pycom03g01930

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011421
Physical Location & Seq
Reverse (-)
1451060 .. 1451392
333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g01930.1

Sequence Viewer

Length: 333 bp
ATGCATGCAGGTGTGGATAGCTTGGAAATAAACATGGATACACAAAAGGTGACGGTGACGGGGTATGTAGATCAAAGAAAGGTGTTAAAGGTTGTGAGAAGAACAGGGAGGAGGGCTGAATTTTGGCCCTTCCCATACGACAGCGAATACTATCCGTACGCATCTCAATACTTTGACGAGTCTACGTATTCGTCTTCCTACAACTACTACATGCATGGCTATAACGAAAGCGTGCACGGATACTTCCCCGACCAGTTATACTCAACCGTTGCTGATAATACTGTACATCTCTTTAGTGACGACAATGTTCATGCCTACTGCACTCTTATGTGA

Protein Analysis

111

Amino Acids

12.95

Weight (kDa)

5.12

Isoelectric Point (pI)

49.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 4 - 37 6.2e-06 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56891 AT3G56891
fragaria_vesca FvH4_2g26780
malus_domestica MD03G1023000.v1.1
prunus_persica Prupe.7G025000_v2.0.a1
pyrus_communis pycom03g01930
rosa_chinensis RchiOBHm_Chr3g0495281
rosa_laevigata RLG00000022631
rosa_multiflora Rmu_co8042714.1_g000001 Rmu_sc0002433.1_g000005 Rmu_sc0002433.1_g000007
rosa_roxburghii Rroxscaffold_6G00390640
rosa_rugosa Rorug03G0272000 Rorug03G0272100 Rorug03G0272200
rosa_samantha Rh3AG320400 Rh3BG355200 Rh3CG352200 Rh3DG354300
rosa_wichuraiana Rw3G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 182
AcsI RAATTY 1 cut(s) 119
AfaI GTAC 2 cut(s) 158, 285
AluBI AGCT 1 cut(s) 21
AluI AGCT 1 cut(s) 21
Alw21I GWGCWC 1 cut(s) 237
Alw44I GTGCAC 1 cut(s) 233
AoxI GGCC 1 cut(s) 125
ApaLI GTGCAC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 126
AsuHPI GGTGA 2 cut(s) 61, 67
BaeGI GKGCMC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 237
BciVI GTATCC 2 cut(s) 31, 233
BfuI GTATCC 2 cut(s) 31, 233
BmgT120I GGNCC 1 cut(s) 126
BmsI GCATC 1 cut(s) 170
BpiI GAAGAC 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 253
BseNI ACTGG 1 cut(s) 253
BseRI GAGGAG 1 cut(s) 124
BseSI GKGCMC 1 cut(s) 237
BsgI GTGCAG 1 cut(s) 304
BshFI GGCC 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 237
BsiWI CGTACG 1 cut(s) 156
BsnI GGCC 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 237
Bsp1407I TGTACA 1 cut(s) 283
Bsp143I GATC 1 cut(s) 70
BspANI GGCC 1 cut(s) 127
BsrGI TGTACA 1 cut(s) 283
BsrI ACTGG 1 cut(s) 253
BssMI GATC 1 cut(s) 70
Bst4CI ACNGT 3 cut(s) 55, 268, 283
BstAUI TGTACA 1 cut(s) 283
BstBAI YACGTR 1 cut(s) 186
BstC8I GCNNGC 2 cut(s) 6, 233
BstKTI GATC 1 cut(s) 73
BstMBI GATC 1 cut(s) 70
BstNSI RCATGY 2 cut(s) 8, 214
BstSLI GKGCMC 1 cut(s) 237
BstSNI TACGTA 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 186
BsuI GTATCC 2 cut(s) 31, 233
BsuRI GGCC 1 cut(s) 127
Cac8I GCNNGC 2 cut(s) 6, 233
Cfr13I GGNCC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 157, 284
CviAII CATG 5 cut(s) 5, 34, 211, 215, 311
CviJI RGCY 4 cut(s) 21, 116, 127, 219
CviKI_1 RGCY 4 cut(s) 21, 116, 127, 219
CviQI GTAC 2 cut(s) 157, 284
DpnI GATC 1 cut(s) 72
DpnII GATC 1 cut(s) 70
Eco105I TACGTA 1 cut(s) 186
EcoT22I ATGCAT 2 cut(s) 6, 216
FaeI CATG 5 cut(s) 8, 37, 214, 218, 314
FatI CATG 5 cut(s) 4, 33, 210, 214, 310
FblI GTMKAC 1 cut(s) 182
HaeIII GGCC 1 cut(s) 127
Hin1II CATG 5 cut(s) 8, 37, 214, 218, 314
HinfI GANTC 1 cut(s) 179
HphI GGTGA 2 cut(s) 61, 67
Hpy166II GTNNAC 2 cut(s) 183, 235
Hpy8I GTNNAC 2 cut(s) 183, 235
HpyAV CCTTC 1 cut(s) 139
HpyCH4III ACNGT 3 cut(s) 55, 268, 283
HpyCH4IV ACGT 1 cut(s) 185
HpyCH4V TGCA 5 cut(s) 4, 8, 214, 235, 321
HpySE526I ACGT 1 cut(s) 185
Hsp92II CATG 5 cut(s) 8, 37, 214, 218, 314
Kzo9I GATC 1 cut(s) 70
LpnPI CCDG 2 cut(s) 90, 266
LweI GCATC 1 cut(s) 170
MaeII ACGT 1 cut(s) 185
MaeIII GTNAC 3 cut(s) 49, 55, 296
MalI GATC 1 cut(s) 72
MboI GATC 1 cut(s) 70
MboII GAAGA 2 cut(s) 111, 186
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 1 cut(s) 119
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 2 cut(s) 102, 105
Mph1103I ATGCAT 2 cut(s) 6, 216
MseI TTAA 1 cut(s) 86
MslI CAYNNNNRTG 2 cut(s) 9, 326
NdeII GATC 1 cut(s) 70
NlaIII CATG 5 cut(s) 8, 37, 214, 218, 314
NmuCI GTSAC 3 cut(s) 49, 55, 296
NsiI ATGCAT 2 cut(s) 6, 216
NspI RCATGY 2 cut(s) 8, 214
PaeI GCATGC 1 cut(s) 8
Pfl23II CGTACG 1 cut(s) 156
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 186
PspLI CGTACG 1 cut(s) 156
PspPI GGNCC 1 cut(s) 126
RsaI GTAC 2 cut(s) 158, 285
RsaNI GTAC 2 cut(s) 157, 284
RseI CAYNNNNRTG 2 cut(s) 9, 326
SaqAI TTAA 1 cut(s) 86
Sau3AI GATC 1 cut(s) 70
Sau96I GGNCC 1 cut(s) 126
SchI GAGTC 1 cut(s) 188
SduI GDGCHC 1 cut(s) 237
SetI ASST 6 cut(s) 13, 23, 51, 84, 93, 188
SfaNI GCATC 1 cut(s) 170
SmiMI CAYNNNNRTG 2 cut(s) 9, 326
SnaBI TACGTA 1 cut(s) 186
SphI GCATGC 1 cut(s) 8
Sse9I AATT 1 cut(s) 119
TaaI ACNGT 3 cut(s) 55, 268, 283
TaiI ACGT 1 cut(s) 188
TasI AATT 1 cut(s) 119
TatI WGTACW 1 cut(s) 283
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 3 cut(s) 49, 55, 296
Tsp45I GTSAC 3 cut(s) 49, 55, 296
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 2 cut(s) 144, 252
VneI GTGCAC 1 cut(s) 233
XapI RAATTY 1 cut(s) 119
XceI RCATGY 2 cut(s) 8, 214
XmiI GTMKAC 1 cut(s) 182
Zsp2I ATGCAT 2 cut(s) 6, 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.