Rroxscaffold_6G00390640

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011421
Physical Location & Seq
Reverse (-)
6090684 .. 6092960
2277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00390640.1

Sequence Viewer

Length: 507 bp
ATGAGTTCCCCTTCTCCTCTTGGTTCTCCGGTATATGTTGAATTGCTTGATACAGAGTCCAATCACATAGAGGATTCTCAAGGGGATTCGACCGATACTTCTAAAGAGGACCATGTTAGTTTTTCAAAAGAGGCGTCTGATCATGTGCTCGAGGAGCCTCTCAAGGATGTAATTAATCAAGATGGTGTGGATAGCTTGGAAATAGATATGGATAAGCAAAAGGTGACAGTGACCGGATATGTGGATCAAAGGAAGGTGTTGAAGGTGGTGAGACGAACAGGAAGGAAAGCCGAGTTTTGGCCATTTCCATATGACTCTGAATACTATCCCTACGCATCTCAATACTTCGACGAGTCAACCTACTCGTCGTCGTATAACTACTACATGCACGGCTATAATGAAGCCATTCGTGGATATTTCCCTGACCCTCTGTATTCGACTGTTTCTGATCAAACTGTTCATCTTTTCAGTGATGACAATGTTCATGCATATTGTACCCTTATGTAA

Protein Analysis

168

Amino Acids

19.29

Weight (kDa)

4.45

Isoelectric Point (pI)

59.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 59 - 96 1.7e-06 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56891 AT3G56891
fragaria_vesca FvH4_2g26780
malus_domestica MD03G1023000.v1.1
prunus_persica Prupe.7G025000_v2.0.a1
pyrus_communis pycom03g01930
rosa_chinensis RchiOBHm_Chr3g0495281
rosa_laevigata RLG00000022631
rosa_multiflora Rmu_co8042714.1_g000001 Rmu_sc0002433.1_g000005 Rmu_sc0002433.1_g000007
rosa_roxburghii Rroxscaffold_6G00390640
rosa_rugosa Rorug03G0272000 Rorug03G0272100 Rorug03G0272200
rosa_samantha Rh3AG320400 Rh3BG355200 Rh3CG352200 Rh3DG354300
rosa_wichuraiana Rw3G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 252
AcoI YGGCCR 1 cut(s) 299
AcyI GRCGYC 1 cut(s) 134
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 1 cut(s) 297
AgsI TTSAA 3 cut(s) 41, 126, 262
AluBI AGCT 1 cut(s) 195
AluI AGCT 1 cut(s) 195
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 265
AlwI GGATC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 1 cut(s) 299
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
AseI ATTAAT 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 405
AspS9I GGNCC 1 cut(s) 109
AsuHPI GGTGA 2 cut(s) 235, 280
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 301
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 1 cut(s) 176
BceAI ACGGC 1 cut(s) 406
BclI TGATCA 2 cut(s) 139, 448
BcoDI GTCTC 1 cut(s) 265
Bme18I GGWCC 1 cut(s) 109
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 109
BmiI GGNNCC 1 cut(s) 156
BmsI GCATC 1 cut(s) 344
BpuEI CTTGAG 2 cut(s) 63, 146
BsaHI GRCGYC 1 cut(s) 134
BsaWI WCCGGW 2 cut(s) 28, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 297
BseRI GAGGAG 2 cut(s) 6, 167
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 2 cut(s) 29, 234
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 1 cut(s) 265
BsmBI CGTCTC 1 cut(s) 265
BsnI GGCC 1 cut(s) 301
BsoBI CYCGRG 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 3 cut(s) 139, 244, 448
BspANI GGCC 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 156
BspPI GGATC 1 cut(s) 252
BssMI GATC 3 cut(s) 139, 244, 448
BssNI GRCGYC 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 229, 442, 457
BstACI GRCGYC 1 cut(s) 134
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 3 cut(s) 142, 247, 451
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 3 cut(s) 139, 244, 448
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNSI RCATGY 1 cut(s) 388
BsuRI GGCC 1 cut(s) 301
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 2 cut(s) 234, 475
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 1 cut(s) 123
Csp6I GTAC 1 cut(s) 495
CviAII CATG 4 cut(s) 113, 143, 385, 485
CviJI RGCY 6 cut(s) 157, 195, 290, 301, 393, 404
CviKI_1 RGCY 6 cut(s) 157, 195, 290, 301, 393, 404
CviQI GTAC 1 cut(s) 495
DpnI GATC 3 cut(s) 141, 246, 450
DpnII GATC 3 cut(s) 139, 244, 448
EaeI YGGCCR 1 cut(s) 299
Eco47I GGWCC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 149
EcoT22I ATGCAT 1 cut(s) 490
Esp3I CGTCTC 1 cut(s) 265
FaeI CATG 4 cut(s) 116, 146, 388, 488
FatI CATG 4 cut(s) 112, 142, 384, 484
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 2 cut(s) 139, 448
FokI GGATG 1 cut(s) 179
HaeIII GGCC 1 cut(s) 301
HapII CCGG 2 cut(s) 29, 234
HgaI GACGC 1 cut(s) 123
Hin1I GRCGYC 1 cut(s) 134
Hin1II CATG 4 cut(s) 116, 146, 388, 488
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 5 cut(s) 56, 74, 86, 314, 353
HpaII CCGG 2 cut(s) 29, 234
HphI GGTGA 2 cut(s) 235, 280
Hpy166II GTNNAC 1 cut(s) 357
Hpy188I TCNGA 3 cut(s) 139, 319, 448
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 1 cut(s) 357
Hpy99I CGWCG 3 cut(s) 353, 370, 373
HpyAV CCTTC 4 cut(s) 21, 247, 256, 276
HpyCH4III ACNGT 3 cut(s) 229, 442, 457
HpyCH4V TGCA 2 cut(s) 388, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
Hsp92I GRCGYC 1 cut(s) 134
Hsp92II CATG 4 cut(s) 116, 146, 388, 488
Ksp22I TGATCA 2 cut(s) 139, 448
Kzo9I GATC 3 cut(s) 139, 244, 448
LmnI GCTCC 1 cut(s) 154
LpnPI CCDG 4 cut(s) 42, 247, 264, 435
LweI GCATC 1 cut(s) 344
MaeIII GTNAC 2 cut(s) 223, 229
MalI GATC 3 cut(s) 141, 246, 450
MboI GATC 3 cut(s) 139, 244, 448
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 301
MluCI AATT 2 cut(s) 41, 171
MluNI TGGCCA 1 cut(s) 301
MlyI GAGTC 3 cut(s) 65, 308, 362
MnlI CCTC 7 cut(s) 27, 64, 100, 124, 145, 168, 438
Mox20I TGGCCA 1 cut(s) 301
Mph1103I ATGCAT 1 cut(s) 490
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 1 cut(s) 301
MseI TTAA 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 301
MspI CCGG 2 cut(s) 29, 234
MwoI GCNNNNNNNGC 1 cut(s) 154
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 139, 244, 448
NlaIII CATG 4 cut(s) 116, 146, 388, 488
NlaIV GGNNCC 1 cut(s) 156
NmeAIII GCCGAG 1 cut(s) 316
NmuCI GTSAC 2 cut(s) 223, 229
NsiI ATGCAT 1 cut(s) 490
NspI RCATGY 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 2 cut(s) 74, 86
PleI GAGTC 3 cut(s) 64, 308, 361
PpsI GAGTC 3 cut(s) 64, 308, 361
PshBI ATTAAT 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 156
PspPI GGNCC 1 cut(s) 109
PspXI VCTCGAGB 1 cut(s) 149
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
SaqAI TTAA 1 cut(s) 174
Sau3AI GATC 3 cut(s) 139, 244, 448
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 3 cut(s) 65, 308, 362
SduI GDGCHC 1 cut(s) 150
SetI ASST 5 cut(s) 197, 225, 258, 267, 362
SfaNI GCATC 1 cut(s) 344
Sfr274I CTCGAG 1 cut(s) 149
SinI GGWCC 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 3 cut(s) 78, 149, 161
SmoI CTYRAG 3 cut(s) 78, 149, 161
Sse9I AATT 2 cut(s) 41, 171
TaaI ACNGT 3 cut(s) 229, 442, 457
TaqI TCGA 4 cut(s) 89, 150, 348, 437
TaqII GACCGA 1 cut(s) 107
TasI AATT 2 cut(s) 41, 171
TfiI GAWTC 2 cut(s) 74, 86
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 2 cut(s) 234, 475
TseFI GTSAC 2 cut(s) 223, 229
Tsp45I GTSAC 2 cut(s) 223, 229
TspDTI ATGAA 3 cut(s) 414, 449, 473
TspRI CASTG 2 cut(s) 234, 475
VpaK11BI GGWCC 1 cut(s) 109
VspI ATTAAT 1 cut(s) 174
XceI RCATGY 1 cut(s) 388
XhoI CTCGAG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 405
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.