 542
BseXI GCAGC 2 cut(s) 12, 587
Bsh1285I CGRYCG 1 cut(s) 92
BshFI GGCC 3 cut(s) 38, 87, 444
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 92
BsiHKAI GWGCWC 3 cut(s) 143, 239, 372
BsiHKCI CYCGRG 2 cut(s) 136, 232
BsiSI CCGG 1 cut(s) 8
BsnI GGCC 3 cut(s) 38, 87, 444
BsoBI CYCGRG 2 cut(s) 136, 232
Bsp119I TTCGAA 1 cut(s) 465
Bsp1286I GDGCHC 3 cut(s) 143, 239, 372
Bsp143I GATC 3 cut(s) 89, 105, 213
Bsp19I CCATGG 1 cut(s) 407
BspANI GGCC 3 cut(s) 38, 87, 444
BspLI GGNNCC 2 cut(s) 6, 639
BspT104I TTCGAA 1 cut(s) 465
BspT107I GGYRCC 1 cut(s) 4
BsrDI GCAATG 1 cut(s) 554
BsrI ACTGG 2 cut(s) 632, 639
BssECI CCNNGG 2 cut(s) 407, 500
BssMI GATC 3 cut(s) 89, 105, 213
BssNAI GTATAC 1 cut(s) 599
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 407
Bst1107I GTATAC 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 62
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 395
BstBI TTCGAA 1 cut(s) 465
BstC8I GCNNGC 5 cut(s) 368, 421, 457, 534, 573
BstDEI CTNAG 1 cut(s) 616
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 2 cut(s) 191, 227
BstKTI GATC 3 cut(s) 92, 108, 216
BstMBI GATC 3 cut(s) 89, 105, 213
BstMCI CGRYCG 1 cut(s) 92
BstMWI GCNNNNNNNGC 2 cut(s) 93, 395
BstV1I GCAGC 2 cut(s) 12, 587
BstV2I GAAGAC 1 cut(s) 409
BstXI CCANNNNNNTGG 1 cut(s) 270
BstZ17I GTATAC 1 cut(s) 599
BsuRI GGCC 3 cut(s) 38, 87, 444
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 2 cut(s) 191, 227
Cac8I GCNNGC 5 cut(s) 368, 421, 457, 534, 573
Cfr13I GGNCC 2 cut(s) 442, 637
CseI GACGC 1 cut(s) 313
CviAII CATG 2 cut(s) 408, 431
CviJI RGCY 9 cut(s) 25, 38, 87, 133, 237, 370, 444, 455, 578
CviKI_1 RGCY 9 cut(s) 25, 38, 87, 133, 237, 370, 444, 455, 578
DdeI CTNAG 1 cut(s) 616
DpnI GATC 3 cut(s) 91, 107, 215
DpnII GATC 3 cut(s) 89, 105, 213
DraI TTTAAA 1 cut(s) 52
Ecl136II GAGCTC 2 cut(s) 237, 370
Eco130I CCWWGG 1 cut(s) 407
Eco147I AGGCCT 1 cut(s) 38
Eco24I GRGCYC 2 cut(s) 239, 372
Eco47I GGWCC 1 cut(s) 637
Eco53kI GAGCTC 2 cut(s) 237, 370
Eco88I CYCGRG 2 cut(s) 136, 232
EcoICRI GAGCTC 2 cut(s) 237, 370
EcoT14I CCWWGG 1 cut(s) 407
EcoT38I GRGCYC 2 cut(s) 239, 372
ErhI CCWWGG 1 cut(s) 407
FaeI CATG 2 cut(s) 411, 434
FaiI YATR 8 cut(s) 19, 42, 47, 125, 360, 409, 432, 599
FalI AAGNNNNNCTT 4 cut(s) 439, 471, 585, 617
FatI CATG 2 cut(s) 407, 430
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 2 cut(s) 26, 576
FokI GGATG 2 cut(s) 178, 214
FriOI GRGCYC 2 cut(s) 239, 372
Fsp4HI GCNGC 2 cut(s) 26, 576
FspBI CTAG 1 cut(s) 393
GluI GCNGC 2 cut(s) 26, 576
HaeIII GGCC 3 cut(s) 38, 87, 444
HapII CCGG 1 cut(s) 8
HgaI GACGC 1 cut(s) 313
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 2 cut(s) 411, 434
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 8
HphI GGTGA 3 cut(s) 105, 319, 540
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 3 cut(s) 77, 223, 504
Hpy188III TCNNGA 3 cut(s) 136, 163, 581
Hpy8I GTNNAC 1 cut(s) 599
Hpy99I CGWCG 3 cut(s) 132, 323, 524
HpyAV CCTTC 1 cut(s) 159
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 2 cut(s) 71, 318
HpyCH4V TGCA 2 cut(s) 383, 609
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 395
HpyF3I CTNAG 1 cut(s) 616
HpySE526I ACGT 2 cut(s) 71, 318
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 2 cut(s) 411, 434
Kzo9I GATC 3 cut(s) 89, 105, 213
LmnI GCTCC 1 cut(s) 529
LpnPI CCDG 9 cut(s) 21, 129, 176, 221, 384, 553, 566, 613, 620
Lsp1109I GCAGC 2 cut(s) 12, 587
LweI GCATC 2 cut(s) 236, 398
MaeI CTAG 1 cut(s) 393
MaeII ACGT 2 cut(s) 71, 318
MaeIII GTNAC 2 cut(s) 325, 546
MalI GATC 3 cut(s) 91, 107, 215
MboI GATC 3 cut(s) 89, 105, 213
MboII GAAGA 1 cut(s) 414
MhlI GDGCHC 3 cut(s) 143, 239, 372
MluCI AATT 5 cut(s) 101, 120, 182, 298, 621
MnlI CCTC 8 cut(s) 28, 205, 208, 241, 510, 517, 520, 561
MseI TTAA 3 cut(s) 51, 438, 591
MspI CCGG 1 cut(s) 8
MwoI GCNNNNNNNGC 2 cut(s) 93, 395
NcoI CCATGG 1 cut(s) 407
NdeII GATC 3 cut(s) 89, 105, 213
NlaIII CATG 2 cut(s) 411, 434
NlaIV GGNNCC 2 cut(s) 6, 639
NmuCI GTSAC 2 cut(s) 325, 546
NspV TTCGAA 1 cut(s) 465
PaeR7I CTCGAG 2 cut(s) 136, 232
PceI AGGCCT 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 2 cut(s) 27, 577
Ple19I CGATCG 1 cut(s) 92
Psp124BI GAGCTC 2 cut(s) 239, 372
Psp1406I AACGTT 1 cut(s) 71
PspN4I GGNNCC 2 cut(s) 6, 639
PspPI GGNCC 2 cut(s) 442, 637
PspXI VCTCGAGB 1 cut(s) 232
PvuI CGATCG 1 cut(s) 92
SacI GAGCTC 2 cut(s) 239, 372
SaqAI TTAA 3 cut(s) 51, 438, 591
SatI GCNGC 2 cut(s) 26, 576
Sau3AI GATC 3 cut(s) 89, 105, 213
Sau96I GGNCC 2 cut(s) 442, 637
SduI GDGCHC 3 cut(s) 143, 239, 372
SfaNI GCATC 2 cut(s) 236, 398
Sfr274I CTCGAG 2 cut(s) 136, 232
SfuI TTCGAA 1 cut(s) 465
SinI GGWCC 1 cut(s) 637
SlaI CTCGAG 2 cut(s) 136, 232
SmiI ATTTAAAT 1 cut(s) 52
SmlI CTYRAG 2 cut(s) 136, 232
SmoI CTYRAG 2 cut(s) 136, 232
Sse9I AATT 5 cut(s) 101, 120, 182, 298, 621
SseBI AGGCCT 1 cut(s) 38
SspMI CTAG 1 cut(s) 393
SstI GAGCTC 2 cut(s) 239, 372
StuI AGGCCT 1 cut(s) 38
StyI CCWWGG 1 cut(s) 407
SwaI ATTTAAAT 1 cut(s) 52
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 2 cut(s) 74, 321
TaqI TCGA 5 cut(s) 92, 137, 212, 233, 465
TasI AATT 5 cut(s) 101, 120, 182, 298, 621
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 3 cut(s) 51, 438, 591
Tru9I TTAA 3 cut(s) 51, 438, 591
TseFI GTSAC 2 cut(s) 325, 546
TseI GCWGC 2 cut(s) 25, 575
Tsp45I GTSAC 2 cut(s) 325, 546
TspGWI ACGGA 2 cut(s) 142, 231
VpaK11BI GGWCC 1 cut(s) 637
XhoI CTCGAG 2 cut(s) 136, 232
XmiI GTMKAC 1 cut(s) 598
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.