Rh3DG354300

Heavy metal-associated isoprenylated plant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0011421
Physical Location & Seq
Forward (+)
42871594 .. 42872220
627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG354300.1

Sequence Viewer

Length: 450 bp
ATGTTTGGTTGGCGACATGGGAAAACATCACAAATATCTAATGCCATCTCAATTGTGGAACTCTTGGTGCATATGGATTGTGAAGGATGTGAAAAGAGGATTCGCCGAGCAATTTCAAAAATAGATGGTGTGGATAGCTTGGAAATAGATATGGATAAGCAAAAGGTGACAGTGACCGGATATGTGGATCAAAGGAAAGTGTTGAAGGTGGTGAGACGAACGGGAAGGAAAGCCGAGTTTTGGCCATTTCCATATGACTCCGAATACTATCCCTACGCATCTCAATACTTCGACGAGTCAACCTACTCGTCATCGTATAACTACTACATGCACGGCTATAATGAAGCCATTCATGGATATTTCCCTGACCCTCTGTATTCGACTGTATCTGATCAAACTGTTCATCTTTTCAGTGATGACAATGTTCATGCATATTGCACCGTTATGTAA

Protein Analysis

149

Amino Acids

17.4

Weight (kDa)

5.82

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 23 - 77 8e-17 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011421)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56891 AT3G56891
fragaria_vesca FvH4_2g26780
malus_domestica MD03G1023000.v1.1
prunus_persica Prupe.7G025000_v2.0.a1
pyrus_communis pycom03g01930
rosa_chinensis RchiOBHm_Chr3g0495281
rosa_laevigata RLG00000022631
rosa_multiflora Rmu_co8042714.1_g000001 Rmu_sc0002433.1_g000005 Rmu_sc0002433.1_g000007
rosa_roxburghii Rroxscaffold_6G00390640
rosa_rugosa Rorug03G0272000 Rorug03G0272100 Rorug03G0272200
rosa_samantha Rh3AG320400 Rh3BG355200 Rh3CG352200 Rh3DG354300
rosa_wichuraiana Rw3G027970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 195
AcoI YGGCCR 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 240
AgsI TTSAA 2 cut(s) 117, 205
AluBI AGCT 1 cut(s) 138
AluI AGCT 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 208
AlwI GGATC 1 cut(s) 195
AoxI GGCC 1 cut(s) 242
Asp700I GAANNNNTTC 1 cut(s) 348
AsuHPI GGTGA 2 cut(s) 178, 223
BalI TGGCCA 1 cut(s) 244
BccI CCATC 2 cut(s) 53, 119
BceAI ACGGC 1 cut(s) 349
BclI TGATCA 1 cut(s) 391
BcoDI GTCTC 1 cut(s) 208
BmsI GCATC 1 cut(s) 287
BsaWI WCCGGW 1 cut(s) 176
Bsc4I CCNNNNNNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 92
BseLI CCNNNNNNNGG 1 cut(s) 240
BshFI GGCC 1 cut(s) 244
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 1 cut(s) 240
BsmAI GTCTC 1 cut(s) 208
BsmBI CGTCTC 1 cut(s) 208
BsnI GGCC 1 cut(s) 244
Bsp143I GATC 2 cut(s) 187, 391
BspANI GGCC 1 cut(s) 244
BspPI GGATC 1 cut(s) 195
BssMI GATC 2 cut(s) 187, 391
Bst4CI ACNGT 4 cut(s) 172, 385, 400, 442
BstF5I GGATG 1 cut(s) 92
BstKTI GATC 2 cut(s) 190, 394
BstMAI GTCTC 1 cut(s) 208
BstMBI GATC 2 cut(s) 187, 391
BstNSI RCATGY 1 cut(s) 331
BsuRI GGCC 1 cut(s) 244
BtsCI GGATG 1 cut(s) 92
BtsIMutI CAGTG 2 cut(s) 177, 418
CviAII CATG 4 cut(s) 17, 328, 353, 428
CviJI RGCY 5 cut(s) 138, 233, 244, 336, 347
CviKI_1 RGCY 5 cut(s) 138, 233, 244, 336, 347
DpnI GATC 2 cut(s) 189, 393
DpnII GATC 2 cut(s) 187, 391
EaeI YGGCCR 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 433
Esp3I CGTCTC 1 cut(s) 208
FaeI CATG 4 cut(s) 20, 331, 356, 431
FatI CATG 4 cut(s) 16, 327, 352, 427
FauNDI CATATG 2 cut(s) 72, 253
FbaI TGATCA 1 cut(s) 391
FokI GGATG 1 cut(s) 99
HaeIII GGCC 1 cut(s) 244
HapII CCGG 1 cut(s) 177
Hin1II CATG 4 cut(s) 20, 331, 356, 431
HincII GTYRAC 1 cut(s) 300
HindII GTYRAC 1 cut(s) 300
HinfI GANTC 3 cut(s) 100, 257, 296
HpaII CCGG 1 cut(s) 177
HphI GGTGA 2 cut(s) 178, 223
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 2 cut(s) 262, 391
Hpy8I GTNNAC 1 cut(s) 300
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 3 cut(s) 77, 199, 219
HpyCH4III ACNGT 4 cut(s) 172, 385, 400, 442
HpyCH4V TGCA 4 cut(s) 70, 331, 431, 438
Hsp92II CATG 4 cut(s) 20, 331, 356, 431
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 2 cut(s) 187, 391
LpnPI CCDG 2 cut(s) 190, 378
LweI GCATC 1 cut(s) 287
MaeIII GTNAC 2 cut(s) 166, 172
MalI GATC 2 cut(s) 189, 393
MboI GATC 2 cut(s) 187, 391
MfeI CAATTG 1 cut(s) 51
MlsI TGGCCA 1 cut(s) 244
MluCI AATT 2 cut(s) 51, 111
MluNI TGGCCA 1 cut(s) 244
MlyI GAGTC 2 cut(s) 251, 305
MnlI CCTC 2 cut(s) 90, 381
Mox20I TGGCCA 1 cut(s) 244
Mph1103I ATGCAT 1 cut(s) 433
MroXI GAANNNNTTC 1 cut(s) 348
MscI TGGCCA 1 cut(s) 244
MslI CAYNNNNRTG 1 cut(s) 443
Msp20I TGGCCA 1 cut(s) 244
MspI CCGG 1 cut(s) 177
MunI CAATTG 1 cut(s) 51
NdeI CATATG 2 cut(s) 72, 253
NdeII GATC 2 cut(s) 187, 391
NlaIII CATG 4 cut(s) 20, 331, 356, 431
NmeAIII GCCGAG 2 cut(s) 131, 259
NmuCI GTSAC 2 cut(s) 166, 172
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 1 cut(s) 331
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 1 cut(s) 100
PleI GAGTC 2 cut(s) 251, 304
PpsI GAGTC 2 cut(s) 251, 304
RseI CAYNNNNRTG 1 cut(s) 443
Sau3AI GATC 2 cut(s) 187, 391
SchI GAGTC 2 cut(s) 251, 305
SetI ASST 4 cut(s) 140, 168, 210, 305
SfaNI GCATC 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 443
Sse9I AATT 2 cut(s) 51, 111
TaaI ACNGT 4 cut(s) 172, 385, 400, 442
TaqI TCGA 2 cut(s) 291, 380
TasI AATT 2 cut(s) 51, 111
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 2 cut(s) 177, 418
TseFI GTSAC 2 cut(s) 166, 172
Tsp45I GTSAC 2 cut(s) 166, 172
TspDTI ATGAA 4 cut(s) 341, 357, 392, 416
TspRI CASTG 2 cut(s) 177, 418
XceI RCATGY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.