pycom03g04050

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007364
Physical Location & Seq
Forward (+)
3191135 .. 3191802
668 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 600 bp
ATGACTATTGAAAGCACTTTGTTGTCTATCGAGTGGGGGATCGCCGAGCTAGTGAACCACCCCGACAGCCAGAAGAAGTTGAGGGAAGAACTCGATACTGTCCTCGGCCGTGGAGTACAAATCACAGAGCCCGACATTCAGAAACTTCCCTACCTGCAAGCCGTGATCAAGGAGACTCTCCGCCTTCGCATGACAATTCCACTCCTTCTTCCCCACATGAACCTTAAGGACGCCAAGCTCAGTGGGTTTGACATCCCGGCGGGGAGCAAGATTTTGGTGAATGCTTGGTGGTTGGCGAACAACCCTGCCATGTGGAAGAAGCCGGAGGAGTTTAGGCCCGAGAGGTTTTTGGAGGAGGAGTCGAAGGTGGAGGCTAACGGGAACGACTTCAGGTATCTCCCGTTTGGTGTTGGGAGGAGAAGCTGTCCCGGTATTATCCTGGCCCTGCCAATCCTATGCATTACAATTGGACGTTTAGTTCAGAACTTCGAGCTTCTACCTCCGCCGGGACAGTCCAAGCTCGACACCTCGGAGAAATGCGGGCAGTTCAGCTTGCACATCCTGAAGCATTCCACCATTGTGATGAAGCCGACGACATAG

Protein Analysis

200

Amino Acids

22.49

Weight (kDa)

7.73

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 4 - 191 7.3e-64 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 162
AciI CCGC 4 cut(s) 181, 260, 503, 540
AclWI GGATC 1 cut(s) 47
AcoI YGGCCR 1 cut(s) 106
AcuI CTGAAG 2 cut(s) 373, 584
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 1 cut(s) 506
AflII CTTAAG 1 cut(s) 224
AgsI TTSAA 1 cut(s) 11
AjnI CCWGG 1 cut(s) 438
AleI CACNNNNGTG 1 cut(s) 578
AloI GAACNNNNNNTCC 2 cut(s) 462, 494
AluBI AGCT 6 cut(s) 49, 238, 423, 493, 520, 552
AluI AGCT 6 cut(s) 49, 238, 423, 493, 520, 552
Alw26I GTCTC 1 cut(s) 167
AlwI GGATC 1 cut(s) 47
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 3 cut(s) 106, 335, 441
Asp700I GAANNNNTTC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 336, 442
AsuC2I CCSGG 3 cut(s) 257, 429, 507
AsuHPI GGTGA 1 cut(s) 289
AvaI CYCGRG 1 cut(s) 338
BanII GRGCYC 1 cut(s) 132
BceAI ACGGC 2 cut(s) 93, 146
BciT130I CCWGG 1 cut(s) 440
BclI TGATCA 1 cut(s) 165
BcnI CCSGG 3 cut(s) 257, 429, 507
BcoDI GTCTC 1 cut(s) 167
BfaI CTAG 1 cut(s) 50
BfrI CTTAAG 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 162
Bme1390I CCNGG 4 cut(s) 257, 429, 440, 507
BmeT110I CYCGRG 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 336, 442
BmrFI CCNGG 4 cut(s) 257, 429, 440, 507
BpuMI CCSGG 3 cut(s) 257, 429, 507
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 103, 109, 528
BsaXI ACNNNNNCTCC 4 cut(s) 344, 374, 409, 439
Bsc4I CCNNNNNNNGG 1 cut(s) 506
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 3 cut(s) 103, 109, 528
BseGI GGATG 2 cut(s) 252, 558
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMII CTCAG 1 cut(s) 253
BseRI GAGGAG 4 cut(s) 341, 368, 371, 430
BseX3I CGGCCG 1 cut(s) 106
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 108, 337, 443
BsiEI CGRYCG 1 cut(s) 109
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 4 cut(s) 257, 323, 429, 506
BslFI GGGAC 2 cut(s) 411, 522
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 1 cut(s) 167
BsmFI GGGAC 2 cut(s) 411, 522
BsmI GAATGC 2 cut(s) 286, 568
BsnI GGCC 3 cut(s) 108, 337, 443
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 39, 165
BspACI CCGC 4 cut(s) 181, 260, 503, 540
BspANI GGCC 3 cut(s) 108, 337, 443
BspCNI CTCAG 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 162
BspPI GGATC 1 cut(s) 47
BspTI CTTAAG 1 cut(s) 224
BssECI CCNNGG 3 cut(s) 103, 109, 528
BssMI GATC 2 cut(s) 39, 165
BssNI GRCGYC 1 cut(s) 231
Bst2UI CCWGG 1 cut(s) 440
Bst4CI ACNGT 2 cut(s) 100, 513
BstACI GRCGYC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 159, 542, 554
BstDEI CTNAG 1 cut(s) 239
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 2 cut(s) 252, 558
BstKTI GATC 2 cut(s) 42, 168
BstMAI GTCTC 1 cut(s) 167
BstMBI GATC 2 cut(s) 39, 165
BstMCI CGRYCG 1 cut(s) 109
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 4 cut(s) 255, 427, 438, 505
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 3 cut(s) 108, 337, 443
BtgI CCRYGG 1 cut(s) 109
BtsCI GGATG 2 cut(s) 252, 558
BtsIMutI CAGTG 1 cut(s) 247
BveI ACCTGC 1 cut(s) 162
Cac8I GCNNGC 3 cut(s) 159, 542, 554
Cfr13I GGNCC 2 cut(s) 336, 442
CseI GACGC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 116
CspCI CAANNNNNGTGG 2 cut(s) 223, 258
CviAII CATG 3 cut(s) 190, 217, 310
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 1 cut(s) 239
DpnI GATC 2 cut(s) 41, 167
DpnII GATC 2 cut(s) 39, 165
EaeI YGGCCR 1 cut(s) 106
EagI CGGCCG 1 cut(s) 106
EciI GGCGGA 2 cut(s) 170, 492
EclXI CGGCCG 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 132
Eco52I CGGCCG 1 cut(s) 106
Eco57I CTGAAG 2 cut(s) 373, 584
Eco88I CYCGRG 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 461
EcoT38I GRGCYC 1 cut(s) 132
FaeI CATG 3 cut(s) 193, 220, 313
FaiI YATR 5 cut(s) 191, 218, 311, 457, 598
FaqI GGGAC 2 cut(s) 411, 522
FatI CATG 3 cut(s) 189, 216, 309
FauI CCCGC 2 cut(s) 253, 533
FbaI TGATCA 1 cut(s) 165
FokI GGATG 2 cut(s) 239, 545
FriOI GRGCYC 1 cut(s) 132
FspBI CTAG 1 cut(s) 50
HaeIII GGCC 3 cut(s) 108, 337, 443
HapII CCGG 4 cut(s) 257, 323, 429, 506
HgaI GACGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 3 cut(s) 193, 220, 313
HinfI GANTC 2 cut(s) 175, 359
HpaII CCGG 4 cut(s) 257, 323, 429, 506
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 141, 483, 532
Hpy188III TCNNGA 1 cut(s) 562
Hpy8I GTNNAC 1 cut(s) 55
Hpy99I CGWCG 1 cut(s) 595
HpyAV CCTTC 3 cut(s) 194, 215, 358
HpyCH4III ACNGT 2 cut(s) 100, 513
HpyCH4IV ACGT 1 cut(s) 472
HpyCH4V TGCA 3 cut(s) 157, 459, 556
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 472
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 3 cut(s) 193, 220, 313
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 2 cut(s) 39, 165
LmnI GCTCC 1 cut(s) 264
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 472
MalI GATC 2 cut(s) 41, 167
MboI GATC 2 cut(s) 39, 165
MboII GAAGA 4 cut(s) 85, 98, 200, 328
MfeI CAATTG 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 2 cut(s) 195, 465
MlyI GAGTC 2 cut(s) 169, 368
Mph1103I ATGCAT 1 cut(s) 461
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 1 cut(s) 225
MslI CAYNNNNRTG 2 cut(s) 578, 581
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 4 cut(s) 257, 323, 429, 506
MspR9I CCNGG 4 cut(s) 257, 429, 440, 507
MunI CAATTG 1 cut(s) 465
Mva1269I GAATGC 2 cut(s) 286, 568
MvaI CCWGG 1 cut(s) 440
NciI CCSGG 3 cut(s) 257, 429, 507
NdeII GATC 2 cut(s) 39, 165
NlaIII CATG 3 cut(s) 193, 220, 313
NmeAIII GCCGAG 2 cut(s) 70, 84
NsiI ATGCAT 1 cut(s) 461
OliI CACNNNNGTG 1 cut(s) 578
PctI GAATGC 2 cut(s) 286, 568
PdmI GAANNNNTTC 1 cut(s) 386
PleI GAGTC 2 cut(s) 169, 367
PpsI GAGTC 2 cut(s) 169, 367
Psp6I CCWGG 1 cut(s) 438
PspGI CCWGG 1 cut(s) 438
PspPI GGNCC 2 cut(s) 336, 442
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 2 cut(s) 578, 581
SaqAI TTAA 1 cut(s) 225
Sau3AI GATC 2 cut(s) 39, 165
Sau96I GGNCC 2 cut(s) 336, 442
SchI GAGTC 2 cut(s) 169, 368
ScrFI CCNGG 4 cut(s) 257, 429, 440, 507
SduI GDGCHC 1 cut(s) 132
SmiMI CAYNNNNRTG 2 cut(s) 578, 581
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 2 cut(s) 195, 465
SsiI CCGC 4 cut(s) 181, 260, 503, 540
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 4 cut(s) 255, 427, 438, 505
TaaI ACNGT 2 cut(s) 100, 513
TaiI ACGT 1 cut(s) 475
TaqI TCGA 5 cut(s) 30, 93, 362, 489, 522
TasI AATT 2 cut(s) 195, 465
TatI WGTACW 1 cut(s) 115
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 233, 599
TspRI CASTG 1 cut(s) 247
Vha464I CTTAAG 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.