Rw3G027970

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011421
Physical Location & Seq
Forward (+)
40730866 .. 40731556
691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027970.1

Sequence Viewer

Length: 378 bp
ATGGATTGTGAAGGATGTGAAAAGAGGATTCGCCGAGCAATTTCAAAAATAGATGGTGTGGATAGCTTGGAAATAGATATGGATAAGCAAAAGGTGACAGTGACCGGATATGTGGATCAAAGGAAAGTGCTGAAGGTGGTGAGACGAACGGGAAGGAAAGCCGAGTTTTGGCCATTTCCATATGACTCCGAATACTATCCCTACGCATCTCAATACTTCGACGAGTCAACCTACTCGTCATCGTATAACTACTACATGCACGGCTATAATGAAGCCATTCATGGATATTTCCCTGACCCTCTGTATTCGACTGTATCTGATCAAACTGTTCATCTTTTCAGTGATGACAATGTTCATGCATATTGCACCGTTATGTAA

Protein Analysis

125

Amino Acids

14.7

Weight (kDa)

5.26

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 1 - 53 2.2e-16 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56891 AT3G56891
fragaria_vesca FvH4_2g26780
malus_domestica MD03G1023000.v1.1
prunus_persica Prupe.7G025000_v2.0.a1
pyrus_communis pycom03g01930
rosa_chinensis RchiOBHm_Chr3g0495281
rosa_laevigata RLG00000022631
rosa_multiflora Rmu_co8042714.1_g000001 Rmu_sc0002433.1_g000005 Rmu_sc0002433.1_g000007
rosa_roxburghii Rroxscaffold_6G00390640
rosa_rugosa Rorug03G0272000 Rorug03G0272100 Rorug03G0272200
rosa_samantha Rh3AG320400 Rh3BG355200 Rh3CG352200 Rh3DG354300
rosa_wichuraiana Rw3G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 123
AcoI YGGCCR 1 cut(s) 170
AcuI CTGAAG 1 cut(s) 152
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 1 cut(s) 45
AluBI AGCT 1 cut(s) 66
AluI AGCT 1 cut(s) 66
Alw26I GTCTC 1 cut(s) 136
AlwI GGATC 1 cut(s) 123
AoxI GGCC 1 cut(s) 170
Asp700I GAANNNNTTC 1 cut(s) 276
AsuHPI GGTGA 2 cut(s) 106, 151
BalI TGGCCA 1 cut(s) 172
BccI CCATC 1 cut(s) 47
BceAI ACGGC 1 cut(s) 277
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 136
BmsI GCATC 1 cut(s) 215
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 20
BseLI CCNNNNNNNGG 1 cut(s) 168
BshFI GGCC 1 cut(s) 172
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 136
BsmBI CGTCTC 1 cut(s) 136
BsnI GGCC 1 cut(s) 172
Bsp143I GATC 2 cut(s) 115, 319
BspANI GGCC 1 cut(s) 172
BspPI GGATC 1 cut(s) 123
BssMI GATC 2 cut(s) 115, 319
Bst4CI ACNGT 4 cut(s) 100, 313, 328, 370
BstF5I GGATG 1 cut(s) 20
BstKTI GATC 2 cut(s) 118, 322
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 2 cut(s) 115, 319
BstNSI RCATGY 1 cut(s) 259
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 105, 346
CviAII CATG 3 cut(s) 256, 281, 356
CviJI RGCY 5 cut(s) 66, 161, 172, 264, 275
CviKI_1 RGCY 5 cut(s) 66, 161, 172, 264, 275
DpnI GATC 2 cut(s) 117, 321
DpnII GATC 2 cut(s) 115, 319
EaeI YGGCCR 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 152
EcoT22I ATGCAT 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 3 cut(s) 259, 284, 359
FatI CATG 3 cut(s) 255, 280, 355
FauNDI CATATG 1 cut(s) 181
FbaI TGATCA 1 cut(s) 319
FokI GGATG 1 cut(s) 27
HaeIII GGCC 1 cut(s) 172
HapII CCGG 1 cut(s) 105
Hin1II CATG 3 cut(s) 259, 284, 359
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 3 cut(s) 28, 185, 224
HpaII CCGG 1 cut(s) 105
HphI GGTGA 2 cut(s) 106, 151
Hpy166II GTNNAC 1 cut(s) 228
Hpy188I TCNGA 2 cut(s) 190, 319
Hpy8I GTNNAC 1 cut(s) 228
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 3 cut(s) 5, 127, 147
HpyCH4III ACNGT 4 cut(s) 100, 313, 328, 370
HpyCH4V TGCA 3 cut(s) 259, 359, 366
Hsp92II CATG 3 cut(s) 259, 284, 359
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 2 cut(s) 115, 319
LpnPI CCDG 2 cut(s) 118, 306
LweI GCATC 1 cut(s) 215
MaeIII GTNAC 2 cut(s) 94, 100
MalI GATC 2 cut(s) 117, 321
MboI GATC 2 cut(s) 115, 319
MlsI TGGCCA 1 cut(s) 172
MluCI AATT 1 cut(s) 39
MluNI TGGCCA 1 cut(s) 172
MlyI GAGTC 2 cut(s) 179, 233
MnlI CCTC 2 cut(s) 18, 309
Mox20I TGGCCA 1 cut(s) 172
Mph1103I ATGCAT 1 cut(s) 361
MroXI GAANNNNTTC 1 cut(s) 276
MscI TGGCCA 1 cut(s) 172
MslI CAYNNNNRTG 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 172
MspI CCGG 1 cut(s) 105
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 115, 319
NlaIII CATG 3 cut(s) 259, 284, 359
NmeAIII GCCGAG 2 cut(s) 59, 187
NmuCI GTSAC 2 cut(s) 94, 100
NsiI ATGCAT 1 cut(s) 361
NspI RCATGY 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 276
PfeI GAWTC 1 cut(s) 28
PleI GAGTC 2 cut(s) 179, 232
PpsI GAGTC 2 cut(s) 179, 232
RseI CAYNNNNRTG 1 cut(s) 371
Sau3AI GATC 2 cut(s) 115, 319
SchI GAGTC 2 cut(s) 179, 233
SetI ASST 4 cut(s) 68, 96, 138, 233
SfaNI GCATC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 371
Sse9I AATT 1 cut(s) 39
TaaI ACNGT 4 cut(s) 100, 313, 328, 370
TaqI TCGA 2 cut(s) 219, 308
TasI AATT 1 cut(s) 39
TfiI GAWTC 1 cut(s) 28
TscAI CASTG 2 cut(s) 105, 346
TseFI GTSAC 2 cut(s) 94, 100
Tsp45I GTSAC 2 cut(s) 94, 100
TspDTI ATGAA 4 cut(s) 269, 285, 320, 344
TspRI CASTG 2 cut(s) 105, 346
XceI RCATGY 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 276
Zsp2I ATGCAT 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.