pycom03g02820

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007812
Physical Location & Seq
Forward (+)
2205462 .. 2208092
2631 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 957 bp
ATGTCCAAAATGAAACTCTACAACTATCACCAAAAACACCTCCCTAGCCCAAGGAGAAGAAAAGCCCAAATAATAACCACCCAATTCCGGGAAACCACCACGCCGCCATTTTTCTGTCAATCAAAAACAAAAACAAAAAAAAACCGTTTCGACAAAGAAACTCGCAAACCAAAAAGAAACGTGCTTTCCTTTCCATTCTCCAATTTTAAATTCCCCGAAAGCACAAAGCCAGCGACTTTCCCGATTCCACGGCGAAGCTTCTTCCCGATGCAGAAGATGGCGCAGGAATGGTTTTCGCAGACCGGCAGCGGCGTCGACGACTCGCAGAAGCCGTCGTCGTCTTCTTTGCTGGCCGATTGGAACTCCTACGCCGCTTCACAATCCACTGACGAGAGCTCCTCTTTGGGACTCGGATTCGATCTCGAATCTGCGGTGCGCTCTGCTAACGACACCGTTTCCGGCACTTTCAGTGTGGTTTCAAAAGGAGTGAGAGATATACCTGGGAGCCTCCAGTCTGCTACTAGTAATGTCCCTTCAGGAAAAGCTCTCATGTATTTTGGCTTGTTTCTAGCCACTGGGGTGTTCTTTGTTTTTATCGCATTTACCATGTTCCTTCCTGTCATGGTGTTGATGCCTCAGAAGTTTGCTATCTGCTTTACACTTGGGTGTGCCTTTATTATCGCATCATTCTTTGCACTCAAGGGTCCAAAGAATCAGCTTGCTCACATGTCTTCAAAAGAGAGACTTCCGTTTACCGTTGGATTTTTAGGCAGTATGGTGGGTACCATATATGTATCCATGGTACTCCACAGTTACATCCTCTCTGTTTTCTTCTCTGTGTTACAGGTTCTGGCACTTGCATACTATGCTGTTTCATACTTTCCTGGTGGATCTGCGGGTTTGAAGTTCCTATCTTCTGCCCTTACATCTTCAGTAATGCGATGTTTTGGGAGGTGA

Protein Analysis

319

Amino Acids

35.29

Weight (kDa)

10.04

Isoelectric Point (pI)

51.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 183 - 295 3e-29 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 782
AccI GTMKAC 1 cut(s) 315
AciI CCGC 5 cut(s) 104, 309, 372, 431, 896
AclWI GGATC 1 cut(s) 898
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 1 cut(s) 209
AcuI CTGAAG 2 cut(s) 519, 915
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 2 cut(s) 784, 804
AfiI CCNNNNNNNGG 2 cut(s) 87, 88
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 3 cut(s) 480, 735, 904
AhlI ACTAGT 1 cut(s) 521
AjnI CCWGG 2 cut(s) 499, 883
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AleI CACNNNNGTG 2 cut(s) 578, 664
AluBI AGCT 4 cut(s) 258, 396, 545, 718
AluI AGCT 4 cut(s) 258, 396, 545, 718
Alw21I GWGCWC 1 cut(s) 398
Alw26I GTCTC 1 cut(s) 736
AlwI GGATC 1 cut(s) 898
AlwNI CAGNNNCTG 1 cut(s) 850
AoxI GGCC 1 cut(s) 351
ApeKI GCWGC 1 cut(s) 306
ApoI RAATTY 1 cut(s) 209
Asp718I GGTACC 1 cut(s) 782
AspLEI GCGC 2 cut(s) 283, 438
AspS9I GGNCC 1 cut(s) 704
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 20
AvaII GGWCC 1 cut(s) 704
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 398
BarI GAAGNNNNNNTAC 2 cut(s) 517, 549
BbsI GAAGAC 2 cut(s) 333, 723
Bbv12I GWGCWC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 318
BccI CCATC 1 cut(s) 271
BceAI ACGGC 2 cut(s) 266, 316
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 2 cut(s) 501, 885
BciVI GTATCC 1 cut(s) 805
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 736
BcuI ACTAGT 1 cut(s) 521
BfaI CTAG 3 cut(s) 45, 522, 569
BfuI GTATCC 1 cut(s) 805
BisI GCNGC 4 cut(s) 104, 307, 310, 372
BlsI GCNGC 4 cut(s) 105, 308, 311, 373
Bme1390I CCNGG 3 cut(s) 89, 501, 885
Bme18I GGWCC 1 cut(s) 704
BmgT120I GGNCC 1 cut(s) 704
BmiI GGNNCC 3 cut(s) 506, 705, 784
BmrFI CCNGG 3 cut(s) 89, 501, 885
BmrI ACTGGG 1 cut(s) 585
BmsI GCATC 3 cut(s) 258, 621, 692
BmuI ACTGGG 1 cut(s) 585
BpiI GAAGAC 2 cut(s) 333, 723
BplI GAGNNNNNCTC 2 cut(s) 383, 415
BpmI CTGGAG 1 cut(s) 494
BpuEI CTTGAG 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 4 cut(s) 50, 248, 500, 798
BsaXI ACNNNNNCTCC 2 cut(s) 380, 410
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 88
Bse118I RCCGGY 1 cut(s) 302
Bse1I ACTGG 2 cut(s) 511, 580
BseBI CCWGG 2 cut(s) 501, 885
BseDI CCNNGG 4 cut(s) 50, 248, 500, 798
BseGI GGATG 1 cut(s) 816
BseLI CCNNNNNNNGG 2 cut(s) 87, 88
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 2 cut(s) 511, 580
BseRI GAGGAG 1 cut(s) 388
BseXI GCAGC 1 cut(s) 318
BshFI GGCC 1 cut(s) 353
BshNI GGYRCC 1 cut(s) 782
BsiHKAI GWGCWC 1 cut(s) 398
BsiSI CCGG 3 cut(s) 88, 303, 459
BslFI GGGAC 2 cut(s) 420, 515
BslI CCNNNNNNNGG 2 cut(s) 87, 88
BsmAI GTCTC 1 cut(s) 736
BsmFI GGGAC 2 cut(s) 420, 515
BsnI GGCC 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 398
Bsp143I GATC 2 cut(s) 418, 890
Bsp19I CCATGG 1 cut(s) 798
BspACI CCGC 5 cut(s) 104, 309, 372, 431, 896
BspANI GGCC 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 649
BspLI GGNNCC 3 cut(s) 506, 705, 784
BspPI GGATC 1 cut(s) 898
BspT107I GGYRCC 1 cut(s) 782
BsrFI RCCGGY 1 cut(s) 302
BsrI ACTGG 2 cut(s) 511, 580
BssAI RCCGGY 1 cut(s) 302
BssECI CCNNGG 4 cut(s) 50, 248, 500, 798
BssMI GATC 2 cut(s) 418, 890
BssNI GRCGYC 1 cut(s) 312
BssT1I CCWWGG 2 cut(s) 50, 798
Bst2UI CCWGG 2 cut(s) 501, 885
Bst4CI ACNGT 4 cut(s) 146, 454, 757, 812
BstACI GRCGYC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 866
BstC8I GCNNGC 3 cut(s) 231, 351, 720
BstDEI CTNAG 1 cut(s) 636
BstDSI CCRYGG 2 cut(s) 248, 798
BstF5I GGATG 1 cut(s) 816
BstHHI GCGC 2 cut(s) 283, 438
BstKTI GATC 2 cut(s) 421, 893
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 2 cut(s) 418, 890
BstMWI GCNNNNNNNGC 1 cut(s) 866
BstNI CCWGG 2 cut(s) 501, 885
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 3 cut(s) 87, 499, 883
BstV1I GCAGC 1 cut(s) 318
BstV2I GAAGAC 2 cut(s) 333, 723
BstX2I RGATCY 1 cut(s) 890
BstYI RGATCY 1 cut(s) 890
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 1 cut(s) 353
BtgI CCRYGG 2 cut(s) 248, 798
BtsCI GGATG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 384, 475, 573
Cac8I GCNNGC 3 cut(s) 231, 351, 720
CaiI CAGNNNCTG 1 cut(s) 850
CfoI GCGC 2 cut(s) 283, 438
Cfr10I RCCGGY 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 704
CseI GACGC 1 cut(s) 301
Csp6I GTAC 2 cut(s) 783, 803
CviAII CATG 5 cut(s) 550, 607, 622, 727, 799
CviQI GTAC 2 cut(s) 783, 803
DdeI CTNAG 1 cut(s) 636
DpnI GATC 2 cut(s) 420, 892
DpnII GATC 2 cut(s) 418, 890
DraI TTTAAA 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 351
Ecl136II GAGCTC 1 cut(s) 396
Eco130I CCWWGG 2 cut(s) 50, 798
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 704
Eco53kI GAGCTC 1 cut(s) 396
Eco57I CTGAAG 2 cut(s) 519, 915
EcoICRI GAGCTC 1 cut(s) 396
EcoRII CCWGG 2 cut(s) 499, 883
EcoT14I CCWWGG 2 cut(s) 50, 798
EcoT38I GRGCYC 1 cut(s) 398
ErhI CCWWGG 2 cut(s) 50, 798
FaeI CATG 5 cut(s) 553, 610, 625, 730, 802
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 2 cut(s) 420, 515
FatI CATG 5 cut(s) 549, 606, 621, 726, 798
FauI CCCGC 1 cut(s) 889
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 4 cut(s) 104, 307, 310, 372
FokI GGATG 1 cut(s) 803
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 4 cut(s) 104, 307, 310, 372
FspBI CTAG 3 cut(s) 45, 522, 569
GlaI GCGC 2 cut(s) 282, 437
GluI GCNGC 4 cut(s) 104, 307, 310, 372
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 1 cut(s) 353
HapII CCGG 3 cut(s) 88, 303, 459
HgaI GACGC 1 cut(s) 301
HhaI GCGC 2 cut(s) 283, 438
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 5 cut(s) 553, 610, 625, 730, 802
Hin6I GCGC 2 cut(s) 281, 436
HinP1I GCGC 2 cut(s) 281, 436
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 256
HinfI GANTC 6 cut(s) 244, 320, 408, 414, 425, 712
HpaII CCGG 3 cut(s) 88, 303, 459
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 2 cut(s) 316, 753
Hpy188I TCNGA 2 cut(s) 413, 639
Hpy188III TCNNGA 4 cut(s) 241, 265, 422, 537
Hpy8I GTNNAC 2 cut(s) 316, 753
Hpy99I CGWCG 4 cut(s) 317, 320, 337, 340
HpyAV CCTTC 2 cut(s) 543, 623
HpyCH4III ACNGT 4 cut(s) 146, 454, 757, 812
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 271, 695, 860
HpyF10VI GCNNNNNNNGC 1 cut(s) 866
HpyF3I CTNAG 1 cut(s) 636
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 5 cut(s) 553, 610, 625, 730, 802
HspAI GCGC 2 cut(s) 281, 436
KpnI GGTACC 1 cut(s) 786
Kzo9I GATC 2 cut(s) 418, 890
LmnI GCTCC 2 cut(s) 401, 504
Lsp1109I GCAGC 1 cut(s) 318
LweI GCATC 3 cut(s) 258, 621, 692
MaeI CTAG 3 cut(s) 45, 522, 569
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 812, 840
MalI GATC 2 cut(s) 420, 892
MboI GATC 2 cut(s) 418, 890
MboII GAAGA 8 cut(s) 69, 253, 286, 333, 723, 823, 906, 921
MflI RGATCY 1 cut(s) 890
MhlI GDGCHC 1 cut(s) 398
MluCI AATT 3 cut(s) 83, 202, 209
MlyI GAGTC 2 cut(s) 314, 402
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 6 cut(s) 50, 409, 518, 645, 830, 945
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 2 cut(s) 578, 664
MspA1I CMGCKG 1 cut(s) 309
MspI CCGG 3 cut(s) 88, 303, 459
MspR9I CCNGG 3 cut(s) 89, 501, 885
MvaI CCWGG 2 cut(s) 501, 885
MwoI GCNNNNNNNGC 1 cut(s) 866
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 1 cut(s) 798
NdeII GATC 2 cut(s) 418, 890
NlaIII CATG 5 cut(s) 553, 610, 625, 730, 802
NlaIV GGNNCC 3 cut(s) 506, 705, 784
NspI RCATGY 1 cut(s) 730
OliI CACNNNNGTG 2 cut(s) 578, 664
PciI ACATGT 1 cut(s) 726
PcsI WCGNNNNNNNCGW 1 cut(s) 329
PfeI GAWTC 4 cut(s) 244, 414, 425, 712
PfoI TCCNGGA 1 cut(s) 87
PkrI GCNGC 4 cut(s) 105, 308, 311, 373
PleI GAGTC 2 cut(s) 314, 402
PpsI GAGTC 2 cut(s) 314, 402
PscI ACATGT 1 cut(s) 726
Psp124BI GAGCTC 1 cut(s) 398
Psp6I CCWGG 2 cut(s) 499, 883
PspGI CCWGG 2 cut(s) 499, 883
PspN4I GGNNCC 3 cut(s) 506, 705, 784
PspPI GGNCC 1 cut(s) 704
PstNI CAGNNNCTG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 890
RsaI GTAC 2 cut(s) 784, 804
RsaNI GTAC 2 cut(s) 783, 803
RseI CAYNNNNRTG 2 cut(s) 578, 664
SacI GAGCTC 1 cut(s) 398
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 4 cut(s) 104, 307, 310, 372
Sau3AI GATC 2 cut(s) 418, 890
Sau96I GGNCC 1 cut(s) 704
SchI GAGTC 2 cut(s) 314, 402
ScrFI CCNGG 3 cut(s) 89, 501, 885
SduI GDGCHC 1 cut(s) 398
SetI ASST 9 cut(s) 42, 183, 260, 398, 502, 547, 720, 849, 956
SfaNI GCATC 3 cut(s) 258, 621, 692
SgrDI CGTCGACG 1 cut(s) 314
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 2 cut(s) 578, 664
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SpeI ACTAGT 1 cut(s) 521
Sse9I AATT 3 cut(s) 83, 202, 209
SsiI CCGC 5 cut(s) 104, 309, 372, 431, 896
SspMI CTAG 3 cut(s) 45, 522, 569
SstI GAGCTC 1 cut(s) 398
StyD4I CCNGG 3 cut(s) 87, 499, 883
StyI CCWWGG 2 cut(s) 50, 798
TaaI ACNGT 4 cut(s) 146, 454, 757, 812
TaiI ACGT 1 cut(s) 183
TaqI TCGA 4 cut(s) 150, 315, 417, 423
TasI AATT 3 cut(s) 83, 202, 209
TauI GCSGC 3 cut(s) 106, 312, 374
TfiI GAWTC 4 cut(s) 244, 414, 425, 712
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 3 cut(s) 391, 475, 580
TseI GCWGC 1 cut(s) 306
TspDTI ATGAA 2 cut(s) 26, 864
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 3 cut(s) 391, 475, 580
VpaK11BI GGWCC 1 cut(s) 704
XapI RAATTY 1 cut(s) 209
XceI RCATGY 1 cut(s) 730
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 3 cut(s) 45, 522, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.